Random amplified polymorphic DNA (RAPD) finger prints evidencing high genetic variability among marine angiosperms of India

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dc.identifier.uri http://dx.doi.org/10.15488/2345
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/2371
dc.contributor.author Dilipan, Elangovan
dc.contributor.author Papenbrock, Jutta
dc.contributor.author Thangaradjou, Thirunavakkasu
dc.date.accessioned 2017-11-17T10:07:38Z
dc.date.available 2018-05-20T22:05:14Z
dc.date.issued 2016
dc.identifier.citation Dilipan, E.; Papenbrock, J.; Thangaradjou, T.: Random amplified polymorphic DNA (RAPD) finger prints evidencing high genetic variability among marine angiosperms of India. In: Journal of the Marine Biological Association of the United Kingdom 97 (2016), Nr. 6, S. 1307-1315. DOI: https://doi.org/10.1017/S0025315416000631
dc.description.abstract In India 14 seagrass species can be found with monospecific genera (Enhalus, Thalassia and Syringodium), Cymodocea with two species and Halophila and Halodule represented by more than two taxonomically complex species. Considering this, the present study was made to understand the level and pattern of genetic variability among these species collected from Tamilnadu coast, India. Random amplified polymorphic DNA (RAPD) analysis was used to evaluate the level of polymorphism existing between the species. Out of the 12 primers tested, 10 primers amplified 415 DNA fragments with an average of 41.5 fragments per primer. Of the total 415 amplified fragments only 123 (29.7%) were monomorphic and the remaining 292 (70.3%) were polymorphic for Indian seagrass species. Among the 10 primers used four are identified as the key primers capable of distinguishing all the Indian seagrasses with a high degree of polymorphism and bringing representative polymorphic alleles in all the tested seagrasses. From the present investigation, this study shows that the RAPD marker technique can be used not only as a tool to analyse genetic diversity but also to resolve the taxonomic uncertainties existing in the Indian seagrasses. The efficiency of these primers in bringing out the genetic polymorphism or homogeneity among different populations of the Halophila and Halodule complex still has to be tested before recommending these primers as an identification tool for Indian seagrasses. Copyright © Marine Biological Association of the United Kingdom 2016 eng
dc.language.iso eng
dc.publisher Cambridge : Cambridge University Press
dc.relation.ispartofseries Journal of the Marine Biological Association of the United Kingdom 97 (2016), Nr. 6
dc.rights Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
dc.subject genetic variability eng
dc.subject India eng
dc.subject Marine angiosperm eng
dc.subject RAPD eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Random amplified polymorphic DNA (RAPD) finger prints evidencing high genetic variability among marine angiosperms of India
dc.type article
dc.type Text
dc.relation.issn 0025-3154
dc.relation.doi https://doi.org/10.1017/S0025315416000631
dc.bibliographicCitation.issue 6
dc.bibliographicCitation.volume 97
dc.bibliographicCitation.firstPage 1307
dc.bibliographicCitation.lastPage 1315
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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