In vitro evaluation of PCL and P(3HB) as coating materials for selective laser melted porous titanium implants

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dc.identifier.uri Grau, Michael Matena, Julia Teske, Michael Petersen, Svea Aliuos, Pooyan Roland, Laura Grabow, Niels Escobar, Hugo Murua Gellrich, Nils-Claudius Haferkamp, Heinz Nolte, Ingo 2018-01-19T10:57:53Z 2018-01-19T10:57:53Z 2017
dc.identifier.citation Grau, M.; Matena, J.; Teske, M.; Petersen, S.; Aliuos, P. et al.: In vitro evaluation of PCL and P(3HB) as coating materials for selective laser melted porous titanium implants. In: Materials 10 (2017), Nr. 12, 1344. DOI:
dc.description.abstract Titanium is widely used as a bone implant material due to its biocompatibility and high resilience. Since its Young's modulus differs from bone tissue, the resulting "stress shielding" could lead to scaffold loosening. However, by using a scaffold-shaped geometry, the Young's modulus can be adjusted. Also, a porous geometry enables vascularisation and bone ingrowth inside the implant itself. Additionally, growth factors can improve these effects. In order to create a deposit and release system for these factors, the titanium scaffolds could be coated with degradable polymers. Therefore, in the present study, synthetic poly-"-caprolactone (PCL) and the biopolymer poly(3-hydroxybutyrate) (P(3HB)) were tested for coating efficiency, cell adhesion, and biocompatibility to find a suitable coating material. The underlying scaffold was created from titanium by Selective Laser Melting (SLM) and coated with PCL or P(3HB) via dip coating. To test the biocompatibility, Live Cell Imaging (LCI) as well as vitality and proliferation assays were performed. In addition, cell adhesion forces were detected via Single Cell Force Spectroscopy, while the coating efficiency was observed using environmental scanning electron microscopy (ESEM) and energy-dispersive X-ray (EDX) analyses. Regarding the coating efficiency, PCL showed higher values in comparison to P(3HB). Vitality assays revealed decent vitality values for both polymers, while values for PCL were significantly lower than those for blank titanium. No significant differences could be observed between PCL and P(3HB) in proliferation and cell adhesion studies. Although LCI observations revealed decreasing values in cell number and populated area over time on both polymer-coated scaffolds, these outcomes could be explained by the possibility of coating diluent residues accumulating in the culture medium. Overall, both polymers fulfill the requirements regarding biocompatibility. Nonetheless, since only PCL coating ensured the maintenance of the porous implant structure, it is preferable to be used as a coating material for creating a deposit and release system for growth factors. eng
dc.language.iso eng
dc.publisher Basel : MDPI AG
dc.relation.ispartofseries Materials 10 (2017), Nr. 12
dc.rights CC BY 4.0 Unported
dc.subject Osteoblast eng
dc.subject Poly(3-hydroxybutyrate) eng
dc.subject Polycaprolactone eng
dc.subject Titanium scaffold eng
dc.subject Biocompatibility eng
dc.subject Bone eng
dc.subject Cell adhesion eng
dc.subject Cells eng
dc.subject Coatings eng
dc.subject Cytology eng
dc.subject Deposits eng
dc.subject Elastic moduli eng
dc.subject Metal implants eng
dc.subject Osteoblasts eng
dc.subject Plastic coatings eng
dc.subject Plating eng
dc.subject Polycaprolactone eng
dc.subject Polymeric implants eng
dc.subject Polymers eng
dc.subject Scanning electron microscopy eng
dc.subject Titanium eng
dc.subject Bone implant material eng
dc.subject Energy dispersive x-ray eng
dc.subject Environmental scanning electron microscopies (ESEM) eng
dc.subject In-vitro evaluation eng
dc.subject Poly-3-hydroxybutyrate eng
dc.subject Porous titanium implants eng
dc.subject Selective laser melting eng
dc.subject Titanium scaffolds eng
dc.subject Scaffolds (biology) eng
dc.subject.ddc 620 | Ingenieurwissenschaften und Maschinenbau ger
dc.title In vitro evaluation of PCL and P(3HB) as coating materials for selective laser melted porous titanium implants
dc.type Article
dc.type Text
dc.relation.issn 19961944
dc.bibliographicCitation.issue 12
dc.bibliographicCitation.volume 10
dc.bibliographicCitation.firstPage 1344
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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