2024-03-28T13:00:03Zhttps://www.repo.uni-hannover.de/oai/requestoai:www.repo.uni-hannover.de:123456789/412022-12-02T15:02:16Zcom_123456789_1col_123456789_2ddc:550doc-type:Articledoc-type:Textopen_accessddc:590status-type:publishedVersionddc:570
Sybertz, Janine
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600
Reich, Michael
aa6f290b-6a4b-4ae0-84f8-fa4c0bd65395
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2015-08-03T13:49:17Z
2015-08-03T13:49:17Z
2015
Sybertz, Janine; Reich, Michael: Assessing Climate Change Induced Turnover in Bird Communities Using Climatically Analogous Regions. In: Diversity 7 (2015), Nr. 1, S. 36-59. DOI: http://dx.doi.org/10.3390/d7010036
http://www.repo.uni-hannover.de/handle/123456789/41
http://dx.doi.org/10.15488/23
It is crucial to define and quantify possible impacts of climate change on wildlife in order to be able to pre-adapt management strategies for nature conservation. Thus, it is necessary to assess which species might be affected by climatic changes, especially at the regional scale. We present a novel approach to estimate possible climate change induced turnovers in bird communities and apply this method to Lüneburg Heath, a region in northern Germany. By comparing species pools of future climatically analogous regions situated in France with the Lüneburg Heath species pool, we detected possible trends for alterations within the regional bird community in the course of climate change. These analyses showed that the majority of bird species in Lüneburg Heath will probably be able to tolerate the projected future climate conditions, but that bird species richness, in general, may decline. Species that might leave the community were often significantly associated with inland wetland habitats, but the proportion of inland wetlands within the regions had a significant influence on the magnitude of this effect. Our results suggest that conservation efforts in wetlands have to be strengthened in light of climate change because many species are, in principle, able to tolerate future climate conditions if sufficient habitat is available.
Made available in DSpace on 2015-08-03T13:49:17Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
DFG
Ministry for Science and Culture of Lower Saxony/KLIFF
publishedVersion
eng
Basel : MDPI AG
Diversity 7 (2015), Nr. 1
1424-2818
http://dx.doi.org/10.3390/d7010036
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
breeding birds
analogous climates
climate change projections
nature conservation
Lüneburg Heath
Lüneburger Heide
Brutvögel
Klimawandel
Klimaänderung
Brutvögel
Lüneburger Heide
Dewey Decimal Classification::500 | Naturwissenschaften::550 | Geowissenschaften
550
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::590 | Tiere (Zoologie)
590
600
Assessing Climate Change Induced Turnover in Bird Communities Using Climatically Analogous Regions
Article
Text
36
59
openAccess
Publikation wurde durch den Publikationsfonds gefördert.
LUH_Fonds
ORIGINAL
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oai:www.repo.uni-hannover.de:123456789/422022-12-02T16:11:41Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Taubert, Johannes
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600
Hou, Bo
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600
Risselada, H. Jelger
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600
Mehner, Denise
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600
Lünsdorf, Heinrich
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600
Grubmüller, Helmut
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Brüser, Thomas
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2015-08-03T15:43:17Z
2015-08-03T15:43:17Z
2015
Taubert, Johannes; Hou, Bo; Risselada, H. Jelger; Mehner, Denise; Lünsdorf, Heinrich; Grubmüller, Helmut; Brüser, Thomas: TatBC-Independent TatA/Tat Substrate Interactions Contribute to Transport Efficiency. In: PLOS ONE 10 (2015), Nr. 3. DOI: http://dx.doi.org/10.1371/journal.pone.0119761
http://www.repo.uni-hannover.de/handle/123456789/42
http://dx.doi.org/10.15488/24
The Tat system can transport folded, signal peptide-containing proteins (Tat substrates) across energized membranes of prokaryotes and plant plastids. A twin-arginine motif in the signal peptide of Tat substrates is recognized by TatC-containing complexes, and TatA permits the membrane passage. Often, as in the model Tat systems of Escherichia coli and plant plastids, a third component-TatB-is involved that resembles TatA but has a higher affinity to TatC. It is not known why most TatA dissociates from TatBC complexes in vivo and distributes more evenly in the membrane. Here we show a TatBC-independent substrate-binding to TatA from Escherichia coli, and we provide evidence that this binding enhances Tat transport. First hints came from in vivo cross-linking data, which could be confirmed by affinity co-purification of TatA with the natural Tat substrates HiPIP and NrfC. Two positions on the surface of HiPIP could be identified that are important for the TatA interaction and transport efficiency, indicating physiological relevance of the interaction. Distributed TatA thus may serve to accompany membrane-interacting Tat substrates to the few TatBC spots in the cells.
Made available in DSpace on 2015-08-03T15:43:17Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
DFG/GRK/1026
publishedVersion
eng
San Francisco : Public Library of Science
PLoS ONE 10 (2015), Nr. 3
1932-6203
http://dx.doi.org/10.1371/journal.pone.0119761
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
twin-arginine translocation
dependent protein-transport
coli plasma-membrane
Escherichia-coli
signal peptide
Streptomyces-lividans
pathway specificit
precursor proteins
Bacillus-subtilis
binding-site
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
TatBC-Independent TatA/Tat Substrate Interactions Contribute to Transport Efficiency
Article
Text
openAccess
LUH_Fonds
ORIGINAL
journal.pone.0119761.pdf
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oai:www.repo.uni-hannover.de:123456789/42
2022-12-02 17:11:41.348
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/462022-12-02T16:10:15Zcom_123456789_1col_123456789_8ddc:550doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Gentsch, Norman
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600
Mikutta, Robert
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600
Alves, Ricardo J. Eloy
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600
Barta, Jin
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600
Čapek, Petr
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600
Gittel, Antje
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600
Hugelius, Gustaf
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600
Kuhry, Peter
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600
Lashchinskiy, Nikolay
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600
Palmtag, Juri
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600
Richter, Andreas
52d71f9c-5e93-47ae-9872-02fab3c97c0c
600
Šantrůčková, Hana
2ed723f8-fdb2-4d46-891c-99dc5e4401e6
600
Schnecker, Jörg
bbdff247-031d-4761-be64-b301808b0a5b
600
Shibistova, Olga
15b92bb6-8e1a-4c95-bbb7-52355b5e44a6
600
Urich, Tim
4a5959b2-6d53-4737-929c-5fca617fe751
600
Wild, B.
5215a0ee-0a00-4cfc-aa4c-18017d9e795b
600
Guggenberger, Georg
32226bc2-d1b6-49d7-bae6-fda855a5f5d4
600
2015-08-06T12:32:33Z
2015-08-06T12:32:33Z
2015
Gentsch, N.; Mikutta, R.; Alves, R.J.E.; Barta, J.; Čapek, P.; Gittel, A. et al.: Storage and transformation of organic matter fractions in cryoturbated permafrost soils across the Siberian Arctic. In: Biogeosciences 12 (2015), S. 4525-4542. DOI: http://dx.doi.org/10.5194/bg-12-4525-2015
http://www.repo.uni-hannover.de/handle/123456789/46
http://dx.doi.org/10.15488/28
In permafrost soils, the temperature regime and the resulting cryogenic processes are important determinants of the storage of organic carbon (OC) and its small-scale spatial variability. For cryoturbated soils, there is a lack of research assessing pedon-scale heterogeneity in OC stocks and the transformation of functionally different organic matter (OM) fractions, such as particulate and mineral-associated OM. Therefore, pedons of 28 Turbels were sampled in 5 m wide soil trenches across the Siberian Arctic to calculate OC and total nitrogen (TN) stocks based on digital profile mapping. Density fractionation of soil samples was performed to distinguish between particulate OM (light fraction, LF, < 1.6 g cm−3), mineral associated OM (heavy fraction, HF, > 1.6 g cm−3), and a mobilizable dissolved pool (mobilizable fraction, MoF). Across all investigated soil profiles, the total OC storage was 20.2 ± 8.0 kg m−2 (mean ± SD) to 100 cm soil depth. Fifty-four percent of this OC was located in the horizons of the active layer (annual summer thawing layer), showing evidence of cryoturbation, and another 35 % was present in the upper permafrost. The HF-OC dominated the overall OC stocks (55 %), followed by LF-OC (19 % in mineral and 13 % in organic horizons). During fractionation, approximately 13 % of the OC was released as MoF, which likely represents a readily bioavailable OM pool. Cryogenic activity in combination with cold and wet conditions was the principle mechanism through which large OC stocks were sequestered in the subsoil (16.4 ± 8.1 kg m−2; all mineral B, C, and permafrost horizons). Approximately 22 % of the subsoil OC stock can be attributed to LF material subducted by cryoturbation, whereas migration of soluble OM along freezing gradients appeared to be the principle source of the dominant HF (63 %) in the subsoil. Despite the unfavourable abiotic conditions, low C / N ratios and high δ13C values indicated substantial microbial OM transformation in the subsoil, but this was not reflected in altered LF and HF pool sizes. Partial least-squares regression analyses suggest that OC accumulates in the HF fraction due to co-precipitation with multivalent cations (Al, Fe) and association with poorly crystalline iron oxides and clay minerals. Our data show that, across all permafrost pedons, the mineral-associated OM represents the dominant OM fraction, suggesting that the HF-OC is the OM pool in permafrost soils on which changing soil conditions will have the largest impact.
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bg-12-4525-2015.pdf: 3363655 bytes, checksum: 2102950758dd923fa47262bccdc6924f (MD5)
Previous issue date: 2015
Russian Ministry of Education and Science/14.B25.31.0031
German Federal Ministry of Education and Research/03F0616A
Evangelisches Studienwerk Villigst
DFG
publishedVersion
eng
Göttingen : Copernicus
CC BY 3.0 Unported
http://creativecommons.org/licenses/by/3.0/
permafrost soils
organic carbon
carbon
siberian arctic
gelogy
biology
arctic
siberia
Geologie
Biologie
Permafrostboden
Kohlenstoff
organischer Kohlenstoff
Arktis
Sibirien
Geologie
Biologie
Dauerfrostboden
Kohlenstoff
Arktis
Sibirien
Dewey Decimal Classification::500 | Naturwissenschaften::550 | Geowissenschaften
550
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Storage and transformation of organic matter fractions in cryoturbated permafrost soils across the Siberian Arctic
Article
Text
1726-4189
1726-4170
http://dx.doi.org/10.5194/bg-12-4525-2015
4525
4542
openAccess
Publikation wurde durch den Publikationsfonds gefördert.
Biogeosciences 12 (2015), S. 4525-4542
Norman
Gentsch
Mikutta
Alves
Barta
Čapek
Gittel
Hugelius
Kuhry
Lashchinskiy
Palmtag
Richter
Šantrůčková
Schnecker
Shibistova
Urich
Wild
Guggenberger
LUH_Fonds
ORIGINAL
bg-12-4525-2015.pdf
bg-12-4525-2015.pdf
application/pdf
3363655
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Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
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Neunaber, Janek
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Stahl, Frank
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Walter, Johanna-Gabriela
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Scheper, Thomas
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Eichner, Simone
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Kirschning, Andreas
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Zeilinger, Carsten
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2015-10-14T08:29:50Z
2015-10-14T08:29:50Z
2014-12-22
Schax, Emilia; Neunaber, Janek; Stahl, Frank; Walter, Johanna-Gabriela; Scheper, Thomas; Eichner, Simone et al.: Multiplexed heat shock protein microarray as a screening platform for the selection of novel drug compounds. In: Biodiscovery 14 (2014), Nr. 1. DOI: http://dx.doi.org/10.7750/BioDiscovery.2014.14.1
http://www.repo.uni-hannover.de/handle/123456789/76
http://dx.doi.org/10.15488/58
In diseases such as cancer, Alzheimer’s disease or malaria, disease-related proteins take advantage of the heat shock protein (HSP) control system for their own activation or maturation. There is a quest to find inhibitors that specifically bind to the HSPs. Here, we report on a novel multiplexed assay system for inhibitor screening based on a protein microarray (MA) technique that was developed for routine applications with storable MAs. Purified HSPs are printed as full-length proteins on microarrays and used as a drug target for the screening of new inhibitors. Derivatives obtained by a combination of biological and chemical synthesis were tested as competitors of ATP with a suggested affinity for several HSP proteins which are hHSP from human, AtHSP83 (Arabidopsis thaliana) and HtpG from Helicobacter pylori. Some of these new derivatives exerted selectivity between human and bacterial heat shock proteins. Printed human HSP90 was used to test the binding of denatured proteins on the client binding site of human HSP90, since the full-length HSP maintains the capability to bind putative clients or cochaperones. Initial data revealed that the microarray application can be used to identify directly elevated heat-shock protein levels in cancer cell lysates. We suggest that microarray-based assaying of HSP levels can be used as a marker for determining stress levels.
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Previous issue date: 2014-12-22
DFG/Ki 13-1
publishedVersion
eng
Dundee : Dundee Science Press Ltd
Biodiscovery (2014), Nr. 14
2050-2966
http://dx.doi.org/10.7750/BioDiscovery.2014.14.1
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
heat shock protein
inhibitor
non benzoquinone geldanamycin
protein microarray
screening
Hitzeschockproteine
Inhibitor
Nicht-Benzochinon-Geldanamycin
Protein-Mikroarray
Protein-Chip
Screening
Hitzeschock-Proteine
Inhibitor
Microarray
Screening
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
540
600
Multiplexed heat shock protein microarray as a screening platform for the selection of novel drug compounds
Article
Text
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Publikation wurde durch den Publikationsfonds gefördert.
Biodiscovery (2014), Nr. 14
Emilia
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Thomas
Simone
Andreas
Carsten
Schax
Neunaber
Stahl
Walter
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Zeilinger
LUH_Fonds
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2022-12-02 17:16:26.782
Institutionelles Repositorium der Leibniz Universität Hannover
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Lesker, Till
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Maiss, Edgar
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2015-10-13T14:36:04Z
2013-10-09
Lesker, Till; Maiss, Edgar: In planta Protein Interactions of Three Alphacryptoviruses and Three Betacryptoviruses from White Clover, Red Clover and Dill by Bimolecular Fluorescence Complementation Analysis. In: Viruses 5 (2013), Nr. 10, S. 2512-2530. DOI: http://dx.doi.org/10.3390/v5102512
http://www.repo.uni-hannover.de/handle/123456789/74
http://dx.doi.org/10.15488/56
Plant-infecting viruses of the genera Alpha- and Betacryptovirus within the family Partitiviridae cause no visible effects on their hosts and are only transmitted by cell division and through gametes. The bipartite dsRNA genome is encoding a RNA-dependent RNA polymerase (RdRp) and a coat protein (CP). Aside from sequence and structural analysis, the investigation of protein interactions is another step towards virus characterization. Therefore, ORFs of two type members White Clover Cryptic Virus 1 and 2 (WCCV-1 and WCCV-2), as well as the related viruses from Red Clover and Dill were introduced into a bimolecular fluorescence complementation assay. We showed CP-CP dimerization for all tested viruses with localization for alphacryptoviruses at the nuclear membrane and for betacryptoviruses close to cell walls within the cytoplasm. For CPs of WCCV-1 and WCCV-2, deletion mutants were created to determine internal interaction sites. Moreover, RdRp self-interaction was found for all viruses, whereas CP-RdRp interactions were only detectable for the alphacryptoviruses. An intra-genus test of CPs was successful in various virus combinations, whereas an inter-genus interaction of WCCV-1CP and WCCV-2CP was absent. This is the first report of in vivo protein interactions of members in the family Partitiviridae, indicating distinct features of the alpha- and betacryptoviruses.
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Previous issue date: 2013-10-09
DFG
publishedVersion
eng
Basel : MDPI AG
Viruses 5 (2013), Nr. 10
1999-4915
http://dx.doi.org/10.3390/v5102512
CC BY 3.0 Unported
http://creativecommons.org/licenses/by/3.0/
Partitiviridae
Alphacryptovirus
Betacryptovirus
protein interaction
Bimolecular fluorescence complementation
BiFC
White Clover
Red Clover
Dill
Partitiviridae
Alphacryptovirus
Betacryptovirus
Protein-Wechselwirkung
Bimolekulare Fluoreszenzkomplementation
BiFC
Trifolium repens
Trifolium pratense
Anethum graveolens
Pflanzenviren
Protein-Protein-Wechselwirkung
Weißklee
Rotklee
Dill
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
580
600
In planta Protein Interactions of Three Alphacryptoviruses and Three Betacryptoviruses from White Clover, Red Clover and Dill by Bimolecular Fluorescence Complementation Analysis
Article
Text
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2530
openAccess
Publikation wurde durch den Publikationsfonds gefördert.
Viruses 5 (2013), Nr. 10
Till
Edgar
Lesker
Maiss
LUH_Fonds
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2022-12-02 17:10:14.743
Institutionelles Repositorium der Leibniz Universität Hannover
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Zeilinger, Carsten
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2015-10-14T09:19:16Z
2015-10-14T09:19:16Z
2014
Zeilinger, Carsten: MJK2, a K+Channel from M. Jannaschii Mediates pH Dependent Potassium Transport Activity. In: Journal of Physical Chemistry & Biophysics 4 (2014), Nr. 5. DOI: dx.doi.org/10.4172/2161-0398.1000156
http://www.repo.uni-hannover.de/handle/123456789/77
http://dx.doi.org/10.15488/59
MjK2 was expressed in E. coli cells as a fusion protein containing N-or C-terminal an antibody binding site and a histidinehexamer. The C-terminal tagged fusion protein allows the expression and purification of an extra soluble RCK domain at p34 kDa, whereas this additional RCK domain was lost when the N-terminal tagged construct was used. Upon removal of the fusion peptide from the purified N-terminal tagged channel monomer, MjK2 occurred as a stable tetramer when incubated with synthetic lipid. The channel activity was studied after reconstitution into liposomes by single channel recording or by an optical assay with the potassium sensing dye, PBFI. First the channel function was improved by single channel recording. Single channel recording confirmed the pH dependence of the channel activity with single channel conductances of 42, 70, 85 and 202 pS and indicated that a functional K+ channel was formed. To study the function of the reconstituted MjK2 activity in an optical assay the potassium release was initiated when the external BaCl2 block was compensated by addition of EDTA. The release of potassium was mediated by reconstituted MjK2 at low pH or by the presence of internal calcium at high pH. MgCl2 had no or weak effect, whereas cAMP at low pH caused a complete loss of potassium during the preparation. Alignments studies revealed that MjK2 has different structural features in the channel pore and the RCK composition and therefore a different function can be expected. Amino acid sequence and structural alignments showed that a Ca2+ binding site and a typical nucleotide-binding site is not present in the RCK domain of MjK2 and therefore a different behavior could be expected. In addition a lysine reach linker region as found in human sperm K+ channels hslo1 and hslo3can play similar role in the gating behavior.
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Previous issue date: 2014
Leibniz University Hannover/WiF program
DFG
publishedVersion
eng
OMICS Pub. Group : Sunnyvale, Calif.
Journal of Physical Chemistry & Biophysics 4 (2014), Nr. 5
2161-0398
http://dx.doi.org/10.4172/2161-0398.1000156
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
MjK2
K+ channel 2 of M. jannaschii
RCK
Regulator of Conductance of Potassium
Reconstitution
Single channel recording
PBFI
Benzofuranisophthalate dye
Methanococcus jannaschii
Methanocaldococcus jannaschii
MjK2
Methanocaldococcus jannaschii
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
MJK2, a K+Channel from M. Jannaschii Mediates pH Dependent Potassium Transport Activity
Article
Text
openAccess
Publikation wurde durch den Publikationsfonds gefördert.
Journal of Physical Chemistry & Biophysics 4 (2014), Nr. 5
Carsten
Zeilinger
LUH_Fonds
ORIGINAL
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2022-12-02 17:11:41.352
Institutionelles Repositorium der Leibniz Universität Hannover
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Yim, Bunlong
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Winkelmann, Traud
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Ding, Guo-Chun
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Smalla, Kornelia
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2015-11-06T11:03:59Z
2015-11-06T11:03:59Z
2015-11-06
Yim, Bunlong; Winkelmann, Traud; Ding, Guo-Chun; Smalla, Kornelia: Different bacterial communities in heat and gamma irradiation treated replant disease soils revealed by 16S rRNA gene analysis – contribution to improved aboveground apple plant growth? In: Frontiers in Microbiology 6 (2015), Nr. 8. DOI: http://dx.doi.org/10.3389/fmicb.2015.01224
http://www.repo.uni-hannover.de/handle/123456789/120
http://dx.doi.org/10.15488/102
Replant disease (RD) severely affects apple production in propagation tree nurseries and in fruit orchards worldwide. This study aimed to investigate the effects of soil disinfection treatments on plant growth and health in a biotest in two different RD soil types under greenhouse conditions and to link the plant growth status with the bacterial community composition at the time of plant sampling. In the biotest performed we observed that the aboveground growth of apple rootstock M26 plants after 8 weeks was improved in the two RD soils either treated at 50°C or with gamma irradiation compared to the untreated RD soils. Total community DNA was extracted from soil loosely adhering to the roots and quantitative real-time PCR revealed no pronounced differences in 16S rRNA gene copy numbers. 16S rRNA gene-based bacterial community analysis by denaturing gradient gel electrophoresis (DGGE) and 454-pyrosequencing revealed significant differences in the bacterial community composition even after 8 weeks of plant growth. In both soils, the treatments affected different phyla but only the relative abundance of Acidobacteria was reduced by both treatments. The genera Streptomyces, Bacillus, Paenibacillus, and Sphingomonas had a higher relative abundance in both heat treated soils, whereas the relative abundance of Mucilaginibacter, Devosia, and Rhodanobacter was increased in the gamma-irradiated soils and only the genus Phenylobacterium was increased in both treatments. The increased abundance of genera with potentially beneficial bacteria, i.e., potential degraders of phenolic compounds might have contributed to the improved plant growth in both treatments.
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Previous issue date: 2015-11-06
BÖLN
DFG
publishedVersion
eng
Lausanne : Frontiers Research Foundation
Frontiers in Microbiology 6 (2015), Nr. 8
1664-302X
http://dx.doi.org/10.3389/fmicb.2015.01224
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
biotest
apple replant disease
DGGE
qPCR
pyrosequencing
bacterial community composition
bacterial diversity
Biotest
Apfel
Nachbaukrankheit
DGGE
Denaturierungsgradientengelelektrophorese
qPCR
quantitative Echtzeit PCR
Quantitative Real-Time PCR
Pyrosequenzierung
Bakteriengemeinschaft
Zusammensetzung
bakterielle Vielfalt
Vielfalt
Bakterien
Biotest
Apfel
Apfelanbau
Apfelkrankheit
Specific apple replant disease
Denaturierende Gradienten-Gelelektrophorese
Real time quantitative PCR
Sequenzanalyse <Chemie>
Bakterien
Vielfalt
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
580
600
Different bacterial communities in heat and gamma irradiation treated replant disease soils revealed by 16S rRNA gene analysis – contribution to improved aboveground apple plant growth?
Article
Text
openAccess
Publikation wurde durch den Publikationsfonds der Leibniz Universität Hannover gefördert.
Frontiers in Microbiology 6 (2015), Nr. 8
Bunlong
Traud
Guo-Chun
Kornelia
Yim
Winkelmann
Ding
Smalla
LUH_Fonds
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2022-12-02 17:11:41.392
Institutionelles Repositorium der Leibniz Universität Hannover
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Cox, Russell J.
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Cox, Russell: Oxidative rearrangements during fungal biosynthesis. In: Natural Product Reports 31 (2014), Nr. 10, S. 1405-1424. DOI: http://dx.doi.org/10.1039/c4np00059e
http://www.repo.uni-hannover.de/handle/123456789/123
http://dx.doi.org/10.15488/105
Oxidative rearrangements are key reactions during the biosyntheses of many secondary metabolites in fungi. This review highlights the most important examples of these reactions and aims to draw together key mechanistic themes to allow a better understanding and future exploitation of this key class of fungal catalysts.
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Previous issue date: 2014-07-25
publishedVersion
eng
Cambridge : Royal Society of Chemistry
Natural Product Reports 31 (2014), Nr. 10
1460-4752
0265-0568
http://dx.doi.org/10.1039/c4np00059e
CC BY-NC 3.0 Unported
http://creativecommons.org/licenses/by-nc/3.0/
cephalosporin-c biosynthesis
nuclear-magnetic-resonance
cell-free-extracts
aflatoxin biosynthesis
ring-expansion
aspergillus-nidulans
penicillin-n
deacetoxycephalosporin-c
heterologous expression
polyketide biosynthesis
Cephalosporine
Cephalosporine C
Biosynthese
Cephalosporine C-Biosynthese
Kernmagnetische Resonanz
Kernspinresonanz
zellfreier Extrakt
Rohextrakt
Aflatoxin
Aflatoxin-Biosynthese
Ringerweiterung
Aspergillus nidulans
Gießkannenschimmel
Penicillin N
Desacetoxycephalosporin C
Heterologe Expression
Polyketid
Polyketid-Biosynthese
Cephalosporin C
Biosynthese
Magnetische Kernresonanz
Extrakt
Aflatoxin
Ringerweiterung <Chemie>
Aspergillus nidulans
Penicilline
Heterologe Genexpression
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
540
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Oxidative rearrangements during fungal biosynthesis
Article
Text
1405
1424
openAccess
Publikation wurde nicht durch einen RSC "Gold for Gold" Gutschein der TIB/UB gefördert.
