Action potentials in primary osteoblasts and in the MG-63 osteoblast-like cell line

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Pangalos, M.; Bintig, W.; Schlingmann, B.; Feyerabend, F.; Witte, F. et al.: Action potentials in primary osteoblasts and in the MG-63 osteoblast-like cell line. In: Journal of Bioenergetics and Biomembranes 43 (2011), Nr. 3, S. 311-322. DOI: https://doi.org/10.1007/s10863-011-9354-7

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Abstract: 
Whole-cell patch-clamp analysis revealed a resting membrane potential of −60 mV in primary osteoblasts and in the MG-63 osteoblast-like cells. Depolarization-induced action potentials were characterized by duration of 60 ms, a minimal peak-to-peak distance of 180 ms, a threshold value of −20 mV and a repolarization between the spikes to −45 mV. Expressed channels were characterized by application of voltage pulses between −150 mV and 90 mV in 10 mV steps, from a holding potential of −40 mV. Voltages below −60 mV induced an inward current. Depolarizing voltages above −30 mV evoked two currents: (a) a fast activated and inactivated inward current at voltages between −30 and 30 mV, and (b) a delayed-activated outward current that was induced by voltages above −30 mV. Electrophysiological and pharmacological parameters indicated that hyperpolarization activated strongly rectifying K+ (Kir) channels, whereas depolarization activated tetrodotoxin sensitive voltage gated Na+ (Nav) channels as well as delayed, slowly activated, non-inactivating, and tetraethylammonium sensitive voltage gated K+ (Kv) channels. In addition, RT-PCR showed expression of Nav1.3, Nav1.4, Nav1.5, Nav1.6, Nav1.7, and Kir2.1, Kir2.3, and Kir2.4 as well as Kv2.1. We conclude that osteoblasts express channels that allow firing of action potentials. The final publication is available at Springer via https://doi.org/10.1007/s10863-011-9354-7.
License of this version: Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden.
Document Type: article
Publishing status: acceptedVersion
Issue Date: 2011
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
total perc.
1 image of flag of Germany Germany 275 82.58%
2 image of flag of United States United States 31 9.31%
3 image of flag of United Kingdom United Kingdom 5 1.50%
4 image of flag of Ukraine Ukraine 3 0.90%
5 image of flag of China China 3 0.90%
6 image of flag of Russian Federation Russian Federation 2 0.60%
7 image of flag of Japan Japan 2 0.60%
8 image of flag of France France 2 0.60%
9 image of flag of Canada Canada 2 0.60%
10 image of flag of Austria Austria 2 0.60%
    other countries 6 1.80%

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