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Characterization of the bifunctional cytochrome c reductase-processing peptidase complex from potato mitochondria

Emmermann, M.; Braun, Hans-Peter; Arretz, M.; Schmitz, U.K. (Bethesda, Md. : ASBMB Publications, 1993)

In potato, cytochrome c reductase, a protein complex of the respiratory chain, exhibits processing activity toward mitochondrial precursor proteins. One of the two cooperating components of the processing peptidase was ...
Comparison of colorimetric methods for the quantification of model proteins in aqueous two-phase systems

Glyk, Anna; Heinisch, Sandra L.; Scheper, Thomas; Beutel, Sascha (Cambridge, MA : Elsevier Academic Press, 2015)

In the current study, the quantification of different model proteins in the presence of typical aqueous two-phase system components was investigated by using the Bradford and bicinchoninic acid (BCA) assays. Each phase-forming ...
Complexome profiling reveals association of PPR proteins with ribosomes in the mitochondria of plants

Rugen, Nils; Straube, Henryk; Franken, Linda E.; Braun, Hans-Peter; Eubel, Holger (Bethesda, Md. : The American Society for Biochemistry and Molecular Biology, 2019)

Mitochondrial transcripts are subject to a wealth of processing mechanisms including cis- and trans-splicing events, as well as base modifications (RNA editing). HUndreds of proteins are required for these processes in ...
MXene molecular sieving membranes for highly efficient gas separation

Ding, Li; Wei, Yanying; Li, Libo; Zhang, Tao; Wang, Haihui; Xue, Jian; Ding, Liang-Xin; Wang, Suqing; Caro, Jürgen; Gogotsi, Yury (London : Nature Publishing Group, 2018)

Molecular sieving membranes with sufficient and uniform nanochannels that break the permeability-selectivity trade-off are desirable for energy-efficient gas separation, and the arising two-dimensional (2D) materials provide ...
The native structure and composition of the cruciferin complex in brassica napus

Nietzel, Thomas; Dudkina, Natalya V.; Haase, Christin; Denolf, Peter; Semchonok, Dmitry A.; Boekema, Egbert J.; Braun, Hans-Peter; Sunderhaus, Stephanie (Bethesda, Md. : ASBMB Publications, 2013)

Globulins are an important group of seed storage proteins in dicotyledonous plants. They are synthesized during seed development, assembled into very compact protein complexes, and finally stored in protein storage vacuoles ...
Purification and partial characterization of an extracellular melanoprotein from the fungus Venturia inaequalis

Singh, P.; Piotrowski, M.; Gau, A.E. (Berlin : De Gruyter, 2005)

The fungus Venturia inaequalis clone No. 36 isolated from Malus domestica cv. Gloster excretes a melanoprotein of 36 kDa in relatively high amounts during growth in liquid culture. The protein was isolated from the culture ...
Single-use membrane adsorbers for endotoxin removal and purification of endogenous polysialic acid from Escherichia coli K1

de Vries, Ingo; Schreiber, Sarah; Boßmann, Daniel; Hellmann, Zawadi; Kopatz, Jens; Neumann, Harald; Beutel, Sascha (Amsterdam : Elsevier B.V., 2018)

Polysialic acid (polySia) is a promising molecule for various medical applications (e.g., treatment of inflammatory neurodegenerative diseases). In this study a complete production process for human-identical α-(2,8)-linked ...
Structural Basis for Regulation of the Opposing (p)ppGpp Synthetase and Hydrolase within the Stringent Response Orchestrator Rel

Pausch, Patrick; Abdelshahid, Maha; Steinchen, Wieland; Schäfer, Heinrich; Gratani, Fabio Lino; Freibert, Sven-Andreas.; Wolz, Christiane; Turgay, Kürşad; Wilson, Daniel N.; Bange, Gert (New York, NY : Elsevier, 2020)

The stringent response enables metabolic adaptation of bacteria under stress conditions and is governed by RelA/SpoT Homolog (RSH)-type enzymes. Long RSH-type enzymes encompass an N-terminal domain (NTD) harboring the ...
Sulfurtransferase and thioredoxin specifically interact as demonstrated by bimolecular fluorescence complementation analysis and biochemical tests

Henne, Melina; König, Nicolas; Triulzi, Tiziana; Baroni, Sara; Forlani, Fabio; Scheibe, Renate; Papenbrock, Jutta (Amsterdam : Elsevier, 2015)

Sulfurtransferases (Strs) and thioredoxins (Trxs) are members of large protein families. Trxs are disulfide reductases and play an important role in redox-related cellular processes. They interact with a broad range of ...
SUMO-fusion, purification, and characterization of a (+)-zizaene synthase from Chrysopogon zizanioides

Hartwig, Steffen; Frister, Thore; Alemdar, Semra; Li, Zhaopeng; Scheper, Thomas; Beutel, Sascha (Cambridge, MA : Elsevier Academic Press, 2015)

An uncharacterized plant cDNA coding for a polypeptide presumably having sesquiterpene synthase activity, was expressed in soluble and active form. Two expression strategies were evaluated in Escherichia coli. The enzyme ...

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