Walter, Johanna-Gabriela; Eilers, Alina; Alwis, Lourdes Shanika Malindi; Roth, Bernhard Wilhelm; Bremer, Kort: SPR Biosensor Based on Polymer Multi-Mode Optical Waveguide and Nanoparticle Signal Enhancement. In: Sensors 20 (2020), Nr. 10, 2889. DOI:
https://doi.org/10.3390/s20102889
Zusammenfassung: |
We present a surface plasmon resonance (SPR) biosensor that is based on a planar-optical multi-mode (MM) polymer waveguide structure applied for the detection of biomolecules in the lower nano-molar (nM) range. The basic sensor shows a sensitivity of 608.6 nm/RIU when exposed to refractive index changes with a measurement resolution of 4.3 × 10−3 RIU. By combining the SPR sensor with an aptamer-functionalized, gold-nanoparticle (AuNP)-enhanced sandwich assay, the detection of C-reactive protein (CRP) in a buffer solution was achieved with a response of 0.118 nm/nM. Due to the multi-mode polymer waveguide structure and the simple concept, the reported biosensor is well suited for low-cost disposable lab-on-a-chip applications and can be used with rather simple and economic devices. In particular, the sensor offers the potential for fast and multiplexed detection of several biomarkers on a single integrated platform.
|
Lizenzbestimmungen: |
CC BY 4.0 Unported - https://creativecommons.org/licenses/by/4.0/
|
Publikationstyp: |
Article |
Publikationsstatus: |
publishedVersion |
Erstveröffentlichung: |
2020 |
Schlagwörter (englisch): |
surface plasmon resonance (SPR), sensor, planar-optical, multi-mode, waveguide, biosensor, aptamer, gold-nanoparticle, lab-on-a-chip
|
Fachliche Zuordnung (DDC): |
620 | Ingenieurwissenschaften und Maschinenbau
|