Determination of aqueous two-phase system phase-forming components in the presence of bovine serum albumin

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Glyk, A.; Scheper, T.; Beutel, S.: Determination of aqueous two-phase system phase-forming components in the presence of bovine serum albumin. In: Analytical Biochemistry 455 (2014), Nr. 1, S. 10-12. DOI: https://doi.org/10.1016/j.ab.2014.03.009

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Abstract: 
In the current work, the quantification of different poly(ethylene glycol) (PEG)-potassium phosphate/sodium citrate aqueous two-phase system (ATPS) phase-forming components was investigated by using conductivity and refractive index measurements. For this purpose, refractive index and conductivity calibration curves were obtained for ATPS at different pH values in the presence of different bovine serum albumin (BSA) concentrations. Whereas BSA had no effect on the conductivity, it had a considerable effect on the refractive index. Finally, a convenient dilution of the samples prior to the ATPS constituent determination is needed to ensure no significant influence from BSA.
License of this version: CC BY-NC-ND 4.0 Unported
Document Type: article
Publishing status: acceptedVersion
Issue Date: 2014
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
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1 image of flag of Germany Germany 79 66.95%
2 image of flag of United States United States 7 5.93%
3 image of flag of Iran, Islamic Republic of Iran, Islamic Republic of 7 5.93%
4 image of flag of India India 4 3.39%
5 image of flag of Europe Europe 4 3.39%
6 image of flag of China China 3 2.54%
7 image of flag of Russian Federation Russian Federation 2 1.69%
8 image of flag of Bulgaria Bulgaria 2 1.69%
9 image of flag of Argentina Argentina 2 1.69%
10 image of flag of Vietnam Vietnam 1 0.85%
    other countries 7 5.93%

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