dc.identifier.uri |
http://dx.doi.org/10.15488/10337 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/10410 |
|
dc.contributor.author |
Detering, Thorben
|
|
dc.contributor.author |
Mundry, Katharina
|
|
dc.contributor.author |
Berger, Ralf G.
|
|
dc.date.accessioned |
2021-01-20T13:15:03Z |
|
dc.date.available |
2021-01-20T13:15:03Z |
|
dc.date.issued |
2020 |
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dc.identifier.citation |
Detering, T.; Mundry, K.; Berger, R.G.: Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants. In: PLoS ONE 15 (2020), Nr. 12, e0244290. DOI: https://doi.org/10.1371/journal.pone.0244290 |
|
dc.description.abstract |
Traditional smoke flavours bear the risk of containing a multitude of contaminating carcinogenic side-products. Enzymatic decarboxylation of ferulic acid released from agro-industrial side-streams by ferulic acid esterases (FAE) enables the sustainable generation of pure, food grade 4-vinylguaiacol (4-VG), the impact compound of smoke flavour. The first basidiomycetous ferulic acid decarboxylase (FAD) was isolated from Schizophyllum commune (ScoFAD) and heterologously produced by Komagataella phaffii. It showed a molecular mass of 21 kDa, catalytic optima at pH 5.5 and 35 C, and a sequence identity of 63.6% to its next relative, a FAD from the ascomycete Cordyceps farinosa. The ScoFAD exhibited a high affinity to its only known substrate ferulic acid (FA) of 0.16 mmol L-1 and a turnover number of 750 s-1. The resulting catalytic efficiency kcat KM-1 of 4,779 L s-1 mmol-1 exceeded the next best known enzyme by more than a factor of 50. Immobilised on AminoLink Plus Agarose, ScoFAD maintained its activity for several days. The combination with FAEs and agro-industrial side-streams paves the way for a new generation of sustainable, clean, and safe smoke flavours. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
San Francisco, CA : Public Library of Science |
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dc.relation.ispartofseries |
PLoS ONE 15 (2020), Nr. 12 |
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dc.rights |
CC BY 4.0 Unported |
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dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
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dc.subject |
smoke flavours |
eng |
dc.subject |
cancer |
eng |
dc.subject |
ferulic acid decarboxylase |
eng |
dc.subject.ddc |
540 | Chemie
|
ger |
dc.title |
Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.doi |
10.1371/journal.pone.0244290 |
|
dc.bibliographicCitation.issue |
12 |
|
dc.bibliographicCitation.volume |
15 |
|
dc.bibliographicCitation.firstPage |
e0244290 |
|
dc.description.version |
publishedVersion |
eng |
tib.accessRights |
frei zug�nglich |
|