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3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function

Timashev, P.S.; Vedunova, M.V.; Guseva, D.; Ponimaskin, E.; Deiwick, A.; Mishchenko, T.A.; Mitroshina, E.V.; Koroleva, A.V.; Pimashkin, A.S.; Mukhina, I.V.; Panchenko, V.Y.; Chichkov, Boris; Bagratashvili, Victor N. (Bristol : Institute of Physics Publishing, 2016)

Zr-Si organic-inorganic scaffolds fabricated by a two-photon polymerization technique were used for the primary culture of mouse embryonic neural cells. We observed that dissociated hippocampal cells adhere to the scaffolds, ...
Adhesion, Vitality and Osteogenic Differentiation Capacity of Adipose Derived Stem Cells Seeded on Nitinol Nanoparticle Coatings

Strauß, Sarah; Neumeister, Anne; Barcikowski, Stephan; Kracht, Dietmar; Kuhbier, Jörn W.; Radtke, Christine; Reimers, Kerstin; Vogt, Peter M. (San Francisco, California, US : PLOS, 2013)

Autologous cells can be used for a bioactivation of osteoimplants to enhance osseointegration. In this regard, adipose derived stem cells (ASCs) offer interesting perspectives in implantology because they are fast and easy ...
Die Bedeutung der Anaphylatoxine C3a und C5a im Mausmodell der allergischen Atemwegsentzündung

Bälder, Ralf (Hannover : Universität, 2004)

[no abstract]
Biodistribution, biocompatibility and targeted accumulation of magnetic nanoporous silica nanoparticles as drug carrier in orthopedics

Janßen, Hilke Catherina; Angrisani, Nina; Kalies, Stefan; Hansmann, Florian; Kietzmann, Manfred; Warwas, Dawid Peter; Behrens, Peter; Reifenrath, Janin (London : BioMed Central Ltd., 2020)

Background: In orthopedics, the treatment of implant-associated infections represents a high challenge. Especially, potent antibacterial effects at implant surfaces can only be achieved by the use of high doses of antibiotics, ...
Biofunctional quantum dots as fluorescence probe for cell-specific targeting

Ag, Didem; Bongartz, Rebecca; Dogan, Leyla Eral; Seleci, Muharrem; Walter, Johanna-Gabriela; Demirkol, Dilek Odaci; Stahl, Frank; Ozcelik, Serdar; Timur, Suna; Scheper, Thomas (Amsterdam : Elsevier, 2014)

We describe here the synthesis, characterization, bioconjugation, and application of water-soluble thioglycolic acid TGA-capped CdTe/CdS quantum dots (TGA-QDs) for targeted cellular imaging. Anti-human epidermal growth ...
CD14 and ALPK1 Affect Expression of Tight Junction Components and Proinflammatory Mediators upon Bacterial Stimulation in a Colonic 3D Organoid Model

Brooks, Pascal; Zur Bruegge, Talke; Boyle, Erin C.; Kalies, Stefan; Villarreal, Santiago Nahuel; Liese, Andrea; Bleich, André; Buettner, Manuela (London : Hindawi Limited, 2020)

Cd14 and Alpk1 both encode pathogen recognition receptors and are known candidate genes for affecting severity in inflammatory bowel diseases. CD14 acts as a coreceptor for bacterial lipopolysaccharide (LPS), while ALPK1 ...
Clastogenic Effect of Carthamus lanatus L. (Asteraceae)

Topashka-Ancheva, Margarita; Taskova, Rilka M.; Handjieva, Nedjalka; Mikhova, Bozhanka; Duddeck, Helmut (Berlin : De Gruyter, 2003)

The clastogenic effect of total dichloromethane, methanol and water extracts, four bioactive fractions and three individual constituents from Carthamus lanatus aerial parts were evaluated in mice by bone marrow chromosome ...
Combination therapies induce cancer cell death through the integrated stress response and disturbed pyrimidine metabolism