Natural Product Reports 31 (2014), Nr. 10, S. 1405-1424
Russell
Cox
ORIGINAL
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Institutionelles Repositorium der Leibniz Universität Hannover
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oai:www.repo.uni-hannover.de:123456789/1352022-12-13T15:13:29Zcom_123456789_1col_123456789_8doc-type:Articleddc:540doc-type:Textopen_accessstatus-type:publishedVersionddc:570
Geist, Egor
fb2661a9-7320-4634-814a-39c845d2a123
600
Kirschning, Andreas
5dc1019e-8046-445e-9baa-8aea250fdb95
600
Schmidt, Thomas
5a5f90c9-4412-480a-b2d7-12a95b2d17b0
600
2015-11-19T13:00:00Z
2015-11-19T13:00:00Z
2014-02-27
Geist, Egor; Kirschning, Andreas; Schmidt, Thomas: sp(3)-sp(3) Coupling reactions in the synthesis of natural products and biologically active molecules. In: Natural Product Reports 31 (2014), Nr. 4, S. 441-448. DOI: http://dx.doi.org/10.1039/c3np70108e
http://www.repo.uni-hannover.de/handle/123456789/135
http://dx.doi.org/10.15488/117
This Highlight covers the current status of relatively unexplored sp(3)-sp(3) cross-coupling reactions with particular focus on natural product and related syntheses.
Made available in DSpace on 2015-11-19T13:00:00Z (GMT). No. of bitstreams: 0
Previous issue date: 2014-02-27
publishedVersion
eng
Cambridge : Royal Society of Chemistry
Natural Product Reports 31 (2014), Nr. 4
1460-4752
0265-0568
http://dx.doi.org/10.1039/c3np70108e
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
Negishi Cross-Couplings
Secondary Alkyl-Halides
Sp(3) Carbon Centers
Room-Temperature
Sorangium-Cellulosum
Grignard-Reagents
Electrophiles
Acetogenins
Activation
Complexes
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
540
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
sp(3)-sp(3) Coupling reactions in the synthesis of natural products and biologically active molecules
Article
Text
4
31
441
448
openAccess
Nationallizenz
ORIGINAL
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123456789/135
oai:www.repo.uni-hannover.de:123456789/135
2022-12-13 16:13:29.349
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/1362022-12-13T15:13:29Zcom_123456789_1col_123456789_8doc-type:Articleddc:540doc-type:Textopen_accessstatus-type:publishedVersionddc:570
Kalesse, Markus
8f2d4d6a-2c6b-42e6-86e5-bd96bf4cd67f
600
Cordes, Martin
c0618b37-8686-42b9-a113-28fc3ac9108d
600
Symkenberg, Gerrit
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600
Lu, Hai-Hua
208eeaaa-e2bf-4ee2-91af-1b287d9e3420
600
2015-11-19T13:00:01Z
2015-11-19T13:00:01Z
2014-03-05
Kalesse, Markus; Cordes, Martin; Symkenberg, Gerrit; Lu, Hai-Hua: The vinylogous Mukaiyama aldol reaction (VMAR) in natural product synthesis. In: Natural Product Reports 31 (2014), Nr. 4, S. 563-594. DOI: http://dx.doi.org/10.1039/c3np70102f
http://www.repo.uni-hannover.de/handle/123456789/136
http://dx.doi.org/10.15488/118
The vinylogous Mukaiyama aldol reaction (VMAR) allows efficient access to larger segments for complex natural product synthesis, primarily polyketides, through the construction of vicinal hydroxyl and methyl groups as well as di and tri-substituted double bonds in one single operation. In this review, we will highlight stereoselective protocols that have been used in natural product synthesis and cluster them into the four groups that can be obtained from different silyl ketene acetals or enol ethers. At the beginning, an overview on different stereoselective VMARs is presented; disregarding their applications in total syntheses.
Made available in DSpace on 2015-11-19T13:00:01Z (GMT). No. of bitstreams: 0
Previous issue date: 2014-03-05
publishedVersion
eng
Cambridge : Royal Society of Chemistry
Natural Product Reports 31 (2014), Nr. 4
1460-4752
0265-0568
http://dx.doi.org/10.1039/c3np70102f
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
1st Total-Synthesis
Synthesis
Alga Constantinea-Simplex
Lewis-Base Activation
Stereocontrolled Total-Synthesis
Enantioselective Total-Synthesis
Silyl Ketene Acetals
Sponge Tedania-Ignis
Addition-Reactions
Marine Sponge
Scytophycin-C
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
540
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
The vinylogous Mukaiyama aldol reaction (VMAR) in natural product synthesis
Article
Text
4
31
563
594
openAccess
Nationallizenz
ORIGINAL
c3np70102f.pdf
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application/pdf
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https://www.repo.uni-hannover.de/bitstream/123456789/136/1/c3np70102f.pdf
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123456789/136
oai:www.repo.uni-hannover.de:123456789/136
2022-12-13 16:13:29.357
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/1582022-12-13T15:13:29Zcom_123456789_1col_123456789_8doc-type:Articleddc:540doc-type:Textopen_accessstatus-type:publishedVersionddc:570
Juerjens, Gerrit
7c6ab3f0-95d6-480c-ae2c-27654eb10d6a
600
Kirschning, Andreas
5dc1019e-8046-445e-9baa-8aea250fdb95
600
Candito, David A.
45c6802a-cc04-4145-b7f4-1aafe0cebde3
600
2015-12-08T13:08:05Z
2016-03-02T23:05:29Z
2015-03-02
Juerjens, Gerrit; Kirschning, Andreas; Candito, David A.: Lessons from the Synthetic Chemist Nature. In: Natural Product Reports 32 (2015), Nr. 5, S. 723-737. DOI: http://dx.doi.org/10.1039/c4np00160e
http://www.repo.uni-hannover.de/handle/123456789/158
http://dx.doi.org/10.15488/140
This conceptual review examines the ideal multistep synthesis from the perspective of nature. We suggest that besides step- and redox economies, one other key to efficiency is steady state processing with intermediates that are immediately transformed to the next intermediate when formed. We discuss four of nature's strategies (multicatalysis, domino reactions, iteration and compartmentation) that commonly proceed via short-lived intermediates and show that these strategies are also part of the chemist's portfolio. We particularly focus on compartmentation which in nature is found microscopically within cells (organelles) and between cells and on a molecular level on multiprotein scaffolds (e.g. in polyketide synthases) and demonstrate how compartmentation is manifested in modern multistep flow synthesis.
Made available in DSpace on 2015-12-08T13:08:05Z (GMT). No. of bitstreams: 0
Previous issue date: 2015-03-02
publishedVersion
eng
Cambridge : Royal Society of Chemistry
Natural Product Reports 32 (2015), Nr. 5
1460-4752
0265-0568
http://dx.doi.org/10.1039/c4np00160e
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
asymmetric total-synthesis
one-pot preparation
organic-synthesis
isotactic polymethoxy-1-alkenes
enantioselective hydrogenation
polyketide synthases
product synthesis
ideal synthesis
step economy
biosynthesis
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
540
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Lessons from the Synthetic Chemist Nature
Article
Text
5
32
723
737
openAccess
2016-03-02
Nationallizenz
ORIGINAL
c4np00160e.pdf
c4np00160e.pdf
application/pdf
2058006
https://www.repo.uni-hannover.de/bitstream/123456789/158/1/c4np00160e.pdf
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oai:www.repo.uni-hannover.de:123456789/158
2022-12-13 16:13:29.392
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/2132022-12-13T15:12:26Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Breidert, Stephanie
e9c1023d-c73d-4883-8bb7-0fbdcfecf680
600
Jacob, Ralf
2f6dc730-91c9-48c4-9a1e-0c090ceb1833
600
Ngezahayo, Anaclet
98e403d3-05d2-4c08-a36f-2a82d0a12bc6
600
Kolb, Hans-Albert
f4cb93e7-f7aa-4727-8a17-cc97721f2cea
600
Naim, Hassan Y.
134ba263-fe10-4c41-a5b2-64b1187d0038
600
2016-02-02T12:03:12Z
2016-02-02T12:03:12Z
2005-06-01
Breidert, S.; Jacob, R.; Ngezahayo, A.; Kolb, H.A.; Naim, H.Y.: Trafficking pathways of Cx49-GFP in living mammalian cells. In: Biological Chemistry 386 (2005), Nr. 2, S. 155-160. DOI: http://dx.doi.org/10.1515/BC.2005.019
http://www.repo.uni-hannover.de/handle/123456789/213
http://dx.doi.org/10.15488/191
In the present study we examined the trafficking pathways of connexin49 (Cx49) fused to green fluorescent protein (GFP) in polar and non-polar cell lines. The Cx49 gene was isolated from ovine lens by RT-PCR. Cx49 cDNA was fused to GFP and the hybrid cDNA was transfected into several cell lines. After transfection of Cx49-GFP cDNA into HeLa cells, it was shown using the double whole-cell patch-clamp technique that the expressed fusion protein was still able to form conducting gap junction channels. Synthesis, assembly, and turnover of the Cx49-GFP hybrid protein were investigated using a pulse-chase protocol. A major 78-kDa protein band corresponding to Cx49-GFP could be detected with a turnover of 16-20 h and a half-life time of 10 h. The trafficking pathways of Cx49-GFP were monitored by confocal laser microscopy. Fusion proteins were localized in subcellular compartments, including the endoplasmic reticulum (ER), the ER-Golgi intermediate compartment, the Golgi apparatus, and the trans-Golgi network, as well as vesicles traveling towards the plasma membrane. Time-dependent sequential localization of Cx49-GFP in the ER and then the Golgi apparatus supports the notion of a slow turnover of Cx49-GFP compared to other connexins analyzed so far. Gap junction plaques resembling the usual punctuate distribution pattern could be demonstrated for COS-1 and MDCK cells. Basolateral distribution of Cx49-GFP was observed in polar MDCK cells, indicating specific sorting behavior of Cx49 in polarized cells. Together, this report describes the first characterization of biosynthesis and trafficking of lens Cx49.
Made available in DSpace on 2016-02-02T12:03:12Z (GMT). No. of bitstreams: 0
Previous issue date: 2005-06-01
Fritz Thyssen-Stiftung
publishedVersion
eng
Berlin : Walter de Gruyter
Biological Chemistry 386 (2005), Nr. 2
1437-4315
1431-6730
http://dx.doi.org/10.1515/BC.2005.019
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
connexin49
green fluorescent protein
mammalian cells
protein assembly
protein transport
pulse-chase experiments
gap-junction channels
green fluorescent protein
hela-cells
molecular-cloning
connexin
lens
phosphorylation
permeability
degradation
cortex
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Trafficking pathways of Cx49-GFP in living mammalian cells
Article
Text
2
386
155
160
openAccess
Nationallizenz
ORIGINAL
bc.2005.019.pdf
bc.2005.019.pdf
application/pdf
123332
https://www.repo.uni-hannover.de/bitstream/123456789/213/1/bc.2005.019.pdf
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123456789/213
oai:www.repo.uni-hannover.de:123456789/213
2022-12-13 16:12:26.543
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/2592022-12-13T15:12:26Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:580ddc:570
Zorn, Holger
bbf09d10-e7b6-4606-b1ec-257f9a1e82d2
600
Bouws, Henning
2a91f4f9-11a1-4438-9248-e185d902e1d5
600
Takenberg, Meike
6d8dc7db-f4f1-422b-a824-76e47945e5d9
600
Nimtz, Manfred
709ca5f1-c9e0-485a-8109-70be748d8c0c
600
Getzlaff, Rita
4de8057a-ace2-479a-a5c3-fa538a16e695
600
Breithaupt, Dietmar E.
ada361b9-f73e-44e4-bdaa-75615e14b618
600
Berger, Ralf Günter
5629560f-8acb-4cd8-9f31-1d5fa59475c5
600
2016-03-04T11:42:37Z
2016-03-04T11:42:37Z
2005-05
Zorn, Holger; Bouws, H.; Takenberg, M.; Nimtz, M.; Getzlaff, R.; Breithaupt, D. E.; Berger, R. G.: An extracellular carboxylesterase from the basidiomycete Pleurotus sapidus hydrolyses xanthophyll esters. In: Biological Chemistry 386 (2005), Nr. 5, S. 435-440. DOI: http://dx.doi.org/10.1515/BC.2005.052
http://www.repo.uni-hannover.de/handle/123456789/259
http://dx.doi.org/10.15488/237
An extracellular enzyme capable of efficient hydrolysis of xanthophyll esters was purified from culture supernatants of the basidiomycete Pleurotus sapidus. Under native conditions, the enzyme exhibited a molecular mass of 430 kDa, and SDS-PAGE data suggested a composition of eight identical subunits. Biochemical characterisation of the purified protein showed an isoelectric point of 4.5, and ideal hydrolysis conditions were observed at pH 5.8 and 40 degrees C. Partial amino acid sequences were derived from N-terminal Edman degradation and from mass spectrometric ab initio sequencing of internal peptides. An 1861-bp cDNA containing an open reading frame of 1641 bp was cloned from a cDNA library that showed ca. 40% homology to Candida rugosa lipases. The P sapidus carboxylesterase represents the first enzyme of the lipase/esterase family from a basidiomycetous fungus that has been characterised at the molecular level.
Made available in DSpace on 2016-03-04T11:42:37Z (GMT). No. of bitstreams: 0
Previous issue date: 2005-05
publishedVersion
eng
Berlin : Walter de Gruyter
Biological Chemistry 386 (2005), Nr. 5
1437-4315
1431-6730
http://dx.doi.org/10.1515/BC.2005.052
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
alpha/beta-hydrolase fold
candida-rugosa lipases
capsicum-annuum l.
tagetes-erecta l.
cholesterol esterase
microbial lipases
swiss-model
proteins
biotechnology
carotenoids
cDNA library
fungi
lipase
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
580
600
An extracellular carboxylesterase from the basidiomycete Pleurotus sapidus hydrolyses xanthophyll esters
Article
Text
5
386
435
440
openAccess
Nationallizenz
ORIGINAL
bc.2005.052.pdf
bc.2005.052.pdf
application/pdf
178142
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123456789/259
oai:www.repo.uni-hannover.de:123456789/259
2022-12-13 16:12:26.669
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/2612022-12-13T15:12:26Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:580ddc:570
Zorn, Holger
bbf09d10-e7b6-4606-b1ec-257f9a1e82d2
600
Langhoff, Sabine
d2aea02e-e065-4204-bde6-3f2428d8c083
600
Scheibner, Manuela
1b687f85-95dd-4004-9851-51f07578a0cf
600
Nimtz, Manfred
709ca5f1-c9e0-485a-8109-70be748d8c0c
600
Berger, Ralf Günter
5629560f-8acb-4cd8-9f31-1d5fa59475c5
600
2016-03-04T11:42:38Z
2016-03-04T11:42:38Z
2003-07
Zorn, Holger; Langhoff, S.; Scheibner, M.; Nimtz, M.; Berger, R. G.: A peroxidase from Lepista irina cleaves beta,beta-carotene to flavor compounds. In: Biological Chemistry 384 (2003), Nr. 7, S. 1049-1056. DOI: http://dx.doi.org/10.1515/BC.2003.117
http://www.repo.uni-hannover.de/handle/123456789/261
http://dx.doi.org/10.15488/239
Extracellular liquid of the edible fungus Lepista irina was found to effectively degrade beta,beta-carotene. beta-Ionone, beta-cyclocitral, dihydroactinidiolide, and 2-hydroxy-2,6,6-trimethylcyclohexanone were formed as volatile breakdown products of beta,beta-carotene with myceliumfree culture supernatants, whereas beta-apo 10'-carotenal was identified as nonvolatile degradation product. The key enzyme catalyzing the oxidative cleavage of beta,beta-carotene was purified with an overall yield of 63% and a purification factor of 43. Biochemical characterization showed a molecular mass of 50.5 kDa and an isoelectric point of 3.75. Fastest beta,beta carotene degradation occurred at 34degreesC and pH values between 3.5 and 4. Degenerate oligonucleotides were derived from Nterminal and internal amino acid sequences. By means of PCRbased cDNAlibrary screening a 1284 bp cDNA was identified which showed great overall similarity to Pleurotus eryngii polyvalent peroxidases. The obtained sequence contains an open reading frame of 1083 nucleotides, encoding a polypeptide of 361 amino acids. A 30 amino acid signal peptide was identified upstream of the Nterminal sequence of the mature enzyme. The L. irina versatile peroxidase represents the first microbial enzyme capable of carotenoid degradation that has been characterized on a molecular level, proving the participation of extracellular enzymes of white rot fungi in biotic carotenoid degradation processes.
Made available in DSpace on 2016-03-04T11:42:38Z (GMT). No. of bitstreams: 0
Previous issue date: 2003-07
publishedVersion
eng
Berlin : Walter de Gruyter
Biological Chemistry 384 (2003), Nr. 7
1431-6730
http://dx.doi.org/10.1515/BC.2003.117
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
beta-carotene
pleurotus-eryngii
phanerochaete-chrysosporium
molecular characterization
aspergillus-niger
ionone
cooxidation
biotransformation
lipoxygenase
proteins
basidiomycete
cDNA
cleavage
degradation
norisoprenoids
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
580
600
A peroxidase from Lepista irina cleaves beta,beta-carotene to flavor compounds
Article
Text
7
384
1049
1056
openAccess
Nationallizenz
ORIGINAL
bc.2003.117.pdf
bc.2003.117.pdf
application/pdf
151717
https://www.repo.uni-hannover.de/bitstream/123456789/261/1/bc.2003.117.pdf
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bc.2003.117.pdf.txt
bc.2003.117.pdf.txt
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oai:www.repo.uni-hannover.de:123456789/261
2022-12-13 16:12:26.666
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
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Neuser, Frauke
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600
Zorn, Holger
bbf09d10-e7b6-4606-b1ec-257f9a1e82d2
600
Richter, Ulla
eaf3d3b7-37fe-4466-bfe9-5f06cfa79f73
600
Berger, Ralf Günter
5629560f-8acb-4cd8-9f31-1d5fa59475c5
600
2016-03-04T11:42:39Z
2016-03-04T11:42:39Z
2000-04
Neuser, F.; Zorn, Holger; Richter, U.; Berger, R. G.: Purification, characterisation and cDNA sequencing of pyruvate decarboxylase from Zygosaccharomyces bisporus. In: Biological Chemistry 381 (2000), Nr. 4, S. 349-353. DOI: http://dx.doi.org/10.1515/BC.2000.046
http://www.repo.uni-hannover.de/handle/123456789/263
http://dx.doi.org/10.15488/241
Cells of the wild-type yeast strain Zygosaccharomyces bisporus CBS 702 form alpha-hydroxy ketones from aromatic amino acid precursors during fermentation, Pyruvate decarboxylase (PDC, E.C. 4.1.1.1), the key enzyme of this biotransformation catalysing the nonoxidative decarboxylation of pyruvate and other 2-oxo-acids, was purified and characterised. The active enzyme is homotetrameric (alpha(4)) with a molecular mass of about 244 kDa, Activation of PDC by its substrate pyruvate results in a sigmoidal dependence of the reaction rate from substrate concentration (apparent K-m value 1.73 mM; Hill coefficient 2.10). A cDNA library was screened using a PCR-based procedure, and a 1856 bp cDNA of PDC was identified and sequenced. The cDNA encodes a polypeptide of 563 amino acid residues (monomeric unit), Sequence alignments demonstrate high homologies (> 80%) to PDC genes from Saccharomyces cerevisiae, Kluyveromyces lactis and Kluyveromyces marxianus.
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Previous issue date: 2000-04
DFG
publishedVersion
eng
Berlin : Walter de Gruyter
Biological Chemistry 381 (2000), Nr. 4
1431-6730
http://dx.doi.org/10.1515/BC.2000.046
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
brewers-yeast
pyrophosphate
binding
acyloin formation
enzyme purification
alpha-hydroxy ketones
non-conventional yeast
sequence alignment
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
580
600
Purification, characterisation and cDNA sequencing of pyruvate decarboxylase from Zygosaccharomyces bisporus
Article
Text
4
381
349
353
openAccess
Nationallizenz
ORIGINAL
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oai:www.repo.uni-hannover.de:123456789/263
2022-12-13 16:12:26.648
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/2752022-12-02T18:18:52Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Turgay, Kürşad
86f44485-2cb2-466b-85c1-54b209e12360
-1
Schäfer, Heinrich
4b319e7f-93fe-4d3b-bbbf-b663c5acf33d
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Hoßmann, Jörn
c832a43c-a350-4d27-b13e-2a03af820cc4
-1
Molière, Noël
c961a4c8-6f0c-47c3-ad91-7096c7130a82
-1
2016-03-22T13:53:02Z
2016-03-22T13:53:02Z
2016-03-16
Molière, N.; Hoßmann, J.; Schäfer H.; Turgay K.: Role of Hsp100/Clp Protease Complexes in Controlling the Regulation of Motility in Bacillus subtilis. In: Frontiers in Microbiology 7 (2016), Nr. 315. DOI: http://dx.doi.org/10.3389/fmicb.2016.00315
http://www.repo.uni-hannover.de/handle/123456789/275
http://dx.doi.org/10.15488/253
The Hsp100/Clp protease complexes of Bacillus subtilis ClpXP and ClpCP are involved in the control of many interconnected developmental and stress response regulatory networks, including competence, redox stress response, and motility. Here we analyzed the role of regulatory proteolysis by ClpXP and ClpCP in motility development. We have demonstrated that ClpXP acts on the regulation of motility by controlling the levels of the oxidative and heat stress regulator Spx. We obtained evidence that upon oxidative stress Spx not only induces the thiol stress response, but also transiently represses the transcription of flagellar genes. Furthermore, we observed that in addition to the known impact of ClpCP via the ComK/FlgM-dependent pathway, ClpCP also affects flagellar gene expression via modulating the activity and levels of the global regulator DegU-P. This adds another layer to the intricate involvement of Clp mediated regulatory proteolysis in different gene expression programs, which may allow to integrate and coordinate different signals for a better-adjusted response to the changing environment of B. subtilis cells.
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Previous issue date: 2016-03-16
DFG/Tu106/6
DFG/Tu106/7
publishedVersion
eng
Lausanne : Frontiers
Frontiers in Microbiology 7 (2016)
1664-302X
http://dx.doi.org/10.3389/fmicb.2016.00315
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
AAA+ proteins
regulatory proteolysis
ClpC
ClpX
ClpP
motility
Bacillus subtilis
Mikrobiologie
Heubazillus
Proteolyse
Clp
Mikrobiologie
Heubazillus
Proteolyse
Clp <Protease>
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Role of Hsp100/Clp Protease Complexes in Controlling the Regulation of Motility in Bacillus subtilis
Article
Text
7
openAccess
Publikation gefördert durch den Publikationsfonds der Leibniz Universität Hannover.
Frontiers in Microbiology 7 (2016)
Kürşad
Heinrich
Jörn
Noël
Turgay
Schäfer
Hoßmann
Molière
LUH_Fonds
ORIGINAL
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2022-12-02 19:18:52.134
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
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oai:www.repo.uni-hannover.de:123456789/2772022-12-02T16:14:10Zcom_123456789_1col_123456789_8ddc:550doc-type:Articledoc-type:Textddc:630open_accessstatus-type:publishedVersionddc:570
Bachmann, Jörg
d321d71f-a22e-4fee-936f-3be8f67fc52f
600
Goebel, Marc-Oliver
cf66a934-1a98-48e3-9f3f-16410a534967
600
Woche, Susanne K.
4eda236b-15c7-450f-b4e0-4edaa898cd17
600
2016-03-23T08:29:25Z
2016-03-23T08:29:25Z
2013
Bachmann, Joerg; Goebel, Marc-O.; Woche, Susanne K.: Small-scale contact angle mapping on undisturbed soil surfaces. In: Journal of Hydrology and Hydromechanics 61 (2013), Nr. 1, S. 3-8. DOI: http://dx.doi.org/10.2478/johh-2013-0002
http://www.repo.uni-hannover.de/handle/123456789/277
http://dx.doi.org/10.15488/255
Research of the last years pointed out that most soils are neither completely hydrophilic nor hydrophobic, but exhibit a subcritical level of water repellency (i.e. contact angle, CA > 0 degrees and < 90 degrees). Soil water repellency (SWR) is mainly caused by organic compounds of different origin and structure, showing the relevance of biofilms and organic coatings present at many particle surfaces. Despite the importance of SWR for hydraulic processes like preferential flow phenomena, generation of heterogeneous moisture patterns, or surface run-off generation, detailed investigations on the spatial variability of SWR at various scales have rarely been carried out. We introduce a new and easy-to-apply operation for measuring the spatial distribution of SWR using a modified sessile drop method for direct optical assessment of CA at a small scale. The specific objectives of this paper are to apply a sampling and preparation technique that preserves the original spatial arrangement of soil particles and to characterize soil wettability in terms of CA at a high spatial resolution. Results revealed that the sampling and preparation technique allows determination of CA at the millimeter scale using droplets of 1 mu L volume. Direct measurement on grain surfaces of the sand fraction is possible for grain sizes > 300 mu m using drop volumes down to 0.1 mu L. Geostatistical evaluation showed that the measurement grid scale is below the range of spatial dependency for droplets of 1 mu L volume, but not for measurements on single grains (pure nugget effect). Results show further that the small-scale differences in wettability, especially for CA < 90 degrees, cannot be detected by the conventional WDPT test. From these findings it can be concluded that the proposed technique allows the identification of small-scale variations in wettability that may promote the formation of heterogeneous flow fields and moisture patterns in soil under unsaturated conditions.
