Blue-native PAGE in plants: a tool in analysis of protein-protein interactions

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dc.identifier.uri http://dx.doi.org/10.15488/46
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/64
dc.contributor.author Eubel, Holger
dc.contributor.author Braun, Hans-Peter
dc.contributor.author Millar, A. Harvey
dc.date.accessioned 2015-10-02T15:52:04Z
dc.date.available 2015-10-02T15:52:04Z
dc.date.issued 2005
dc.identifier.citation Eubel, Holger; Braun, Hans-Peter; Millar, A. Harvey: Blue-native PAGE in plants: a tool in analysis of protein-protein interactions. In: Plant Methods 1 (2005). DOI: http://dx.doi.org/10.1186/1746-4811-1-11
dc.description.abstract Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylarnide gel electrophoresis system combining mild detergents and the dye Coornassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylarnide Gel-Electrophoresis (BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteornics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science. eng
dc.description.sponsorship DFG/Br1829-7/1
dc.description.sponsorship ARC Centre of Excellence Program
dc.language.iso eng
dc.publisher London : Biomed Central LTD
dc.relation.ispartofseries Plant Methods 1 (2005)
dc.rights CC BY 2.0 Unported
dc.rights.uri http://creativecommons.org/licenses/by/2.0
dc.subject Blue native PAGE eng
dc.subject Destabilization eng
dc.subject Plant proteomics eng
dc.subject Protein complex eng
dc.subject.ddc 580 | Pflanzen (Botanik)
dc.title Blue-native PAGE in plants: a tool in analysis of protein-protein interactions eng
dc.type article
dc.type Text
dc.relation.issn 1746-4811
dc.relation.doi 10.1186/1746-4811-1-11
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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