Random amplified polymorphic DNA (RAPD) finger prints evidencing high genetic variability among marine angiosperms of India

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Dilipan, E.; Papenbrock, J.; Thangaradjou, T.: Random amplified polymorphic DNA (RAPD) finger prints evidencing high genetic variability among marine angiosperms of India. In: Journal of the Marine Biological Association of the United Kingdom 97 (2016), Nr. 6, S. 1307-1315. DOI: https://doi.org/10.1017/S0025315416000631

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Abstract: 
In India 14 seagrass species can be found with monospecific genera (Enhalus, Thalassia and Syringodium), Cymodocea with two species and Halophila and Halodule represented by more than two taxonomically complex species. Considering this, the present study was made to understand the level and pattern of genetic variability among these species collected from Tamilnadu coast, India. Random amplified polymorphic DNA (RAPD) analysis was used to evaluate the level of polymorphism existing between the species. Out of the 12 primers tested, 10 primers amplified 415 DNA fragments with an average of 41.5 fragments per primer. Of the total 415 amplified fragments only 123 (29.7%) were monomorphic and the remaining 292 (70.3%) were polymorphic for Indian seagrass species. Among the 10 primers used four are identified as the key primers capable of distinguishing all the Indian seagrasses with a high degree of polymorphism and bringing representative polymorphic alleles in all the tested seagrasses. From the present investigation, this study shows that the RAPD marker technique can be used not only as a tool to analyse genetic diversity but also to resolve the taxonomic uncertainties existing in the Indian seagrasses. The efficiency of these primers in bringing out the genetic polymorphism or homogeneity among different populations of the Halophila and Halodule complex still has to be tested before recommending these primers as an identification tool for Indian seagrasses. Copyright © Marine Biological Association of the United Kingdom 2016
License of this version: Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
Document Type: article
Publishing status: publishedVersion
Issue Date: 2016
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
total perc.
1 image of flag of Germany Germany 54 44.26%
2 image of flag of India India 16 13.11%
3 image of flag of United States United States 13 10.66%
4 image of flag of Vietnam Vietnam 3 2.46%
5 image of flag of Thailand Thailand 3 2.46%
6 image of flag of France France 3 2.46%
7 image of flag of Egypt Egypt 3 2.46%
8 image of flag of China China 3 2.46%
9 image of flag of Pakistan Pakistan 2 1.64%
10 image of flag of Indonesia Indonesia 2 1.64%
    other countries 20 16.39%

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