A comparison of cell wall disruption techniques for the isolation of intracellular metabolites from Pleurotus and Lepista sp.

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dc.identifier.uri http://dx.doi.org/10.15488/2373
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/2399
dc.contributor.author Taskova, Rilka M.
dc.contributor.author Zorn, Holger
dc.contributor.author Krings, Ulrich
dc.contributor.author Bouws, Henning
dc.contributor.author Berger, Ralf Günter
dc.date.accessioned 2017-11-24T07:40:27Z
dc.date.available 2017-11-24T07:40:27Z
dc.date.issued 2006
dc.identifier.citation Taskova, R.M.; Zorn, H.; Krings, U.; Bouws, H.; Berger, R.G.: A comparison of cell wall disruption techniques for the isolation of intracellular metabolites from Pleurotus and Lepista sp.. In: Zeitschrift für Naturforschung - Section C Journal of Biosciences 61 (2006), Nr. 5-6, S. 347-350. DOI: https://doi.org/10.1515/znc-2006-5-608
dc.description.abstract Different techniques were compared for their effectiveness in the disruption of the rigid cell walls of Basidiomycetes, Grinding under liquid nitrogen, stirred glass bead milling and enzymatic cell lysis were applied to the mycelia of Pleurotus sapidus and Lepista irina grown submerged. Each of the disruption procedures was evaluated by testing the quantity and quality of released intracellular metabolites: DNA, RNA, enzymes, and secondary metabolites. The most suitable method for nucleic acid isolation was grinding under liquid nitrogen, while bead mill homogenization was the superior technique for isolation of active enzymes. A new effective method is proposed for isolation of secondary metabolites with the aid of bead milling of fungal mycelia. © 2006 Verlag der Zeitschrift für Naturforschung. eng
dc.language.iso eng
dc.publisher Berlin : De Gruyter
dc.relation.ispartofseries Zeitschrift für Naturforschung - Section C Journal of Biosciences 61 (2006), Nr. 5-6
dc.rights CC BY-NC-ND 3.0
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject Basidiomycetes eng
dc.subject Cell Disruption eng
dc.subject catalase eng
dc.subject fungal protein eng
dc.subject glucose 6 phosphate dehydrogenase eng
dc.subject Agaricales eng
dc.subject article eng
dc.subject cell wall eng
dc.subject chemistry eng
dc.subject isolation and purification eng
dc.subject metabolism eng
dc.subject Pleurotus eng
dc.subject spectrophotometry eng
dc.subject ultrastructure eng
dc.subject Agaricales eng
dc.subject Catalase eng
dc.subject Cell Wall eng
dc.subject Fungal Proteins eng
dc.subject Glucosephosphate Dehydrogenase eng
dc.subject Pleurotus eng
dc.subject Spectrophotometry eng
dc.subject Basidiomycota eng
dc.subject Lepista eng
dc.subject Lepista irina eng
dc.subject Pleurotus eng
dc.subject Pleurotus sapidus eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title A comparison of cell wall disruption techniques for the isolation of intracellular metabolites from Pleurotus and Lepista sp.
dc.type article
dc.type Text
dc.relation.essn 1865-7125
dc.relation.issn 0939-5075
dc.relation.doi https://doi.org/10.1515/znc-2006-5-608
dc.bibliographicCitation.issue 5-6
dc.bibliographicCitation.volume 61
dc.bibliographicCitation.firstPage 347
dc.bibliographicCitation.lastPage 350
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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