Zusammenfassung: | |
Mitochondrial transcripts are subject to a wealth of processing mechanisms including cis- and trans-splicing events, as well as base modifications (RNA editing). HUndreds of proteins are required for these processes in plant mitochondria, many of which belong to the pentatricopeptide repeat (PPR) protein superfamily. The structure, localization, and function of these proteins is only poorly Understood. Here we present evidence that several PPR proteins are boUnd to mitoribosomes in plants. A novel complexome profiling strategy in combination with chemical crosslinking has been employed to systematically define the protein constituents of the large and the small ribosomal subunits in the mitochondria of plants. We identified more than 80 ribosomal proteins, which include several PPR proteins and other non-conventional ribosomal proteins. These findings reveal a potential coupling of transcriptional and translational events in the mitochondria of plants. Furthermore, the data indicate an extremely high molecular mass of the “small” subunit, even exceeding that of the “large” subunit.
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Lizenzbestimmungen: | CC BY 4.0 Unported - https://creativecommons.org/licenses/by/4.0/ |
Publikationstyp: | Article |
Publikationsstatus: | publishedVersion |
Erstveröffentlichung: | 2019 |
Schlagwörter (englisch): | plant protein, ribosome protein, Arabidopsis protein, mitochondrial protein, multiprotein complex, ribosome protein, Arabidopsis, Article, cross linking, density gradient centrifugation, gene expression, intracellular space, large ribosomal subunit, mitochondrial gene, mitochondrial ribosome, mitochondrion, nonhuman, priority journal, protein analysis, protein expression, ribosome, RNA editing, small ribosomal subunit, bacterium, metabolism, mitochondrion, molecular weight, plant leaf, protein subunit, proteomics, ribosome, Arabidopsis, Arabidopsis Proteins, Bacteria, Mitochondria, Mitochondrial Proteins, Molecular Weight, Multiprotein Complexes, Plant Leaves, Protein Subunits, Proteomics, Ribosomal Proteins, Ribosome Subunits, Large, Ribosome Subunits, Small, Ribosomes |
Fachliche Zuordnung (DDC): | 540 | Chemie, 610 | Medizin, Gesundheit |
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