Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein

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dc.identifier.uri http://dx.doi.org/10.15488/4584
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/4626
dc.contributor.author Petersen, Svea
dc.contributor.author Soller, Jan T.
dc.contributor.author Wagner, Siegfried
dc.contributor.author Richter, Andreas
dc.contributor.author Bullerdiek, Jörn
dc.contributor.author Nolte, Ingo
dc.contributor.author Barcikowski, Stephan
dc.contributor.author Murua Escobar, Hugo
dc.date.accessioned 2019-03-27T11:25:35Z
dc.date.available 2019-03-27T11:25:35Z
dc.date.issued 2009
dc.identifier.citation Petersen, S.; Soller, J.T.; Wagner, S.; Richter, A.; Bullerdiek, J. et al.: Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein. In: Journal of Nanobiotechnology 7 (2009), 6. DOI: https://doi.org/10.1186/1477-3155-7-6
dc.description.abstract Ultrashort pulsed laser ablation in liquids represents a powerful tool for the generation of pure gold nanoparticles (AuNPs) avoiding chemical precursors and thereby making them especially interesting for biomedical applications. However, because of their electron accepting properties, laser-generated AuNPs might affect biochemical properties of biomolecules, which often adsorb onto the nanoparticles. We investigated possible effects of such laser-generated AuNPs on biological functionality of DNA molecules. We tested four differently sized and positively charged AuNPs by incubating them with recombinant eGFP-C1-HMGB1 DNA expression plasmids that code for eGFP fusion proteins and contain the canine architectural transcription factor HMGB1. We were able to show that successfully transfected mammalian cells are still able to synthesize and process the fusion proteins. Our observations revealed that incubation of AuNP with the plasmid DNA encoding the recombinant canine HMGB1 neither prevented the mediated uptake of the vector through the plasma membrane in presence of a transfection reagent nor had any effect on the transport of the synthesized fusion proteins to the nuclei. Biological activity of the recombinant GFP-HMGB1 fusion protein appears to have not been affected either, as a strong characteristic protein accumulation in the nucleus could be observed. We also discovered that transfection efficiencies depend on the size of AuNP. In conclusion, our data indicate that laser-generated AuNPs present a good alternative to chemically synthesized nanoparticles for use in biomedical applications. eng
dc.language.iso eng
dc.publisher London : Biomed Central
dc.relation.ispartofseries Journal of Nanobiotechnology 7 (2009)
dc.rights CC BY 2.0
dc.rights.uri https://creativecommons.org/licenses/by/2.0/
dc.subject Biochemical properties eng
dc.subject Biological activities eng
dc.subject Biomedical applications eng
dc.subject Chemical precursors eng
dc.subject Co-transfections eng
dc.subject DNA expressions eng
dc.subject DNA molecules eng
dc.subject Electron-accepting eng
dc.subject Fusion proteins eng
dc.subject Gold nanoparticle eng
dc.subject Gold Nanoparticles eng
dc.subject Mammalian cells eng
dc.subject Plasma membranes eng
dc.subject Plasmid DNA eng
dc.subject Positively charged eng
dc.subject Protein accumulation eng
dc.subject Transfection efficiency eng
dc.subject Transfection reagents eng
dc.subject Ultrashort-pulsed laser eng
dc.subject Bioactivity eng
dc.subject Cell membranes eng
dc.subject DNA eng
dc.subject Genes eng
dc.subject Laser ablation eng
dc.subject Lasers eng
dc.subject Mammals eng
dc.subject Molecular biology eng
dc.subject Nanoparticles eng
dc.subject Pulsed laser applications eng
dc.subject Transcription factors eng
dc.subject Nucleic acids eng
dc.subject enhanced green fluorescent protein eng
dc.subject gold nanoparticle eng
dc.subject high mobility group B1 protein eng
dc.subject hybrid protein eng
dc.subject plasmid DNA eng
dc.subject plasmid vector eng
dc.subject recombinant protein eng
dc.subject animal cell eng
dc.subject article eng
dc.subject cell nucleus eng
dc.subject controlled study eng
dc.subject gene expression eng
dc.subject genetic transfection eng
dc.subject laser eng
dc.subject nanobiotechnology eng
dc.subject nonhuman eng
dc.subject particle size eng
dc.subject protein localization eng
dc.subject protein transport eng
dc.subject recombinant plasmid eng
dc.subject Mammalia eng
dc.subject.ddc 540 | Chemie ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein
dc.type article
dc.type Text
dc.relation.issn 1477-3155
dc.relation.doi https://doi.org/10.1186/1477-3155-7-6
dc.bibliographicCitation.volume 7
dc.bibliographicCitation.firstPage 6
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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    Frei zugängliche Publikationen aus An-Instituten der Leibniz Universität Hannover

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