Strobilurin biosynthesis in Basidiomycete fungi

Show simple item record

dc.identifier.uri http://dx.doi.org/10.15488/4227
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/4261
dc.contributor.author Nofiani, Risa
dc.contributor.author De Mattos-Shipley, Kate M.J.
dc.contributor.author Lebe, Karen E.
dc.contributor.author Han, Li-Chen
dc.contributor.author Iqbal, Zafar
dc.contributor.author Bailey, Andrew M.
dc.contributor.author Willis, Christine L.
dc.contributor.author Simpson, Thomas J.
dc.contributor.author Cox, Russell J.
dc.date.accessioned 2018-12-19T11:04:43Z
dc.date.available 2018-12-19T11:04:43Z
dc.date.issued 2018
dc.identifier.citation Nofiani, R.; de Mattos-Shipley, K.; Lebe, K.E.; Han, L.-C.; Iqbal, Z. et al.: Strobilurin biosynthesis in Basidiomycete fungi. In: Nature Communications 9 (2018), Nr. 1, 3940. DOI: https://doi.org/10.1038/s41467-018-06202-4
dc.description.abstract Strobilurins from fungi are the inspiration for the creation of the β-methoxyacrylate class of agricultural fungicides. However, molecular details of the biosynthesis of strobilurins have remained cryptic. Here we report the sequence of genomes of two fungi that produce strobilurins and show that each contains a biosynthetic gene cluster, which encodes a highly reducing polyketide synthase with very unusual C-terminal hydrolase and methyltransferase domains. Expression of stpks1 in Aspergillus oryzae leads to the production of prestrobilurin A when the fermentation is supplemented with a benzoyl coenzyme A (CoA) analogue. This enables the discovery of a previously unobserved route to benzoyl CoA. Reconstruction of the gene cluster in A. oryzae leads to the formation of prestrobilurin A, and addition of the gene str9 encoding an FAD-dependent oxygenase leads to the key oxidative rearrangement responsible for the creation of the β-methoxyacrylate toxophore. Finally, two methyltransferases are required to complete the synthesis. eng
dc.language.iso eng
dc.publisher London : Nature Publishing Group
dc.relation.ispartofseries Nature Communications 9 (2018), Nr. 1
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Aspergillus oryzae eng
dc.subject Fungi eng
dc.subject Toxophora eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.subject.ddc 540 | Chemie ger
dc.title Strobilurin biosynthesis in Basidiomycete fungi eng
dc.type article
dc.type Text
dc.relation.issn 20411723
dc.relation.doi https://doi.org/10.1038/s41467-018-06202-4
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 9
dc.bibliographicCitation.firstPage 3940
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


Files in this item

This item appears in the following Collection(s):

Show simple item record

 

Search the repository


Browse

My Account

Usage Statistics