Longitudinal claudin gene expression analyses in canine mammary tissues and thereof derived primary cultures and cell lines

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dc.identifier.uri http://dx.doi.org/10.15488/732
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/756
dc.contributor.author Hammer, Susanne C.
dc.contributor.author Becker, Annegret
dc.contributor.author Rateitschak, Katja
dc.contributor.author Mohr, Annika
dc.contributor.author Ripoli, Florenza Lüder
dc.contributor.author Hennecke, Silvia
dc.contributor.author Junginger, Johannes
dc.contributor.author Hewicker-Trautwein, Marion
dc.contributor.author Brenig, Bertram
dc.contributor.author Ngezahayo, Anaclet
dc.contributor.author Nolte, Ingo
dc.contributor.author Escobar, Hugo Murua
dc.date.accessioned 2016-11-25T08:33:29Z
dc.date.available 2016-11-25T08:33:29Z
dc.date.issued 2016
dc.identifier.citation Hammer, S.C.; Becker, Annegret; Rateitschak, K.; Mohr, A.; Ripoli, F.L. et al.: Longitudinal claudin gene expression analyses in canine mammary tissues and thereof derived primary cultures and cell lines. In: International Journal of Molecular Sciences 17 (2016), Nr. 10 , 1655. DOI: http://dx.doi.org/10.3390/ijms17101655
dc.description.abstract Human and canine mammary tumours show partial claudin expression deregulations. Further, claudins have been used for directed therapeutic approaches. However, the development of claudin targeting approaches requires stable claudin expressing cell lines. This study reports the establishment and characterisation of canine mammary tissue derived cell lines, analysing longitudinally the claudin-1, -3, -4 and -7 expressions in original tissue samples, primary cultures and developed cell lines. Primary cultures were derived from 17 canine mammary tissues: healthy, lobular hyperplasia, simple adenoma, complex adenoma, simple tubular carcinoma, complex carcinoma, carcinoma arising in a benign mixed tumour and benign mixed tissue. Cultivation was performed, if possible, until passage 30. Claudin mRNA and protein expressions were analysed by PCR, QuantiGene Plex Assay, immunocytochemistry and immunofluorescence. Further, cytokeratin expression was analysed immunocytochemically. Cultivation resulted in 11 established cell lines, eight showing epithelial character. In five of the early passages the claudin expressions decreased compared to the original tissues. In general, claudin expressions were diminished during cultivation. Three cell lines kept longitudinally claudin, as well as epithelial marker expressions, representing valuable tools for the development of claudin targeted anti-tumour therapies. eng
dc.language.iso eng
dc.publisher Basel : MDPI AG
dc.relation.ispartofseries International Journal of Molecular Sciences 17 (2016), Nr. 10
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Canine eng
dc.subject Cell culture eng
dc.subject Cell lines eng
dc.subject Claudin eng
dc.subject Mammary neoplasias eng
dc.subject Marker expression eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Longitudinal claudin gene expression analyses in canine mammary tissues and thereof derived primary cultures and cell lines
dc.type article
dc.type Text
dc.relation.issn 1661-6596
dc.relation.doi http://dx.doi.org/10.3390/ijms17101655
dc.bibliographicCitation.issue 10
dc.bibliographicCitation.volume 17
dc.bibliographicCitation.firstPage 1655
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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