Hartleben, Goetz; Schorpp, Kenji; Kwon, Yun; Betz, Barbara; Tsokanos, Foivos-Filippos; Dantes, Zahra; Schäfer, Arlett; Rothenaigner, Ina; Monroy Kuhn, José Manuel; Morigny, Pauline; Mehr, Lisa; Lin, Sean; Seitz, Susanne; Tokarz, Janina; Artati, Anna; Adamsky, Jerzy; Plettenburg, Oliver; Lutter, Dominik; Irmler, Martin; Beckers, Johannes; Reichert, Maximilian; Hadian, Kamyar; Zeigerer, Anja; Herzig, Stephan; Berriel Diaz, Mauricio (Weinheim : Wiley-VCH, 2021)

By accentuating drug efficacy and impeding resistance mechanisms, combinatorial, multi-agent therapies have emerged as key approaches in the treatment of complex diseases, most notably cancer. Using high-throughput drug ...
EBIO Does Not Induce Cardiomyogenesis in Human Pluripotent Stem Cells but Modulates Cardiac Subtype Enrichment by Lineage-Selective Survival

Jara-Avaca, Monica; Kempf, Henning; Rückert, Michael; Robles-Diaz, Diana; Franke, Annika; de la Roche, Jeanne; Fischer, Martin; Malan, Daniela; Sasse, Philipp; Solodenko, Wladimir; Dräger, Gerald; Kirschning, Andreas; Martin, Ulrich; Zweigerdt, Robert (New York, NY : Springer, 2017)

Subtype-specific human cardiomyocytes (CMs) are valuable for basic and applied research. Induction of cardiomyogenesis and enrichment of nodal-like CMs was described for mouse pluripotent stem cells (mPSCs) in response to ...
Effects of degradable Mg-Ca alloys on dendritic cell function

Feser, K.; Kietzmann, M.; Bäumer, W.; Krause, C.; Bach, F.W. (London : SAGE Publications Ltd., 2011)

Degradable magnesium alloys are new materials for implants used in orthopedic and trauma surgery. The aim of this study was to investigate the influence of degradable magnesium alloys on the function of dendritic cells ...
Evaluation of incidence rates in pre-clinical studies using a williams-type procedure

Hothorn, Ludwig A.; Sill, Martin; Schaarschmidt, Frank (Berlin : De Gruyter, 2010)

The analysis of dose-response relationships is a common problem in pre-clinical studies. For example, proportions such as mortality rates and histopathological findings are of particular interest in repeated toxicity ...
Evaluation of the biological tolerability of the starch-based medical device 4DryField® PH in vitro and in vivo a rat model

Poehnert, Daniel; Abbas, Mahmoud; Maegel, Lavinia; Sambale, Franziska; Lavrentieva, Antonina; Kreipe, Hans-Heinrich; Klempnauer, Jürgen; Winny, Markus (London : SAGE Publications Ltd., 2015)

Purpose To evaluate in vitro cytotoxicity/biocompatibility as well as in vivo tolerability of the novel polysaccharide 4DryField® PH, certified for haemostasis and adhesion prevention. Methods In vitro cytotoxicity/viability ...
Free Bioverit® II implants coated with a nanoporous silica layer in a mouse ear model - A histological study

Vogt, Julia C.; Brandes, Gudrun; Ehlert, Nina; Behrens, Peter; Nolte, Ingo; Mueller, Peter P.; Lenarz, Thomas; Stieve, Martin (London : SAGE Publications Ltd., 2009)

The objective of this study is to evaluate the suitability of a mouse middle ear model for testing ossicular replacement materials. Twenty-four BALB/c mice are implanted with the bioglass-ceramic Bioverit® II which is ...
Immunogenicity of intensively decellularized equine carotid arteries is conferred by the extracellular matrix protein collagen type VI

Boeer, Ulrike; Buettner, Falk F.R.; Klingenberg, Melanie; Antonopoulos, Georgios C.; Meyer, Heiko; Haverich, Axel; Wilhelmi, Mathias (San Francisco, CA : Public Library of Science (PLoS), 2014)

