A comparison of cell wall disruption techniques for the isolation of intracellular metabolites from Pleurotus and Lepista sp.

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Taskova, R.M.; Zorn, H.; Krings, U.; Bouws, H.; Berger, R.G.: A comparison of cell wall disruption techniques for the isolation of intracellular metabolites from Pleurotus and Lepista sp.. In: Zeitschrift für Naturforschung - Section C Journal of Biosciences 61 (2006), Nr. 5-6, S. 347-350. DOI: https://doi.org/10.1515/znc-2006-5-608

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Abstract: 
Different techniques were compared for their effectiveness in the disruption of the rigid cell walls of Basidiomycetes, Grinding under liquid nitrogen, stirred glass bead milling and enzymatic cell lysis were applied to the mycelia of Pleurotus sapidus and Lepista irina grown submerged. Each of the disruption procedures was evaluated by testing the quantity and quality of released intracellular metabolites: DNA, RNA, enzymes, and secondary metabolites. The most suitable method for nucleic acid isolation was grinding under liquid nitrogen, while bead mill homogenization was the superior technique for isolation of active enzymes. A new effective method is proposed for isolation of secondary metabolites with the aid of bead milling of fungal mycelia. © 2006 Verlag der Zeitschrift für Naturforschung.
License of this version: CC BY-NC-ND 3.0
Document Type: article
Publishing status: publishedVersion
Issue Date: 2006
Appears in Collections:Naturwissenschaftliche Fakultät

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1 image of flag of Germany Germany 26 66.67%
2 image of flag of China China 5 12.82%
3 image of flag of United States United States 1 2.56%
4 image of flag of Sri Lanka Sri Lanka 1 2.56%
5 image of flag of Korea, Republic of Korea, Republic of 1 2.56%
6 image of flag of India India 1 2.56%
7 image of flag of Croatia Croatia 1 2.56%
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9 image of flag of Czech Republic Czech Republic 1 2.56%
10 image of flag of Brazil Brazil 1 2.56%

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