Made available in DSpace on 2016-03-23T08:29:25Z (GMT). No. of bitstreams: 0
Previous issue date: 2013
DFG/SPP/1315
DFG/BA1359/9-2
publishedVersion
eng
Berlin : Walter de Gruyter
Journal of Hydrology and Hydromechanics 61 (2013), Nr. 1
0042-790X
http://dx.doi.org/10.2478/johh-2013-0002
CC BY-NC-ND 3.0 Unported
http://creativecommons.org/licenses/by-nc-nd/3.0/
Hydrophobicity
Sessile drop method
Soil water repellency
Water drop penetration time
Wettability
water-repellency
organic-matter
porous-media
wettability
decomposition
sorptivity
ethanol
flow
Dewey Decimal Classification::500 | Naturwissenschaften::550 | Geowissenschaften
550
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::600 | Technik::630 | Landwirtschaft, Veterinärmedizin
630
600
Small-scale contact angle mapping on undisturbed soil surfaces
Article
Text
1
61
3
8
openAccess
Von: rights@degruyter.com An: Koch, Melanie Betreff: AW: WG: Lizenzbestimmungen unklar [ ref:_00DD0qhxc._5005718Js1R:ref ] Datum: Dienstag, 22. März 2016 16:58:45 Liebe Frau Klatte, ja, es handelt sich um CC BY-NC-ND 3.0. Beste Grüße, De Gruyter Rights & Licenses Team Tanja Linhardt Associate Rights & Licenses DE GRUYTER Genthiner Straße 13 10785 Berlin, Germany rights@degruyter.com www.degruyter.com Walter de Gruyter GmbH. Genthiner Str. 13. 10785 Berlin. Domicile Berlin. Amtsgericht Charlottenburg HRB 143490 B. Rechtsform: GmbH. Executive Board: Dr. Anke Beck, Carsten Buhr Chairman of the Supervisory Board: Rüdiger Gebauer Find us on Facebook: www.facebook.com/degruyter.publishers Newsletter and Alerts: www.degruyter.com/newsletter Von: Koch, Melanie [mailto:Melanie.Koch@tib.eu] Gesendet: Mittwoch, 16. März 2016 12:05 An: Linhardt, Tanja <Tanja.Linhardt@degruyter.com> Cc: Klatte, Silke <Silke.Klatte@tib.eu> Betreff: AW: WG: Lizenzbestimmungen unklar [ ref:_00DD0qhxc._5005718Js1R:ref ] Liebe Frau Lindhardt, eine kurze Frage habe ich nun doch: Handelt es sich um CC BY-NC-ND 3.0 - https://creativecommons.org/licenses/by-nc-nd/3.0/? Viele Grüße und vielen Dank im Voraus für Ihre Mühe. Melanie Koch Technische Informationsbibliothek (TIB) Team Publikationsdienste Abteilung Benutzungs- und Informationsdienste Welfengarten 1 B // 30167 Hannover T 0511 762-19869 // F 0511 762-29 24 melanie.koch@tib.eu www.tib.eu www.repo.uni-hannover.de Von: Tanja.Linhardt@degruyter.com [mailto:Tanja.Linhardt@degruyter.com] Gesendet: Montag, 14. März 2016 17:04 An: Koch, Melanie Betreff: WG: WG: Lizenzbestimmungen unklar [ ref:_00DD0qhxc._5005718Js1R:ref ] Sehr geehrte Frau Koch, bei den aufgeführten Titeln handelt es sich um CC-BY-NC-ND Lizenzen. Beste Grüße, De Gruyter Rights & Licenses Team Tanja Linhardt Associate Rights & Licenses DE GRUYTER Genthiner Straße 13 10785 Berlin, Germany rights@degruyter.com www.degruyter.com Walter de Gruyter GmbH. Genthiner Str. 13. 10785 Berlin. Domicile Berlin. Amtsgericht Charlottenburg HRB 143490 B. Rechtsform: GmbH. Executive Board: Dr. Anke Beck, Carsten Buhr Chairman of the Supervisory Board: Rüdiger Gebauer Find us on Facebook: www.facebook.com/degruyter.publishers Newsletter and Alerts: www.degruyter.com/newsletter WG: Lizenzbestimmungen unklar Von: Koch, Melanie [mailto:Melanie.Koch@tib.eu] Gesendet: Dienstag, 16. Februar 2016 16:09 An: service@degruyter.com Betreff: Lizenzbestimmungen unklar Sehr geehrte Damen und Herren, folgende Artikel sind als Open Access gekennzeichnet. Könnten Sie mir bitte mitteilen, ob bzw. welche Creative-Commons-Lizenzen genau hier gelten? Ich habe nämlich in den Artikeln keinen entsprechenden Hinweis gefunden: · http://dx.doi.org/10.2478/johh-2013-0002 · http://dx.doi.org/10.2478/johh-2013-0007 · http://dx.doi.org/10.2478/remav-2013-0020 · http://dx.doi.org/10.2478/v10303-012-0004-5 · http://dx.doi.org/10.2478/v10177-010-0047-7 · http://dx.doi.org/10.1524/ncrs.2003.218.3.275 · http://dx.doi.org/10.1524/ncrs.2003.218.4.373 Über eine Rückmeldung würde ich mich sehr freuen. Viele Grüße Melanie Koch Technische Informationsbibliothek (TIB) Standort Conti-Campus Team Publikationsdienste Abteilung Benutzungs- und Informationsdienste Königsworther Platz 1 B // 30167 Hannover T 0511 762-19869 // F 0511 762-29 24 melanie.koch@tib.eu<mailto:melanie.koch@tib.eu> www.tib.eu<http://www.tib.eu> www.repo.uni-hannover.de<http://www.repo.uni-hannover.de> ref:_00DD0qhxc._5005718Js1R:ref
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Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/2782022-12-02T16:10:15Zcom_123456789_1col_123456789_8ddc:550doc-type:Articledoc-type:Textddc:630open_accessstatus-type:publishedVersionddc:570
Schaumann, Gabriele E.
61973147-07a6-4ff4-8899-3d69b8e6d0b1
600
Diehl, Dörte
5d466e10-cafb-4be6-84bb-e1e328d951d3
600
Bertmer, Marko
4254b66e-a3b2-4ef6-9d88-a2e861889a40
600
Jaeger, Alexander
45649c2d-a808-4a92-8de0-993acd97703f
600
Conte, Pellegrino
486fe6fe-0cd9-4780-af84-2a3a40bc703e
600
Alonzo, Giuseppe
a324b803-cbff-4d5e-a035-48e10882ebd0
600
Bachmann, Jörg
d321d71f-a22e-4fee-936f-3be8f67fc52f
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2016-03-23T08:29:26Z
2016-03-23T08:29:26Z
2013
Schaumann, Gabriele E.; Diehl, Doerte; Bertmer, Marko; Jaeger, Alexander; Conte, Pellegrino; Alonzo, Giuseppe; Bachmann, Joerg: Combined proton NMR wideline and NMR relaxometry to study SOM-water interactions of cation-treated soils. In: Journal of Hydrology and Hydromechanics 61 (2013), Nr. 1, S. 50-63. DOI: http://dx.doi.org/10.2478/johh-2013-0007
http://www.repo.uni-hannover.de/handle/123456789/278
http://dx.doi.org/10.15488/256
Focusing on the idea that multivalent cations affect SOM matrix and surface, we treated peat and soil samples by solutions of NaCl, CaCl2 or AlCl3. Water binding was characterized with low field H-1-NMR-relaxometry (20 MHz) and H-1 wideline NMR spectroscopy (400 MHz) and compared to contact angles. From H-1 wideline, we distinguished mobile water and water involved in water molecule bridges (WaMB). Large part of cation bridges (CaB) between SOM functional groups are associated with WaMB. Unexpectedly, H-1 NMR-relaxometry relaxation rates suggest that cross-linking in the Al-containing peat is not stronger than that by Ca. The relation between percentage of mobile water and WaMB water in the context of wettability and H-1 NMR relaxation times confirms that wettability controls the water film surrounding soil particles. Wettability is controlled by WaMB-CaB associations fixing hydrophilic functional groups in the SOM interior. This can lead to severe water repellency. Wettability decreases with increasing involvement of functional groups in CaB-WaMB associations. The results demonstrate the relevance of CaB and WaMB for the dynamics of biogeochemical and hydrological processes under field conditions, as only a few percent of organic matter can affect the physical, chemical, and biological functioning of the entire 3-phase ecosystem.
Made available in DSpace on 2016-03-23T08:29:26Z (GMT). No. of bitstreams: 0
Previous issue date: 2013
DFG/SPP1315/SCHA849/8
DFG/SPP1315/BA1359/9
publishedVersion
eng
Berlin : Walter de Gruyter
Journal of Hydrology and Hydromechanics 61 (2013), Nr. 1
0042-790X
http://dx.doi.org/10.2478/johh-2013-0007
CC BY-NC-ND 3.0 Unported
http://creativecommons.org/licenses/by-nc-nd/3.0/
Soil organic matter (SOM)
Low field H-1 NMR relaxometry
H-1 wideline NMR spectroscopy
Contact angle
Cation bridges
Water molecule bridges
natural organic-matter
multiexponential decay data
nuclear-magnetic-resonance
uniform-penalty inversion
humic substances
contact-angle
forest soils
sandy soil
repellency
state
Dewey Decimal Classification::500 | Naturwissenschaften::550 | Geowissenschaften
550
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::600 | Technik::630 | Landwirtschaft, Veterinärmedizin
630
600
Combined proton NMR wideline and NMR relaxometry to study SOM-water interactions of cation-treated soils
Article
Text
1
61
50
63
openAccess
Von: rights@degruyter.com An: Koch, Melanie Betreff: AW: WG: Lizenzbestimmungen unklar [ ref:_00DD0qhxc._5005718Js1R:ref ] Datum: Dienstag, 22. März 2016 16:58:45 Liebe Frau Klatte, ja, es handelt sich um CC BY-NC-ND 3.0. Beste Grüße, De Gruyter Rights & Licenses Team Tanja Linhardt Associate Rights & Licenses DE GRUYTER Genthiner Straße 13 10785 Berlin, Germany rights@degruyter.com www.degruyter.com Walter de Gruyter GmbH. Genthiner Str. 13. 10785 Berlin. Domicile Berlin. Amtsgericht Charlottenburg HRB 143490 B. Rechtsform: GmbH. Executive Board: Dr. Anke Beck, Carsten Buhr Chairman of the Supervisory Board: Rüdiger Gebauer Find us on Facebook: www.facebook.com/degruyter.publishers Newsletter and Alerts: www.degruyter.com/newsletter Von: Koch, Melanie [mailto:Melanie.Koch@tib.eu] Gesendet: Mittwoch, 16. März 2016 12:05 An: Linhardt, Tanja <Tanja.Linhardt@degruyter.com> Cc: Klatte, Silke <Silke.Klatte@tib.eu> Betreff: AW: WG: Lizenzbestimmungen unklar [ ref:_00DD0qhxc._5005718Js1R:ref ] Liebe Frau Lindhardt, eine kurze Frage habe ich nun doch: Handelt es sich um CC BY-NC-ND 3.0 - https://creativecommons.org/licenses/by-nc-nd/3.0/? Viele Grüße und vielen Dank im Voraus für Ihre Mühe. Melanie Koch Technische Informationsbibliothek (TIB) Team Publikationsdienste Abteilung Benutzungs- und Informationsdienste Welfengarten 1 B // 30167 Hannover T 0511 762-19869 // F 0511 762-29 24 melanie.koch@tib.eu www.tib.eu www.repo.uni-hannover.de Von: Tanja.Linhardt@degruyter.com [mailto:Tanja.Linhardt@degruyter.com] Gesendet: Montag, 14. März 2016 17:04 An: Koch, Melanie Betreff: WG: WG: Lizenzbestimmungen unklar [ ref:_00DD0qhxc._5005718Js1R:ref ] Sehr geehrte Frau Koch, bei den aufgeführten Titeln handelt es sich um CC-BY-NC-ND Lizenzen. Beste Grüße, De Gruyter Rights & Licenses Team Tanja Linhardt Associate Rights & Licenses DE GRUYTER Genthiner Straße 13 10785 Berlin, Germany rights@degruyter.com www.degruyter.com Walter de Gruyter GmbH. Genthiner Str. 13. 10785 Berlin. Domicile Berlin. Amtsgericht Charlottenburg HRB 143490 B. Rechtsform: GmbH. Executive Board: Dr. Anke Beck, Carsten Buhr Chairman of the Supervisory Board: Rüdiger Gebauer Find us on Facebook: www.facebook.com/degruyter.publishers Newsletter and Alerts: www.degruyter.com/newsletter WG: Lizenzbestimmungen unklar Von: Koch, Melanie [mailto:Melanie.Koch@tib.eu] Gesendet: Dienstag, 16. Februar 2016 16:09 An: service@degruyter.com Betreff: Lizenzbestimmungen unklar Sehr geehrte Damen und Herren, folgende Artikel sind als Open Access gekennzeichnet. Könnten Sie mir bitte mitteilen, ob bzw. welche Creative-Commons-Lizenzen genau hier gelten? Ich habe nämlich in den Artikeln keinen entsprechenden Hinweis gefunden: · http://dx.doi.org/10.2478/johh-2013-0002 · http://dx.doi.org/10.2478/johh-2013-0007 · http://dx.doi.org/10.2478/remav-2013-0020 · http://dx.doi.org/10.2478/v10303-012-0004-5 · http://dx.doi.org/10.2478/v10177-010-0047-7 · http://dx.doi.org/10.1524/ncrs.2003.218.3.275 · http://dx.doi.org/10.1524/ncrs.2003.218.4.373 Über eine Rückmeldung würde ich mich sehr freuen. Viele Grüße Melanie Koch Technische Informationsbibliothek (TIB) Standort Conti-Campus Team Publikationsdienste Abteilung Benutzungs- und Informationsdienste Königsworther Platz 1 B // 30167 Hannover T 0511 762-19869 // F 0511 762-29 24 melanie.koch@tib.eu<mailto:melanie.koch@tib.eu> www.tib.eu<http://www.tib.eu> www.repo.uni-hannover.de<http://www.repo.uni-hannover.de> ref:_00DD0qhxc._5005718Js1R:ref
ORIGINAL
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MD5
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oai:www.repo.uni-hannover.de:123456789/278
2022-12-02 17:10:15.371
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
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Quibod, Ian Lorenzo
2a4fb141-5d34-4dc9-8326-cfb73ccb2c35
Grande, Genelou
4dd7bdfb-39fe-4b23-a238-3214bd0098d1
Oreiro, Eula Gems
5ec83459-6931-4991-bc54-ae4aab7f5b28
Borja, Frances Nikki
d2d21fe9-7d44-408e-95ee-7e89bf1a7da0
Dossa, Gerbert Sylvestre
48539ea7-a4e9-4281-9bab-f3dff039c98f
Mauleon, Ramil
0ad97007-ef2b-462e-b56d-24d8deb78e48
Cruz, Casiana Vera
6daae30b-058d-44d2-bb7b-a8b83c5bd6d8
Oliva, Ricardo
f2b4961d-bcca-4970-899d-04bc0e997de1
2016-06-13T14:30:38Z
2016-06-13T14:30:38Z
2015-09-30
Quibod, Ian Lorenzo; Grande, Genelou; Oreiro, Eula Gems; Borja, Frances Nikki; Dossa, Gerbert Sylvestre; Mauleon, Ramil; Cruz, Casiana Vera; Oliva, Ricardo: Rice-Infecting Pseudomonas Genomes Are Highly Accessorized and Harbor Multiple Putative Virulence Mechanisms to Cause Sheath Brown Rot. In: PloS ONE 10 (2015), Nr. 9, e0139256. DOI: http://dx.doi.org/10.1371/journal.pone.0139256
http://www.repo.uni-hannover.de/handle/123456789/290
http://dx.doi.org/10.15488/268
Sheath rot complex and seed discoloration in rice involve a number of pathogenic bacteria that cannot be associated with distinctive symptoms. These pathogens can easily travel on asymptomatic seeds and therefore represent a threat to rice cropping systems. Among the rice-infecting Pseudomonas, P. fuscovaginae has been associated with sheath brown rot disease in several rice growing areas around the world. The appearance of a similar Pseudomonas population, which here we named P. fuscovaginae-like, represents a perfect opportunity to understand common genomic features that can explain the infection mechanism in rice. We showed that the novel population is indeed closely related to P. fuscovaginae. A comparative genomics approach on eight rice-infecting Pseudomonas revealed heterogeneous genomes and a high number of strain-specific genes. The genomes of P. fuscovaginae-like harbor four secretion systems (Type I, II, III, and VI) and other important pathogenicity machinery that could probably facilitate rice colonization. We identified 123 core secreted proteins, most of which have strong signatures of positive selection suggesting functional adaptation. Transcript accumulation of putative pathogenicity-related genes during rice colonization revealed a concerted virulence mechanism. The study suggests that rice-infecting Pseudomonas causing sheath brown rot are intrinsically diverse and maintain a variable set of metabolic capabilities as a potential strategy to occupy a range of environments.
Made available in DSpace on 2016-06-13T14:30:38Z (GMT). No. of bitstreams: 0
Previous issue date: 2015-09-30
Consortium for International Agricultural Research (CGIAR)
Global Rice Science Partnership (GRiSP)
publishedVersion
eng
San Francisco : Public Library Science
PLoS ONE 10 (2015), Nr. 9
1932-6203
http://dx.doi.org/10.1371/journal.pone.0139256
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
III secretion system
microbial pan-genome
phytopathogenic bacteria
grain discoloration
species definition
fluorescens f113
fuscovaginae
pathogen
sequence
host
Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
580
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Rice-Infecting Pseudomonas Genomes Are Highly Accessorized and Harbor Multiple Putative Virulence Mechanisms to Cause Sheath Brown Rot
Article
Text
9
10
e0139256
openAccess
ORIGINAL
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journal.pone.0139256.pdf
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2022-12-02 17:14:10.662
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/2912022-12-02T16:14:11Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:580ddc:570
Fleck, Alexander T.
67616ebc-6089-4299-915c-8ff53f976420
600
Schulze, Sascha
90e8bffb-03e8-484f-b37a-2b0514f9cc6b
600
Hinrichs, Martin
032976fc-5f93-4d3f-9337-8fa4c1b4f5ec
600
Specht, Andre
a583a0ae-61c2-4947-aadb-755d96c97446
600
Wassmann, Friedrich
ea13e6d1-065a-4dc0-b69c-d72b7a49b643
600
Schreiber, Lukas
c1413e3b-a738-4406-8d46-a144a00ceda9
600
Schenk, Manfred K.
d35d9129-97c5-4894-bd95-614d853e8c01
600
2016-06-13T14:30:39Z
2016-06-13T14:30:39Z
2015-09-18
Fleck, Alexander T.; Schulze, Sascha; Hinrichs, Martin; Specht, Andre; Wassmann, Friedrich; Schreiber, Lukas; Schenk, Manfred K.: Silicon Promotes Exodermal Casparian Band Formation in Si-Accumulating and Si-Excluding Species by Forming Phenol Complexes. In: PloS ONE 10 (2015), Nr. 9, e0138555. DOI: http://dx.doi.org/10.1371/journal.pone.0138555
http://www.repo.uni-hannover.de/handle/123456789/291
http://dx.doi.org/10.15488/269
We studied the effect of Silicon (Si) on Casparian band (CB) development, chemical composition of the exodermal CB and Si deposition across the root in the Si accumulators rice and maize and the Si non-accumulator onion. Plants were cultivated in nutrient solution with and without Si supply. The CB development was determined in stained root cross-sections. The outer part of the roots containing the exodermis was isolated after enzymatic treatment. The exodermal suberin was transesterified with MeOH/BF3 and the chemical composition was measured using gas chromatography-mass spectroscopy (GC-MS) and flame ionization detector (GC-FID). Laser ablation-inductively coupled plasma-mass spectroscopy (LA-ICP-MS) was used to determine the Si deposition across root cross sections. Si promoted CB formation in the roots of Si-accumulator and Si non-accumulator species. The exodermal suberin was decreased in rice and maize due to decreased amounts of aromatic suberin fractions. Si did not affect the concentration of lignin and lignin-like polymers in the outer part of rice, maize and onion roots. The highest Si depositions were found in the tissues containing CB. These data along with literature were used to suggest a mechanism how Si promotes the CB development by forming complexes with phenols.
Made available in DSpace on 2016-06-13T14:30:39Z (GMT). No. of bitstreams: 0
Previous issue date: 2015-09-18
DFG/SCHR 506/12-1
publishedVersion
eng
San Francisco : Public Library Science
PLoS ONE 10 (2015), Nr. 9
1932-6203
http://dx.doi.org/10.1371/journal.pone.0138555
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
rice oryza-sativa
radial hydraulic conductivity
zea-mays l.
endodermal cell-walls
reduces sodium uptake
chemical-composition
apoplastic barriers
lignin biosynthesis
saline conditions
clivia-miniata
Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
580
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Silicon Promotes Exodermal Casparian Band Formation in Si-Accumulating and Si-Excluding Species by Forming Phenol Complexes
Article
Text
9
10
e0138555
openAccess
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2022-12-02 17:14:11.03
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/2932022-12-02T16:11:40Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessddc:590status-type:publishedVersionddc:580ddc:570
Schlinkert, Hella
6bf326bc-1823-4a7c-b39e-480de3389d06
Westphal, Catrin
34da4141-9891-4284-81db-8fb589e58eac
Clough, Yann
fcb687c7-ca69-4cff-a190-4d7905c581ec
Laszlo, Zoltan
10e39008-2bea-4341-b2a5-20e5a873c3e7
Ludwig, Martin
15cb481b-f481-408f-8291-0cc737ac2151
Tscharntke, Teja
cebf3476-d236-4c3a-ae4b-3090c078f1d0
2016-06-13T14:30:39Z
2016-06-13T14:30:39Z
2015-08-20
Schlinkert, Hella; Westphal, Catrin; Clough, Yann; Laszlo, Zoltan; Ludwig, Martin; Tscharntke, Teja: Plant Size as Determinant of Species Richness of Herbivores, Natural Enemies and Pollinators across 21 Brassicaceae Species. In: PloS ONE 10 (2015), Nr. 8, e0135928. DOI: http://dx.doi.org/10.1371/journal.pone.0135928
http://www.repo.uni-hannover.de/handle/123456789/293
http://dx.doi.org/10.15488/271
Large plants are often more conspicuous and more attractive for associated animals than small plants, e.g. due to their wider range of resources. Therefore, plant size can positively affect species richness of associated animals, as shown for single groups of herbivores, but studies usually consider intraspecific size differences of plants in unstandardised environments. As comprehensive tests of interspecific plant size differences under standardised conditions are missing so far, we investigated effects of plant size on species richness of all associated arthropods using a common garden experiment with 21 Brassicaceae species covering a broad interspecific plant size gradient from 10 to 130 cm height. We recorded plant associated ecto-and endophagous herbivores, their natural enemies and pollinators on and in each aboveground plant organ, i.e. flowers, fruits, leaves and stems. Plant size (measured as height from the ground), the number of different plant organ entities and their biomass were assessed. Increasing plant size led to increased species richness of associated herbivores, natural enemies and pollinating insects. This pattern was found for ectophagous and endophagous herbivores, their natural enemies, as well as for herbivores associated with leaves and fruits and their natural enemies, independently of the additional positive effects of resource availability (i.e. organ biomass or number of entities and, regarding natural enemies, herbivore species richness). We found a lower R-2 for pollinators compared to herbivores and natural enemies, probably caused by the high importance of flower characteristics for pollinator species richness besides plant size. Overall, the increase in plant height from 10 to 130 cm led to a 2.7-fold increase in predicted total arthropod species richness. In conclusion, plant size is a comprehensive driver of species richness of the plant associated arthropods, including pollinators, herbivores and their natural enemies, whether they are endophagous or ectophagous or associated with leaves or fruits.
Made available in DSpace on 2016-06-13T14:30:39Z (GMT). No. of bitstreams: 0
Previous issue date: 2015-08-20
German State of Lower Saxony/Cluster of Excellence "Functional Biodiversity Research"
State of Lower Saxony/Cluster of Excellence "Functional Biodiversity Research"
publishedVersion
eng
San Francisco : Public Library Science
PLoS ONE 10 (2015), Nr. 8
1932-6203
http://dx.doi.org/10.1371/journal.pone.0135928
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
body-size
phytophagous insects
architecture
diversity
parasitoids
communities
specificity
visitation
predators
evolution
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
580
600
Dewey Decimal Classification::500 | Naturwissenschaften::590 | Tiere (Zoologie)
590
600
Plant Size as Determinant of Species Richness of Herbivores, Natural Enemies and Pollinators across 21 Brassicaceae Species
Article
Text
8
10
e0135928
openAccess
ORIGINAL
journal.pone.0135928.pdf
journal.pone.0135928.pdf
application/pdf
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2022-12-02 17:11:40.786
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/2982022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessddc:590status-type:publishedVersionddc:570
Njihia, Teresiah Nyambura
37cbb1fd-8458-44b3-878e-0721e6dabc32
600
Jaramillo, Juliana
7e2c995f-65fa-4f3e-9c18-368bb37d67cf
600
Murungi, Lucy
93318a09-a77e-41b3-bedd-8809a9b10ce6
600
Mwenda, Dickson
2de1d9a6-f4a3-4ca3-98fb-3a264a1343f4
600
Orindi, Benedict
907cd818-d3ac-47b0-a8d6-b7db29d51496
600
Poehling, Hans-Michael
84b8da68-9ed7-4a7f-a98c-29ba64f9092c
600
Torto, Baldwyn
3677a953-5475-492e-9b93-2ceea498f45f
600
2016-06-13T14:30:49Z
2016-06-13T14:30:49Z
2014-11-07
Njihia, Teresiah Nyambura; Jaramillo, Juliana; Murungi, Lucy; Mwenda, Dickson; Orindi, Benedict; Poehling, Hans-Michael; Torto, Baldwyn: Spiroacetals in the Colonization Behaviour of the Coffee Berry Borer: A 'Push-Pull' System. In: PloS ONE 9 (2014), Nr. 11, e111316. DOI: http://dx.doi.org/10.1371/journal.pone.0111316
http://www.repo.uni-hannover.de/handle/123456789/298
http://dx.doi.org/10.15488/276
Coffee berries are known to release several volatile organic compounds, among which is the spiroacetal, conophthorin, an attractant for the coffee berry borer Hypothenemus hampei. Elucidating the effects of other spiroacetals released by coffee berries is critical to understanding their chemo-ecological roles in the host discrimination and colonization process of the coffee berry borer, and also for their potential use in the management of this pest. Here, we show that the coffee berry spiroacetals frontalin and 1,6-dioxaspiro [4.5] decane (referred thereafter as brocain), are also used as semiochemicals by the coffee berry borer for host colonization. Bioassays and chemical analyses showed that crowding coffee berry borers from 2 to 6 females per berry, reduced borer fecundity, which appeared to correlate with a decrease in the emission rates of conophthorin and frontalin over time. In contrast, the level of brocain did not vary significantly between borer-uninfested and infested berries. Brocain was attractive at lower doses, but repellent at higher doses while frontalin alone or in a blend was critical for avoidance. Field assays with a commercial attractant comprising a mixture of ethanol and methanol (1:1), combined with frontalin, confirmed the repellent effect of this compound by disrupting capture rates of H. hampei females by 77% in a coffee plantation. Overall, our results suggest that the levels of frontalin and conophthorin released by coffee berries determine the host colonization behaviour of H. hampei, possibly through a 'push-pull' system, whereby frontalin acts as the 'push' (repellent) and conophthorin acting as the 'pull' (attractant). Furthermore, our results reveal the potential use of frontalin as a repellent for management of this coffee pest.
