Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants

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Detering, T.; Mundry, K.; Berger, R.G.: Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants. In: PLoS ONE 15 (2020), Nr. 12, e0244290. DOI:

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Sum total of downloads: 79

Traditional smoke flavours bear the risk of containing a multitude of contaminating carcinogenic side-products. Enzymatic decarboxylation of ferulic acid released from agro-industrial side-streams by ferulic acid esterases (FAE) enables the sustainable generation of pure, food grade 4-vinylguaiacol (4-VG), the impact compound of smoke flavour. The first basidiomycetous ferulic acid decarboxylase (FAD) was isolated from Schizophyllum commune (ScoFAD) and heterologously produced by Komagataella phaffii. It showed a molecular mass of 21 kDa, catalytic optima at pH 5.5 and 35 C, and a sequence identity of 63.6% to its next relative, a FAD from the ascomycete Cordyceps farinosa. The ScoFAD exhibited a high affinity to its only known substrate ferulic acid (FA) of 0.16 mmol L-1 and a turnover number of 750 s-1. The resulting catalytic efficiency kcat KM-1 of 4,779 L s-1 mmol-1 exceeded the next best known enzyme by more than a factor of 50. Immobilised on AminoLink Plus Agarose, ScoFAD maintained its activity for several days. The combination with FAEs and agro-industrial side-streams paves the way for a new generation of sustainable, clean, and safe smoke flavours.
License of this version: CC BY 4.0 Unported
Document Type: Article
Publishing status: publishedVersion
Issue Date: 2020
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
total perc.
1 image of flag of Germany Germany 51 64.56%
2 image of flag of United States United States 13 16.46%
3 image of flag of China China 3 3.80%
4 image of flag of Tanzania, United Republic of Tanzania, United Republic of 2 2.53%
5 image of flag of Brazil Brazil 2 2.53%
6 image of flag of Vietnam Vietnam 1 1.27%
7 image of flag of Taiwan Taiwan 1 1.27%
8 image of flag of Russian Federation Russian Federation 1 1.27%
9 image of flag of Canada Canada 1 1.27%
10 image of flag of Australia Australia 1 1.27%
    other countries 3 3.80%

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