Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells

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dc.identifier.uri http://dx.doi.org/10.15488/462
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/485
dc.contributor.author Lavrentieva, Antonina
dc.contributor.author Majore, Ingrida
dc.contributor.author Kasper, Cornelia
dc.contributor.author Hass, Ralf
dc.date.accessioned 2016-08-30T10:20:24Z
dc.date.available 2016-08-30T10:20:24Z
dc.date.issued 2010-06-16
dc.identifier.citation Lavrentieva, Antonina; Majore, Ingrida; Kasper, Cornelia; Hass, Ralf: Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells. In: Cell Communication and Signaling 8 (2010) , 18. DOI: http://dx.doi.org/10.1186/1478-811X-8-18
dc.description.abstract Following cultivation of distinct mesenchymal stem cell (MSC) populations derived from human umbilical cord under hypoxic conditions (between 1.5% to 5% oxygen (O-2)) revealed a 2- to 3-fold reduced oxygen consumption rate as compared to the same cultures at normoxic oxygen levels (21% O-2). A simultaneous measurement of dissolved oxygen within the culture media from 4 different MSC donors ranged from 15 mu mol/L at 1.5% O-2 to 196 mu mol/L at normoxic 21% O-2. The proliferative capacity of the different hypoxic MSC populations was elevated as compared to the normoxic culture. This effect was paralleled by a significantly reduced cell damage or cell death under hypoxic conditions as evaluated by the cellular release of LDH whereby the measurement of caspase 3/7 activity revealed little if any differences in apoptotic cell death between the various cultures. The MSC culture under hypoxic conditions was associated with the induction of hypoxia-inducing factor-alpha (HIF-1 alpha) and an elevated expression of energy metabolism-associated genes including GLUT-1, LDH and PDK1. Concomitantly, a significantly enhanced glucose consumption and a corresponding lactate production could be observed in the hypoxic MSC cultures suggesting an altered metabolism of these human stem cells within the hypoxic environment. eng
dc.description.sponsorship DFG/EXC/REBIRTH
dc.language.iso eng
dc.publisher London : Biomed Central Ltd
dc.relation.ispartofseries Cell Communication and Signaling 8 (2010)
dc.rights CC BY 2.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/2.0/
dc.subject adipose-tissue eng
dc.subject bone-marrow eng
dc.subject oxygen concentration eng
dc.subject reactive oxygen eng
dc.subject metabolism eng
dc.subject growth eng
dc.subject consumption eng
dc.subject responses eng
dc.subject damage eng
dc.subject dna eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.title Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells
dc.type article
dc.type Text
dc.relation.issn 1478-811X
dc.relation.doi http://dx.doi.org/10.1186/1478-811X-8-18
dc.bibliographicCitation.volume 8
dc.bibliographicCitation.firstPage 18
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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