Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells

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Lavrentieva, Antonina; Majore, Ingrida; Kasper, Cornelia; Hass, Ralf: Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells. In: Cell Communication and Signaling 8 (2010) , 18. DOI: http://dx.doi.org/10.1186/1478-811X-8-18

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Abstract: 
Following cultivation of distinct mesenchymal stem cell (MSC) populations derived from human umbilical cord under hypoxic conditions (between 1.5% to 5% oxygen (O-2)) revealed a 2- to 3-fold reduced oxygen consumption rate as compared to the same cultures at normoxic oxygen levels (21% O-2). A simultaneous measurement of dissolved oxygen within the culture media from 4 different MSC donors ranged from 15 mu mol/L at 1.5% O-2 to 196 mu mol/L at normoxic 21% O-2. The proliferative capacity of the different hypoxic MSC populations was elevated as compared to the normoxic culture. This effect was paralleled by a significantly reduced cell damage or cell death under hypoxic conditions as evaluated by the cellular release of LDH whereby the measurement of caspase 3/7 activity revealed little if any differences in apoptotic cell death between the various cultures. The MSC culture under hypoxic conditions was associated with the induction of hypoxia-inducing factor-alpha (HIF-1 alpha) and an elevated expression of energy metabolism-associated genes including GLUT-1, LDH and PDK1. Concomitantly, a significantly enhanced glucose consumption and a corresponding lactate production could be observed in the hypoxic MSC cultures suggesting an altered metabolism of these human stem cells within the hypoxic environment.
License of this version: CC BY 2.0 Unported
Document Type: article
Publishing status: publishedVersion
Issue Date: 2010-06-16
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
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1 image of flag of Germany Germany 173 90.10%
2 image of flag of United States United States 7 3.65%
3 image of flag of China China 5 2.60%
4 image of flag of Latvia Latvia 1 0.52%
5 image of flag of India India 1 0.52%
6 image of flag of Indonesia Indonesia 1 0.52%
7 image of flag of Hungary Hungary 1 0.52%
8 image of flag of Switzerland Switzerland 1 0.52%
9 image of flag of Canada Canada 1 0.52%
10 image of flag of Brazil Brazil 1 0.52%

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