dc.identifier.uri |
http://dx.doi.org/10.15488/16055 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/16182 |
|
dc.contributor.author |
Heene, Sebastian
|
|
dc.contributor.author |
Renzelmann, Jannis
|
|
dc.contributor.author |
Müller, Caroline
|
|
dc.contributor.author |
Stanislawski, Nils
|
|
dc.contributor.author |
Cholewa, Fabian
|
|
dc.contributor.author |
Moosmann, Pia
|
|
dc.contributor.author |
Blume, Holger
|
|
dc.contributor.author |
Blume, Cornelia
|
|
dc.date.accessioned |
2024-01-25T11:11:31Z |
|
dc.date.available |
2024-01-25T11:11:31Z |
|
dc.date.issued |
2023 |
|
dc.identifier.citation |
Heene, S.; Renzelmann, J.; Müller, C.; Stanislawski, N.; Cholewa, F. et al.: A promising protocol for the endothelialization of vascular grafts in an instrumented rotating bioreactor towards clinical application. In: Biochemical Engineering Journal 200 (2023), 109095. DOI: https://doi.org/10.1016/j.bej.2023.109095 |
|
dc.description.abstract |
Pre-endothelialization of a tissue-engineered vascular graft before implantation aims to prevent thrombosis and immunoreactions. This work demonstrates a standardized cultivation process to build a confluent monolayer with human aortal endothelial cells on xenogenous scaffolds. Pre-tested dynamic cultivation conditions in flow slides with pulsatile flow (1 Hz) representing arterial wall conditions were transferred to a newly designed multi-featured rotational bioreactor system. The medium was thickened with 1% methyl cellulose simulating a non-Newtonian fluid comparable to blood. Computational fluid dynamics was used to estimate the optimal volume flow and medium distribution inside the bioreactor chamber for defined wall-near shear stress levels. Flow measurements were performed during cultivation for constant monitoring of the process. Three decellularized porcine arteries were seeded and cultivated in the bioreactor over six days. 1% MC turned out to be the optimal percentage to achieve shear stress values ranging up to 10 dyn/cm2. Vascular endothelial cells formed a continuous monolayer with significant cell alignment in the direction of flow. The presented cultivation protocol in the bioreactor system thus displays a promising template for graft endothelialization and cultivation. Therefore, establishing a key step for future tissue-engineered vascular graft development with a view towards clinical application. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Amsterdam [u.a.] : Elsevier |
|
dc.relation.ispartofseries |
Biochemical Engineering Journal 200 (2023) |
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dc.rights |
CC BY-NC-ND 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0 |
|
dc.subject |
Bioreactor system |
eng |
dc.subject |
Cell alignment |
eng |
dc.subject |
Dynamic cultivation |
eng |
dc.subject |
Endothelialization |
eng |
dc.subject |
Shear stress |
eng |
dc.subject |
Vascular graft |
eng |
dc.subject.ddc |
660 | Technische Chemie
|
|
dc.subject.ddc |
540 | Chemie
|
|
dc.title |
A promising protocol for the endothelialization of vascular grafts in an instrumented rotating bioreactor towards clinical application |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
1873-295X |
|
dc.relation.issn |
1369-703X |
|
dc.relation.doi |
https://doi.org/10.1016/j.bej.2023.109095 |
|
dc.bibliographicCitation.volume |
200 |
|
dc.bibliographicCitation.firstPage |
109095 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|