Action potentials in primary osteoblasts and in the MG-63 osteoblast-like cell line

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dc.identifier.uri http://dx.doi.org/10.15488/1678
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/1703
dc.contributor.author Pangalos, Maria
dc.contributor.author Bintig, Willem
dc.contributor.author Schlingmann, Barbara
dc.contributor.author Feyerabend, Frank
dc.contributor.author Witte, Frank
dc.contributor.author Begandt, Daniela
dc.contributor.author Heisterkamp, Alexander
dc.contributor.author Ngezahayo, Anaclet
dc.date.accessioned 2017-07-04T10:06:08Z
dc.date.available 2017-07-04T10:06:08Z
dc.date.issued 2011
dc.identifier.citation Pangalos, M.; Bintig, W.; Schlingmann, B.; Feyerabend, F.; Witte, F. et al.: Action potentials in primary osteoblasts and in the MG-63 osteoblast-like cell line. In: Journal of Bioenergetics and Biomembranes 43 (2011), Nr. 3, S. 311-322. DOI: https://doi.org/10.1007/s10863-011-9354-7
dc.description.abstract Whole-cell patch-clamp analysis revealed a resting membrane potential of −60 mV in primary osteoblasts and in the MG-63 osteoblast-like cells. Depolarization-induced action potentials were characterized by duration of 60 ms, a minimal peak-to-peak distance of 180 ms, a threshold value of −20 mV and a repolarization between the spikes to −45 mV. Expressed channels were characterized by application of voltage pulses between −150 mV and 90 mV in 10 mV steps, from a holding potential of −40 mV. Voltages below −60 mV induced an inward current. Depolarizing voltages above −30 mV evoked two currents: (a) a fast activated and inactivated inward current at voltages between −30 and 30 mV, and (b) a delayed-activated outward current that was induced by voltages above −30 mV. Electrophysiological and pharmacological parameters indicated that hyperpolarization activated strongly rectifying K+ (Kir) channels, whereas depolarization activated tetrodotoxin sensitive voltage gated Na+ (Nav) channels as well as delayed, slowly activated, non-inactivating, and tetraethylammonium sensitive voltage gated K+ (Kv) channels. In addition, RT-PCR showed expression of Nav1.3, Nav1.4, Nav1.5, Nav1.6, Nav1.7, and Kir2.1, Kir2.3, and Kir2.4 as well as Kv2.1. We conclude that osteoblasts express channels that allow firing of action potentials. The final publication is available at Springer via https://doi.org/10.1007/s10863-011-9354-7. eng
dc.description.sponsorship BMBF/NANOTOME
dc.description.sponsorship DFG/Transregio37
dc.description.sponsorship Boehringer Ingelheim International GmbH
dc.description.sponsorship BMBF/NANOTOME
dc.description.sponsorship DFG/Transregio/37
dc.description.sponsorship Boehringer Ingelheim International GmbH
dc.language.iso eng
dc.publisher Heidelberg : Springer Verlag
dc.relation.ispartofseries Journal of Bioenergetics and Biomembranes 43 (2011), Nr. 3
dc.rights Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden.
dc.subject Osteoblasts eng
dc.subject MG-63 cells eng
dc.subject Action potential eng
dc.subject Kir eng
dc.subject Kv eng
dc.subject Nav eng
dc.subject RT-PCR eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Action potentials in primary osteoblasts and in the MG-63 osteoblast-like cell line eng
dc.type article
dc.type Text
dc.relation.issn 0145-479X
dc.relation.doi https://doi.org/10.1007/s10863-011-9354-7
dc.bibliographicCitation.issue 3
dc.bibliographicCitation.volume 43
dc.bibliographicCitation.firstPage 311
dc.bibliographicCitation.lastPage 322
dc.description.version acceptedVersion
tib.accessRights frei zug�nglich


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