Made available in DSpace on 2016-06-13T14:30:49Z (GMT). No. of bitstreams: 0
Previous issue date: 2014-11-07
DFG
publishedVersion
eng
San Francisco : Public Library Science
PLoS ONE 9 (2014), Nr. 11
1932-6203
http://dx.doi.org/10.1371/journal.pone.0111316
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
mountain pine-beetle
hypothenemus-hampei coleoptera
dendroctonus-ponderosae hopkins
bark beetle
ips-typographus
curculionidae scolytinae
pheromone production
field response
volatiles
semiochemicals
Dewey Decimal Classification::500 | Naturwissenschaften::590 | Tiere (Zoologie)
590
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Spiroacetals in the Colonization Behaviour of the Coffee Berry Borer: A 'Push-Pull' System
Article
Text
11
9
e111316
openAccess
ORIGINAL
journal.pone.0111316.pdf
journal.pone.0111316.pdf
application/pdf
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https://www.repo.uni-hannover.de/bitstream/123456789/298/1/journal.pone.0111316.pdf
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oai:www.repo.uni-hannover.de:123456789/298
2022-12-02 17:14:10.351
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/2992022-12-02T16:11:40Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:580ddc:570
Muvea, Alexander M.
3be50efe-36e6-454d-8c45-2eb438bbcab8
600
Meyhöfer, Rainer
e779ffa5-b734-466d-bd85-c13de94b68ec
600
Subramanian, Sevgan
f28faf84-1528-4c04-880e-02da22e40a44
600
Poehling, Hans-Michael
84b8da68-9ed7-4a7f-a98c-29ba64f9092c
600
Ekesi, Sunday
bbd7feed-e359-42b1-8c60-1acd1c630021
600
Maniania, Nguya K.
ae520cfc-3965-4bb7-8340-dd3a17ebd231
600
2016-06-13T14:30:49Z
2016-06-13T14:30:49Z
2014-09-25
Muvea, Alexander M.; Meyhoefer, Rainer; Subramanian, Sevgan; Poehling, Hans-Michael; Ekesi, Sunday; Maniania, Nguya K.: Colonization of Onions by Endophytic Fungi and Their Impacts on the Biology of Thrips tabaci. In: PloS ONE 9 (2014), Nr. 9, e108242. DOI: http://dx.doi.org/10.1371/journal.pone.0108242
http://www.repo.uni-hannover.de/handle/123456789/299
http://dx.doi.org/10.15488/277
Endophytic fungi, which live within host plant tissues without causing any visible symptom of infection, are important mutualists that mediate plant-herbivore interactions. Thrips tabaci (Lindeman) is one of the key pests of onion, Allium cepa L., an economically important agricultural crop cultivated worldwide. However, information on endophyte colonization of onions, and their impacts on the biology of thrips feeding on them, is lacking. We tested the colonization of onion plants by selected fungal endophyte isolates using two inoculation methods. The effects of inoculated endophytes on T. tabaci infesting onion were also examined. Seven fungal endophytes used in our study were able to colonize onion plants either by the seed or seedling inoculation methods. Seed inoculation resulted in 1.47 times higher mean percentage post-inoculation recovery of all the endophytes tested as compared to seedling inoculation. Fewer thrips were observed on plants inoculated with Clonostachys rosea ICIPE 707, Trichoderma asperellum M2RT4, Trichoderma atroviride ICIPE 710, Trichoderma harzianum 709, Hypocrea lixii F3ST1 and Fusarium sp. ICIPE 712 isolates as compared to those inoculated with Fusarium sp. ICIPE 717 and the control treatments. Onion plants colonized by C. rosea ICIPE 707, T. asperellum M2RT4, T. atroviride ICIPE 710 and H. lixii F3ST1 had significantly lower feeding punctures as compared to the other treatments. Among the isolates tested, the lowest numbers of eggs were laid by T. tabaci on H. lixii F3ST1 and C. rosea ICIPE 707 inoculated plants. These results extend the knowledge on colonization of onions by fungal endophytes and their effects on Thrips tabaci.
Made available in DSpace on 2016-06-13T14:30:49Z (GMT). No. of bitstreams: 0
Previous issue date: 2014-09-25
BMZ/GIZ/11.7860.7-001.00
BMZ/GIZ/11.7860.7-001.00
publishedVersion
eng
San Francisco : Public Library Science
PLoS ONE 9 (2014), Nr. 9
1932-6203
http://dx.doi.org/10.1371/journal.pone.0108242
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
yellow-spot-virus
beauveria-bassiana ascomycota
banana musa-spp.
frankliniella-occidentalis
metarhizium-anisopliae
thysanoptera thripidae
cosmopolites-sordidus
balsamo vuillemin
damage
inoculation
Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
580
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Colonization of Onions by Endophytic Fungi and Their Impacts on the Biology of Thrips tabaci
Article
Text
9
9
e108242
openAccess
ORIGINAL
journal.pone.0108242.pdf
journal.pone.0108242.pdf
application/pdf
456709
https://www.repo.uni-hannover.de/bitstream/123456789/299/1/journal.pone.0108242.pdf
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123456789/299
oai:www.repo.uni-hannover.de:123456789/299
2022-12-02 17:11:40.797
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/3142022-12-02T15:17:13Zcom_123456789_1col_123456789_7doc-type:Articledoc-type:Textopen_accessddc:530status-type:publishedVersionddc:610ddc:510ddc:570
Eickhoff, Rene
ddca4e25-6c44-4de2-9f11-0d15eccf8671
600
Lorbeer, Raoul-Amadeus
d8bffbb9-f29a-4851-bed3-6c5f7c4c3fe6
600
Scheiblich, Hannah
62a6cceb-45ab-4a87-8170-f81a4ef72c60
600
Heisterkamp, Alexander
67fb34de-b181-4db7-b626-59482982c9b3
600
Meyer, Heiko
6e649280-5cdd-4c88-afee-c0e1002be521
600
Stern, Michael
9883cbf3-4ea3-4e42-8d0b-a47403a01d51
600
Bicker, Gerd
4d9b8bc7-4ad0-457d-adbb-5ad83279d235
600
2016-06-13T15:13:59Z
2016-06-13T15:13:59Z
2012-07-19
Eickhoff, Rene; Lorbeer, Raoul-Amadeus; Scheiblich, Hannah; Heisterkamp, Alexander; Meyer, Heiko et al.: Scanning Laser Optical Tomography Resolves Structural Plasticity during Regeneration in an Insect Brain. In: PloS ONE 7 (2012), Nr. 7, e41236. DOI: http://dx.doi.org/10.1371/journal.pone.0041236
http://www.repo.uni-hannover.de/handle/123456789/314
http://dx.doi.org/10.15488/292
Background: Optical Projection Tomography (OPT) is a microscopic technique that generates three dimensional images from whole mount samples the size of which exceeds the maximum focal depth of confocal laser scanning microscopes. As an advancement of conventional emission-OPT, Scanning Laser Optical Tomography (SLOTy) allows simultaneous detection of fluorescence and absorbance with high sensitivity. In the present study, we employ SLOTy in a paradigm of brain plasticity in an insect model system. Methodology: We visualize and quantify volumetric changes in sensory information procession centers in the adult locust, Locusta migratoria. Olfactory receptor neurons, which project from the antenna into the brain, are axotomized by crushing the antennal nerve or ablating the entire antenna. We follow the resulting degeneration and regeneration in the olfactory centers (antennal lobes and mushroom bodies) by measuring their size in reconstructed SLOTy images with respect to the untreated control side. Within three weeks post treatment antennal lobes with ablated antennae lose as much as 60% of their initial volume. In contrast, antennal lobes with crushed antennal nerves initially shrink as well, but regain size back to normal within three weeks. The combined application of transmission-and fluorescence projections of Neurobiotin labeled axotomized fibers confirms that recovery of normal size is restored by regenerated afferents. Remarkably, SLOTy images reveal that degeneration of olfactory receptor axons has a trans-synaptic effect on second order brain centers and leads to size reduction of the mushroom body calyx. Conclusions: This study demonstrates that SLOTy is a suitable method for rapid screening of volumetric plasticity in insect brains and suggests its application also to vertebrate preparations.
Made available in DSpace on 2016-06-13T15:13:59Z (GMT). No. of bitstreams: 0
Previous issue date: 2012-07-19
DFG/REBIRTH
publishedVersion
eng
San Francisco : Public Library Science
PLoS ONE 7 (2012), Nr. 7
1932-6203
http://dx.doi.org/10.1371/journal.pone.0041236
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
projection tomography
schistocerca-gregaria
gene-expression
mushroom bodies
axonal regeneration
locusta-migratoria
tympanal nerve
desert locust
antennal lobe
microscopy
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::510 | Mathematik
510
600
Dewey Decimal Classification::500 | Naturwissenschaften::530 | Physik
530
600
Scanning Laser Optical Tomography Resolves Structural Plasticity during Regeneration in an Insect Brain
Article
Text
7
7
e41236
openAccess
ORIGINAL
journal.pone.0041236.pdf
journal.pone.0041236.pdf
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123456789/314
oai:www.repo.uni-hannover.de:123456789/314
2022-12-02 16:17:13.905
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/3162022-12-02T16:11:41Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessddc:590status-type:publishedVersionddc:570
Popa-Lisseanu, Ana G.
8fafafd7-0424-4804-aeb8-bedafb940411
Soergel, Karin
e933c99b-2421-40d7-95bd-f9302e4b7195
Luckner, Anja
55d43115-733e-449c-8bc2-eafef508088e
Wassenaar, Leonard I.
b91c738c-8ea0-4b5c-b17d-08b274c2e413
Ibanez, Carlos
561ee10a-0547-4c8b-a523-240373e7e693
Kramer-Schadt, Stephanie
535ab690-5c8e-4374-bc87-5410f7dc6521
Ciechanowski, Mateusz
c816187c-bfd4-4906-b446-63c9e663333c
Goerfoel, Tamas
49d8464f-3bdd-4450-bfc9-565252eea203
Niermann, Ivo
a99d4507-2744-4bf4-8078-f55efd5733e0
Beuneux, Gregory
1c640a05-af85-405f-980b-cf02f242c917
Myslajek, Robert W.
2b0d2780-712b-4bb0-ab4b-2c4f2c1d1661
Juste, Javier
6984f4b0-bf6f-4b3c-8420-8c1e6e5f5c15
Fonderflick, Jocelyn
ca5ec15c-829c-4ed7-a58d-c504183654f6
Kelm, Detlev H.
39e1e149-7781-41cf-8229-b2b9f82ac88a
Voigt, Christian C.
0fdbd4c2-246d-43ca-90fa-890e848d2316
2016-06-13T15:14:00Z
2016-06-13T15:14:00Z
2012-01-23
Popa-Lisseanu, Ana G.; Soergel, Karin; Luckner, Anja; Wassenaar, Leonard I.; Ibanez, Carlos et al.: A Triple-Isotope Approach to Predict the Breeding Origins of European Bats. In: PloS ONE 7 (2012), Nr. 1, e30388. DOI: http://dx.doi.org/10.1371/journal.pone.0030388
http://www.repo.uni-hannover.de/handle/123456789/316
http://dx.doi.org/10.15488/294
Despite a commitment by the European Union to protect its migratory bat populations, conservation efforts are hindered by a poor understanding of bat migratory strategies and connectivity between breeding and wintering grounds. Traditional methods like mark-recapture are ineffective to study broad-scale bat migratory patterns. Stable hydrogen isotopes (delta D) have been proven useful in establishing spatial migratory connectivity of animal populations. Before applying this tool, the method was calibrated using bat samples of known origin. Here we established the potential of delta D as a robust geographical tracer of breeding origins of European bats by measuring delta D in hair of five sedentary bat species from 45 locations throughout Europe. The delta D of bat hair strongly correlated with well-established spatial isotopic patterns in mean annual precipitation in Europe, and therefore was highly correlated with latitude. We calculated a linear mixed-effects model, with species as random effect, linking delta D of bat hair to precipitation delta D of the areas of hair growth. This model can be used to predict breeding origins of European migrating bats. We used delta C-13 and delta N-15 to discriminate among potential origins of bats, and found that these isotopes can be used as variables to further refine origin predictions. A triple-isotope approach could thereby pinpoint populations or subpopulations that have distinct origins. Our results further corroborated stable isotope analysis as a powerful method to delineate animal migrations in Europe.
Made available in DSpace on 2016-06-13T15:14:00Z (GMT). No. of bitstreams: 0
Previous issue date: 2012-01-23
Alexander von Humboldt Foundation
publishedVersion
eng
San Francisco : Public Library Science
PLoS ONE 7 (2012), Nr. 1
1932-6203
http://dx.doi.org/10.1371/journal.pone.0030388
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
stable-isotopes
migratory connectivity
delta-d
north-america
monarch butterflies
natal origins
lesser scaup
hydrogen
ratios
birds
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::590 | Tiere (Zoologie)
590
600
Dewey Decimal Classification::500 | Naturwissenschaften::551 | Geologie, Hydrologie, Meteorologie
551
600
A Triple-Isotope Approach to Predict the Breeding Origins of European Bats
Article
Text
1
7
e30388
openAccess
ORIGINAL
journal.pone.0030388-1.pdf
journal.pone.0030388-1.pdf
application/pdf
389220
https://www.repo.uni-hannover.de/bitstream/123456789/316/1/journal.pone.0030388-1.pdf
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oai:www.repo.uni-hannover.de:123456789/316
2022-12-02 17:11:41.457
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/3172022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessddc:590status-type:publishedVersionddc:570
Jaramillo, Juliana
7e2c995f-65fa-4f3e-9c18-368bb37d67cf
600
Muchugu, Eric
a919135f-2dc6-4b46-9fa9-33da72d24b9d
600
Vega, Fernando E.
5b67cd35-8fa3-48e3-a899-8af3d586ddc0
600
Davis, Aaron
c864334b-fb5b-47c3-bec9-4815c36e2c32
600
Borgemeister, Christian
d1d1adef-7699-4ddd-95ca-291e0d54dbc6
600
Chabi-Olaye, Adenirin
ce55bf6a-9db9-4700-8198-c85894567f64
600
2016-06-13T15:14:00Z
2016-06-13T15:14:00Z
2011-09-14
Jaramillo, Juliana; Muchugu, Eric; Vega, Fernando E.; Davis, Aaron; Borgemeister, Christian; Chabi-Olaye, Adenirin: Some Like It Hot: The Influence and Implications of Climate Change on Coffee Berry Borer (Hypothenemus hampei) and Coffee Production in East Africa. In: PloS ONE 6 (2011), Nr. 9, e24528. DOI: http://dx.doi.org/10.1371/journal.pone.0024528
http://www.repo.uni-hannover.de/handle/123456789/317
http://dx.doi.org/10.15488/295
The negative effects of climate change are already evident for many of the 25 million coffee farmers across the tropics and the 90 billion dollar (US) coffee industry. The coffee berry borer (Hypothenemus hampei), the most important pest of coffee worldwide, has already benefited from the temperature rise in East Africa: increased damage to coffee crops and expansion in its distribution range have been reported. In order to anticipate threats and prioritize management actions for H. hampei we present here, maps on future distributions of H. hampei in coffee producing areas of East Africa. Using the CLIMEX model we relate present-day insect distributions to current climate and then project the fitted climatic envelopes under future scenarios A2A and B2B (for HADCM3 model). In both scenarios, the situation with H. hampei is forecasted to worsen in the current Coffea arabica producing areas of Ethiopia, the Ugandan part of the Lake Victoria and Mt. Elgon regions, Mt. Kenya and the Kenyan side of Mt. Elgon, and most of Rwanda and Burundi. The calculated hypothetical number of generations per year of H. hampei is predicted to increase in all C. arabica-producing areas from five to ten. These outcomes will have serious implications for C. arabica production and livelihoods in East Africa. We suggest that the best way to adapt to a rise of temperatures in coffee plantations could be via the introduction of shade trees in sun grown plantations. The aims of this study are to fill knowledge gaps existing in the coffee industry, and to draft an outline for the development of an adaptation strategy package for climate change on coffee production. An abstract in Spanish is provided as Abstract S1.
Made available in DSpace on 2016-06-13T15:14:00Z (GMT). No. of bitstreams: 0
Previous issue date: 2011-09-14
DFG
publishedVersion
eng
San Francisco : Public Library Science
PLoS ONE 6 (2011), Nr. 9
1932-6203
http://dx.doi.org/10.1371/journal.pone.0024528
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
insect herbivores
impacts
biodiversity
diversity
pests
agriculture
scolytidae
prediction
coleoptera
management
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::590 | Tiere (Zoologie)
590
600
Some Like It Hot: The Influence and Implications of Climate Change on Coffee Berry Borer (Hypothenemus hampei) and Coffee Production in East Africa
Article
Text
9
6
e24528
openAccess
ORIGINAL
journal.pone.0024528.pdf
journal.pone.0024528.pdf
application/pdf
1671477
https://www.repo.uni-hannover.de/bitstream/123456789/317/1/journal.pone.0024528.pdf
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1
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MD5
2
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MD5
3
123456789/317
oai:www.repo.uni-hannover.de:123456789/317
2022-12-02 17:14:10.802
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/3182022-12-02T16:11:40Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessddc:590status-type:publishedVersionddc:570
Wendt, Hanna
67ca7010-4ba7-489d-ab60-e317ac37774e
600
Hillmer, Anja
63dcb5a1-b107-4f28-8c25-0c220bf8d800
600
Reimers, Kerstin
674ae86c-5aba-4df4-818b-34f213563eb0
600
Kuhbier, Jörn W.
0bc26f8b-631f-4766-87a9-365d750a878d
600
Schaefer-Nolte, Franziska
aaa31d6c-7e90-4816-8c75-55dbef02d4b6
600
Allmeling, Christina
490ac287-1145-4b78-91c1-e7ad4ded52a8
600
Kasper, Cornelia
8b273750-8fa6-4393-9c1e-b3d7ff3bddee
600
Vogt, Peter M.
b352d396-ef6a-4277-953e-669fdc0bc30e
600
2016-06-13T15:14:01Z
2016-06-13T15:14:01Z
2011-07-26
Wendt, Hanna; Hillmer, Anja; Reimers, Kerstin; Kuhbier, Joern W.; Schaefer-Nolte, Franziska et al.: Artificial Skin - Culturing of Different Skin Cell Lines for Generating an Artificial Skin Substitute on Cross-Weaved Spider Silk Fibres. In: PloS ONE 6 (2011), Nr. 7, e21833. DOI: http://dx.doi.org/10.1371/journal.pone.0021833
http://www.repo.uni-hannover.de/handle/123456789/318
http://dx.doi.org/10.15488/296
Background: In the field of Plastic Reconstructive Surgery the development of new innovative matrices for skin repair is in urgent need. The ideal biomaterial should promote attachment, proliferation and growth of cells. Additionally, it should degrade in an appropriate time period without releasing harmful substances, but not exert a pathological immune response. Spider dragline silk from Nephila spp meets these demands to a large extent. Methodology/Principal Findings: Native spider dragline silk, harvested directly out of Nephila spp spiders, was woven on steel frames. Constructs were sterilized and seeded with fibroblasts. After two weeks of cultivating single fibroblasts, keratinocytes were added to generate a bilayered skin model, consisting of dermis and epidermis equivalents. For the next three weeks, constructs in co-culture were lifted on an originally designed setup for air/liquid interface cultivation. After the culturing period, constructs were embedded in paraffin with an especially developed program for spidersilk to avoid supercontraction. Paraffin cross-sections were stained in Haematoxylin & Eosin (H&E) for microscopic analyses. Conclusion/Significance: Native spider dragline silk woven on steel frames provides a suitable matrix for 3 dimensional skin cell culturing. Both fibroblasts and keratinocytes cell lines adhere to the spider silk fibres and proliferate. Guided by the spider silk fibres, they sprout into the meshes and reach confluence in at most one week. A well-balanced, bilayered cocultivation in two continuously separated strata can be achieved by serum reduction, changing the medium conditions and the cultivation period at the air/liquid interphase. Therefore spider silk appears to be a promising biomaterial for the enhancement of skin regeneration.
Made available in DSpace on 2016-06-13T15:14:01Z (GMT). No. of bitstreams: 0
Previous issue date: 2011-07-26
publishedVersion
eng
San Francisco : Public Library Science
PLoS ONE 6 (2011), Nr. 7
1932-6203
http://dx.doi.org/10.1371/journal.pone.0021833
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
nephila-clavipes araneae
dragline silk
escherichia-coli
bombyx-mori
in-vivo
biomaterials
scaffolds
protein
web
degradation
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::590 | Tiere (Zoologie)
590
600
Artificial Skin - Culturing of Different Skin Cell Lines for Generating an Artificial Skin Substitute on Cross-Weaved Spider Silk Fibres
Article
Text
7
6
e21833
openAccess
ORIGINAL
journal.pone.0021833.pdf
journal.pone.0021833.pdf
application/pdf
1350114
https://www.repo.uni-hannover.de/bitstream/123456789/318/1/journal.pone.0021833.pdf
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MD5
1
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journal.pone.0021833.pdf.txt
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https://www.repo.uni-hannover.de/bitstream/123456789/318/2/journal.pone.0021833.pdf.txt
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MD5
3
123456789/318
oai:www.repo.uni-hannover.de:123456789/318
2022-12-02 17:11:40.857
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/3192022-12-02T16:11:41Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessddc:590status-type:publishedVersionddc:570
Kuhbier, Jörn W.
0bc26f8b-631f-4766-87a9-365d750a878d
600
Allmeling, Christina
490ac287-1145-4b78-91c1-e7ad4ded52a8
600
Reimers, Kerstin
674ae86c-5aba-4df4-818b-34f213563eb0
600
Hillmer, Anja
63dcb5a1-b107-4f28-8c25-0c220bf8d800
600
Kasper, Cornelia
8b273750-8fa6-4393-9c1e-b3d7ff3bddee
600
Menger, Bjoern
91832ccc-983a-4e60-8539-de9e825b2691
600
Brandes, Gudrun
efde76ce-e994-489a-895a-3131b6f1abf6
600
Guggenheim, Merlin
7d37045b-1a5b-425f-bf0e-56ea97faf4b7
600
Vogt, Peter M.
b352d396-ef6a-4277-953e-669fdc0bc30e
600
2016-06-13T15:14:01Z
2016-06-13T15:14:01Z
2010-08-09
Kuhbier, Joern W.; Allmeling, Christina; Reimers, Kerstin; Hillmer, Anja; Kasper, Cornelia et al.: Interactions between Spider Silk and Cells - NIH/3T3 Fibroblasts Seeded on Miniature Weaving Frames. In: PloS ONE 5 (2010), Nr. 8, e12032. DOI: http://dx.doi.org/10.1371/journal.pone.0012032
http://www.repo.uni-hannover.de/handle/123456789/319
http://dx.doi.org/10.15488/297
Background: Several materials have been used for tissue engineering purposes, since the ideal matrix depends on the desired tissue. Silk biomaterials have come to focus due to their great mechanical properties. As untreated silkworm silk has been found to be quite immunogenic, an alternative could be spider silk. Not only does it own unique mechanical properties, its biocompatibility has been shown already in vivo. In our study, we used native spider dragline silk which is known as the strongest fibre in nature. Methodology/Principal Findings: Steel frames were originally designed and manufactured and woven with spider silk, harvesting dragline silk directly out of the animal. After sterilization, scaffolds were seeded with fibroblasts to analyse cell proliferation and adhesion. Analysis of cell morphology and actin filament alignment clearly revealed adherence. Proliferation was measured by cell count as well as determination of relative fluorescence each after 1, 2, 3, and 5 days. Cell counts for native spider silk were also compared with those for trypsin-digested spider silk. Spider silk specimens displayed less proliferation than collagen-and fibronectin-coated cover slips, enzymatic treatment reduced adhesion and proliferation rates tendentially though not significantly. Nevertheless, proliferation could be proven with high significance (p<0.01). Conclusion/Significance: Native spider silk does not require any modification to its application as a biomaterial that can rival any artificial material in terms of cell growth promoting properties. We could show adhesion mechanics on intracellular level. Additionally, proliferation kinetics were higher than in enzymatically digested controls, indicating that spider silk does not require modification. Recent findings concerning reduction of cell proliferation after exposure could not be met. As biotechnological production of the hierarchical composition of native spider silk fibres is still a challenge, our study has a pioneer role in researching cellular mechanics on native spider silk fibres.
