Evaluation of the soft tissue biocompatibility of MgCa0.8 and surgical steel 316L in vivo: A comparative study in rabbits

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dc.identifier.uri http://dx.doi.org/10.15488/595
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/619
dc.contributor.author Erdmann, Nina
dc.contributor.author Bondarenko, Alexandr
dc.contributor.author Hewicker-Trautwein, Marion
dc.contributor.author Angrisani, Nina
dc.contributor.author Reifenrath, Janin
dc.contributor.author Lucas, Arne
dc.contributor.author Meyer-Lindenberg, Andrea
dc.date.accessioned 2016-10-31T13:37:18Z
dc.date.available 2016-10-31T13:37:18Z
dc.date.issued 2010
dc.identifier.citation Erdmann, N.; Bondarenko, A.; Hewicker-Trautwein, M.; Angrisani, N.; Reifenrath, J.et al.: Evaluation of the soft tissue biocompatibility of MgCa0.8 and surgical steel 316L in vivo: A comparative study in rabbits. In: BioMedical Engineering Online 9 (2010), 63. DOI: http://dx.doi.org/10.1186/1475-925X-9-63
dc.description.abstract Background: Recent studies have shown the potential suitability of magnesium alloys as biodegradable implants. The aim of the present study was to compare the soft tissue biocompatibility of MgCa0.8 and commonly used surgical steel in vivo.Methods: A biodegradable magnesium calcium alloy (MgCa0.8) and surgical steel (S316L), as a control, were investigated. Screws of identical geometrical conformation were implanted into the tibiae of 40 rabbits for a postoperative follow up of two, four, six and eight weeks. The tibialis cranialis muscle was in direct vicinity of the screw head and thus embedded in paraffin and histologically and immunohistochemically assessed. Haematoxylin and eosin staining was performed to identify macrophages, giant cells and heterophil granulocytes as well as the extent of tissue fibrosis and necrosis. Mouse anti-CD79α and rat anti-CD3 monoclonal primary antibodies were used for B- and T-lymphocyte detection. Evaluation of all sections was performed by applying a semi-quantitative score.Results: Clinically, both implant materials were tolerated well. Histology revealed that a layer of fibrous tissue had formed between implant and overlying muscle in MgCa0.8 and S316L, which was demarcated by a layer of synoviocyte-like cells at its interface to the implant. In MgCa0.8 implants cavities were detected within the fibrous tissue, which were surrounded by the same kind of cell type. The thickness of the fibrous layer and the amount of tissue necrosis and cellular infiltrations gradually decreased in S316L. In contrast, a decrease could only be noted in the first weeks of implantation in MgCa0.8, whereas parameters were increasing again at the end of the observation period. B-lymphocytes were found more often in MgCa0.8 indicating humoral immunity and the presence of soluble antigens. Conversely, S316L displayed a higher quantity of T-lymphocytes.Conclusions: Moderate inflammation was detected in both implant materials and resolved to a minimum during the first weeks indicating comparable biocompatibility for MgCa0.8 and S316L. Thus, the application of MgCa0.8 as biodegradable implant material seems conceivable. Since the inflammatory parameters were re-increasing at the end of the observation period in MgCa0.8 it is important to observe the development of inflammation over a longer time period in addition to the present study. eng
dc.description.sponsorship DFG/Collaborative Research Centre/599
dc.language.iso eng
dc.publisher London : BioMed Central Ltd.
dc.relation.ispartofseries BioMedical Engineering Online 9 (2010)
dc.rights CC BY 2.0
dc.rights.uri https://creativecommons.org/licenses/by/2.0/
dc.subject Anti-cd3 eng
dc.subject B lymphocytes eng
dc.subject Cell types eng
dc.subject Cellular infiltration eng
dc.subject Commonly used eng
dc.subject Comparative studies eng
dc.subject Fibrous tissue eng
dc.subject Follow up eng
dc.subject Geometrical conformation eng
dc.subject Giant cells eng
dc.subject Haematoxylin eng
dc.subject Humoral immunity eng
dc.subject Implant materials eng
dc.subject In-vivo eng
dc.subject Magnesium-calcium alloys eng
dc.subject Observation Period eng
dc.subject Screw head eng
dc.subject Semi-quantitative eng
dc.subject Soft tissue eng
dc.subject Synoviocytes eng
dc.subject Time-periods eng
dc.subject Tissue necrosis eng
dc.subject Antibodies eng
dc.subject Calcium alloys eng
dc.subject Chemical detection eng
dc.subject Magnesium eng
dc.subject Magnesium alloys eng
dc.subject Muscle eng
dc.subject Paraffin waxes eng
dc.subject Paraffins eng
dc.subject Pathology eng
dc.subject Screws eng
dc.subject Surgery eng
dc.subject Biocompatibility eng
dc.subject Oryctolagus cuniculus eng
dc.subject Rattus eng
dc.subject alloy eng
dc.subject biomaterial eng
dc.subject calcium eng
dc.subject magnesium eng
dc.subject steel eng
dc.subject animal eng
dc.subject article eng
dc.subject biodegradable implant eng
dc.subject bone screw eng
dc.subject chemically induced disorder eng
dc.subject chemistry eng
dc.subject comparative study eng
dc.subject drug effect eng
dc.subject female eng
dc.subject general surgery eng
dc.subject inflammation eng
dc.subject materials testing eng
dc.subject methodology eng
dc.subject pathology eng
dc.subject rabbit eng
dc.subject radiography eng
dc.subject skeletal muscle eng
dc.subject tibia eng
dc.subject Absorbable Implants eng
dc.subject Alloys eng
dc.subject Animals eng
dc.subject Biocompatible Materials eng
dc.subject Bone Screws eng
dc.subject Calcium eng
dc.subject Female eng
dc.subject General Surgery eng
dc.subject Inflammation eng
dc.subject Magnesium eng
dc.subject Materials Testing eng
dc.subject Muscle, Skeletal eng
dc.subject Rabbits eng
dc.subject Steel eng
dc.subject Tibia eng
dc.subject.ddc 600 | Technik ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Evaluation of the soft tissue biocompatibility of MgCa0.8 and surgical steel 316L in vivo: A comparative study in rabbits
dc.type article
dc.type Text
dc.relation.issn 1475-925X
dc.relation.doi http://dx.doi.org/10.1186/1475-925X-9-63
dc.bibliographicCitation.volume 9
dc.bibliographicCitation.firstPage 63
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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