dc.identifier.uri |
http://dx.doi.org/10.15488/4165 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/4199 |
|
dc.contributor.author |
Vásquez-Rivera, Andrés
|
|
dc.contributor.author |
Oldenhof, Harriette
|
|
dc.contributor.author |
Dipresa, Daniele
|
|
dc.contributor.author |
Goecke, Tobias
|
|
dc.contributor.author |
Kouvaka, Artemis
|
|
dc.contributor.author |
Will, Fabian
|
|
dc.contributor.author |
Haverich, Axel
|
|
dc.contributor.author |
Korossis, Sotirios
|
|
dc.contributor.author |
Hilfiker, Andres
|
|
dc.contributor.author |
Wolkers, Willem F.
|
|
dc.date.accessioned |
2018-12-14T13:58:55Z |
|
dc.date.available |
2018-12-14T13:58:55Z |
|
dc.date.issued |
2018 |
|
dc.identifier.citation |
Vásquez-Rivera, A.; Oldenhof, H.; Dipresa, D.; Goecke, T.; Kouvaka, A. et al.: Use of sucrose to diminish pore formation in freeze-dried heart valves. In: Scientific Reports 8 (2018), Nr. 1, 12982. DOI: https://doi.org/10.1038/s41598-018-31388-4 |
|
dc.description.abstract |
Freeze-dried storage of decellularized heart valves provides easy storage and transport for clinical use. Freeze-drying without protectants, however, results in a disrupted histoarchitecture after rehydration. In this study, heart valves were incubated in solutions of various sucrose concentrations and subsequently freeze-dried. Porosity of rehydrated valves was determined from histological images. In the absence of sucrose, freeze-dried valves were shown to have pores after rehydration in the cusp, artery and muscle sections. Use of sucrose reduced pore formation in a dose-dependent manner, and pretreatment of the valves in a 40% (w/v) sucrose solution prior to freeze-drying was found to be sufficient to completely diminish pore formation. The presence of pores in freeze-dried valves was found to coincide with altered biomechanical characteristics, whereas biomechanical parameters of valves freeze-dried with enough sucrose were not significantly different from those of valves not exposed to freeze-drying. Multiphoton imaging, Fourier transform infrared spectroscopy, and differential scanning calorimetry studies revealed that matrix proteins (i.e. collagen and elastin) were not affected by freeze-drying. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
London : Nature Publishing Group |
|
dc.relation.ispartofseries |
Scientific Reports 8 (2018), Nr. 1 |
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dc.rights |
CC BY 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
freeze-drying |
eng |
dc.subject |
heart valves |
eng |
dc.subject |
porosity |
eng |
dc.subject.ddc |
600 | Technik
|
ger |
dc.title |
Use of sucrose to diminish pore formation in freeze-dried heart valves |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.issn |
20452322 |
|
dc.relation.doi |
https://doi.org/10.1038/s41598-018-31388-4 |
|
dc.bibliographicCitation.issue |
1 |
|
dc.bibliographicCitation.volume |
8 |
|
dc.bibliographicCitation.firstPage |
12982 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|