Non-Invasive Online Monitoring of Cell Growth in Disposable Bioreactors with a Planar Coil

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dc.identifier.uri http://dx.doi.org/10.15488/2610
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/2636
dc.contributor.author Reinecke, T.
dc.contributor.author Biechele, P.
dc.contributor.author Frickhöffer, M.
dc.contributor.author Scheper, T.
dc.contributor.author Zimmermann, S.
dc.date.accessioned 2018-01-19T08:57:25Z
dc.date.available 2018-01-19T08:57:25Z
dc.date.issued 2016
dc.identifier.citation Reinecke, T.; Biechele, P.; Frickhöffer, M.; Scheper, T.; Zimmermann, S.: Non-Invasive Online Monitoring of Cell Growth in Disposable Bioreactors with a Planar Coil. In: Procedia Engineering 168 (2016), S. 582-585. DOI: https://doi.org/10.1016/j.proeng.2016.11.219
dc.description.abstract To ensure high quality output of biotechnological processes, relevant process parameters need to be monitored. As bioprocesses are increasingly executed in single use bioreactors, there is an increasing demand for new sensors applicable to these processes. In this work, we present a low-cost sensor system for continuous non-invasive cell growth monitoring, especially for single use bioreactor applications. The system consists of a planar coil connected to a low cost network analyzer. The coil is attached to the outside of the polymer foil of the single use bioreactor and an impedance spectrum is measured. To evaluate the sensor, E. coli cultivations are performed in a modified cultivation setup, which enables measurements through the polymer foil of a Sartorius BIOSTAT® CultiBag RM, and additionally allows sampling of culture medium for optical density reference measurements. The resonance peak of the coil in the impedance spectrum, is observed as measure for the optical density. Regardless of the simple sensor construction, we found a good correlation between optical density and the damping ratio of the resonance peak. eng
dc.language.iso eng
dc.publisher Amsterdam : Elsevier
dc.relation.ispartofseries Procedia Engineering 168 (2016)
dc.rights CC BY-NC-ND 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject cell growth monitoring eng
dc.subject single use bioreactor eng
dc.subject inductive permittivity measurements eng
dc.subject dielectric spectroscopy eng
dc.subject.ddc 620 | Ingenieurwissenschaften und Maschinenbau ger
dc.title Non-Invasive Online Monitoring of Cell Growth in Disposable Bioreactors with a Planar Coil
dc.type article
dc.type conferenceObject
dc.type Text
dc.relation.issn 18777058
dc.relation.doi https://doi.org/10.1016/j.proeng.2016.11.219
dc.bibliographicCitation.volume 168
dc.bibliographicCitation.firstPage 582
dc.bibliographicCitation.lastPage 585
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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