Longitudinal claudin gene expression analyses in canine mammary tissues and thereof derived primary cultures and cell lines

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Hammer, S.C.; Becker, Annegret; Rateitschak, K.; Mohr, A.; Ripoli, F.L. et al.: Longitudinal claudin gene expression analyses in canine mammary tissues and thereof derived primary cultures and cell lines. In: International Journal of Molecular Sciences 17 (2016), Nr. 10 , 1655. DOI: http://dx.doi.org/10.3390/ijms17101655

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Abstract: 
Human and canine mammary tumours show partial claudin expression deregulations. Further, claudins have been used for directed therapeutic approaches. However, the development of claudin targeting approaches requires stable claudin expressing cell lines. This study reports the establishment and characterisation of canine mammary tissue derived cell lines, analysing longitudinally the claudin-1, -3, -4 and -7 expressions in original tissue samples, primary cultures and developed cell lines. Primary cultures were derived from 17 canine mammary tissues: healthy, lobular hyperplasia, simple adenoma, complex adenoma, simple tubular carcinoma, complex carcinoma, carcinoma arising in a benign mixed tumour and benign mixed tissue. Cultivation was performed, if possible, until passage 30. Claudin mRNA and protein expressions were analysed by PCR, QuantiGene Plex Assay, immunocytochemistry and immunofluorescence. Further, cytokeratin expression was analysed immunocytochemically. Cultivation resulted in 11 established cell lines, eight showing epithelial character. In five of the early passages the claudin expressions decreased compared to the original tissues. In general, claudin expressions were diminished during cultivation. Three cell lines kept longitudinally claudin, as well as epithelial marker expressions, representing valuable tools for the development of claudin targeted anti-tumour therapies.
License of this version: CC BY 4.0 Unported
Document Type: article
Publishing status: publishedVersion
Issue Date: 2016
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
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1 image of flag of Germany Germany 136 87.74%
2 image of flag of China China 9 5.81%
3 image of flag of United States United States 5 3.23%
4 image of flag of Vietnam Vietnam 2 1.29%
5 image of flag of Russian Federation Russian Federation 1 0.65%
6 image of flag of Qatar Qatar 1 0.65%
7 image of flag of United Kingdom United Kingdom 1 0.65%

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