1-O-trans-Cinnamoyl-β-ᴅ -glucopyranose: Alcohol Cinnamoyltransferase Activity in Fruits of Cape Gooseberry (Physalis peruviana L.)

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Latza, S.; Berger, R.G.: 1-O-trans-Cinnamoyl-β-ᴅ -glucopyranose: Alcohol Cinnamoyltransferase Activity in Fruits of Cape Gooseberry (Physalis peruviana L.). In: Zeitschrift für Naturforschung - Section C Journal of Biosciences 52 (1997), Nr. 11-12, S. 747-755. DOI: https://doi.org/10.1515/znc-1997-11-1205

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To cite the version in the repository, please use this identifier: https://doi.org/10.15488/2370

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Sum total of downloads: 239




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Abstract: 
Methyl and ethyl cinnamate are aroma volatiles frequently occurring in fruits. Evidence was obtained that the enzymatic transfer of cinnamic acid to endogenous alcohols present in fruits (methanol, ethanol. 1-propanol) depended on energy-rich 1-O-glycosyl esters of cinnamic acid which served as acyl donor molecules. A putative l-O-trans-cinnamoyl-β-D-gluco-pyranose: alcohol cinnamoyltransferase from cape gooseberry (Physalis peruviana L.) was active towards l-O-trans-cinnamoyl-β-D-glucopyranose and l-O-trans-cinnamoyl-β-D-gentio-biose. The enzyme was purified 290-fold by a protocol including ammonium sulphate precipitation, solubilization by Triton X-100, gel permeation and affinity chromatography on conca-navalin A. The acidic glycoprotein (pI = 4.8) most probably is membrane bound. The distribution of alcohol cinnamoyltransferase activity in gel chromatography fractions suggests a native Mrof 75,000. For l-O-trans-cinnamoyl-β-D-glucopyranose, an apparent Kmof 69 was determined. At pH > 6.0, non-enzymatic transesterification superposes the enzymatic transformation.
License of this version: CC BY-NC-ND 3.0 Unported
Document Type: article
Publishing status: publishedVersion
Issue Date: 1997
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
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1 image of flag of Germany Germany 175 73.22%
2 image of flag of United States United States 33 13.81%
3 image of flag of China China 11 4.60%
4 image of flag of India India 4 1.67%
5 image of flag of Russian Federation Russian Federation 3 1.26%
6 image of flag of Philippines Philippines 2 0.84%
7 image of flag of France France 2 0.84%
8 image of flag of Vietnam Vietnam 1 0.42%
9 image of flag of No geo information available No geo information available 1 0.42%
10 image of flag of Brazil Brazil 1 0.42%
    other countries 6 2.51%

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