Use of sucrose to diminish pore formation in freeze-dried heart valves

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Vásquez-Rivera, A.; Oldenhof, H.; Dipresa, D.; Goecke, T.; Kouvaka, A. et al.: Use of sucrose to diminish pore formation in freeze-dried heart valves. In: Scientific Reports 8 (2018), Nr. 1, 12982. DOI: https://doi.org/10.1038/s41598-018-31388-4

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Abstract: 
Freeze-dried storage of decellularized heart valves provides easy storage and transport for clinical use. Freeze-drying without protectants, however, results in a disrupted histoarchitecture after rehydration. In this study, heart valves were incubated in solutions of various sucrose concentrations and subsequently freeze-dried. Porosity of rehydrated valves was determined from histological images. In the absence of sucrose, freeze-dried valves were shown to have pores after rehydration in the cusp, artery and muscle sections. Use of sucrose reduced pore formation in a dose-dependent manner, and pretreatment of the valves in a 40% (w/v) sucrose solution prior to freeze-drying was found to be sufficient to completely diminish pore formation. The presence of pores in freeze-dried valves was found to coincide with altered biomechanical characteristics, whereas biomechanical parameters of valves freeze-dried with enough sucrose were not significantly different from those of valves not exposed to freeze-drying. Multiphoton imaging, Fourier transform infrared spectroscopy, and differential scanning calorimetry studies revealed that matrix proteins (i.e. collagen and elastin) were not affected by freeze-drying.
License of this version: CC BY 4.0 Unported
Document Type: article
Publishing status: publishedVersion
Issue Date: 2018
Appears in Collections:Fakultät für Maschinenbau

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pos. country downloads
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1 image of flag of Germany Germany 70 82.35%
2 image of flag of United States United States 8 9.41%
3 image of flag of Sweden Sweden 2 2.35%
4 image of flag of No geo information available No geo information available 1 1.18%
5 image of flag of Russian Federation Russian Federation 1 1.18%
6 image of flag of Kazakhstan Kazakhstan 1 1.18%
7 image of flag of China China 1 1.18%
8 image of flag of Brazil Brazil 1 1.18%

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