Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells

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Lavrentieva, Antonina; Majore, Ingrida; Kasper, Cornelia; Hass, Ralf: Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells. In: Cell Communication and Signaling 8 (2010) , 18. DOI: http://dx.doi.org/10.1186/1478-811X-8-18

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Following cultivation of distinct mesenchymal stem cell (MSC) populations derived from human umbilical cord under hypoxic conditions (between 1.5% to 5% oxygen (O-2)) revealed a 2- to 3-fold reduced oxygen consumption rate as compared to the same cultures at normoxic oxygen levels (21% O-2). A simultaneous measurement of dissolved oxygen within the culture media from 4 different MSC donors ranged from 15 mu mol/L at 1.5% O-2 to 196 mu mol/L at normoxic 21% O-2. The proliferative capacity of the different hypoxic MSC populations was elevated as compared to the normoxic culture. This effect was paralleled by a significantly reduced cell damage or cell death under hypoxic conditions as evaluated by the cellular release of LDH whereby the measurement of caspase 3/7 activity revealed little if any differences in apoptotic cell death between the various cultures. The MSC culture under hypoxic conditions was associated with the induction of hypoxia-inducing factor-alpha (HIF-1 alpha) and an elevated expression of energy metabolism-associated genes including GLUT-1, LDH and PDK1. Concomitantly, a significantly enhanced glucose consumption and a corresponding lactate production could be observed in the hypoxic MSC cultures suggesting an altered metabolism of these human stem cells within the hypoxic environment.
License of this version: CC BY 2.0 Unported
Document Type: article
Publishing status: publishedVersion
Issue Date: 2010-06-16
Appears in Collections:Naturwissenschaftliche Fakultät

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