Zusammenfassung: | |
Two-photon polymerization (2PP) is applied for the fabrication of 3-D Zr-Si scaffolds for bone tissue engineering. Zr-Si scaffolds with 150, 200, and 250 μm pore sizes are seeded with human bone marrow stem cells (hBMSCs) and human adipose tissue derived stem cells (hASCs) and cultured in osteoinductive and control media for three weeks. Osteogenic differentiation of hASCs and hBMSCs and formation of bone matrix is comparatively analyzed via alkaline phosphatase activity (ALP), calcium quantification, osteocalcin staining and scanning electron microscopy (SEM). It is observed that the 150 μm pore size Zr-Si scaffolds support the strongest matrix mineralization, as confirmed by calcium deposition. Analysis of ALP activity, osteocalcin staining and SEM observations of matrix mineralization reveal that mesenchymal stem cells cultured on 3-D scaffolds without osteogenic stimulation spontaneously differentiate towards osteogenic lineage. Nanoindentation measurements show that aging of the 2PP-produced Zr-Si scaffolds in aqueous or alcohol media results in an increase in the scaffold Young's modulus and hardness. Moreover, accelerated formation of bone matrix by hASCs is noted, when cultured on the scaffolds with lower Young's moduli and hardness values (non aged scaffolds) compared to the cells cultured on scaffolds with higher Young's modulus and hardness values (aged scaffolds). Presented results support the potential application of Zr-Si scaffolds for autologous bone tissue engineering.
|
|
Lizenzbestimmungen: | CC BY 4.0 Unported - https://creativecommons.org/licenses/by/4.0/ |
Publikationstyp: | Article |
Publikationsstatus: | publishedVersion |
Erstveröffentlichung: | 2015 |
Schlagwörter (englisch): | alcohol, alkaline phosphatase, calcium ion, molecular scaffold, osteocalcin, polymer, silicon, zirconium, alkaline phosphatase, calcium, inorganic compound, organic compound, osteocalcin, silicon, zirconium, adipose derived stem cell, aging, aqueous solution, Article, bone development, bone marrow derived mesenchymal stem cell, bone matrix, bone mineralization, bone tissue, calcium bone level, calcium cell level, cell differentiation, cell labeling, cell lineage, composite material, controlled study, culture medium, hardness, human, human cell, mesenchymal stem cell, microtechnology, ossification, polymerization, scanning electron microscopy, staining, stem cell culture, synthesis, tissue engineering, tissue scaffold, two photon polymerization, Young modulus, adipose tissue, bone development, cell culture, cell culture technique, cell proliferation, chemistry, cytology, mesenchymal stroma cell, metabolism, polymerization, procedures, reproducibility, stem cell, time factor, tissue engineering, tissue scaffold, ultrastructure, Adipose Tissue, Alkaline Phosphatase, Calcium, Cell Culture Techniques, Cell Differentiation, Cell Proliferation, Cells, Cultured, Chemistry Techniques, Synthetic, Elastic Modulus, Hardness, Humans, Inorganic Chemicals, Mesenchymal Stromal Cells, Microscopy, Electron, Scanning, Organic Chemicals, Osteocalcin, Osteogenesis, Polymerization, Reproducibility of Results, Silicon, Stem Cells, Time Factors, Tissue Engineering, Tissue Scaffolds, Zirconium |
Fachliche Zuordnung (DDC): | 500 | Naturwissenschaften, 610 | Medizin, Gesundheit |
Anzeige der Dokumente mit ähnlichem Titel, Autor, Urheber und Thema.