Determination of aqueous two-phase system phase-forming components in the presence of bovine serum albumin

Originalpublikation

Glyk, A.; Scheper, T.; Beutel, S.: Determination of aqueous two-phase system phase-forming components in the presence of bovine serum albumin. In: Analytical Biochemistry 455 (2014), Nr. 1, S. 10-12. DOI: https://doi.org/10.1016/j.ab.2014.03.009

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Zusammenfassung:
In the current work, the quantification of different poly(ethylene glycol) (PEG)-potassium phosphate/sodium citrate aqueous two-phase system (ATPS) phase-forming components was investigated by using conductivity and refractive index measurements. For this purpose, refractive index and conductivity calibration curves were obtained for ATPS at different pH values in the presence of different bovine serum albumin (BSA) concentrations. Whereas BSA had no effect on the conductivity, it had a considerable effect on the refractive index. Finally, a convenient dilution of the samples prior to the ATPS constituent determination is needed to ensure no significant influence from BSA.
Lizenzbestimmungen: CC BY-NC-ND 4.0 Unported - https://creativecommons.org/licenses/by-nc-nd/4.0/
Publikationstyp: Article
Publikationsstatus: acceptedVersion
Erstveröffentlichung: 2014
Schlagwörter (englisch): Aqueous two-phase system, Conductivity measurement, Refractive index measurement
Fachliche Zuordnung (DDC): 500 | Naturwissenschaften, 570 | Biowissenschaften, Biologie, 540 | Chemie

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