Identification of genes encoding squalestatin S1 biosynthesis and in vitro production of new squalestatin analogues

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dc.identifier.uri http://dx.doi.org/10.15488/791
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/815
dc.contributor.author Bonsch, B.
dc.contributor.author Belt, V.
dc.contributor.author Bartel, C.
dc.contributor.author Duensing, N.
dc.contributor.author Koziol, M.
dc.contributor.author Lazarus, Colin M.
dc.contributor.author Bailey, Andrew M.
dc.contributor.author Simpson, Thomas J.
dc.contributor.author Cox, Russell J.
dc.date.accessioned 2016-12-06T07:33:00Z
dc.date.available 2016-12-06T07:33:00Z
dc.date.issued 2016
dc.identifier.citation Bonsch, B.; Belt, V.; Bartel, C.; Duensing, N.; Koziol, M.et al.: Identification of genes encoding squalestatin S1 biosynthesis and in vitro production of new squalestatin analogues. In: Chemical communications 52 (2016), Nr. 42, S. 6777-6780. DOI: http://dx.doi.org/10.1039/C6CC02130A
dc.description.abstract A gene cluster responsible for the biosynthesis of squalestatin S1 (SQS1, 1) was identified by full genome sequencing of two SQS1-producing ascomycetes: Phoma sp. C2932 and unidentified fungus MF5453. A transformation protocol was established and a subsequent knockout of one PKS gene from the cluster led to loss of SQS1 production and enhanced concentration of an SQS1 precursor. An acyltransferase gene from the cluster was expressed in E. coli and the expressed protein MfM4 shown to be responsible for loading acyl groups from CoA onto the squalestatin core as the final step of biosynthesis. MfM4 appears to have a broad substrate selectivity for its acyl CoA substrate, allowing the in vitro synthesis of novel squalestatins. eng
dc.language.iso eng
dc.publisher Cambridge : Royal Society of Chemistry
dc.relation.ispartofseries Chemical communications 52 (2016), Nr. 42
dc.rights CC BY 3.0
dc.rights.uri https://creativecommons.org/licenses/by/3.0/
dc.subject gene cluster eng
dc.subject SQS1 eng
dc.subject Phoma sp. C2932 eng
dc.subject MF5453 eng
dc.subject.ddc 540 | Chemie ger
dc.title Identification of genes encoding squalestatin S1 biosynthesis and in vitro production of new squalestatin analogues
dc.type article
dc.type Text
dc.relation.essn 1364-548X
dc.relation.issn 1359-7345
dc.relation.doi http://dx.doi.org/10.1039/C6CC02130A
dc.bibliographicCitation.issue 41
dc.bibliographicCitation.volume 52
dc.bibliographicCitation.firstPage 6777
dc.bibliographicCitation.lastPage 6780
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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