Made available in DSpace on 2016-06-13T15:14:01Z (GMT). No. of bitstreams: 0
Previous issue date: 2010-08-09
Braukmann-Wittenberg Foundation
publishedVersion
eng
San Francisco : Public Library Science
PLoS ONE 5 (2010), Nr. 8
1932-6203
http://dx.doi.org/10.1371/journal.pone.0012032
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
peripheral-nerve regeneration
clavipes dragline silk
nephila-clavipes
in-vitro
fibroin
scaffolds
fibers
proteins
proliferation
biomaterials
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::590 | Tiere (Zoologie)
590
600
Interactions between Spider Silk and Cells - NIH/3T3 Fibroblasts Seeded on Miniature Weaving Frames
Article
Text
8
5
e12032
openAccess
ORIGINAL
journal.pone.0012032.pdf
journal.pone.0012032.pdf
application/pdf
2102099
https://www.repo.uni-hannover.de/bitstream/123456789/319/1/journal.pone.0012032.pdf
7d8d19154abd375f238640bc56a6e607
MD5
1
TEXT
journal.pone.0012032.pdf.txt
journal.pone.0012032.pdf.txt
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46928
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MD5
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MD5
3
123456789/319
oai:www.repo.uni-hannover.de:123456789/319
2022-12-02 17:11:41.501
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/3202022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Jaramillo, Juliana
7e2c995f-65fa-4f3e-9c18-368bb37d67cf
600
Chabi-Olaye, Adenirin
ce55bf6a-9db9-4700-8198-c85894567f64
600
Kamonjo, Charles
f5efdbb0-d681-4ab7-b90f-48b8070b18fb
600
Jaramillo, Alvaro
a51776da-e038-4719-a9bd-82c5b290e2d5
600
Vega, Fernando E.
5b67cd35-8fa3-48e3-a899-8af3d586ddc0
600
Poehling, Hans-Michael
84b8da68-9ed7-4a7f-a98c-29ba64f9092c
600
Borgemeister, Christian
d1d1adef-7699-4ddd-95ca-291e0d54dbc6
600
2016-06-13T15:14:01Z
2016-06-13T15:14:01Z
2009-08-03
Jaramillo, Juliana; Chabi-Olaye, Adenirin; Kamonjo, Charles; Jaramillo, Alvaro; Vega, Fernando E. et al.: Thermal Tolerance of the Coffee Berry Borer Hypothenemus hampei: Predictions of Climate Change Impact on a Tropical Insect Pest. In: PloS ONE 4 (2009), Nr. 8, e6487. DOI: http://dx.doi.org/10.1371/journal.pone.0006487
http://www.repo.uni-hannover.de/handle/123456789/320
http://dx.doi.org/10.15488/298
Coffee is predicted to be severely affected by climate change. We determined the thermal tolerance of the coffee berry borer, Hypothenemus hampei, the most devastating pest of coffee worldwide, and make inferences on the possible effects of climate change using climatic data from Colombia, Kenya, Tanzania, and Ethiopia. For this, the effect of eight temperature regimes (15, 20, 23, 25, 27, 30, 33 and 35 degrees C) on the bionomics of H. hampei was studied. Successful egg to adult development occurred between 20-30 degrees C. Using linear regression and a modified Logan model, the lower and upper thresholds for development were estimated at 14.9 and 32 degrees C, respectively. In Kenya and Colombia, the number of pest generations per year was considerably and positively correlated with the warming tolerance. Analysing 32 years of climatic data from Jimma (Ethiopia) revealed that before 1984 it was too cold for H. hampei to complete even one generation per year, but thereafter, because of rising temperatures in the area, 1-2 generations per year/coffee season could be completed. Calculated data on warming tolerance and thermal safety margins of H. hampei for the three East African locations showed considerably high variability compared to the Colombian site. The model indicates that for every 1 degrees C rise in thermal optimum (T(opt)), the maximum intrinsic rate of increase (r(max)) will increase by an average of 8.5%. The effects of climate change on the further range of H. hampei distribution and possible adaption strategies are discussed. Abstracts in Spanish and French are provided as supplementary material Abstract S1 and Abstract S2.
Made available in DSpace on 2016-06-13T15:14:01Z (GMT). No. of bitstreams: 0
Previous issue date: 2009-08-03
DFG
publishedVersion
eng
San Francisco : Public Library Science
PLoS ONE 4 (2009), Nr. 8
1932-6203
http://dx.doi.org/10.1371/journal.pone.0006487
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
Berries
climate change
purpae
Ethiopia
Africa
Colombia
larvae
plant-herbivore interactions
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Thermal Tolerance of the Coffee Berry Borer Hypothenemus hampei: Predictions of Climate Change Impact on a Tropical Insect Pest
Article
Text
8
4
e6487
openAccess
ORIGINAL
journal.pone.0006487.pdf
journal.pone.0006487.pdf
application/pdf
328319
https://www.repo.uni-hannover.de/bitstream/123456789/320/1/journal.pone.0006487.pdf
4e7fd87edb344474019c09948935daac
MD5
1
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journal.pone.0006487.pdf.txt
journal.pone.0006487.pdf.txt
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58321
https://www.repo.uni-hannover.de/bitstream/123456789/320/2/journal.pone.0006487.pdf.txt
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MD5
2
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journal.pone.0006487.pdf.jpg
journal.pone.0006487.pdf.jpg
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1812
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MD5
3
123456789/320
oai:www.repo.uni-hannover.de:123456789/320
2022-12-02 17:14:10.355
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/3222022-12-02T16:10:15Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Amien, Suseno
2c0848e5-f1a8-4503-a654-995bba739683
600
Kliwer, Irina
addc17d9-f255-4679-b17d-dcf1ceec2b52
600
Marton, Mihaela L.
77a2b4a7-f438-488e-9b38-c0406799cadd
600
Debener, Thomas
2aedee57-8d8b-49ca-9c67-1a988b8fba75
600
Geiger, Dietmar
fe5188ff-adc5-45af-9294-569a61783757
600
Becker, Dirk
46e8f53a-2a84-4d09-945b-bcd941028247
600
Dresselhaus, Thomas
c329c265-680a-4b5b-bcd2-f45335521933
600
2016-06-13T15:14:01Z
2016-06-13T15:14:01Z
2010-06
Amien, Suseno; Kliwer, Irina; Marton, Mihaela L.; Debener, Thomas; Geiger, Dietmar et al.: Defensin-Like ZmES4 Mediates Pollen Tube Burst in Maize via Opening of the Potassium Channel KZM1. In: PloS Biology 8 (2010), Nr. 6, e1000388. DOI: http://dx.doi.org/10.1371/journal.pbio.1000388
http://www.repo.uni-hannover.de/handle/123456789/322
http://dx.doi.org/10.15488/300
In contrast to animals and lower plant species, sperm cells of flowering plants are non-motile and are transported to the female gametes via the pollen tube, i.e. the male gametophyte. Upon arrival at the female gametophyte two sperm cells are discharged into the receptive synergid cell to execute double fertilization. The first players involved in inter-gametophyte signaling to attract pollen tubes and to arrest their growth have been recently identified. In contrast the physiological mechanisms leading to pollen tube burst and thus sperm discharge remained elusive. Here, we describe the role of polymorphic defensin-like cysteine-rich proteins ZmES1-4 (Zea mays embryo sac) from maize, leading to pollen tube growth arrest, burst, and explosive sperm release. ZmES1-4 genes are exclusively expressed in the cells of the female gametophyte. ZmES4-GFP fusion proteins accumulate in vesicles at the secretory zone of mature synergid cells and are released during the fertilization process. Using RNAi knock-down and synthetic ZmES4 proteins, we found that ZmES4 induces pollen tube burst in a species-preferential manner. Pollen tube plasma membrane depolarization, which occurs immediately after ZmES4 application, as well as channel blocker experiments point to a role of K(+)-influx in the pollen tube rupture mechanism. Finally, we discovered the intrinsic rectifying K(+) channel KZM1 as a direct target of ZmES4. Following ZmES4 application, KZM1 opens at physiological membrane potentials and closes after wash-out. In conclusion, we suggest that vesicles containing ZmES4 are released from the synergid cells upon male-female gametophyte signaling. Subsequent interaction between ZmES4 and KZM1 results in channel opening and K(+) influx. We further suggest that K(+) influx leads to water uptake and culminates in osmotic tube burst. The species-preferential activity of polymorphic ZmES4 indicates that the mechanism described represents a pre-zygotic hybridization barrier and may be a component of reproductive isolation in plants.
Made available in DSpace on 2016-06-13T15:14:01Z (GMT). No. of bitstreams: 0
Previous issue date: 2010-06
DFG/DR334/2-6
DFG/BE1867/4-1
DFG/3976/1-1
German Academic Exchange Service DAAD/A/08/07164
publishedVersion
eng
San Francisco : Public Library Science
PLoS Biology 8 (2010), Nr. 6
1544-9173
http://dx.doi.org/10.1371/journal.pbio.1000388
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
arabidopsis female gametophyte
antimicrobial peptides
scutellar tissue
synergid cells
zea-mays
plant
genes
fertilization
expression
proteins
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Defensin-Like ZmES4 Mediates Pollen Tube Burst in Maize via Opening of the Potassium Channel KZM1
Article
Text
6
8
e1000388
openAccess
ORIGINAL
journal.pbio.1000388.pdf
journal.pbio.1000388.pdf
application/pdf
5162949
https://www.repo.uni-hannover.de/bitstream/123456789/322/1/journal.pbio.1000388.pdf
310f1108390b6089a4c3eebfec046a31
MD5
1
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journal.pbio.1000388.pdf.txt
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78263
https://www.repo.uni-hannover.de/bitstream/123456789/322/2/journal.pbio.1000388.pdf.txt
a605b4ff9e42a076215ec96f8aed3975
MD5
2
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journal.pbio.1000388.pdf.jpg
journal.pbio.1000388.pdf.jpg
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https://www.repo.uni-hannover.de/bitstream/123456789/322/3/journal.pbio.1000388.pdf.jpg
c3f24881a62f1df6bb3b02849b2182e9
MD5
3
123456789/322
oai:www.repo.uni-hannover.de:123456789/322
2022-12-02 17:10:15.455
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/3242022-12-02T18:18:52Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Weiss, Christian
4fcb8696-2f10-4fd3-920f-1ec23e6cfb30
600
Weiss, Joanna
ded865f1-3e3e-4cb7-be0f-c0f1caa4dfb3
600
Boy, Jens
390f6697-9f9a-4d47-8002-11d13db326e2
600
Iskandar, Issi
c31a465e-7b52-4361-b1bc-e088de19cc8a
600
Mikutta, Robert
011194d0-939e-421b-bd3d-cdf0034e53d9
600
Guggenberger, Georg
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2016-06-29T17:09:42Z
2016-06-29T17:09:42Z
2016-06-26
Weiss, Christian; Weiss, Joanna; Boy, Jens; Iskandar, Issi; Mikutta, Robert; Guggenberger, Georg: Soil organic carbon stocks in estuarine and marine mangrove ecosystems are driven by nutrient colimitation of P and N. In: Ecology and Evolution 6 (2016), Nr 14, S. 5043–5056. DOI: https://doi.org/10.1002/ece3.2258
http://www.repo.uni-hannover.de/handle/123456789/324
http://dx.doi.org/10.15488/302
Mangroves play an important role in carbon sequestration, but soil organic carbon (SOC) stocks differ between marine and estuarine mangroves, suggesting differing processes and drivers of SOC accumulation. Here, we compared undegraded and degraded marine and estuarine mangroves in a regional approach across the Indonesian archipelago for their SOC stocks and evaluated possible drivers imposed by nutrient limitations along the land-to-sea gradients. SOC stocks in natural marine mangroves (271–572 Mg ha-1 m-1 were much higher than under estuarine mangroves (100–315 Mg ha-1 m-1 with a further decrease caused by degradation to 80–132 Mg ha-1 m-1. Soils differed in C/N ratio (marine: 29–64; estuarine: 9–28), δ15N (marine: 0.6 to 0.7‰; estuarine: 2.5 to 7.2‰), and plant-available P (marine: 2.3–6.3 mg kg-1; estuarine: 0.16–1.8 mg kg-1). We found N and P supply of sea-oriented mangroves primarily met by dominating symbiotic N2 fixation from air and P import from sea, while mangroves on the landward gradient increasingly covered their demand in N and P from allochthonous sources and SOM recycling. Pioneer plants favored by degradation further increased nutrient recycling from soil resulting in smaller SOC stocks in the topsoil. These processes explained the differences in SOC stocks along the land-to-sea gradient in each mangrove type as well as the SOC stock differences observed between estuarine and marine mangrove ecosystems. This first large-scale evaluation of drivers of SOC stocks under mangroves thus suggests a continuum in mangrove functioning across scales and ecotypes and additionally provides viable proxies for carbon stock estimations in PES or REDD schemes.
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Previous issue date: 2016-06-26
BMBF/03F0644
publishedVersion
eng
New York, NY : Wiley
Ecology and Evolution (2016)
http://dx.doi.org/10.1002/ece3.2258
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Ecosystem functioning
global change
Indonesia
marine and estuarine mangroves
nitrogen
phosphorus
soil organic carbon
stable isotopes
Indonesien
Globaler Wandel
Mangroven
Phosphor
Stickstoff
Organischer Kohlenstoffgehalt
Indonesien
Umweltkrise
Ökosystemforschung
Mangrove
Kohlenstoffgehalt
Kohlenstoffhaushalt
stabiles Isotop
Sequestrierung
Phosphor
Stickstoff
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Soil organic carbon stocks in estuarine and marine mangrove ecosystems are driven by nutrient colimitation of P and N
Article
Text
14
6
5043
5056
openAccess
Ecology and Evolution (2016)
Georg
Robert
Issi
Jens
Joanna
Christian
Guggenberger
Mikutta
Iskandar
Boy
Weiss
Weiss
LUH_Fonds
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oai:www.repo.uni-hannover.de:123456789/5072022-12-02T16:11:40Zcom_123456789_1col_123456789_8doc-type:Articleddc:540doc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Wild, Birgit
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600
Schnecker, Jörg
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Knoltsch, Anna
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600
Takriti, Mounir
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600
Mooshammer, Maria
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Gentsch, Norman
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Mikutta, Robert
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Alves, Ricardo J. Eloy
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Gittel, Antje
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Lashchinskiy, Nikolay
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Richter, Andreas
52d71f9c-5e93-47ae-9872-02fab3c97c0c
600
2016-09-01T09:05:32Z
2016-09-01T09:05:32Z
2015-05
Wild, Birgit; Schnecker, Joerg; Knoltsch, Anna; Takriti, Mounir; Mooshammer, Maria et al.: Microbial nitrogen dynamics in organic and mineral soil horizons along a latitudinal transect in western Siberia. In: Global Biogeochemical Cycles 29 (2015), Nr. 5, S. 567-582. DOI: http://dx.doi.org/10.1002/2015GB005084
http://www.repo.uni-hannover.de/handle/123456789/507
http://dx.doi.org/10.15488/483
Soil N availability is constrained by the breakdown of N-containing polymers such as proteins to oligopeptides and amino acids that can be taken up by plants and microorganisms. Excess N is released from microbial cells as ammonium (N mineralization), which in turn can serve as substrate for nitrification. According to stoichiometric theory, N mineralization and nitrification are expected to increase in relation to protein depolymerization with decreasing N limitation, and thus from higher to lower latitudes and from topsoils to subsoils. To test these hypotheses, we compared gross rates of protein depolymerization, N mineralization and nitrification (determined using N-15 pool dilution assays) in organic topsoil, mineral topsoil, and mineral subsoil of seven ecosystems along a latitudinal transect in western Siberia, from tundra (67 degrees N) to steppe (54 degrees N). The investigated ecosystems differed strongly in N transformation rates, with highest protein depolymerization and N mineralization rates in middle and southern taiga. All N transformation rates decreased with soil depth following the decrease in organic matter content. Related to protein depolymerization, N mineralization and nitrification were significantly higher in mineral than in organic horizons, supporting a decrease in microbial N limitation with depth. In contrast, we did not find indications for a decrease in microbial N limitation from arctic to temperate ecosystems along the transect. Our findings thus challenge the perception of ubiquitous N limitation at high latitudes, but suggest a transition from N to C limitation of microorganisms with soil depth, even in high-latitude systems such as tundra and boreal forest.
Made available in DSpace on 2016-09-01T09:05:32Z (GMT). No. of bitstreams: 0
Previous issue date: 2015-05
Austrian Science Fund/FWF/1370-B17
publishedVersion
eng
Washington : Amer Geophysical Union
Global Biogeochemical Cycles 29 (2015), Nr. 5
1944-9224
0886-6236
http://dx.doi.org/10.1002/2015GB005084
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
tundra
permafrost
boreal forest
protein depolymerization
arctic tundra
terrestrial ecosystems
carbon availability
forest ecosystems
alaskan tundra
use efficiency
plant-growth
n uptake
permafrost
matter
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
540
600
Microbial nitrogen dynamics in organic and mineral soil horizons along a latitudinal transect in western Siberia
Article
Text
5
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2022-12-02 17:11:40.837
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/5112022-12-02T16:14:09Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570ddc:500
Schadzek, Patrik
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600
Schlingmann, Barbara
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600
Schaarschmidt, Frank
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Lindner, Julia
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Koval, Michael
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Heisterkamp, Alexander
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Ngezahayo, Anaclet
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Preller, Matthias
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2016-09-01T09:05:34Z
2016-09-01T09:05:34Z
2016
Schadzek, Patrik; Schlingmann, Barbara; Schaarschmidt, Frank; Lindner, Julia; Koval, Michael et al.: Data of the molecular dynamics simulations of mutations in the human connexin46 docking interface. In: Data in Brief 7 (2016), S. 93-99. DOI: http://dx.doi.org/10.1016/j.dib.2016.01.067
http://www.repo.uni-hannover.de/handle/123456789/511
http://dx.doi.org/10.15488/487
The structure of hCx26 derived from the X-ray analysis was used to generate a homology model for hCx46. Interacting connexin molecules were used as starting model for the molecular dynamics (MD) simulation using NAMD and allowed us to predict the dynamic behavior of hCx46wt and the cataract related mutant hCx46N188T as well as two artificial mutants hCx46N188Q and hCx46N188D. Within the 50 ns simulation time the docked complex composed of the mutants dissociate while hCx46wt remains stable. The data indicates that one hCx46 molecule forms 5-7 hydrogen bonds (HBs) with the counterpart connexin of the opposing connexon. These HBs appear essential for a stable docking of the connexons as shown by the simulation of an entire gap junction channel and were lost for all the tested mutants. The data described here are related to the research article entitled "The cataract related mutation N188T in human connexin46 (hCx46) revealed a critical role for residue N188 in the docking process of gap junction channels" (Schadzek et al., 2015) [1].
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Amsterdam : Elsevier
Data in Brief 7 (2016)
http://dx.doi.org/10.1016/j.dib.2016.01.067
2352-3409
http://dx.doi.org/10.1016/j.dib.2016.01.067
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Cataract
Connexin
Dye transfer
Hemichannel docking
Molecular dynamics
Structural modeling
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
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Data of the molecular dynamics simulations of mutations in the human connexin46 docking interface
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https://www.repo.uni-hannover.de/bitstream/123456789/511/3/1-s2.0-S2352340916300191-main.pdf.jpg
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MD5
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123456789/511
oai:www.repo.uni-hannover.de:123456789/511
2022-12-02 17:14:09.598
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/5152022-12-02T15:17:14Zcom_123456789_1col_123456789_7doc-type:Articleddc:540doc-type:Textopen_accessddc:530status-type:publishedVersionddc:570ddc:500
Peise, Jan
ae56712d-5859-40d2-ac4b-f79b8ff24c69
600
Kruse, I.
96955f11-dd76-445b-9161-5a75334fb215
600
Lange, K.
e0166f29-97ac-426d-bc6e-65559ec60242
600
Lücke, Bernd
19e9c8c2-0813-49dc-8752-27b8c8b227ec
600
Pezze, L.
764c38ed-bd73-40e5-b158-e9b2e04e03ea
600
Arlt, Jan J.
81307136-be7c-4b69-8239-b4890cce33c4
600
Ertmer, Wolfgang
ad414cbe-4a07-4dc5-ae34-4007e837f95f
600
Hammerer, Klemens
d9893553-b8c1-46f4-b939-4eb197b62f5a
600
Santos, Luis
33c918eb-8158-4a1a-a4c3-cb993500dad3
600
Smerzi, A.
7ccad446-33d3-4794-8d8c-e2bc5e49d1ee
600
Klempt, Carsten
41042b0c-a858-4fd5-a30e-6bd1894b17a5
600
2016-09-01T09:05:35Z
2016-09-01T09:05:35Z
2015
Peise, J.; Kruse, I.; Lange, K.; Lucke, B.; Pezze, L. et al.: Satisfying the Einstein-Podolsky-Rosen criterion with massive particles. In: Nature Communications 6 (2015), 8984. DOI: http://dx.doi.org/10.1038/ncomms9984
http://www.repo.uni-hannover.de/handle/123456789/515
http://dx.doi.org/10.15488/491
In 1935, Einstein, Podolsky and Rosen (EPR) questioned the completeness of quantum mechanics by devising a quantum state of two massive particles with maximally correlated space and momentum coordinates. The EPR criterion qualifies such continuous-variable entangled states, where a measurement of one subsystem seemingly allows for a prediction of the second subsystem beyond the Heisenberg uncertainty relation. Up to now, continuous-variable EPR correlations have only been created with photons, while the demonstration of such strongly correlated states with massive particles is still outstanding. Here we report on the creation of an EPR-correlated two-mode squeezed state in an ultracold atomic ensemble. The state shows an EPR entanglement parameter of 0.18(3), which is 2.4 s.d. below the threshold 1/4 of the EPR criterion. We also present a full tomographic reconstruction of the underlying many-particle quantum state. The state presents a resource for tests of quantum nonlocality and a wide variety of applications in the field of continuous-variable quantum information and metrology.
Made available in DSpace on 2016-09-01T09:05:35Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
QUEST
DFG/Research Training Group/1729
European Metrology Research Programme
publishedVersion
eng
London : Macmillan Publishers Limited
Nature Communications 6 (2015)
2041-1723
http://dx.doi.org/10.1038/ncomms9984
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Physical sciences
Atomic and molecular physics
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
540
600
Dewey Decimal Classification::500 | Naturwissenschaften::530 | Physik
530
600
Satisfying the Einstein-Podolsky-Rosen criterion with massive particles
Article
Text
6
8984
openAccess
ORIGINAL
ncomms9984.pdf
ncomms9984.pdf
application/pdf
2487256
https://www.repo.uni-hannover.de/bitstream/123456789/515/1/ncomms9984.pdf
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MD5
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ncomms9984.pdf.txt
ncomms9984.pdf.txt
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MD5
2
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ncomms9984.pdf.jpg
ncomms9984.pdf.jpg
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MD5
3
123456789/515
oai:www.repo.uni-hannover.de:123456789/515
2022-12-02 16:17:14.209
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/5172022-12-02T16:11:40Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Nyasani, Johnson O.
3eccb723-67f2-4c06-887e-97fd38e7fab8
600
Subramanian, Sevgan
f28faf84-1528-4c04-880e-02da22e40a44
600
Poehling, Hans-Michael
84b8da68-9ed7-4a7f-a98c-29ba64f9092c
600
Maniania, Nguya K.
ae520cfc-3965-4bb7-8340-dd3a17ebd231
600
Ekesi, Sunday
bbd7feed-e359-42b1-8c60-1acd1c630021
600
Meyhöfer, Rainer
e779ffa5-b734-466d-bd85-c13de94b68ec
600
2016-09-01T09:05:36Z
2016-09-01T09:05:36Z
2015
Nyasani, Johnson O.; Subramanian, Sevgan; Poehling, Hans-Michael; Maniania, Nguya K.; Ekesi, Sunday; Meyhofer, Rainer: Optimizing Western Flower Thrips Management on French Beans by Combined Use of Beneficials and Imidacloprid. In: Insects 6 (2015), Nr. 1, S. 279-296. DOI: http://dx.doi.org/10.3390/insects6010279
http://www.repo.uni-hannover.de/handle/123456789/517
http://dx.doi.org/10.15488/493
Western flower thrips (WFT), Frankliniella occidentalis (Pergande), is an important pest of vegetable crops worldwide and has developed resistance to many insecticides. The predatory mites Neoseiulus (=Amblyseius) cucumeris (Oudemans), the entomopathogenic fungus Metarhizium anisopliae (Metsch.), and an insecticide (imidacloprid) were tested for their efficacy to reduce WFT population density and damage to French bean (Phaseolus vulgaris L.) pods under field conditions in two planting periods. Metarhizium anisopliae was applied as a foliar spray weekly at a rate of one litre spray volume per plot while imidacloprid was applied as a soil drench every two weeks at a rate of two litres of a mixture of water and imidacloprid per m(2). Neoseiulus cucumeris was released every two weeks on plant foliage at a rate of three mites per plant. Single and combined treatment applications reduced WFT population density by at least three times and WFT damage to French bean pods by at least 1.7 times compared with untreated plots. The benefit-cost ratios in management of WFT were profitable with highest returns realized on imidacloprid treated plots. The results indicate that M. anisopliae, N. cucumeris, and imidacloprid have the potential for use in developing an integrated pest management program against WFT on French beans.
