In this study, the nutrient regulation ecosystem service (ES) demand was quantified and mapped in an agriculturally-dominated landscape in the federal German state of North Rhine-Westphalia. The demand was assessed in a case study area on an individual field scale. As an indicator for the nutrient regulation ecosystem service demand, nitrogen budgets were calculated. The assessment includes a comparison of an agriculturally calculated nitrogen budget to an ecologically calculated nitrogen budget. The agricultural calculation is based on legal regulations and considers volatile nitrogen losses from fertilizers, whereas the ecological calculation incorporates the total amount of nitrogen and includes also the atmospheric nitrogen deposition. Furthermore, the positive effects of additional agricultural practices on the nutrient regulation ES demand were identified. The spatial distribution of the nutrient regulation ES demand was compared to the distribution of the nitrate leaching and denitrification potential in order to analyse the relative vulnerability of individual fields to nutrient oversupply. The findings of this study, which highlight in particular the suitability of enlarged crop rotation systems, can be used to support sustainable agricultural practices and land management strategies on the local sale. EDITED BY Christine Fürst.