Blue-native PAGE in plants: a tool in analysis of protein-protein interactions

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Eubel, Holger; Braun, Hans-Peter; Millar, A. Harvey: Blue-native PAGE in plants: a tool in analysis of protein-protein interactions. In: Plant Methods 1 (2005). DOI: http://dx.doi.org/10.1186/1746-4811-1-11

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Sum total of downloads: 901




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Abstract: 
Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylarnide gel electrophoresis system combining mild detergents and the dye Coornassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylarnide Gel-Electrophoresis (BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteornics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science.
License of this version: CC BY 2.0 Unported
Document Type: article
Publishing status: publishedVersion
Issue Date: 2005
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
total perc.
1 image of flag of Germany Germany 755 83.80%
2 image of flag of United States United States 29 3.22%
3 image of flag of No geo information available No geo information available 14 1.55%
4 image of flag of France France 13 1.44%
5 image of flag of Russian Federation Russian Federation 11 1.22%
6 image of flag of China China 11 1.22%
7 image of flag of Netherlands Netherlands 10 1.11%
8 image of flag of Indonesia Indonesia 6 0.67%
9 image of flag of Ukraine Ukraine 4 0.44%
10 image of flag of Italy Italy 4 0.44%
    other countries 44 4.88%

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