Made available in DSpace on 2016-09-01T09:05:36Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
BMZ/GIZ/07.7860.5-001.00
publishedVersion
eng
Basel : Mdpi Ag
Insects 6 (2015), Nr. 1
2075-4450
2075-4450
http://dx.doi.org/10.3390/insects6010279
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Amblyseius
benefit-cost ratio
Frankliniella occidentalis
entomopathogenic fungus
neonicotinoid
Phaseolus vulgaris
predatory mite
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Optimizing Western Flower Thrips Management on French Beans by Combined Use of Beneficials and Imidacloprid
Article
Text
1
6
279
296
openAccess
ORIGINAL
insects-06-00279.pdf
insects-06-00279.pdf
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https://www.repo.uni-hannover.de/bitstream/123456789/517/1/insects-06-00279.pdf
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MD5
1
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insects-06-00279.pdf.txt
insects-06-00279.pdf.txt
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51412
https://www.repo.uni-hannover.de/bitstream/123456789/517/2/insects-06-00279.pdf.txt
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MD5
2
THUMBNAIL
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image/jpeg
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MD5
3
123456789/517
oai:www.repo.uni-hannover.de:123456789/517
2022-12-02 17:11:40.824
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/5192022-12-02T15:12:31Zcom_123456789_1col_123456789_7doc-type:Articleddc:540doc-type:Textopen_accessddc:530status-type:publishedVersionddc:570ddc:500
Abdelkhalek, Daniela
6b0485b9-0f56-4c82-9a6e-e1de0334250a
600
Syllwasschy, Mareike
4a22f697-9bdf-4c9b-8f8b-d0268919b2d8
600
Cerf, Nicolas J.
3628868f-c799-4d91-89d4-27519993fb60
600
Fiurasek, Jaromir
6435d47c-7811-4fbb-a41c-799f4c259ab2
600
Schnabel, Roman
7c8d859f-0d3e-4053-ab12-7a8eea7f2fb7
600
2016-09-01T09:06:11Z
2016-09-01T09:06:11Z
2016
Abdelkhalek, Daniela; Syllwasschy, Mareike; Cerf, Nicolas J.; Fiurasek, Jaromir; Schnabel, Roman: Efficient entanglement distillation without quantum memory. In: Nature Communications 7 (2016), 11720. DOI: http://dx.doi.org/10.1038/ncomms11720
http://www.repo.uni-hannover.de/handle/123456789/519
http://dx.doi.org/10.15488/495
Entanglement distribution between distant parties is an essential component to most quantum communication protocols. Unfortunately, decoherence effects such as phase noise in optical fibres are known to demolish entanglement. Iterative (multistep) entanglement distillation protocols have long been proposed to overcome decoherence, but their probabilistic nature makes them inefficient since the success probability decays exponentially with the number of steps. Quantum memories have been contemplated to make entanglement distillation practical, but suitable quantum memories are not realised to date. Here, we present the theory for an efficient iterative entanglement distillation protocol without quantum memories and provide a proof-of-principle experimental demonstration. The scheme is applied to phase-diffused two-mode-squeezed states and proven to distil entanglement for up to three iteration steps. The data are indistinguishable from those that an efficient scheme using quantum memories would produce. Since our protocol includes the final measurement it is particularly promising for enhancing continuous-variable quantum key distribution.
Made available in DSpace on 2016-09-01T09:06:11Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
DFG/SCHN 757/5-1
EU/FP7/308803
EU/FP7/7E13032
FNRS/T.0199.13
BELSPO/IAP P7-35
DFG/RTG1991
publishedVersion
eng
London : Nature Publishing Group
Nature Communications 7 (2016)
2041-1723
http://dx.doi.org/10.1038/ncomms11720
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Physical sciences
Theoretical physics
Applied physics
Optical physics
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
540
600
Dewey Decimal Classification::500 | Naturwissenschaften::530 | Physik
530
600
Efficient entanglement distillation without quantum memory
Article
Text
7
11720
openAccess
ORIGINAL
ncomms11720.pdf
ncomms11720.pdf
application/pdf
903832
https://www.repo.uni-hannover.de/bitstream/123456789/519/1/ncomms11720.pdf
bbbe59b4e00692525e032a3f1eb887f0
MD5
1
TEXT
ncomms11720.pdf.txt
ncomms11720.pdf.txt
Extracted Text
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41856
https://www.repo.uni-hannover.de/bitstream/123456789/519/2/ncomms11720.pdf.txt
9dd0466b5ef9241bc1e410480965eb58
MD5
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ncomms11720.pdf.jpg
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MD5
3
123456789/519
oai:www.repo.uni-hannover.de:123456789/519
2022-12-02 16:12:31.875
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/5242022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
McCaughey, Janine
426ebe0e-b276-4a57-a12d-772d79c06d61
-1
Miller, Victoria J.
b4dfb42a-d531-4c8d-a747-2cd8d8ed35f1
-1
Stevenson, Nicola L.
77cf980d-71ae-4232-a9f0-cf792bf04ec3
-1
Brown, Anna K.
2aadffa0-7752-4c83-8bd2-c319367da141
-1
Budnik, Annika
d57f63dc-8ec2-4c70-8ad3-07b1ae65bc00
-1
Heesom, Kate J.
2a46b28a-a94d-4447-b47f-6b61e9e20876
-1
Alibhai, Dominic
5ebcc4c7-09c5-4a69-b0bb-7a9018983b6a
-1
Stephens, David J.
0f0d5ed5-7322-47b0-954b-ce7d85070455
-1
2016-09-02T08:00:21Z
2016-09-02T08:00:21Z
2016
McCaughey, Janine; Miller, Victoria J.; Stevenson, Nicola L.; Brown, Anna K.; Budnik, Annika et al.: TFG Promotes Organization of Transitional ER and Efficient Collagen Secretion. In: Cell Reports 15 (2016), Nr. 8, S. 1648-1659. DOI: http://dx.doi.org/10.1016/j.celrep.2016.04.062
http://www.repo.uni-hannover.de/handle/123456789/524
http://dx.doi.org/10.15488/500
Collagen is the most abundant protein in the animal kingdom. It is of fundamental importance during development for cell differentiation and tissue morphogenesis as well as in pathological processes such as fibrosis and cancer cell migration. However, our understanding of the mechanisms of procollagen secretion remains limited. Here, we show that TFG organizes transitional ER (tER) and ER exit sites (ERESs) into larger structures. Depletion of TFG results in dispersion of tER elements that remain associated with individual ER-Golgi intermediate compartments (ERGICs) as largely functional ERESs. We show that TFG is not required for the transport and packaging of small soluble cargoes but is necessary for the export of procollagen from the ER. Our work therefore suggests a key relationship between the structure and function of ERESs and a central role for TFG in optimizing COPII assembly for procollagen export.
Made available in DSpace on 2016-09-02T08:00:21Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
Medical Research Council UK/MR/J000604/1
Medical Research Council UK/MR/K018019/1
Medical Research Council UK/MR/G0801848
publishedVersion
eng
Amsterdam : Elsevier
Cell Reports 15 (2016), Nr. 8
2211-1247
http://dx.doi.org/10.1016/j.celrep.2016.04.062
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
TFG
collagen
ERESs
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
TFG Promotes Organization of Transitional ER and Efficient Collagen Secretion
Article
Text
8
15
1648
1659
openAccess
ORIGINAL
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1-s2.0-S221112471630506X-main.pdf
application/pdf
5123207
https://www.repo.uni-hannover.de/bitstream/123456789/524/1/1-s2.0-S221112471630506X-main.pdf
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MD5
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1-s2.0-S221112471630506X-main.pdf.txt
1-s2.0-S221112471630506X-main.pdf.txt
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text/plain
58092
https://www.repo.uni-hannover.de/bitstream/123456789/524/2/1-s2.0-S221112471630506X-main.pdf.txt
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MD5
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6fb8ab65a96a4bf5c3071af25423de4a
MD5
3
123456789/524
oai:www.repo.uni-hannover.de:123456789/524
2022-12-02 17:14:10.358
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/5722022-12-02T15:04:50Zcom_123456789_1col_123456789_6doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570ddc:600
Richter, Berna I.
b3d3ce3b-4477-4c21-97f6-47f9aa2638c6
600
Ostermeier, Sven
26546781-2c2b-4389-a1e7-ae79958fdccd
600
Turger, Anke
665fc51a-740d-4038-be4d-f6390043f3c7
600
Denkena, Berend
9acacde7-c7cf-4f5a-87fd-2018e746a754
600
Hurschler, Christof
41e19f16-ce24-4b63-86c3-7e2a7555932c
600
2016-10-28T09:05:29Z
2016-10-28T09:05:29Z
2010
Richter, B.I.; Ostermeier, S.; Turger, Anke; Denkena, Berend; Hurschler, C.: A rolling-gliding wear simulator for the investigation of tribological material pairings for application in total knee arthroplasty. In: BioMedical Engineering Online 9 (2010) , 24. DOI: http://dx.doi.org/10.1186/1475-925X-9-24
http://www.repo.uni-hannover.de/handle/123456789/572
http://dx.doi.org/10.15488/548
Background: Material wear testing is an important technique in the development and evaluation of materials for use in implant for total knee arthroplasty. Since a knee joint induces a complex rolling-gliding movement, standardised material wear testing devices such as Pin-on-Disc or Ring-on-Disc testers are suitable to only a limited extent because they generate pure gliding motion only.Methods: A rolling-gliding wear simulator was thus designed, constructed and implemented, which simulates and reproduces the rolling-gliding movement and loading of the knee joint on specimens of simplified geometry. The technical concept was to run a base-plate, representing the tibia plateau, against a pivoted cylindrical counter-body, representing one femur condyle under an axial load. A rolling movement occurs as a result of the friction and pure gliding is induced by limiting the rotation of the cylindrical counter-body. The set up also enables simplified specimens handling and removal for gravimetrical wear measurements. Long-term wear tests and gravimetrical wear measurements were carried out on the well known material pairings: cobalt chrome-polyethylene, ceramic-polyethylene and ceramic-ceramic, over three million motion cycles to allow material comparisons to be made.Results: The observed differences in wear rates between cobalt-chrome on polyethylene and ceramic on polyethylene pairings were similar to the differences of published data for existing material-pairings. Test results on ceramic-ceramic pairings of different frontal-plane geometry and surface roughness displayed low wear rates and no fracture failures.Conclusions: The presented set up is able to simulate the rolling-gliding movement of the knee joint, is easy to use, and requires a minimum of user intervention or monitoring. It is suitable for long-term testing, and therefore a useful tool for the investigation of new and promising materials which are of interest for application in knee joint replacement implants.
Made available in DSpace on 2016-10-28T09:05:29Z (GMT). No. of bitstreams: 0
Previous issue date: 2010
DFG/Collaborative Research Center 599 for Biomedical Technology
publishedVersion
eng
London : BioMed Central Ltd.
BioMedical Engineering Online 9 (2010)
1475-925X
http://dx.doi.org/10.1186/1475-925X-9-24
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Knee joint
Knee joint replacement
Material wear
Motion cycle
Pin on disc
Plane geometry
Promising materials
Ring-on-disc
Rolling movement
Test results
Total knee arthroplasty
User intervention
Wear measurements
Wear rates
Wear simulators
Wear test
Cobalt
Joint prostheses
Joints (anatomy)
Materials testing
Polyethylenes
Surface roughness
Surgery
Thermoplastics
Ceramic materials
cobalt
polyethylene
article
biological model
ceramics
femur
instrumentation
knee arthroplasty
materials testing
methodology
motion
surface property
tibia
Arthroplasty, Replacement, Knee
Ceramics
Cobalt
Femur
Materials Testing
Models, Biological
Motion
Polyethylene
Surface Properties
Tibia
Dewey Decimal Classification::600 | Technik
600
600
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
A rolling-gliding wear simulator for the investigation of tribological material pairings for application in total knee arthroplasty
Article
Text
9
24
openAccess
ORIGINAL
art_10.1186_1475-925X-9-24.pdf
art_10.1186_1475-925X-9-24.pdf
application/pdf
3107937
https://www.repo.uni-hannover.de/bitstream/123456789/572/1/art_10.1186_1475-925X-9-24.pdf
4fe9aa0b7fb9d35376f66fa9fefef243
MD5
1
TEXT
art_10.1186_1475-925X-9-24.pdf.txt
art_10.1186_1475-925X-9-24.pdf.txt
Extracted Text
text/plain
40615
https://www.repo.uni-hannover.de/bitstream/123456789/572/2/art_10.1186_1475-925X-9-24.pdf.txt
948aa904cfa63aa5e4e671b433680a83
MD5
2
THUMBNAIL
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art_10.1186_1475-925X-9-24.pdf.jpg
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image/jpeg
1611
https://www.repo.uni-hannover.de/bitstream/123456789/572/3/art_10.1186_1475-925X-9-24.pdf.jpg
ddaa2daec152016db76185ad4e7a889d
MD5
3
123456789/572
oai:www.repo.uni-hannover.de:123456789/572
2022-12-02 16:04:50.686
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/5822022-12-02T16:10:15Zcom_123456789_1col_123456789_8doc-type:Articleddc:540doc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Ude, Christian
9b2b6658-0e5b-459d-8774-4d959444f9ef
600
Schmidt-Hager, Jörg
a3c98ae6-0e34-423a-a9b9-e49828ed3452
600
Findeis, Michael
4b45016e-9651-4621-93bf-7a08c1cc61a2
600
John, Gernot Thomas
494501e3-a8cc-4874-a2df-0544c16987f0
600
Scheper, Thomas
05a38e4d-93b1-43ab-8ffc-43e89bb8a642
600
Beutel, Sascha
b0caeb51-6d08-4e37-84ac-0f06fcee5ad6
600
2016-10-28T09:54:26Z
2016-10-28T09:54:26Z
2014
Ude, Christian; Schmidt-Hager, Jörg; Findeis, M.; John, G.T.; Scheper, Thomas et al.: Application of an online-biomass sensor in an optical multisensory platform prototype for growth monitoring of biotechnical relevant microorganism and cell lines in single-use shake flasks. In: Sensors (Basel, Switzerland) 14 (2014), Nr. 9, S. 17390-17405. DOI: http://dx.doi.org/10.3390/s140917390
http://www.repo.uni-hannover.de/handle/123456789/582
http://dx.doi.org/10.15488/558
In the context of this work we evaluated a multisensory, noninvasive prototype platform for shake flask cultivations by monitoring three basic parameters (pH, pO2 and biomass). The focus lies on the evaluation of the biomass sensor based on backward light scattering. The application spectrum was expanded to four new organisms in addition to E. coli K12 and S. cerevisiae [1]. It could be shown that the sensor is appropriate for a wide range of standard microorganisms, e.g., L. zeae, K. pastoris, A. niger and CHO-K1. The biomass sensor signal could successfully be correlated and calibrated with well-known measurement methods like OD600, cell dry weight (CDW) and cell concentration. Logarithmic and Bleasdale-Nelder derived functions were adequate for data fitting. Measurements at low cell concentrations proved to be critical in terms of a high signal to noise ratio, but the integration of a custom made light shade in the shake flask improved these measurements significantly. This sensor based measurement method has a high potential to initiate a new generation of online bioprocess monitoring. Metabolic studies will particularly benefit from the multisensory data acquisition. The sensor is already used in labscale experiments for shake flask cultivations.
Made available in DSpace on 2016-10-28T09:54:26Z (GMT). No. of bitstreams: 0
Previous issue date: 2014
BMWi/AiF project
publishedVersion
eng
Basel : MDPI AG
Sensors 14 (2014), Nr. 9
1424-8220
http://dx.doi.org/10.3390/s140917390
CC BY 3.0 Unported
https://creativecommons.org/licenses/by/3.0/
animal
biochemical oxygen demand
cell count
cell proliferation
cell size
CHO cell line
Cricetulus
densitometry
device failure analysis
devices
equipment design
online system
photometry
physiology
refractometry
system analysis
Animals
Biological Oxygen Demand Analysis
Cell Count
Cell Proliferation
Cell Size
CHO Cells
Cricetulus
Densitometry
Equipment Design
Equipment Failure Analysis
Online Systems
Photometry
Refractometry
Systems Integration
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
540
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Application of an online-biomass sensor in an optical multisensory platform prototype for growth monitoring of biotechnical relevant microorganism and cell lines in single-use shake flasks
Article
Text
9
14
17390
17405
openAccess
ORIGINAL
sensors-14-17390-v2.pdf
sensors-14-17390-v2.pdf
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701899
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MD5
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123456789/582
oai:www.repo.uni-hannover.de:123456789/582
2022-12-02 17:10:15.007
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6022022-12-02T16:10:15Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Vanz, Ana Leticia
414d19cf-c205-4f73-8c5c-f4bdcf0c7a8d
600
Nimtz, Manfred
709ca5f1-c9e0-485a-8109-70be748d8c0c
600
Rinas, Ursula
7399be1d-6517-4855-8bdf-d054fa56ebaa
600
2016-10-31T07:59:01Z
2016-10-31T07:59:01Z
2014
Vanz, Ana Leticia.; Nimtz, M.; Rinas, Ursula: Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures. In: Microbial Cell Factories 13 (2014), Nr. 1, 23. DOI: http://dx.doi.org/10.1186/1475-2859-13-23
http://www.repo.uni-hannover.de/handle/123456789/602
http://dx.doi.org/10.15488/578
Background: Pichia pastoris is a popular yeast preferably employed for secretory protein production. Secretion is not always efficient and endoplasmic retention of proteins with aberrant folding properties, or when produced at exaggerated rates, can occur. In these cases production usually leads to an unfolded protein response (UPR) and the induction of the endoplasmic reticulum associated degradation (ERAD). P. pastoris is nowadays also an established host for secretory insulin precursor (IP) production, though little is known about the impact of IP production on the host cell physiology, in particular under industrially relevant production conditions. Here, we evaluate the cellular response to aox1 promoter-controlled, secretory IP production in controlled fed-batch processes using a proteome profiling approach.Results: Cells were first grown in a batch procedure using a defined medium with a high glycerol concentration. After glycerol depletion IP production was initiated by methanol addition which was kept constant through continuous methanol feeding. The most prominent changes of the intracellular proteome after the onset of methanol feeding were related to the enzymes of central carbon metabolism. In particular, the enzymes of the methanol dissimilatory pathway - virtually absent in the glycerol batch phase - dominated the proteome during the methanol fed-batch phase. Unexpectedly, a strong decrease of UPR and ERAD related proteins was also observed during methanol-induced IP production. Compared to non-producing control strains grown under identical conditions the UPR down-regulation was less pronounced indicating that IP production elicits a detectable but non prominent UPR response which is repressed by the general culture condition-dependent UPR down-regulation after the shift from glycerol to methanol.Conclusions: The passage of IP through the secretory pathway using an optimized IP vector and growing the strain at fed-batch conditions with a high initial glycerol concentration does not impose a significant burden on the secretory machinery even under conditions leading to an extracellular accumulation of ~ 3 g L-1 IP. The glycerol batch pre-induction culture conditions are associated with a high constitutive - recombinant protein production independent - induction of the UPR and ERAD pathways probably preconditioning the cells for effective IP secretion in the methanol fed-batch phase.
Made available in DSpace on 2016-10-31T07:59:01Z (GMT). No. of bitstreams: 0
Previous issue date: 2014
publishedVersion
eng
London : BioMed Central Ltd.
Microbial Cell Factories 13 (2014), Nr. 1
1475-2859
http://dx.doi.org/10.1186/1475-2859-13-23
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Endoplasmic reticulum associated degradation (ERAD)
Insulin precursor
Methanol metabolism
Pichia pastoris
Proteome
Two-dimensional gel electrophoresis
Unfolded protein response (UPR)
Batch Cell Culture Techniques
Endoplasmic Reticulum-Associated Degradation
Glycerol
Methanol
Pichia
Proinsulin
Proteome
Recombinant Proteins
Secretory Pathway
Unfolded Protein Response
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures
Article
Text
1
13
23
openAccess
ORIGINAL
art_10.1186_1475-2859-13-23.pdf
art_10.1186_1475-2859-13-23.pdf
application/pdf
1731945
https://www.repo.uni-hannover.de/bitstream/123456789/602/1/art_10.1186_1475-2859-13-23.pdf
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MD5
3
123456789/602
oai:www.repo.uni-hannover.de:123456789/602
2022-12-02 17:10:15.222
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6032022-12-02T16:10:14Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570
Khetarpal, Niyati
a665010d-1a3a-4ace-85cf-9653bcd2b450
600
Poddar, Ankur
d58d5516-e4ee-4c4f-a90a-088f37fc4bbd
600
Nemani, Satish K.
2f77d7ba-05e6-448c-9197-b5a2031c2ab5
600
Dhar, Nisha
742ae3d1-7e6a-4094-aec8-b6ac812cfcaf
600
Patil, Aravind
2c7e4305-e458-4ab4-867c-53cd0f85b62b
600
Negi, Priyanka
a2a56662-757d-4aed-9cdc-5c17f3b57d5c
600
Perween, Ashiya
c734db7c-0516-4a66-98a5-c273842ea2eb
600
Viswanathan, Ramaswamy
9ea27175-9bdb-4f8b-9af1-ea4de8fc4d70
600
Lünsdorf, Heinrich
a88e6531-1491-494f-a573-062cc3018a9b
600
Tyagi, Poornima
bbc5cb08-de96-4efa-bd28-1b35400a200c
600
Raut, Rajendra
2068afea-bfad-456c-9bb2-fedf03eb5f9a
600
Arora, Upsana
6008bec8-abf5-4deb-94ee-99e34d9682ac
600
Jain, Swatantra K.
bf779a40-7fb8-4a55-a9cb-ea7e3080acf1
600
Rinas, Ursula
7399be1d-6517-4855-8bdf-d054fa56ebaa
600
Swaminathan, Sathyamangalam
255197e1-c550-4b1a-9487-0dfde73a7572
600
Khanna, Navin
325ce125-a27a-4266-82e4-a94def981484
600
2016-10-31T07:59:01Z
2016-10-31T07:59:01Z
2013
Khetarpal N.; Poddar, A.; Nemani, Satish K.; Dhar, N.; Patil, A. et al.: Dengue-specific subviral nanoparticles: Design, creation and characterization. In: Journal of Nanobiotechnology 11 (2013), Nr. 1 , 15. DOI: http://dx.doi.org/10.1186/1477-3155-11-15
http://www.repo.uni-hannover.de/handle/123456789/603
http://dx.doi.org/10.15488/579
Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.
Made available in DSpace on 2016-10-31T07:59:01Z (GMT). No. of bitstreams: 0
Previous issue date: 2013
Council of Scientific & Industrial Research, Government of India
Department of Biotechnology, Government of India
publishedVersion
eng
London : BioMed Central Ltd.
Journal of Nanobiotechnology 11 (2013), Nr. 1
1477-3155
http://dx.doi.org/10.1186/1477-3155-11-15
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Bionanoparticles
Dengue envelope domain III
Hepatitis B surface antigen
Pichia pastoris
Virus-like particle
Bionanoparticles
Envelope domains
Hepatitis B surface antigen
Pichia Pastoris
Virus-like particles
Antigens
Diagnosis
Medical applications
Viruses
antigen
hepatitis B surface antigen
nanoparticle
s antigen
unclassified drug
virus envelope protein
cell extract
hybrid protein
nanoparticle
S envelope protein, hepatitis B virus
virus antigen
virus protein
article
cell lysate
Dengue virus
gene dosage
gene expression
molecular cloning
nonhuman
Pichia pastoris
protein domain
virus like agent
animal
chemistry
Chlorocebus aethiops
dengue
Dengue virus
isolation and purification
metabolism
physiology
Pichia
protein tertiary structure
species difference
ultrastructure
Vero cell line
virion
virology
Dengue virus
Hepatitis B virus
Pichia pastoris
Animals
Antigens, Viral
Cell Extracts
Cercopithecus aethiops
Dengue
Dengue Virus
Nanoparticles
Pichia
Protein Structure, Tertiary
Recombinant Fusion Proteins
Species Specificity
Vero Cells
Viral Envelope Proteins
Viral Proteins
Virion
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Dengue-specific subviral nanoparticles: Design, creation and characterization
Article
Text
1
11
15
openAccess
ORIGINAL
art_10.1186_1477-3155-11-15.pdf
art_10.1186_1477-3155-11-15.pdf
application/pdf
721316
https://www.repo.uni-hannover.de/bitstream/123456789/603/1/art_10.1186_1477-3155-11-15.pdf
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MD5
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41733
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MD5
3
123456789/603
oai:www.repo.uni-hannover.de:123456789/603
2022-12-02 17:10:14.999
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6072022-12-02T16:10:15Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Schmidt, Simone
d7984148-65d2-402a-b376-ef6ddf6c19ba
600
Stahl, Frank
9e2ff2ed-280d-46d6-a902-35bd40adfecd
600
Mutz, Kai-Oliver
382a8adf-6804-4961-abb8-027283d3ca38
600
Scheper, Thomas
05a38e4d-93b1-43ab-8ffc-43e89bb8a642
600
Hahn, Andreas
83433526-0762-4a50-8440-488dcf43c9cc
600
Schuchardt, Jan Philipp
17ca4573-3c79-435c-9144-a8559917c3c0
600
2016-10-31T10:06:38Z
2016-10-31T10:06:38Z
2012
Schmidt, Simone; Stahl, Frank; Mutz, Kai-Oliver; Scheper, Thomas; Hahn, Andreas et al.: Different gene expression profiles in normo- and dyslipidemic men after fish oil supplementation: Results from a randomized controlled trial. In: Lipids in Health and Disease 11 (2012), 105. DOI: http://dx.doi.org/10.1186/1476-511X-11-105
http://www.repo.uni-hannover.de/handle/123456789/607
http://dx.doi.org/10.15488/583
Background: Epidemiological studies have suggested the benefits of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on cardiovascular health, but only limited data are available describing n-3 PUFA regulated pathways in humans. The aim of this study was to investigate the effects of n-3 PUFA administration on whole genome expression profiles in the blood of normo- and dyslipidemic subjects. Methods. Differentially expressed genes were detected after four hours, one week and twelve weeks of supplementation with either fish oil (FO) or corn oil in normo- and dyslipidemic men using whole genome microarrays. Results: Independent of the oil, a significantly higher number of genes was regulated in dyslipidemic subjects compared to normolipidemic subjects. Pathway analyses discovered metabolisms dominantly affected by FO after twelve weeks of supplementation, including the lipid metabolism, immune system and cardiovascular diseases. Several pro-inflammatory genes, in particular, were down-regulated in dyslipidemic subjects, indicating the immune-modulatory and anti-inflammatory capability of FO and its bioactive FAs, eicosapentaenoic acid and docosahexaenoic acid. Conclusions: This is the first study showing significant differences in gene expression profiles between normo- and dyslipidemic men after FO supplementation. Further studies need to clarify the exact role of n-3 PUFAs in pathways and metabolisms which were identified as being regulated after FO supplementation in this study. Trial registration. ClinicalTrials.gov (ID: NCT01089231).
