Chen, W.; Chen, R.; Liu, Q.; He, Y.; He, K. et al.: Orange, red, yellow: Biosynthesis of azaphilone pigments in Monascus fungi. In: Chemical Science 8 (2017), Nr. 7, S. 4917-4925. DOI:
https://doi.org/10.1039/c7sc00475c
Abstract: |
Monascus azaphilone pigments (MonAzPs) are very widely used as food colorants, but their biosynthetic pathway has remained poorly characterized for more than half a century. In this study, the individual steps of MonAzPs biosynthesis in Monascus ruber M7 were elucidated by a combination of targeted gene knockouts, heterologous gene expression, and in vitro chemical and enzymatic reactions. This study describes the first rational engineering of MonAzPs biosynthesis and provides a roadmap for future pathway engineering efforts directed towards the selective production of the most valuable pigments and serves as a model for the biosynthesis of fungal azaphilones in general.
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License of this version: |
CC BY-NC 3.0 Unported - https://creativecommons.org/licenses/by-nc/3.0/
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Publication type: |
Article |
Publishing status: |
publishedVersion |
Publication date: |
2017 |
Keywords english: |
Biochemistry, Gene expression, Genes, Biosynthetic pathway, Enzymatic reaction, Food colorants, Gene knockout, Heterologous gene expressions, In-vitro, Pathway engineerings, Selective production, Biosynthesis
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DDC: |
540 | Chemie
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