The limited biocompatibility of decellularized scaffolds is an ongoing challenge in tissue engineering. Here, we demonstrate the residual immunogenicity of an extensively decellularized equine carotid artery (dEAC ...
Inner tegument proteins of Herpes Simplex Virus are sufficient for intracellular capsid motility in neurons but not for axonal targeting

Buch, Anna; Müller, Oliver; Ivanova, Lyudmila; Döhner, Katinka; Bialy, Dagmara; Bosse, Jens B.; Pohlmann, Anja; Binz, Anne; Hegemann, Maike; Nagel, Claus-Henning; Koltzenburg, Martin; Viejo-Borbolla, Abel; Rosenhahn, Bodo; Bauerfeind, Rudolf; Sodeik, Beate (San Francisco, CA : Public Library of Science (PLoS), 2017)

Upon reactivation from latency and during lytic infections in neurons, alphaherpesviruses assemble cytosolic capsids, capsids associated with enveloping membranes, and transport vesicles harboring fully enveloped capsids. ...
Magnetic beads enhance adhesion of NIH 3T3 fibroblasts: A proof-of-principle in vitro study for implant-mediated long-term drug delivery to the inner ear

Aliuos, Pooyan; Schulze, Jennifer; Schomaker, Markus; Reuter, Günter; Stolle, Stefan R.O.; Werner, Darja; Ripken, Tammo; Lenarz, Thomas; Warnecke, Athanasia (San Francisco, CA : Public Library of Science (PLoS), 2016)

Introduction Long-term drug delivery to the inner ear may be achieved by functionalizing cochlear implant (CI) electrodes with cells providing neuroprotective factors. However, effective strategies in order to coat implant ...
Method for 3D airway topology extraction

Grothausmann, Roman; Kellner, Manuela; Heidrich, Marko; Lorbeer, Raoul-Amadeus; Ripken, Tammo; Meyer, Heiko; Kuehnel, Mark P.; Ochs, Matthias; Rosenhahn, Bodo (New York, NY : Hindawi Publishing Corporation, 2015)

In lungs the number of conducting airway generations as well as bifurcation patterns varies across species and shows specific characteristics relating to illnesses or gene variations. A method to characterize the topology ...
Murine and human pluripotent stem cell-derived cardiac bodies form contractile myocardial tissue in vitro

Kensah, George; Lara, Angelica Roa; Dahlmann, Julia; Zweigerdt, Robert; Schwanke, Kristin; Hegermann, Jan; Skvorc, David; Gawol, Anke; Azizian, Azadeh; Wagner, Stefan; Maier, Lars S.; Krause, Andreas; Dräger, Gerald; Ochs, Matthias; Haverich, Axel; Gruh, Ina; Martin, Ulrich (Oxford : Oxford University Press, 2013)

AimsWe explored the use of highly purified murine and human pluripotent stem cell (PSC)-derived cardiomyocytes (CMs) to generate functional bioartificial cardiac tissue (BCT) and investigated the role of fibroblasts, ...
Platinum corrosion products from electrode contacts of human cochlear implants induce cell death in cell culture models

Wissel, Kirsten; Brandes, Gudrun; Pütz, Nils; Angrisani, Gian Luigi; Thieleke, Jan; Lenarz, Thomas; Durisin, Martin (San Francisco, CA : Public Library of Science, 2018)

Despite the technological progress made with cochlear implants (CI), impedances and their diagnosis remain a focus of interest. Increases in impedance have been related to technical defects of the electrode as well as ...
Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli

Rueda, Fabian; Céspedes, Maria Virtudes; Sánchez-Chardi, Alejandro; Seras-Franzoso, Joaquin; Pesarrodona, Mireia; Ferrer-Miralles, Neus; Vázquez, Esther; Rinas, Ursula; Unzueta, Ugutz; Mamat, Uwe; Mangues, Ramon; García-Fruitós, Elena; Villaverde, Antonio (London : BioMed Central Ltd., 2016)

Background: Production of recombinant drugs in process-friendly endotoxin-free bacterial factories targets to a lessened complexity of the purification process combined with minimized biological hazards during product ...

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