Development and characterisation of a new fluorescence sensor for online monitoring of bioprocesses

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König, J.C.; Steinwedel, T.; Solle, D.; Lindner, P.; De Vries, I. et al.: Development and characterisation of a new fluorescence sensor for online monitoring of bioprocesses. In: Journal of Sensors and Sensor Systems 7 (2018), Nr. 2, S. 461-467. DOI:

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Sum total of downloads: 193

Fluorescence spectroscopy is a highly sensitive and non-invasive technique for the identification of characteristic process states and for the online monitoring of substrate and product concentrations. Nevertheless, fluorescence sensors are mainly used in academic studies and are not well implemented for monitoring of industrial production processes. In this work, we present a newly developed robust online fluorescence sensor that facilitates the analysis of fluorescence measurements. The set-up of the sensor was miniaturised and realised without any moveable part to be robust enough for application in technical environments. It was constructed to measure only the three most important biologic fluorophores (tryptophan, NADH and FAD/FMN), resulting in a significant data reduction compared to conventional a 2-D fluorescence spectrometer. The sensor performance was evaluated by calibration curves and selectivity tests. The measuring ranges were determined as 0.5-50 μmolL-1 for NADH and 0.0025-7.5 μmolL-1 for BSA and riboflavin. Online monitoring of batch cultivations of wild-type Escherichia coli K1 in a 10 L bioreactor scale were performed. The data sets were analysed using principal component analysis and partial least square regression. The recorded fluorescence data were successfully used to predict the biomass of an independent cultivation (RMSEP 4.6 %).
License of this version: CC BY 4.0 Unported
Document Type: article
Publishing status: publishedVersion
Issue Date: 2018
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
total perc.
1 image of flag of Germany Germany 101 52.33%
2 image of flag of United States United States 32 16.58%
3 image of flag of Ireland Ireland 8 4.15%
4 image of flag of Spain Spain 7 3.63%
5 image of flag of China China 6 3.11%
6 image of flag of India India 4 2.07%
7 image of flag of United Kingdom United Kingdom 4 2.07%
8 image of flag of Canada Canada 4 2.07%
9 image of flag of Brazil Brazil 4 2.07%
10 image of flag of Austria Austria 3 1.55%
    other countries 20 10.36%

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