Study of Protein Adsorption During Sterile Filtration of Protein Formulations by ILC

Original version

Haindl, S.M.H.; Doppleb, O.; Förster, L.; Wraage, S.; Reiche, A.: Study of Protein Adsorption During Sterile Filtration of Protein Formulations by ILC. In: Chemie-Ingenieur-Technik 92 (2020), Nr. 8, S. 1109-1121. DOI: https://doi.org/10.1002/cite.201900185

Repository version

To cite the version in the repository, please use this identifier: https://doi.org/10.15488/10633

Files in this item

Thumbnail
Name: Study of Protein ...
Size: 908.8Kb
Format: PDF
View/Open
Abstract:
Protein adsorption is usually regarded as the main reason for filter fouling in sterile filtration of protein formulations. To achieve a better insight into this phenomenon, protein adsorption was studied during filtration of stabilized bovine serum albumin (BSA) and γ‐globulin formulations through 0.2-µm microfilter membranes by inverse liquid chromatography (ILC). Adsorption processes can be studied with this method by measurement of breakthrough curves. The change of the concentration in the fluid phase is measured with high accuracy by an inline UV-detector. © 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
License of this version: CC BY 4.0 Unported - https://creativecommons.org/licenses/by/4.0/
Publication type: Article
Publishing status: publishedVersion
Publication date: 2020
Keywords english: inverse liquid chromatography, protein adsorption, sterile filtration
DDC: 540 | Chemie

Usage Statistics

Show full item record

This item appears in the following Collection(s):

 

Search the repository


Browse

My Account

Usage Statistics