Made available in DSpace on 2016-10-31T10:06:38Z (GMT). No. of bitstreams: 0
Previous issue date: 2012
BMBF
publishedVersion
eng
London : BioMed Central Ltd.
Lipids in Health and Disease 11 (2012)
1476-511X
http://dx.doi.org/10.1186/1476-511X-11-105
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Cardiovascular disease
Dyslipidemia
Gene regulation
Genome microarrays
Hypertriglyceridemia
Lipid metabolism
Omega-3 fatty acids
Omega-3 index
Pathway analysis
cholesterol
docosahexaenoic acid
fish oil
high density lipoprotein
icosapentaenoic acid
low density lipoprotein
membrane lipid
omega 3 fatty acid
triacylglycerol
adult
antiinflammatory activity
article
cardiovascular disease
clinical article
controlled study
diet supplementation
double blind procedure
down regulation
dyslipidemia
erythrocyte membrane
gene expression profiling
gene expression regulation
human
immune system
immunomodulation
lipid blood level
lipid composition
lipid metabolism
microarray analysis
nucleotide sequence
randomized controlled trial
Adult
Corn Oil
Double-Blind Method
Dyslipidemias
Erythrocyte Membrane
Fatty Acids
Fatty Acids, Omega-3
Fish Oils
Humans
Immune System
Lipid Metabolism
Lipids
Male
Middle Aged
Oligonucleotide Array Sequence Analysis
RNA
Transcriptome
Zea mays
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Different gene expression profiles in normo- and dyslipidemic men after fish oil supplementation: Results from a randomized controlled trial
Article
Text
11
105
openAccess
ORIGINAL
art_10.1186_1476-511X-11-105.pdf
art_10.1186_1476-511X-11-105.pdf
application/pdf
619197
https://www.repo.uni-hannover.de/bitstream/123456789/607/1/art_10.1186_1476-511X-11-105.pdf
582331339abd7331df89d83cd7aa6f15
MD5
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MD5
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123456789/607
oai:www.repo.uni-hannover.de:123456789/607
2022-12-02 17:10:15.386
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6082022-12-02T16:14:09Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Hass, Ralf
8dfe1a2a-e5d4-48c2-b7fc-97ef08dfedc7
600
Kasper, Cornelia
8b273750-8fa6-4393-9c1e-b3d7ff3bddee
600
Böhm, Stefanie
a1df014b-834d-4119-968f-c77c53b3595d
600
Jacobs, Roland
145d77fa-1144-4ff9-956b-2eb356e7017e
600
2016-10-31T10:06:39Z
2016-10-31T10:06:39Z
2011
Hass, R.; Kasper, Cornelia; Böhm, Stefanie; Jacobs, R.: Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC. In: Cell Communication and Signaling 9 (2011), 12. DOI: http://dx.doi.org/10.1186/1478-811X-9-12
http://www.repo.uni-hannover.de/handle/123456789/608
http://dx.doi.org/10.15488/584
The mesenchymal stroma harbors an important population of cells that possess stem cell-like characteristics including self renewal and differentiation capacities and can be derived from a variety of different sources. These multipotent mesenchymal stem cells (MSC) can be found in nearly all tissues and are mostly located in perivascular niches. MSC have migratory abilities and can secrete protective factors and act as a primary matrix for tissue regeneration during inflammation, tissue injuries and certain cancers. These functions underlie the important physiological roles of MSC and underscore a significant potential for the clinical use of distinct populations from the various tissues. MSC derived from different adult (adipose tissue, peripheral blood, bone marrow) and neonatal tissues (particular parts of the placenta and umbilical cord) are therefore compared in this mini-review with respect to their cell biological properties, surface marker expression and proliferative capacities. In addition, several MSC functions including in vitro and in vivo differentiation capacities within a variety of lineages and immune-modulatory properties are highlighted. Differences in the extracellular milieu such as the presence of interacting neighbouring cell populations, exposure to proteases or a hypoxic microenvironment contribute to functional developments within MSC populations originating from different tissues, and intracellular conditions such as the expression levels of certain micro RNAs can additionally balance MSC function and fate.
Made available in DSpace on 2016-10-31T10:06:39Z (GMT). No. of bitstreams: 0
Previous issue date: 2011
DFG/SFB738/A5
DFG/EXC/REBIRTH
publishedVersion
eng
London : BioMed Central Ltd.
Cell Communication and Signaling 9 (2011)
1478-811X
http://dx.doi.org/10.1186/1478-811X-9-12
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
gamma interferon
Jagged1
mycophenolic acid
rapamycin
toll like receptor 3
adipogenesis
age distribution
binding affinity
cell differentiation
cell function
cell hypoxia
cell interaction
cell lineage
cell migration
cell population
cellular immunity
density gradient centrifugation
graft versus host reaction
human
immunogenicity
immunomodulation
immunoregulation
in vitro study
in vivo study
low drug dose
mesenchymal stem cell
microenvironment
nonhuman
osteoblast
oxidative stress
placenta
plasticity
priority journal
protein expression
review
synapse
tissue distribution
umbilical cord
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC
Article
Text
9
12
openAccess
ORIGINAL
art_10.1186_1478-811X-9-12.pdf
art_10.1186_1478-811X-9-12.pdf
application/pdf
2159955
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MD5
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MD5
3
123456789/608
oai:www.repo.uni-hannover.de:123456789/608
2022-12-02 17:14:09.542
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6122022-12-02T16:11:40Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Schmidt, Simone
d7984148-65d2-402a-b376-ef6ddf6c19ba
600
Willers, Janina
dd7fdfae-5935-422e-82df-892ba07197f7
600
Riecker, Sabine
6c20156f-5b5f-4adf-8953-b5eb56cbafd0
600
Möller, Katharina
50199f17-a931-4a6e-b780-530843dcb04d
600
Schuchardt, Jan Philipp
17ca4573-3c79-435c-9144-a8559917c3c0
600
Hahn, Andreas
83433526-0762-4a50-8440-488dcf43c9cc
600
2016-10-31T10:06:39Z
2016-10-31T10:06:39Z
2015
Schmidt, Simone; Willers, Janina; Riecker, Sabine; Möller, Katharina; Schuchardt, Jan Philipp et al.: Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: An open-label intervention study. In: Lipids in Health and Disease 14 (2015), 4: DOI: http://dx.doi.org/10.1186/1476-511X-14-4
http://www.repo.uni-hannover.de/handle/123456789/612
http://dx.doi.org/10.15488/588
Background: Omega-3 polyunsaturated fatty acids (n-3 PUFAs) show beneficial effects on cardiovascular health and cognitive functions, but the underlying molecular mechanisms are not completely understood. Because of the fact that cytoskeleton dynamics affect almost every cellular process, the regulation of cytoskeletal dynamics could be a new pathway by which n-3 PUFAs exert their effects on cellular level. Methods: A 12-week open-label intervention study with 12 healthy men was conducted to determine the effects of 2.7 g/d n-3 PUFA on changes in mRNA expression of cytoskeleton-associated genes by quantitative real-time PCR in whole blood. Furthermore, the actin content in red blood cells was analyzed by immunofluorescence imaging. Results: N-3 PUFA supplementation resulted in a significant down-regulation of cytoskeleton-associated genes, in particular three GTPases (RAC1, RHOA, CDC42), three kinases (ROCK1, PAK2, LIMK), two Wiskott-Aldrich syndrome proteins (WASL, WASF2) as well as actin related protein 2/3 complex (ARPC2, ARPC3) and cofilin (CFL1). Variability in F-actin content between subjects was high; reduced actin content was only reduced within group evaluation. Conclusions: Reduced cytoskeleton-associated gene expression after n-3 PUFA supplementation suggests that regulation of cytoskeleton dynamics might be an additional way by which n-3 PUFAs exert their cellular effects. Concerning F-actin, this analysis did not reveal unmistakable results impeding a generalized conclusion.
Made available in DSpace on 2016-10-31T10:06:39Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
publishedVersion
eng
London : BioMed Central Ltd.
Lipids in Health and Disease 14 (2015)
1476-511X
http://dx.doi.org/10.1186/1476-511X-14-4
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
Cytoskeleton
F-actin
Gene-expression
GTPases
n-3 PUFA
Omega-3 index
Red blood cells
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: An open-label intervention study
Article
Text
14
4
openAccess
ORIGINAL
art_10.1186_1476-511X-14-4.pdf
art_10.1186_1476-511X-14-4.pdf
application/pdf
855640
https://www.repo.uni-hannover.de/bitstream/123456789/612/1/art_10.1186_1476-511X-14-4.pdf
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MD5
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TEXT
art_10.1186_1476-511X-14-4.pdf.txt
art_10.1186_1476-511X-14-4.pdf.txt
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text/plain
39101
https://www.repo.uni-hannover.de/bitstream/123456789/612/2/art_10.1186_1476-511X-14-4.pdf.txt
d9af955a222988e4e43d885f75f789ee
MD5
2
THUMBNAIL
art_10.1186_1476-511X-14-4.pdf.jpg
art_10.1186_1476-511X-14-4.pdf.jpg
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b0d233e926c39efbded7e49182109d40
MD5
3
123456789/612
oai:www.repo.uni-hannover.de:123456789/612
2022-12-02 17:11:40.98
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6172022-12-02T19:35:26Zcom_123456789_11col_123456789_12doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Niemiec, Emilia
a5261180-9e48-4ab7-acd6-ec519ad8ab82
-1
Howard, Heidi Carmen
04b7d87a-8645-4e15-b351-3fccb4cc8465
-1
2016-10-31T10:06:41Z
2016-10-31T10:06:41Z
2016
Niemiec, Emilia; Howard, Heidi Carmen: Ethical issues in consumer genome sequencing: Use of consumers' samples and data. In: Applied and Translational Genomics 8 (2016), S. 23-30. DOI: http://dx.doi.org/10.1016/j.atg.2016.01.005
http://www.repo.uni-hannover.de/handle/123456789/617
http://dx.doi.org/10.15488/593
High throughput approaches such as whole genome sequencing (WGS) and whole exome sequencing (WES) create an unprecedented amount of data providing powerful resources for clinical care and research. Recently, WGS and WES services have been made available by commercial direct-to-consumer (DTC) companies. The DTC offer of genetic testing (GT) has already brought attention to potentially problematic issues such as the adequacy of consumers' informed consent and transparency of companies' research activities. In this study, we analysed the websites of four DTC GT companies offering WGS and/or WES with regard to their policies governing storage and future use of consumers' data and samples. The results are discussed in relation to recommendations and guiding principles such as the "Statement of the European Society of Human Genetics on DTC GT for health-related purposes" (2010) and the "Framework for responsible sharing of genomic and health-related data" (Global Alliance for Genomics and Health, 2014). The analysis reveals that some companies may store and use consumers' samples or sequencing data for unspecified research and share the data with third parties. Moreover, the companies do not provide sufficient or clear information to consumers about this, which can undermine the validity of the consent process. Furthermore, while all companies state that they provide privacy safeguards for data and mention the limitations of these, information about the possibility of re-identification is lacking. Finally, although the companies that may conduct research do include information regarding proprietary claims and commercialisation of the results, it is not clear whether consumers are aware of the consequences of these policies. These results indicate that DTC GT companies still need to improve the transparency regarding handling of consumers' samples and data, including having an explicit and clear consent process for research activities.
Made available in DSpace on 2016-10-31T10:06:41Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
Swedish Foundation for Humanities and Social Sciences/M13-0260:1
Biobanking and Molecular Resource Infrastructure of Sweden (BBMRI.se)
BMRI-ERIC
CHIP ME COST Action IS130
publishedVersion
eng
Amsterdam : Elsevier
Applied and Translational Genomics 8 (2016)
2212-0661
http://dx.doi.org/10.1016/j.atg.2016.01.005
CC BY-NC-ND 4.0 Unported
https://creativecommons.org/licenses/by-nc-nd/4.0/
Consent
Consumer genomics
Direct-to-consumer genetic testing
Human genome research
Whole-exome sequencing
Whole-genome sequencing
access to information
Article
consumer health information
data processing
gene sequence
genetic database
health care policy
human
information dissemination
information processing
informed consent
medical research
practice guideline
priority journal
sequence analysis
web browser
whole exome sequencing
whole genome sequencing
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Ethical issues in consumer genome sequencing: Use of consumers' samples and data
Article
Text
8
23
30
openAccess
ORIGINAL
1-s2.0-S2212066116300059-main.pdf
1-s2.0-S2212066116300059-main.pdf
application/pdf
246450
https://www.repo.uni-hannover.de/bitstream/123456789/617/1/1-s2.0-S2212066116300059-main.pdf
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MD5
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1-s2.0-S2212066116300059-main.pdf.txt
1-s2.0-S2212066116300059-main.pdf.txt
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57796
https://www.repo.uni-hannover.de/bitstream/123456789/617/2/1-s2.0-S2212066116300059-main.pdf.txt
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MD5
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THUMBNAIL
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69bfd3120b19a6a96f17fa4c7b12faa5
MD5
3
123456789/617
oai:www.repo.uni-hannover.de:123456789/617
2022-12-02 20:35:26.331
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6192022-12-02T15:04:50Zcom_123456789_1col_123456789_6doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570ddc:600
Erdmann, Nina
af82ca99-1b8b-4f08-aea0-2c6c4d9a21e4
600
Bondarenko, Alexandr
37a97edc-1733-47b4-984a-caa085c2ce36
600
Hewicker-Trautwein, Marion
5fb7cd49-b3ff-4d59-94af-37cd7f225dc4
600
Angrisani, Nina
ccea4e82-63f4-449e-b0c1-9b8b032891ee
600
Reifenrath, Janin
4cd37190-194b-46f4-a59f-f6121ea7e075
600
Lucas, Arne
be834275-c1c0-41ef-bb94-8d99789dadf4
600
Meyer-Lindenberg, Andrea
d7c7122b-a8f1-497a-b575-a7bf155f7a59
600
2016-10-31T13:37:18Z
2016-10-31T13:37:18Z
2010
Erdmann, N.; Bondarenko, A.; Hewicker-Trautwein, M.; Angrisani, N.; Reifenrath, J.et al.: Evaluation of the soft tissue biocompatibility of MgCa0.8 and surgical steel 316L in vivo: A comparative study in rabbits. In: BioMedical Engineering Online 9 (2010), 63. DOI: http://dx.doi.org/10.1186/1475-925X-9-63
http://www.repo.uni-hannover.de/handle/123456789/619
http://dx.doi.org/10.15488/595
Background: Recent studies have shown the potential suitability of magnesium alloys as biodegradable implants. The aim of the present study was to compare the soft tissue biocompatibility of MgCa0.8 and commonly used surgical steel in vivo.Methods: A biodegradable magnesium calcium alloy (MgCa0.8) and surgical steel (S316L), as a control, were investigated. Screws of identical geometrical conformation were implanted into the tibiae of 40 rabbits for a postoperative follow up of two, four, six and eight weeks. The tibialis cranialis muscle was in direct vicinity of the screw head and thus embedded in paraffin and histologically and immunohistochemically assessed. Haematoxylin and eosin staining was performed to identify macrophages, giant cells and heterophil granulocytes as well as the extent of tissue fibrosis and necrosis. Mouse anti-CD79α and rat anti-CD3 monoclonal primary antibodies were used for B- and T-lymphocyte detection. Evaluation of all sections was performed by applying a semi-quantitative score.Results: Clinically, both implant materials were tolerated well. Histology revealed that a layer of fibrous tissue had formed between implant and overlying muscle in MgCa0.8 and S316L, which was demarcated by a layer of synoviocyte-like cells at its interface to the implant. In MgCa0.8 implants cavities were detected within the fibrous tissue, which were surrounded by the same kind of cell type. The thickness of the fibrous layer and the amount of tissue necrosis and cellular infiltrations gradually decreased in S316L. In contrast, a decrease could only be noted in the first weeks of implantation in MgCa0.8, whereas parameters were increasing again at the end of the observation period. B-lymphocytes were found more often in MgCa0.8 indicating humoral immunity and the presence of soluble antigens. Conversely, S316L displayed a higher quantity of T-lymphocytes.Conclusions: Moderate inflammation was detected in both implant materials and resolved to a minimum during the first weeks indicating comparable biocompatibility for MgCa0.8 and S316L. Thus, the application of MgCa0.8 as biodegradable implant material seems conceivable. Since the inflammatory parameters were re-increasing at the end of the observation period in MgCa0.8 it is important to observe the development of inflammation over a longer time period in addition to the present study.
Made available in DSpace on 2016-10-31T13:37:18Z (GMT). No. of bitstreams: 0
Previous issue date: 2010
DFG/Collaborative Research Centre/599
publishedVersion
eng
London : BioMed Central Ltd.
BioMedical Engineering Online 9 (2010)
1475-925X
http://dx.doi.org/10.1186/1475-925X-9-63
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Anti-cd3
B lymphocytes
Cell types
Cellular infiltration
Commonly used
Comparative studies
Fibrous tissue
Follow up
Geometrical conformation
Giant cells
Haematoxylin
Humoral immunity
Implant materials
In-vivo
Magnesium-calcium alloys
Observation Period
Screw head
Semi-quantitative
Soft tissue
Synoviocytes
Time-periods
Tissue necrosis
Antibodies
Calcium alloys
Chemical detection
Magnesium
Magnesium alloys
Muscle
Paraffin waxes
Paraffins
Pathology
Screws
Surgery
Biocompatibility
Oryctolagus cuniculus
Rattus
alloy
biomaterial
calcium
magnesium
steel
animal
article
biodegradable implant
bone screw
chemically induced disorder
chemistry
comparative study
drug effect
female
general surgery
inflammation
materials testing
methodology
pathology
rabbit
radiography
skeletal muscle
tibia
Absorbable Implants
Alloys
Animals
Biocompatible Materials
Bone Screws
Calcium
Female
General Surgery
Inflammation
Magnesium
Materials Testing
Muscle, Skeletal
Rabbits
Steel
Tibia
Dewey Decimal Classification::600 | Technik
600
600
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Evaluation of the soft tissue biocompatibility of MgCa0.8 and surgical steel 316L in vivo: A comparative study in rabbits
Article
Text
9
63
openAccess
ORIGINAL
art_10.1186_1475-925X-9-63.pdf
art_10.1186_1475-925X-9-63.pdf
application/pdf
4522440
https://www.repo.uni-hannover.de/bitstream/123456789/619/1/art_10.1186_1475-925X-9-63.pdf
9f41a2e6d65107ae33a681e34b78e78e
MD5
1
TEXT
art_10.1186_1475-925X-9-63.pdf.txt
art_10.1186_1475-925X-9-63.pdf.txt
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text/plain
51450
https://www.repo.uni-hannover.de/bitstream/123456789/619/2/art_10.1186_1475-925X-9-63.pdf.txt
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MD5
2
THUMBNAIL
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MD5
3
123456789/619
oai:www.repo.uni-hannover.de:123456789/619
2022-12-02 16:04:50.359
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6202022-12-02T15:19:58Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Terefe-Ayana, Diro
8c9652b6-ea15-42ff-ba63-79e808ed81ac
600
Kaufmann, Helgard
0fd8b70a-6784-4ba0-98ba-794951288095
600
Linde, Marcus
7c1ceb3e-3dbe-4e91-9fc1-a0a7159b9e37
600
Debener, Thomas
2aedee57-8d8b-49ca-9c67-1a988b8fba75
600
2016-10-31T13:37:19Z
2016-10-31T13:37:19Z
2012
Terefe-Ayana, Diro; Kaufmann, Helgard; Linde, Marcus; Debener, Thomas: Evolution of the Rdr1 TNL-cluster in roses and other Rosaceous species. In: BMC Genomics 13 (2012), Nr. 1, 409. DOI: http://dx.doi.org/10.1186/1471-2164-13-409
http://www.repo.uni-hannover.de/handle/123456789/620
http://dx.doi.org/10.15488/596
Background: The resistance of plants to pathogens relies on two lines of defense: a basal defense response and a pathogen-specific system, in which resistance (R) genes induce defense reactions after detection of pathogen-associated molecular patterns (PAMPS). In the specific system, a so-called arms race has developed in which the emergence of new races of a pathogen leads to the diversification of plant resistance genes to counteract the pathogens' effect. The mechanism of resistance gene diversification has been elucidated well for short-lived annual species, but data are mostly lacking for long-lived perennial and clonally propagated plants, such as roses. We analyzed the rose black spot resistance gene, Rdr1, in five members of the Rosaceae: Rosa multiflora, Rosa rugosa, Fragaria vesca (strawberry), Malus x domestica (apple) and Prunus persica (peach), and we present the deduced possible mechanism of R-gene diversification.Results: We sequenced a 340.4-kb region from R. rugosa orthologous to the Rdr1 locus in R. multiflora. Apart from some deletions and rearrangements, the two loci display a high degree of synteny. Additionally, less pronounced synteny is found with an orthologous locus in strawberry but is absent in peach and apple, where genes from the Rdr1 locus are distributed on two different chromosomes. An analysis of 20 TIR-NBS-LRR (TNL) genes obtained from R. rugosa and R. multiflora revealed illegitimate recombination, gene conversion, unequal crossing over, indels, point mutations and transposable elements as mechanisms of diversification.A phylogenetic analysis of 53 complete TNL genes from the five Rosaceae species revealed that with the exception of some genes from apple and peach, most of the genes occur in species-specific clusters, indicating that recent TNL gene diversification began prior to the split of Rosa from Fragaria in the Rosoideae and peach from apple in the Spiraeoideae and continued after the split in individual species. Sequence similarity of up to 99% is obtained between two R. multiflora TNL paralogs, indicating a very recent duplication.Conclusions: The mechanisms by which TNL genes from perennial Rosaceae diversify are mainly similar to those from annual plant species. However, most TNL genes appear to be of recent origin, likely due to recent duplications, supporting the hypothesis that TNL genes in woody perennials are generally younger than those from annuals. This recent origin might facilitate the development of new resistance specificities, compensating for longer generation times in woody perennials.
Made available in DSpace on 2016-10-31T13:37:19Z (GMT). No. of bitstreams: 0
Previous issue date: 2012
DFG/DE 511/4-1
DFG/DE 511/4-2
publishedVersion
eng
London : BioMed Central Ltd.
BMC Genomics 13 (2012), Nr. 1
1471-2164
http://dx.doi.org/10.1186/1471-2164-13-409
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
apple
article
chromosome analysis
controlled study
Fragaria
Fragaria vesca
gene cluster
gene conversion
gene deletion
gene duplication
gene locus
gene rearrangement
gene sequence
genetic recombination
genetic similarity
genetic variability
nonhuman
nucleotide sequence
peach
phylogeny
plant gene
point mutation
promoter region
R gene
Rdr1 gene
Rosa multiflora
Rosa rugosa
Rosaceae
rose
Rosoideae
sequence alignment
species difference
Spiraeoideae
strawberry
TNL gene
transposon
Chromosomes
Cluster Analysis
Contig Mapping
Evolution, Molecular
Fragaria
Genes, Plant
Genetic Loci
Malus
Phylogeny
Plant Proteins
Prunus
Repressor Proteins
Rosa
Fragaria
Fragaria vesca
Fragaria x ananassa
Malus x domestica
Prunus persica
Rosa
Rosa multiflora
Rosa rugosa
Rosaceae
Rosoideae
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Evolution of the Rdr1 TNL-cluster in roses and other Rosaceous species
Article
Text
1
13
409
openAccess
ORIGINAL
art_10.1186_1471-2164-13-409.pdf
art_10.1186_1471-2164-13-409.pdf
application/pdf
2272639
https://www.repo.uni-hannover.de/bitstream/123456789/620/1/art_10.1186_1471-2164-13-409.pdf
31651e463d139b4b00c5ba3465750acf
MD5
1
TEXT
art_10.1186_1471-2164-13-409.pdf.txt
art_10.1186_1471-2164-13-409.pdf.txt
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text/plain
66718
https://www.repo.uni-hannover.de/bitstream/123456789/620/2/art_10.1186_1471-2164-13-409.pdf.txt
682436ccf1aece8a4f24ccd4c38b8e9b
MD5
2
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f398579fefec86a364d45945ae6c7da0
MD5
3
123456789/620
oai:www.repo.uni-hannover.de:123456789/620
2022-12-02 16:19:58.761
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6272022-12-02T15:19:58Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Majore, Ingrida
9e430843-d635-43d4-9467-aceb6e80db8a
600
Moretti, Pierre
5ad97ca9-be83-47f6-8eda-aafe858fd885
600
Hass, Ralf
8dfe1a2a-e5d4-48c2-b7fc-97ef08dfedc7
600
Kasper, Cornelia
8b273750-8fa6-4393-9c1e-b3d7ff3bddee
600
2016-10-31T13:37:22Z
2016-10-31T13:37:22Z
2009
Majore, Ingrida; Moretti, Pierre; Hass, R.; Kasper, Cornelia: Identification of subpopulations in mesenchymal stem cell-like cultures from human umbilical cord. In: Cell Communication and Signaling 7 (2009), 6. DOI: http://dx.doi.org/10.1186/1478-811X-7-6
http://www.repo.uni-hannover.de/handle/123456789/627
http://dx.doi.org/10.15488/603
Background: A variety of cell types can be identified in the adherent fraction of bone marrow mononuclear cells including more primitive and embryonic-like stem cells, mesenchymal stem cells (MSC), lineage-committed progenitors as well as mature cells such as osteoblasts and fibroblasts. Different methods are described for the isolation of single bone marrow stem cell subpopulations - beginning from ordinary size sieving, long term cultivation under specific conditions to FACS-based approaches. Besides bone marrow-derived subpopulations, also other tissues including human umbilical cord (UC) have been recently suggested to provide a potential source for MSC. Although of clinical importance, these UC-derived MSC populations remain to be characterized. It was thus the aim of the present study to identify possible subpopulations in cultures of MSC-like cells obtained from UC. We used counterflow centrifugal elutriation (CCE) as a novel strategy to successfully address this question. Results: UC-derived primary cells were separated by CCE and revealed differentially-sized populations in the fractions. Thus, a subpopulation with an average diameter of about 11 μm and a small flat cell body was compared to a large sized subpopulation of about 19 μm average diameter. Flow cytometric analysis revealed the expression of certain MSC stem cell markers including CD44, CD73, CD90 and CD105, respectively, although these markers were expressed at higher levels in the small-sized population. Moreover, this small-sized subpopulation exhibited a higher proliferative capacity as compared to the total UC-derived primary cultures and the large-sized cells and demonstrated a reduced amount of aging cells. Conclusion: Using the CCE technique, we were the first to demonstrate a subpopulation of small-sized UC-derived primary cells carrying MSC-like characteristics according to the presence of various mesenchymal stem cell markers. This is also supported by the high proliferative capacity of these MSC-like cells as compared to whole primary culture or other UC-derived subpopulations. The accumulation of a self-renewing MSC-like subpopulation by CCE with low expression levels of the aging marker senescence-associated β-galactosidase provides a valuable tool in the regenerative medicine and an alternative to bone-marrow-derived MSC.
