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Blue native DIGE as a tool for comparative analyses of protein complexes

Heinemeyer, Jesco; Scheibe, Burghardt; Schmitz, Udo K.; Braun, Hans-Peter (New York, NY [u.a.] : Elsevier, 2009)

Differential gel electrophoresis (DIGE) is based on pre-labeling of different protein fractions and their subsequent co-electrophoresis in a single gel. Cyanine based "CyDye DIGE Fluor minimal dyes" are used for the labeling ...
L-galactono-1,4-lactone dehydrogenase (GLDH) forms part of three subcomplexes of mitochondrial complex I in Arabidopsis thaliana

Schertl, Peter; Sunderhaus, Stephanie; Klodmann, Jennifer; Gergoff Grozeff, Gustavo E.; Bartoli, Carlos G.; Braun, Hans-Peter (Bethesda, Md. : ASBMB Publications, 2012)

L-Galactono-1,4-lactone dehydrogenase (GLDH) catalyzes the terminal step of the Smirnoff-Wheeler pathway for vitamin C (L-ascorbate) biosynthesis in plants. A GLDH in gel activity assay was developed to biochemically ...
New insights into the co-evolution of cytochrome c reductase and the mitochondrial processing peptidase

Brumme, Stefanie; Kruft, Volker; Schmitz, Udo K.; Braun, Hans-Peter (Bethesda, Md. : ASBMB Publications, 1998)

The mitochondrial processing peptidase (MPP) is a heterodimeric enzyme that forms part of the cytochrome c reductase complex from higher plants. Mitochondria from mammals and yeast contain two homologous enzymes: (i) an ...
Single-use membrane adsorbers for endotoxin removal and purification of endogenous polysialic acid from Escherichia coli K1

de Vries, Ingo; Schreiber, Sarah; Boßmann, Daniel; Hellmann, Zawadi; Kopatz, Jens; Neumann, Harald; Beutel, Sascha (Amsterdam : Elsevier B.V., 2018)

Polysialic acid (polySia) is a promising molecule for various medical applications (e.g., treatment of inflammatory neurodegenerative diseases). In this study a complete production process for human-identical α-(2,8)-linked ...
SUMO-fusion, purification, and characterization of a (+)-zizaene synthase from Chrysopogon zizanioides

Hartwig, Steffen; Frister, Thore; Alemdar, Semra; Li, Zhaopeng; Scheper, Thomas; Beutel, Sascha (Cambridge, MA : Elsevier Academic Press, 2015)

An uncharacterized plant cDNA coding for a polypeptide presumably having sesquiterpene synthase activity, was expressed in soluble and active form. Two expression strategies were evaluated in Escherichia coli. The enzyme ...
Supramolecular structure of the mitochondrial oxidative phosphorylation system

Boekema, Egbert J.; Braun, Hans-Peter (Bethesda, Md. : ASBMB Publications, 2007)

The protein complexes of the mitochondrial oxidative phosphorylation system were recently reported to form supramolecular assemblies termed respiratory supercomplexes or respirasomes. These supercomplexes are considered ...

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Browsing by Subject "gel electrophoresis"

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