dc.identifier.uri | http://dx.doi.org/10.15488/2370 | |
dc.identifier.uri | http://www.repo.uni-hannover.de/handle/123456789/2396 | |
dc.contributor.author | Latza, Stefan | |
dc.contributor.author | Berger, Ralf Günter | |
dc.date.accessioned | 2017-11-24T07:40:26Z | |
dc.date.available | 2017-11-24T07:40:26Z | |
dc.date.issued | 1997 | |
dc.identifier.citation | Latza, S.; Berger, R.G.: 1-O-trans-Cinnamoyl-β-ᴅ -glucopyranose: Alcohol Cinnamoyltransferase Activity in Fruits of Cape Gooseberry (Physalis peruviana L.). In: Zeitschrift für Naturforschung - Section C Journal of Biosciences 52 (1997), Nr. 11-12, S. 747-755. DOI: https://doi.org/10.1515/znc-1997-11-1205 | |
dc.description.abstract | Methyl and ethyl cinnamate are aroma volatiles frequently occurring in fruits. Evidence was obtained that the enzymatic transfer of cinnamic acid to endogenous alcohols present in fruits (methanol, ethanol. 1-propanol) depended on energy-rich 1-O-glycosyl esters of cinnamic acid which served as acyl donor molecules. A putative l-O-trans-cinnamoyl-β-D-gluco-pyranose: alcohol cinnamoyltransferase from cape gooseberry (Physalis peruviana L.) was active towards l-O-trans-cinnamoyl-β-D-glucopyranose and l-O-trans-cinnamoyl-β-D-gentio-biose. The enzyme was purified 290-fold by a protocol including ammonium sulphate precipitation, solubilization by Triton X-100, gel permeation and affinity chromatography on conca-navalin A. The acidic glycoprotein (pI = 4.8) most probably is membrane bound. The distribution of alcohol cinnamoyltransferase activity in gel chromatography fractions suggests a native Mrof 75,000. For l-O-trans-cinnamoyl-β-D-glucopyranose, an apparent Kmof 69 was determined. At pH > 6.0, non-enzymatic transesterification superposes the enzymatic transformation. | eng |
dc.language.iso | eng | |
dc.publisher | Berlin : De Gruyter | |
dc.relation.ispartofseries | Zeitschrift für Naturforschung - Section C Journal of Biosciences 52 (1997), Nr. 11-12 | |
dc.rights | CC BY-NC-ND 3.0 Unported | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ | |
dc.subject | Aroma Ester | eng |
dc.subject | Flavour Precursor | eng |
dc.subject | Glycoconjugate | eng |
dc.subject | Methyl Cinnamate | eng |
dc.subject | Phenylpropanoid Metabolism | eng |
dc.subject | Physalis peruviana | eng |
dc.subject.ddc | 570 | Biowissenschaften, Biologie | ger |
dc.title | 1-O-trans-Cinnamoyl-β-ᴅ -glucopyranose: Alcohol Cinnamoyltransferase Activity in Fruits of Cape Gooseberry (Physalis peruviana L.) | eng |
dc.type | Article | |
dc.type | Text | |
dc.relation.essn | 1865-7125 | |
dc.relation.issn | 0939-5075 | |
dc.relation.doi | https://doi.org/10.1515/znc-1997-11-1205 | |
dc.bibliographicCitation.issue | 11-12 | |
dc.bibliographicCitation.volume | 52 | |
dc.bibliographicCitation.firstPage | 747 | |
dc.bibliographicCitation.lastPage | 755 | |
dc.description.version | publishedVersion | |
tib.accessRights | frei zug�nglich |
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