Regulation of lipid metabolism-related gene expression in whole blood cells of normo- and dyslipidemic men after fish oil supplementation

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dc.identifier.uri http://dx.doi.org/10.15488/650
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/674
dc.contributor.author Schmidt, Simone
dc.contributor.author Willers, Janina
dc.contributor.author Stahl, Frank
dc.contributor.author Mutz, Kai-Oliver
dc.contributor.author Scheper, Thomas
dc.contributor.author Hahn, Andreas
dc.contributor.author Schuchardt, Jan Philipp
dc.date.accessioned 2016-11-03T12:40:19Z
dc.date.available 2016-11-03T12:40:19Z
dc.date.issued 2012
dc.identifier.citation Schmidt, Simone; Willers, Janina; Stahl, Frank; Mutz, Kai-Oliver; Scheper, Thomas et al.: Regulation of lipid metabolism-related gene expression in whole blood cells of normo- and dyslipidemic men after fish oil supplementation. In: Lipids in Health and Disease 11 (2012), 172. DOI: http://dx.doi.org/10.1186/1476-511X-11-172
dc.description.abstract Background: Beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on the lipid levels of dyslipidemic subjects are widely described in the literature. However, the underlying molecular mechanisms are largely unknown. The aim of this study was to investigate the effects of n-3 PUFAs on the expression of lipid metabolism-related genes in normo- and dyslipidemic men to unveil potential genes and pathways affecting lipid metabolism. Methods. Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with six fish oil capsules per day, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. The gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction. Results: Several transcription factors (peroxisome proliferator-activated receptor α (PPARα), retinoid X receptor (RXR) α, RXRγ, hepatic nuclear factor (HNF) 6, and HNF1ß) as well as other genes related to triacylglycerol (TG) synthesis or high-density lipoprotein (HDL-C) and cholesterol metabolism (phospholipids transfer protein, ATP-binding cassette sub-family G member 5, 2-acylglycerol O-acyltransferase (MOGAT) 3, MOGAT2, diacylglycerol O-acyltransferase 1, sterol O-acyltransferase 1, apolipoprotein CII, and low-density lipoprotein receptor) were regulated after n-3 PUFA supplementation, especially in dyslipidemic men. Conclusion: Gene expression analyses revealed several possible molecular pathways by which n-3 PUFAs lower the TG level and increase the HDL-C and low-density lipoprotein level, whereupon the regulation of PPARα appear to play a central role. Trial registration. ClinicalTrials.gov (ID: NCT01089231). eng
dc.description.sponsorship BMBF
dc.language.iso eng
dc.publisher London : BioMed Central Ltd.
dc.relation.ispartofseries Lipids in Health and Disease 11 (2012)
dc.rights CC BY 2.0
dc.rights.uri https://creativecommons.org/licenses/by/2.0/
dc.subject Dyslipidemia eng
dc.subject HNF eng
dc.subject Omega-3 fatty acids eng
dc.subject PPARα eng
dc.subject RXR eng
dc.subject TG lowering eng
dc.subject 2 acylglycerol o acyltransferase 2 eng
dc.subject 2 acylglycerol o acyltransferase 3 eng
dc.subject ABC transporter G5 eng
dc.subject acyltransferase eng
dc.subject apolipoprotein C2 eng
dc.subject cholesterol acyltransferase eng
dc.subject cholesterol acyltransferase 1 eng
dc.subject diacylglycerol acyltransferase 1 eng
dc.subject docosahexaenoic acid eng
dc.subject fish oil eng
dc.subject hepatic nuclear factor 1beta eng
dc.subject hepatic nuclear factor 6 eng
dc.subject high density lipoprotein cholesterol eng
dc.subject icosapentaenoic acid eng
dc.subject low density lipoprotein receptor eng
dc.subject membrane protein eng
dc.subject nuclear factor eng
dc.subject omega 3 fatty acid eng
dc.subject peroxisome proliferator activated receptor alpha eng
dc.subject phospholipid transfer protein eng
dc.subject retinoid X receptor alpha eng
dc.subject retinoid X receptor gamma eng
dc.subject triacylglycerol eng
dc.subject unclassified drug eng
dc.subject article eng
dc.subject blood cell eng
dc.subject blood sampling eng
dc.subject cholesterol metabolism eng
dc.subject clinical article eng
dc.subject controlled clinical trial eng
dc.subject controlled study eng
dc.subject diet supplementation eng
dc.subject dyslipidemia eng
dc.subject gene expression eng
dc.subject genome analysis eng
dc.subject human eng
dc.subject lipid blood level eng
dc.subject lipid metabolism eng
dc.subject lipogenesis eng
dc.subject male eng
dc.subject microarray analysis eng
dc.subject nucleotide sequence eng
dc.subject protein blood level eng
dc.subject real time polymerase chain reaction eng
dc.subject Adult eng
dc.subject Cholesterol, HDL eng
dc.subject Dietary Supplements eng
dc.subject Dyslipidemias eng
dc.subject Fatty Acids, Omega-3 eng
dc.subject Fish Oils eng
dc.subject Gene Expression eng
dc.subject Genome, Human eng
dc.subject Humans eng
dc.subject Lipid Metabolism eng
dc.subject Lipids eng
dc.subject Lipoproteins, LDL eng
dc.subject Male eng
dc.subject Microarray Analysis eng
dc.subject Middle Aged eng
dc.subject PPAR alpha eng
dc.subject Triglycerides eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Regulation of lipid metabolism-related gene expression in whole blood cells of normo- and dyslipidemic men after fish oil supplementation
dc.type article
dc.type Text
dc.relation.issn 1476-511X
dc.relation.doi http://dx.doi.org/10.1186/1476-511X-11-172
dc.bibliographicCitation.volume 11
dc.bibliographicCitation.firstPage 172
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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