Brämers, C.; Lammers, F.; Scheper, T.; Beutel, S.: Development and Testing of a 4-Columns Periodic Counter-Current Chromatography System Based on Membrane Adsorbers. In: Separations 6 (2019), Nr. 4, 55. DOI: https://doi.org/10.3390/separations6040055
Abstract: | |
Continuous chromatography can surmount the disadvantages of batch chromatography like low productivities and extensive usage of consumables. In this work, a 4-column continuous chromatographic system based on the principle of periodic counter-current chromatography (PCCC) was developed and tested with a model protein mixture of BSA and lysozyme. The PCCC system was specially designed for membrane adsorbers as an alternative to conventional columns to facilitate the use of disposable process units and to further increase the productivity due to higher convective mass transport in the membrane adsorber. Membrane adsorber Sartobind® Q was used to continuously purify BSA from the protein mixture. The usage of PCCC led to an increased capacity utilization (here 20%) and higher space–time-yields, and thus to a remarkable productivity increase and cost savings. | |
License of this version: | CC BY 4.0 Unported |
Document Type: | Article |
Publishing status: | publishedVersion |
Issue Date: | 2019 |
Appears in Collections: | Naturwissenschaftliche Fakultät |
pos. | country | downloads | ||
---|---|---|---|---|
total | perc. | |||
1 | Germany | 104 | 68.87% | |
2 | United States | 28 | 18.54% | |
3 | China | 5 | 3.31% | |
4 | No geo information available | 3 | 1.99% | |
5 | Russian Federation | 3 | 1.99% | |
6 | Taiwan | 1 | 0.66% | |
7 | Netherlands | 1 | 0.66% | |
8 | India | 1 | 0.66% | |
9 | Indonesia | 1 | 0.66% | |
10 | Switzerland | 1 | 0.66% | |
other countries | 3 | 1.99% |
Hinweis
Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.