Identification of genes encoding squalestatin S1 biosynthesis and in vitro production of new squalestatin analogues

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Bonsch, B.; Belt, V.; Bartel, C.; Duensing, N.; Koziol, M.et al.: Identification of genes encoding squalestatin S1 biosynthesis and in vitro production of new squalestatin analogues. In: Chemical communications 52 (2016), Nr. 42, S. 6777-6780. DOI: http://dx.doi.org/10.1039/C6CC02130A

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Abstract: 
A gene cluster responsible for the biosynthesis of squalestatin S1 (SQS1, 1) was identified by full genome sequencing of two SQS1-producing ascomycetes: Phoma sp. C2932 and unidentified fungus MF5453. A transformation protocol was established and a subsequent knockout of one PKS gene from the cluster led to loss of SQS1 production and enhanced concentration of an SQS1 precursor. An acyltransferase gene from the cluster was expressed in E. coli and the expressed protein MfM4 shown to be responsible for loading acyl groups from CoA onto the squalestatin core as the final step of biosynthesis. MfM4 appears to have a broad substrate selectivity for its acyl CoA substrate, allowing the in vitro synthesis of novel squalestatins.
License of this version: CC BY 3.0
Document Type: article
Publishing status: publishedVersion
Issue Date: 2016
Appears in Collections:Naturwissenschaftliche Fakultät

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1 image of flag of Germany Germany 70 89.74%
2 image of flag of China China 5 6.41%
3 image of flag of United States United States 1 1.28%
4 image of flag of Pakistan Pakistan 1 1.28%
5 image of flag of Belgium Belgium 1 1.28%

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