Poly-epsilon-caprolactone Coated and Functionalized Porous Titanium and Magnesium Implants for Enhancing Angiogenesis in Critically Sized Bone Defects

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dc.identifier.uri http://dx.doi.org/10.15488/1587
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/1612
dc.contributor.author Roland, Laura
dc.contributor.author Grau, Michael
dc.contributor.author Matena, Julia
dc.contributor.author Teske, Michael
dc.contributor.author Gieseke, Matthias
dc.contributor.author Kampmann, Andreas
dc.contributor.author Beyerbach, Martin
dc.contributor.author Murua Escobar, Hugo
dc.contributor.author Haferkamp, Heinz
dc.contributor.author Gellrich, Nils-Claudius
dc.contributor.author Nolte, Ingo
dc.date.accessioned 2017-05-31T11:16:23Z
dc.date.available 2017-05-31T11:16:23Z
dc.date.issued 2015
dc.identifier.citation Roland, Laura; Grau, Michael; Matena, Julia; Teske, Michael; Gieseke, Matthias et al.: Poly-epsilon-caprolactone Coated and Functionalized Porous Titanium and Magnesium Implants for Enhancing Angiogenesis in Critically Sized Bone Defects. In: International Journal of Molecular Sciences 17 (2015), Nr. 1, 1. DOI: https://doi.org/10.3390/ijms17010001
dc.description.abstract For healing of critically sized bone defects, biocompatible and angiogenesis supporting implants are favorable. Murine osteoblasts showed equal proliferation behavior on the polymers poly-epsilon-caprolactone (PCL) and poly-(3-hydroxybutyrate)/poly-(4-hydroxybutyrate) (P(3HB)/P(4HB)). As vitality was significantly better for PCL, it was chosen as a suitable coating material for further experiments. Titanium implants with 600 microm pore size were evaluated and found to be a good implant material for bone, as primary osteoblasts showed a vitality and proliferation onto the implants comparable to well bottom (WB). Pure porous titanium implants and PCL coated porous titanium implants were compared using Live Cell Imaging (LCI) with Green fluorescent protein (GFP)-osteoblasts. Cell count and cell covered area did not differ between the implants after seven days. To improve ingrowth of blood vessels into porous implants, proangiogenic factors like Vascular Endothelial Growth Factor (VEGF) and High Mobility Group Box 1 (HMGB1) were incorporated into PCL coated, porous titanium and magnesium implants. An angiogenesis assay was performed to establish an in vitro method for evaluating the impact of metallic implants on angiogenesis to reduce and refine animal experiments in future. Incorporated concentrations of proangiogenic factors were probably too low, as they did not lead to any effect. Magnesium implants did not yield evaluable results, as they led to pH increase and subsequent cell death. eng
dc.description.sponsorship DFG/299/11-1
dc.language.iso eng
dc.publisher Basel : MDPI AG
dc.relation.ispartofseries International Journal of Molecular Sciences 17 (2015), Nr. 1
dc.rights CC BY 4.0
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject 0 (HMGB1 Protein) eng
dc.subject 0 (Hydroxybutyrates) eng
dc.subject 0 (Polyesters) eng
dc.subject 0 (Vascular Endothelial Growth Factor A) eng
dc.subject 117068-64-1 (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)) eng
dc.subject 24980-41-4 (polycaprolactone) eng
dc.subject Animals eng
dc.subject Bone-Implant Interface/blood supply eng
dc.subject Cell Line eng
dc.subject Cells, Cultured eng
dc.subject D1JT611TNE (Titanium) eng
dc.subject HMGB1 Protein/pharmacology eng
dc.subject Hydroxybutyrates/adverse effects/pharmacology eng
dc.subject I38ZP9992A (Magnesium) eng
dc.subject Magnesium/adverse effects/pharmacology eng
dc.subject Mice eng
dc.subject Mice, Inbred C57BL eng
dc.subject Neovascularization, Physiologic eng
dc.subject Osseointegration eng
dc.subject Osteoblasts/drug effects/physiology eng
dc.subject Polyesters/adverse effects/pharmacology eng
dc.subject Porosity eng
dc.subject Titanium/adverse effects/pharmacology eng
dc.subject Vascular Endothelial Growth Factor A/pharmacology eng
dc.subject.ddc 540 | Chemie ger
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Poly-epsilon-caprolactone Coated and Functionalized Porous Titanium and Magnesium Implants for Enhancing Angiogenesis in Critically Sized Bone Defects
dc.type article
dc.type Text
dc.relation.issn 1422-0067
dc.relation.doi https://doi.org/10.3390/ijms17010001
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 17
dc.bibliographicCitation.firstPage 1
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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