On Chip Protein Pre-Concentration for Enhancing the Sensitivity of Porous Silicon Biosensors

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dc.identifier.uri http://dx.doi.org/10.15488/4118
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/4152
dc.contributor.author Arshavsky-Graham, Sofia ger
dc.contributor.author Massad-Ivanir, Naama ger
dc.contributor.author Paratore, Federico ger
dc.contributor.author Scheper, Thomas ger
dc.contributor.author Bercovici, Moran ger
dc.contributor.author Segal, Ester ger
dc.date.accessioned 2018-12-06T14:22:16Z
dc.date.available 2018-12-06T14:22:16Z
dc.date.issued 2017
dc.identifier.citation Arshavsky-Graham, S. et al.: On Chip Protein Pre-Concentration for Enhancing the Sensitivity of Porous Silicon Biosensors. In: ACS Sensors 2 (2017), S. 1767-1773. DOI: https://doi.org/10.1021/acssensors.7b00692 ger
dc.description.abstract Porous silicon (PSi) nanomaterials have been widely studied as label-free optical biosensors for protein detection. However, these biosensors’ performance, specifically in terms of their sensitivity (which is typically in the micromolar range), is insufficient for many applications. Herein, we present a proof-of-concept application of the electrokinetic isotachophoresis (ITP) technique for real-time preconcentration of a target protein on a PSi biosensor. With ITP, a highly concentrated target zone is delivered to the sensing area, where the protein target is captured by immobilized aptamers. The detection of the binding events is conducted in a label-free manner by reflective interferometric Fourier transformation spectroscopy (RIFTS). Up to 1000-fold enhancement in local concentration of the protein target and the biosensor’s sensitivity are achieved, with a measured limit of detection of 7.5 nM. Furthermore, the assay is successfully performed in complex media, such as bacteria lysate samples, while the selectivity of the biosensor is retained. The presented assay could be further utilized for other protein targets, and to promote the development of clinically useful PSi biosensors. ger
dc.language.iso eng ger
dc.publisher Washington D.C. : American Chemical Society
dc.relation.ispartofseries ACS Sensors 2 (2017) ger
dc.rights ACS AuthorChoice License ger
dc.rights.uri https://pubs.acs.org/page/policy/authorchoice_termsofuse.html
dc.subject aptamer eng
dc.subject isotachophoresis eng
dc.subject label-free eng
dc.subject optical biosensor eng
dc.subject porous silicon eng
dc.subject.ddc 540 | Chemie ger
dc.title On Chip Protein Pre-Concentration for Enhancing the Sensitivity of Porous Silicon Biosensors ger
dc.type article ger
dc.type Text ger
dc.relation.doi 10.1021/acssensors.7b00692
dc.description.version publishedVersion ger


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