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3D printing in biotechnology—An insight into miniaturized and microfluidic systems for applications from cell culture to bioanalytics

Heuer, Christopher; Preuß, John-Alexander; Habib, Taieb; Enders, Anton; Bahnemann, Janina (Weinheim : Wiley-VCH, 2021)

Since its invention in the 1980s, 3D printing has evolved into a versatile technique for the additive manufacturing of diverse objects and tools, using various materials. The relative flexibility, straightforwardness, and ...
Carbonic anhydrase subunits form a matrix-exposed domain attached to the membrane arm of mitochondrial complex I in plants

Sunderhaus, Stephanie; Dudkina, Natalya V.; Jänsch, Lothar; Klodmann, Jennifer; Heinemeyer, Jesco; Perales, Mariano; Zabaleta, Eduardo; Boekema, Egbert J.; Braun, Hans-Peter (Bethesda, Md. : ASBMB Publications, 2006)

Complex I of Arabidopsis includes five structurally related subunits representing γ-type carbonic anhydrases termed CA1, CA2, CA3, CAL1, and CAL2. The position of these subunits within complex I was investigated. Direct ...
Continuous optical in-line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality

Lederle, Mario; Tric, Mircea; Roth, Tatjana; Schütte, Lina; Rattenholl, Anke; Lütkemeyer, Dirk; Wölfl, Stefan; Werner, Tobias; Wiedemann, Philipp (Weinheim : Wiley-VCH, 2021)

Great efforts are directed towards improving productivity, consistency and quality of biopharmaceutical processes and products. One particular area is the development of new sensors for continuous monitoring of critical ...
Effects of degradable Mg-Ca alloys on dendritic cell function

Feser, K.; Kietzmann, M.; Bäumer, W.; Krause, C.; Bach, F.W. (London : SAGE Publications Ltd., 2011)

Degradable magnesium alloys are new materials for implants used in orthopedic and trauma surgery. The aim of this study was to investigate the influence of degradable magnesium alloys on the function of dendritic cells ...
Entwicklung eines automatisierten, Mikrotiterplatten basierten Systems für die Suspensions-Zellkultur zur Effizienzsteigerung der Prozessentwicklung im pharmazeutisch industriellen Umfeld

Markert, Sven (Hannover : Gottfried Wilhelm Leibniz Universität Hannover, 2012)

[no abstract]
Entwicklung und Anwendung eines modularen Bioreaktors mit tubulären Membranen

Wolff, Christoph Berend (Hannover : Gottfried Wilhelm Leibniz Universität Hannover, 2013)

[no abstract]
Entwicklung und Automatisierung 3D-gedruckter mikrofluidischer Systeme zur Integration und Kultivierung adhärenter Zellkulturen

Winkler, Steffen Nils (Hannover : Institutionelles Repositorium der Leibniz Universität Hannover, 2022)

Mikrofluidische Systeme werden zur Manipulation von Flüssigkeiten auf Mikroebene eingesetzt. Von ihnen profitieren insbesondere Biowissenschaften durch die Reduktion von Reagenzien und die Automatisierung ganzer Arbeitsabläufe. ...
In vitro biocompatibility evaluation of a heat-resistant 3D printing material for use in customized cell culture devices

Winkler, Steffen; Meyer, Katharina V.; Heuer, Christopher; Kortmann, Carlotta; Dehne, Michaela; Bahnemann, Janina (Weinheim : Wiley-VCH, 2022)

Additive manufacturing (3D printing) enables the fabrication of highly customized and complex devices and is therefore increasingly used in the field of life sciences and biotechnology. However, the application of 3D-printed ...
Inner tegument proteins of Herpes Simplex Virus are sufficient for intracellular capsid motility in neurons but not for axonal targeting

Buch, Anna; Müller, Oliver; Ivanova, Lyudmila; Döhner, Katinka; Bialy, Dagmara; Bosse, Jens B.; Pohlmann, Anja; Binz, Anne; Hegemann, Maike; Nagel, Claus-Henning; Koltzenburg, Martin; Viejo-Borbolla, Abel; Rosenhahn, Bodo; Bauerfeind, Rudolf; Sodeik, Beate (San Francisco, CA : Public Library of Science (PLoS), 2017)

Upon reactivation from latency and during lytic infections in neurons, alphaherpesviruses assemble cytosolic capsids, capsids associated with enveloping membranes, and transport vesicles harboring fully enveloped capsids. ...
Osteogenic differentiation of human mesenchymal stem cells in 3-D Zr-Si organic-inorganic scaffolds produced by two-photon polymerization technique

Koroleva, Anastasia; Deiwick, Andrea; Nguyen, Alexander; Schlie-Wolter, Sabrina; Narayan, Roger; Timashev, Peter; Popov, Vladimir; Bagratashvili, Victor N.; Chichkov, Boris (San Francisco, CA : Public Library of Science, 2015)

Two-photon polymerization (2PP) is applied for the fabrication of 3-D Zr-Si scaffolds for bone tissue engineering. Zr-Si scaffolds with 150, 200, and 250 μm pore sizes are seeded with human bone marrow stem cells (hBMSCs) ...
Pomegranate (Punica granatum L.) Extract and Its Anthocyanin and Copigment Fractions—Free Radical Scavenging Activity and Influence on Cellular Oxidative Stress

Kostka, Tina; Ostberg-Potthoff, Johanna Josefine; Briviba, Karlis; Matsugu, Seiichi; Winterhalter, Peter; Esatbeyoglu, Tuba (Basel : MDPI AG, 2020)

Secondary plant metabolites, e.g., polyphenols, are widely known as health-improving compounds that occur in natural functional foods such as pomegranates. While extracts generated from these fruits inhibit oxidative stress, ...
A practical synthesis of Rho-Kinase inhibitor Y-27632 and fluoro derivatives and their evaluation in human pluripotent stem cells

Paleek, Jiri; Zweigerdt, Robert; Olmer, Ruth; Martin, Ulrich; Kirschning, Andreas; Dräger, Gerald (Cambridge : Royal Society of Chemistry, 2011)

A practical synthesis of the Rho-Kinase inhibitor Y-27632 and two new fluoro derivatives was achieved in seven steps and with a good overall yield of 45% starting from commercially available (R)-1-phenylethylamine. Compared ...
Role of gamma-secretase in human umbilical-cord derived mesenchymal stem cell mediated suppression of NK cell cytotoxicity

Chatterjee, Debanjana; Marquardt, Nicole; Tufa, Dejene Milkessa; Beauclair, Guillaume; Low, Hui Zhi; Hatlapatka, Tim; Hass, Ralf; Kasper, Cornelia; Kaisenberg, Constantin von; Schmidt, Reinhold Ernst; Jacobs, Roland (London : BioMed Central Ltd., 2014)

Background: Mesenchymal stem cells (MSCs) are increasingly considered to be used as biological immunosuppressants in hematopoietic stem cell transplantation (HSCT). In the early reconstitution phase following HSCT, natural ...
Sensors and Techniques for On-Line Determination of Cell Viability in Bioprocess Monitoring

Rösner, Laura S.; Walter, Franziska; Ude, Christian; John, Gernot T.; Beutel, Sascha (Basel : MDPI, 2022)

In recent years, the bioprocessing industry has experienced significant growth and is increasingly emerging as an important economic sector. Here, efficient process management and constant control of cellular growth are ...

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