Development and characterisation of a new fluorescence sensor for online monitoring of bioprocesses

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dc.identifier.uri http://dx.doi.org/10.15488/3781
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/3815
dc.contributor.author König, Jan C.
dc.contributor.author Steinwedel, Tobias
dc.contributor.author Solle, Dörte
dc.contributor.author Lindner, Patrick
dc.contributor.author de Vries, Ingo
dc.contributor.author Hentrop, Thorleif
dc.contributor.author Findeis, Michael
dc.contributor.author John, Gernot T.
dc.contributor.author Scheper, Thomas
dc.contributor.author Beutel, Sascha
dc.date.accessioned 2018-10-10T08:42:36Z
dc.date.available 2018-10-10T08:42:36Z
dc.date.issued 2018
dc.identifier.citation König, J.C.; Steinwedel, T.; Solle, D.; Lindner, P.; De Vries, I. et al.: Development and characterisation of a new fluorescence sensor for online monitoring of bioprocesses. In: Journal of Sensors and Sensor Systems 7 (2018), Nr. 2, S. 461-467. DOI: https://doi.org/10.5194/jsss-7-461-2018
dc.description.abstract Fluorescence spectroscopy is a highly sensitive and non-invasive technique for the identification of characteristic process states and for the online monitoring of substrate and product concentrations. Nevertheless, fluorescence sensors are mainly used in academic studies and are not well implemented for monitoring of industrial production processes. In this work, we present a newly developed robust online fluorescence sensor that facilitates the analysis of fluorescence measurements. The set-up of the sensor was miniaturised and realised without any moveable part to be robust enough for application in technical environments. It was constructed to measure only the three most important biologic fluorophores (tryptophan, NADH and FAD/FMN), resulting in a significant data reduction compared to conventional a 2-D fluorescence spectrometer. The sensor performance was evaluated by calibration curves and selectivity tests. The measuring ranges were determined as 0.5-50 μmolL-1 for NADH and 0.0025-7.5 μmolL-1 for BSA and riboflavin. Online monitoring of batch cultivations of wild-type Escherichia coli K1 in a 10 L bioreactor scale were performed. The data sets were analysed using principal component analysis and partial least square regression. The recorded fluorescence data were successfully used to predict the biomass of an independent cultivation (RMSEP 4.6 %). eng
dc.language.iso eng
dc.publisher Göttingen : Copernicus GmbH
dc.relation.ispartofseries Journal of Sensors and Sensor Systems 7 (2018), Nr. 2
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject fluorescence spectroscopy eng
dc.subject substrate concentrations eng
dc.subject measurement eng
dc.subject.ddc 600 | Technik ger
dc.subject.ddc 540 | Chemie ger
dc.title Development and characterisation of a new fluorescence sensor for online monitoring of bioprocesses eng
dc.type article
dc.type Text
dc.relation.issn 21948771
dc.relation.doi https://doi.org/10.5194/jsss-7-461-2018
dc.bibliographicCitation.issue 2
dc.bibliographicCitation.volume 7
dc.bibliographicCitation.firstPage 461
dc.bibliographicCitation.lastPage 467
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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