Modrejewski, J.; Walter, J.-G.; Kretschmer, I.; Kemal, E.; Green, M. et al.: Aptamer-modified polymer nanoparticles for targeted drug delivery. In: BioNanoMaterials 17 (2016), Nr. Februar, S. 43-51. DOI:
https://doi.org/10.1515/bnm-2015-0027
Abstract: |
The purpose of this study was to develop a model system for targeted drug delivery. This system should enable targeted drug release at a certain tissue in the body. In conventional drug delivery systems, drugs are often delivered unspecifically resulting in unwarranted adverse effects. To circumvent this problem, there is an increasing demand for the development of intelligent drug delivery systems allowing a tissue-specific mode of delivery. Within this study, nanoparticles consisting of two biocompatible polymers are used. Because of their small size, nanoparticles are well-suited for effective drug delivery. The small size affects their movement through cell and tissue barriers. Their cellular uptake is easier when compared to larger drug delivery systems. Paclitaxel was encapsulated into the nanoparticles as a model drug, and to achieve specific targeting an aptamer directed against lung cancer cells was coupled to the nanoparticles surface. Nanoparticles were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), and nanotracking analysis (NTA). Also their surface charge was characterized from ζ-potential measurements. Their preparation was optimized and subsequently specificity of drug-loaded and aptamer-functionalized nanoparticles was investigated using lung cancer cells. © 2016 by De Gruyter.
|
License of this version: |
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich. |
Publication type: |
Article |
Publishing status: |
publishedVersion |
Publication date: |
2016 |
Keywords english: |
aptamer, HOOC-PEG-PCL, mPEG-PCL, nanoparticles, aptamer, copolymer, nanocarrier, paclitaxel, antineoplastic activity, Article, cancer cell, controlled study, drug delivery system, drug dosage form comparison, drug release, drug solubility, female, high performance liquid chromatography, human, human cell, hydrodynamics, in vitro study, infrared spectroscopy, lung cancer, nanoencapsulation, particle size, photon correlation spectroscopy, polymerization, priority journal, process optimization, surface charge, transmission electron microscopy, zeta potential
|
DDC: |
610 | Medizin, Gesundheit
|