dc.identifier.uri |
http://dx.doi.org/10.15488/11215 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/11301 |
|
dc.contributor.author |
Günther, Thorben
|
|
dc.contributor.author |
Schoppe, Lasse
|
|
dc.contributor.author |
Ersoy, Franziska
|
|
dc.contributor.author |
Berger, Ralf G.
|
|
dc.date.accessioned |
2021-08-13T07:43:53Z |
|
dc.date.available |
2021-08-13T07:43:53Z |
|
dc.date.issued |
2021 |
|
dc.identifier.citation |
Günther, T.; Schoppe, L.; Ersoy, F.; Berger, R.G.: Alternative Routes for the Production of Natural 4-Vinylguaiacol from Sugar Beet Fiber Using Basidiomycetous Enzymes. In: Catalysts : open access journal 11 (2021), Nr. 5, 631. DOI: https://doi.org/10.3390/catal11050631 |
|
dc.description.abstract |
Traditional smoking generates not only the impact flavor compound 4-vinylguaiacol, but concurrently many unwanted and potent toxic compounds such as polycyclic aromatic hydrocarbons. Enzyme technology provides a solution without any side-product formation. A feruloyl esterase from Rhizoctonia solani (RspCAE) liberated ferulic acid from low-priced sugar beet fiber. Decarboxylation of ferulic acid to 4-vinylguaiacol was achieved by a second enzyme from Schizophyllum commune (ScoFAD). Both enzymes were covalently immobilized on agarose to enable reusability in a fixed-bed approach. The two enzyme cascades showed high conversion rates with yields of 0.8 and 0.95, respectively, and retained activity for nearly 80 h of continuous operation. The overall productivity of the model process with bed volumes of 300 µL and a substrate flow rate of 0.25 mL min−1 was 3.98 mg 4-vinylguaiacol per hour. A cold online solid phase extraction using XAD4 was integrated into the bioprocess and provided high recovery rates during multiple elution steps. Attempting to facilitate the bioprocess, a fused gene coding for the two enzymes and a set of different linker lengths and properties was constructed and introduced into Komagataella phaffii. Longer and rigid linkers resulted in higher activity of the fusion protein with a maximum of 67 U L−1 |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Basel : MDPI |
|
dc.relation.ispartofseries |
Catalysts : open access journal 11 (2021), Nr. 5 |
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dc.rights |
CC BY 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
smoke flavor |
eng |
dc.subject |
4-vinylguaiacol |
eng |
dc.subject |
bioprocess |
eng |
dc.subject |
immobilization |
eng |
dc.subject |
basidiomycetes |
eng |
dc.subject |
fusion protein |
eng |
dc.subject.ddc |
540 | Chemie
|
ger |
dc.title |
Alternative Routes for the Production of Natural 4-Vinylguaiacol from Sugar Beet Fiber Using Basidiomycetous Enzymes |
|
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
2073-4344 |
|
dc.relation.doi |
https://doi.org/10.3390/catal11050631 |
|
dc.bibliographicCitation.issue |
5 |
|
dc.bibliographicCitation.volume |
11 |
|
dc.bibliographicCitation.firstPage |
631 |
|
dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
|