Made available in DSpace on 2016-10-31T13:37:22Z (GMT). No. of bitstreams: 0
Previous issue date: 2009
DFG/KA 1784/5-1
publishedVersion
eng
London : BioMed Central Ltd.
Cell Communication and Signaling 7 (2009)
1478-811X
http://dx.doi.org/10.1186/1478-811X-7-6
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Counterflow Centrifugal Elutriation
CCE
mesenchymal stem cells
MSC
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Identification of subpopulations in mesenchymal stem cell-like cultures from human umbilical cord
Article
Text
7
6
openAccess
ORIGINAL
art_10.1186_1478-811X-7-6.pdf
art_10.1186_1478-811X-7-6.pdf
application/pdf
1622568
https://www.repo.uni-hannover.de/bitstream/123456789/627/1/art_10.1186_1478-811X-7-6.pdf
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MD5
1
TEXT
art_10.1186_1478-811X-7-6.pdf.txt
art_10.1186_1478-811X-7-6.pdf.txt
Extracted Text
text/plain
33310
https://www.repo.uni-hannover.de/bitstream/123456789/627/2/art_10.1186_1478-811X-7-6.pdf.txt
c4f772f1db28c238942bf1784b827d50
MD5
2
THUMBNAIL
art_10.1186_1478-811X-7-6.pdf.jpg
art_10.1186_1478-811X-7-6.pdf.jpg
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image/jpeg
1755
https://www.repo.uni-hannover.de/bitstream/123456789/627/3/art_10.1186_1478-811X-7-6.pdf.jpg
79285bccaeea6ec30451447886c179c2
MD5
3
123456789/627
oai:www.repo.uni-hannover.de:123456789/627
2022-12-02 16:19:58.749
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6292022-12-02T15:04:50Zcom_123456789_1col_123456789_6doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570ddc:600
Waizy, Hazibullah
9c572edb-e79c-423b-bf7b-7eae0fb4be7f
600
Weizbauer, Andreas
e48e0720-8bfb-4054-9d9f-fadd951986de
600
Modrejewski, Christian
90572c1a-b0e3-43ba-b96d-c8eefedacc97
600
Witte, Frank
2ab1d256-cd49-4702-a910-856baf7d0762
600
Windhagen, Henning
e8cd937e-80bc-4776-b2a2-3d4225f9943c
600
Lucas, Arne
be834275-c1c0-41ef-bb94-8d99789dadf4
600
Kieke, Marc D.K.
6323b97b-0bc8-4c60-9271-cef8acb19cff
600
Denkena, Berend
9acacde7-c7cf-4f5a-87fd-2018e746a754
600
Behrens, Peter
83509335-eef2-4ca9-a566-48da2c50ed01
600
Meyer-Lindenberg, Andrea
d7c7122b-a8f1-497a-b575-a7bf155f7a59
600
Bach, Friedrich-Wilhelm
1da5ac04-aaf6-492a-a5a3-7bf010bbee30
600
Thorey, Fritz
28421f98-2ab8-4735-b545-d06282a2cce5
600
2016-10-31T13:38:59Z
2016-10-31T13:38:59Z
2012
Waizy, H.; Weizbauer, A.; Modrejewski, C.; Witte, F.; Windhagen, H. et al.: In vitro corrosion of ZEK100 plates in Hank's Balanced Salt Solution. In: BioMedical Engineering Online 11 (2012), 12. DOI: http://dx.doi.org/10.1186/1475-925X-11-12
http://www.repo.uni-hannover.de/handle/123456789/629
http://dx.doi.org/10.15488/605
Background: In recent years magnesium alloys have been intensively investigated as potential resorbable materials with appropriate mechanical and corrosion properties. Particularly in orthopedic research magnesium is interesting because of its mechanical properties close to those of natural bone, the prevention of both stress shielding and removal of the implant after surgery.Methods: ZEK100 plates were examined in this in vitro study with Hank's Balanced Salt Solution under physiological conditions with a constant laminar flow rate. After 14, 28 and 42 days of immersion the ZEK100 plates were mechanically tested via four point bending test. The surfaces of the immersed specimens were characterized by SEM, EDX and XRD.Results: The four point bending test displayed an increased bending strength after 6 weeks immersion compared to the 2 week group and 4 week group. The characterization of the surface revealed the presence of high amounts of O, P and Ca on the surface and small Mg content. This indicates the precipitation of calcium phosphates with low solubility on the surface of the ZEK100 plates.Conclusions: The results of the present in vitro study indicate that ZEK100 is a potential candidate for degradable orthopedic implants. Further investigations are needed to examine the degradation behavior.
Made available in DSpace on 2016-10-31T13:38:59Z (GMT). No. of bitstreams: 0
Previous issue date: 2012
DFG/SFB/599
DFG/SFB/599
publishedVersion
eng
London : BioMed Central Ltd.
BioMedical Engineering Online 11 (2012)
1475-925X
http://dx.doi.org/10.1186/1475-925X-11-12
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Corrosion
In vitro study
Magnesium alloy
Plates
Corrosion property
Degradation behavior
Four-point bending test
Hank's balanced salt solutions
In-vitro
Mg content
Natural bone
Orthopedic implant
Physiological condition
Plates
Resorbable materials
Stress shielding
Calcium phosphate
Corrosion
Magnesium
Magnesium alloys
Mechanical properties
Salt removal
Surfaces
Magnesium printing plates
alloy
bicarbonate
biomaterial
Hanks Balanced Salt Solution
isotonic solution
magnesium
article
chemistry
corrosion
hydrodynamics
immersion
mechanics
surface property
Alloys
Bicarbonates
Biocompatible Materials
Corrosion
Hydrodynamics
Immersion
Isotonic Solutions
Magnesium
Mechanical Processes
Surface Properties
Dewey Decimal Classification::600 | Technik
600
600
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
In vitro corrosion of ZEK100 plates in Hank's Balanced Salt Solution
Article
Text
11
12
openAccess
ORIGINAL
art_10.1186_1475-925X-11-12.pdf
art_10.1186_1475-925X-11-12.pdf
application/pdf
2841348
https://www.repo.uni-hannover.de/bitstream/123456789/629/1/art_10.1186_1475-925X-11-12.pdf
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MD5
1
TEXT
art_10.1186_1475-925X-11-12.pdf.txt
art_10.1186_1475-925X-11-12.pdf.txt
Extracted Text
text/plain
34377
https://www.repo.uni-hannover.de/bitstream/123456789/629/2/art_10.1186_1475-925X-11-12.pdf.txt
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MD5
2
THUMBNAIL
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art_10.1186_1475-925X-11-12.pdf.jpg
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image/jpeg
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https://www.repo.uni-hannover.de/bitstream/123456789/629/3/art_10.1186_1475-925X-11-12.pdf.jpg
c16f60eaff3c1af96454ea2825b5925d
MD5
3
123456789/629
oai:www.repo.uni-hannover.de:123456789/629
2022-12-02 16:04:50.671
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6322022-12-02T15:19:58Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Schuchardt, Jan Philipp
17ca4573-3c79-435c-9144-a8559917c3c0
600
Schneider, Inga
eb229b6f-9351-4cc6-8341-b8ab597d3464
600
Meyer, Henrike
15fe35dc-afae-422f-ae46-9bcfd7b43648
600
Neubronner, Juliane
3a8f346b-101c-42ab-aecd-264878055e41
600
Schacky, C. von
5587f22f-1efa-4579-8cff-eb6515121d0f
600
Hahn, Andreas
83433526-0762-4a50-8440-488dcf43c9cc
600
2016-11-02T08:33:43Z
2016-11-02T08:33:43Z
2011
Schuchardt, Jan Philipp; Schneider, Inga; Meyer, Henrike; Neubronner, Juliane; Von Schacky, C. et al.: Incorporation of EPA and DHA into plasma phospholipids in response to different omega-3 fatty acid formulations - A comparative bioavailability study of fish oil vs. krill oil. In: Lipids in Health and Disease 10 (2011), 145. DOI: http://dx.doi.org/10.1186/1476-511X-10-145
http://www.repo.uni-hannover.de/handle/123456789/632
http://dx.doi.org/10.15488/608
Background: Bioavailability of omega-3 fatty acids (FA) depends on their chemical form. Superior bioavailability has been suggested for phospholipid (PL) bound omega-3 FA in krill oil, but identical doses of different chemical forms have not been compared. Methods. In a double-blinded crossover trial, we compared the uptake of three EPA+DHA formulations derived from fish oil (re-esterified triacylglycerides [rTAG], ethyl-esters [EE]) and krill oil (mainly PL). Changes of the FA compositions in plasma PL were used as a proxy for bioavailability. Twelve healthy young men (mean age 31 y) were randomized to 1680 mg EPA+DHA given either as rTAG, EE or krill oil. FA levels in plasma PL were analyzed pre-dose and 2, 4, 6, 8, 24, 48, and 72 h after capsule ingestion. Additionally, the proportion of free EPA and DHA in the applied supplements was analyzed. Results: The highest incorporation of EPA+DHA into plasma PL was provoked by krill oil (mean AUC 0-72 h: 80.03 34.71%*h), followed by fish oil rTAG (mean AUC 0-72 h: 59.78 36.75%*h) and EE (mean AUC 0-72 h: 47.53 38.42%*h). Due to high standard deviation values, there were no significant differences for DHA and the sum of EPA+DHA levels between the three treatments. However, a trend (p = 0.057) was observed for the differences in EPA bioavailability. Statistical pair-wise group comparison's revealed a trend (p = 0.086) between rTAG and krill oil. FA analysis of the supplements showed that the krill oil sample contained 22% of the total EPA amount as free EPA and 21% of the total DHA amount as free DHA, while the two fish oil samples did not contain any free FA. Conclusion: Further studies with a larger sample size carried out over a longer period are needed to substantiate our findings and to determine differences in EPA+DHA bioavailability between three common chemical forms of LC n-3 FA (rTAG, EE and krill oil). The unexpected high content of free EPA and DHA in krill oil, which might have a significant influence on the availability of EPA+DHA from krill oil, should be investigated in more depth and taken into consideration in future trials.
Made available in DSpace on 2016-11-02T08:33:43Z (GMT). No. of bitstreams: 0
Previous issue date: 2011
publishedVersion
eng
London : BioMed Central Ltd.
Lipids in Health and Disease 10 (2011)
1476-511X
http://dx.doi.org/10.1186/1476-511X-10-145
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
absorption
bioavailability
ethyl esters
fish oil
krill oil
re-esterified triacylglycerides
uptake
docosahexaenoic acid
edible oil
fish oil
icosapentaenoic acid
krill oil
nko
omega 3 fatty acid
phospholipid
unclassified drug
docosahexaenoic acid
fish oil
icosapentaenoic acid
phospholipid
adult
area under the curve
article
body weight
controlled study
crossover procedure
diet supplementation
double blind procedure
drug bioavailability
drug blood level
drug formulation
human
human experiment
intermethod comparison
male
maximum plasma concentration
normal human
phospholipid blood level
randomized controlled trial
time to maximum plasma concentration
animal
blood
chemistry
clinical trial
comparative study
controlled clinical trial
Euphausiacea
Euphausiacea
Adult
Animals
Area Under Curve
Chemistry, Pharmaceutical
Docosahexaenoic Acids
Double-Blind Method
Eicosapentaenoic Acid
Euphausiacea
Fish Oils
Humans
Male
Phospholipids
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Incorporation of EPA and DHA into plasma phospholipids in response to different omega-3 fatty acid formulations - A comparative bioavailability study of fish oil vs. krill oil
Article
Text
10
145
openAccess
ORIGINAL
art_10.1186_1476-511X-10-145.pdf
art_10.1186_1476-511X-10-145.pdf
application/pdf
485031
https://www.repo.uni-hannover.de/bitstream/123456789/632/1/art_10.1186_1476-511X-10-145.pdf
5c37220400531bcc0fe6f03d1a8cde91
MD5
1
TEXT
art_10.1186_1476-511X-10-145.pdf.txt
art_10.1186_1476-511X-10-145.pdf.txt
Extracted Text
text/plain
34652
https://www.repo.uni-hannover.de/bitstream/123456789/632/2/art_10.1186_1476-511X-10-145.pdf.txt
316ba5c5b88d34228f6dc8ebf1f95d92
MD5
2
THUMBNAIL
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art_10.1186_1476-511X-10-145.pdf.jpg
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image/jpeg
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https://www.repo.uni-hannover.de/bitstream/123456789/632/3/art_10.1186_1476-511X-10-145.pdf.jpg
a136cbca803035df1989405c92b4421b
MD5
3
123456789/632
oai:www.repo.uni-hannover.de:123456789/632
2022-12-02 16:19:58.85
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6332022-12-02T15:19:58Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570
Angrisani, Nina
ccea4e82-63f4-449e-b0c1-9b8b032891ee
600
Foth, Franziska
2e6ab29d-732c-4bc1-a2ff-241b208dfaa2
600
Kietzmann, Manfred
c895953c-18d2-4403-b47f-93b4ec2fb102
600
Schumacher, Stephan
43d74fa0-7753-46cf-91ac-a51bb835990d
600
Angrisani, Gian Luigi
2976c61a-ad45-4c96-b97a-d3132dd7565c
600
Christel, Anne
30d7562b-ff0f-45e5-a1bf-2ec8e9e3114e
600
Behrens, Peter
83509335-eef2-4ca9-a566-48da2c50ed01
600
Reifenrath, Janin
4cd37190-194b-46f4-a59f-f6121ea7e075
600
2016-11-02T08:33:43Z
2016-11-02T08:33:43Z
2013
Angrisani, N.; Foth, F.; Kietzmann, M.; Schumacher, S.; Angrisani, Gian Luigi et al.: Increased accumulation of magnetic nanoparticles by magnetizable implant materials for the treatment of implant-associated complications. In: Journal of Nanobiotechnology 11 (2013), Nr. 1, 34. DOI: http://dx.doi.org/10.1186/1477-3155-11-34
http://www.repo.uni-hannover.de/handle/123456789/633
http://dx.doi.org/10.15488/609
Background: In orthopaedic surgery, accumulation of agents such as anti-infectives in the bone as target tissue is difficult. The use of magnetic nanoparticles (MNPs) as carriers principally enables their accumulation via an externally applied magnetic field. Magnetizable implants are principally able to increase the strength of an externally applied magnetic field to reach also deep-seated parts in the body. Therefore, the integration of bone-addressed therapeutics in MNPs and their accumulation at a magnetic orthopaedic implant could improve the treatment of implant related infections. In this study a martensitic steel platelet as implant placeholder was used to examine its accumulation and retention capacity of MNPs in an in vitro experimental set up considering different experimental frame conditions as magnet quantity and distance to each other, implant thickness and flow velocity.Results: The magnetic field strength increased to approximately 112% when a martensitic stainless steel platelet was located between the magnet poles. Therewith a significantly higher amount of magnetic nanoparticles could be accumulated in the area of the platelet compared to the sole magnetic field. During flushing of the tube system mimicking the in vivo blood flow, the magnetized platelet was able to retain a higher amount of MNPs without an external magnetic field compared to the set up with no mounted platelet during flushing of the system. Generally, a higher flow velocity led to lower amounts of accumulated MNPs. A higher quantity of magnets and a lower distance between magnets led to a higher magnetic field strength. Albeit not significantly the magnetic field strength tended to increase with thicker platelets.Conclusion: A martensitic steel platelet significantly improved the attachment of magnetic nanoparticles in an in vitro flow system and therewith indicates the potential of magnetic implant materials in orthopaedic surgery. The use of a remanent magnetic implant material could improve the efficiency of capturing MNPs especially when the external magnetic field is turned off thus facilitating and prolonging the effect. In this way higher drug levels in the target area might be attained resulting in lower inconveniences for the patient.
Made available in DSpace on 2016-11-02T08:33:43Z (GMT). No. of bitstreams: 0
Previous issue date: 2013
publishedVersion
eng
London : BioMed Central Ltd.
Journal of Nanobiotechnology 11 (2013), Nr. 1
1477-3155
http://dx.doi.org/10.1186/1477-3155-11-34
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Implant directed magnetic drug targeting
In vitro
Magnetic field strength
Martensitic steel
Applied magnetic fields
External magnetic field
In-vitro
Magnetic drug targeting
Magnetic field strengths
Magnetic nano-particles
Magnetic nanoparti cles (MNPs)
Orthopaedic implants
Bone
Magnetic fields
Magnets
Martensitic steel
Nanoparticles
Orthopedics
Platelets
Electromagnetic field effects
magnetic nanoparticle
stainless steel
article
blood flow velocity
cell volume
flow
magnetic field
orthopedic implant
orthopedic surgery
thrombocyte
Animals
Bone Plates
Ferrosoferric Oxide
Humans
Magnetic Fields
Magnetite Nanoparticles
Magnets
Models, Biological
Rheology
Stainless Steel
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Increased accumulation of magnetic nanoparticles by magnetizable implant materials for the treatment of implant-associated complications
Article
Text
1
11
34
openAccess
ORIGINAL
art_10.1186_1477-3155-11-34.pdf
art_10.1186_1477-3155-11-34.pdf
application/pdf
1222740
https://www.repo.uni-hannover.de/bitstream/123456789/633/1/art_10.1186_1477-3155-11-34.pdf
1d94adc023eaea06c29207d1b31d320c
MD5
1
TEXT
art_10.1186_1477-3155-11-34.pdf.txt
art_10.1186_1477-3155-11-34.pdf.txt
Extracted Text
text/plain
49717
https://www.repo.uni-hannover.de/bitstream/123456789/633/2/art_10.1186_1477-3155-11-34.pdf.txt
22b6dc7101f3c78506a1bbbb623422c0
MD5
2
THUMBNAIL
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art_10.1186_1477-3155-11-34.pdf.jpg
Generated Thumbnail
image/jpeg
1648
https://www.repo.uni-hannover.de/bitstream/123456789/633/3/art_10.1186_1477-3155-11-34.pdf.jpg
f71b00028984f82779929b9c59cf8ad4
MD5
3
123456789/633
oai:www.repo.uni-hannover.de:123456789/633
2022-12-02 16:19:58.89
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6352022-12-02T15:19:58Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Kaufmann, Helgard
0fd8b70a-6784-4ba0-98ba-794951288095
600
Qiu, X.
afea833f-4d1c-4cbd-b8e0-29977a75e2cb
600
Wehmeyer, Juliane
5bf8aef8-7da3-4481-b493-2f47a14150c9
600
Debener, Thomas
2aedee57-8d8b-49ca-9c67-1a988b8fba75
600
2016-11-02T08:33:45Z
2016-11-02T08:33:45Z
2012
Kaufmann, Helgard; Qiu, X.; Wehmeyer, Juliane; Debener, Thomas: Isolation, molecular characterization, and mapping of four rose MLO orthologs. In: Frontiers in Plant Science 3 (2012), 244. DOI: http://dx.doi.org/10.3389/fpls.2012.00244
http://www.repo.uni-hannover.de/handle/123456789/635
http://dx.doi.org/10.15488/611
Powdery mildew is a major disease of economic importance in cut and pot roses. As an alternative to conventional resistance breeding strategies utilizing single-dominant genes or QTLs, mildew resistance locus o (MLO)-based resistance might offer some advantages. In dicots such as Arabidopsis, pea, and tomato, loss-of-function mutations in MLO genes confer high levels of broad-spectrum resistance. Here, we report the isolation and characterization of four MLO homologs from a large rose EST collection isolated from leaves. These genes are phylogenetically closely related to other dicot MLO genes that are involved in plant powdery mildew interactions. Therefore, they are candidates for MLO genes involved in rose powdery mildew interactions. Two of the four isolated genes contain all of the sequence signatures considered to be diagnostic for MLO genes. We mapped all four genes to three linkage groups and conducted the first analysis of alternative alleles. This information is discussed in regards to a reverse genetics approach aimed at the selection of rose plants that are homozygous for loss-of-function in one or more MLO genes.
Made available in DSpace on 2016-11-02T08:33:45Z (GMT). No. of bitstreams: 0
Previous issue date: 2012
BMBF/0315542A
Natural Science Foundation of China/No31160403
Natural Science Foundation of Yunnan/2011FB124
publishedVersion
eng
Lausanne : Frontiers Research Foundation
Frontiers in Plant Science 3 (2012)
1664-462X
http://dx.doi.org/10.3389/fpls.2012.00244
CC BY 3.0 Unported
http://creativecommons.org/licenses/by/3.0/
Mildew resistance locus o
Podosphaera pannosa
Powdery mildew
Rosaceae
Tetraploid
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Isolation, molecular characterization, and mapping of four rose MLO orthologs
Article
Text
3
244
openAccess
ORIGINAL
fpls-03-00244.pdf
fpls-03-00244.pdf
application/pdf
1066563
https://www.repo.uni-hannover.de/bitstream/123456789/635/1/fpls-03-00244.pdf
53912da6d848285383fa1832347be484
MD5
1
TEXT
fpls-03-00244.pdf.txt
fpls-03-00244.pdf.txt
Extracted Text
text/plain
66190
https://www.repo.uni-hannover.de/bitstream/123456789/635/2/fpls-03-00244.pdf.txt
c48b875a62c2f1ad3db06675e4670ad5
MD5
2
THUMBNAIL
fpls-03-00244.pdf.jpg
fpls-03-00244.pdf.jpg
Generated Thumbnail
image/jpeg
1584
https://www.repo.uni-hannover.de/bitstream/123456789/635/3/fpls-03-00244.pdf.jpg
eecc4589b99d246b9a85432fc957bcbc
MD5
3
123456789/635
oai:www.repo.uni-hannover.de:123456789/635
2022-12-02 16:19:58.872
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6432022-12-02T16:11:40Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Santiago, Julia
33cc90c4-90ba-4680-abf5-615a382a0654
600
Brandt, Benjamin
501a0a2d-49c4-4917-8b4f-269440659b00
600
Wildhagen, Mari
f247b71b-768f-4fc9-90a3-d7fd27102e8c
600
Hohmann, Ulrich
d0a9d867-f777-422a-a8e6-482294466459
600
Hothorn, Ludwig A.
2bb8348e-4c4a-4642-a1ee-ec310b3fd6e4
600
Butenko, Melinka A.
0a7bef82-ba12-4aae-a0da-9588b8df8b3d
600
Hothorn, Michael
f9566aa3-d32d-4b23-b574-76c0fb6fb7e7
600
2016-11-02T08:33:59Z
2016-11-02T08:33:59Z
2016
Santiago, J.; Brandt, B.; Wildhagen, M.; Hohmann, U.; Hothorn, Ludwig A. et al.: Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission. In: eLife 5 (2016), e15075. DOI: http://dx.doi.org/10.7554/eLife.15075
http://www.repo.uni-hannover.de/handle/123456789/643
http://dx.doi.org/10.15488/619
Plants constantly renew during their life cycle and thus require to shed senescent and damaged organs. Floral abscission is controlled by the leucine-rich repeat receptor kinase (LRR-RK) HAESA and the peptide hormone IDA. It is unknown how expression of IDA in the abscission zone leads to HAESA activation. Here we show that IDA is sensed directly by the HAESA ectodomain. Crystal structures of HAESA in complex with IDA reveal a hormone binding pocket that accommodates an active dodecamer peptide. A central hydroxyproline residue anchors IDA to the receptor. The HAESA co-receptor SERK1, a positive regulator of the floral abscission pathway, allows for high-affinity sensing of the peptide hormone by binding to an Arg-His-Asn motif in IDA. This sequence pattern is conserved among diverse plant peptides, suggesting that plant peptide hormone receptors may share a common ligand binding mode and activation mechanism.
Made available in DSpace on 2016-11-02T08:33:59Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
Swiss National Science Foundation/31003A_156920
Research Council of Norway/13785/F20
Research Council of Norway/230849/F20
Research Council of Norway/348256/F20
publishedVersion
eng
Castle Park : eLife Sciences Publications Ltd
eLife 5 (2016)
2050-084X
http://dx.doi.org/10.7554/eLife.15075
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
biophysics and structural biology
plant biology
A. thaliana
floral abscission
membrane signaling
peptide hormone
plant development
protein complexes
receptor kinase
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission
Article
Text
5
e15075
openAccess
ORIGINAL
e15075-download.pdf
e15075-download.pdf
application/pdf
4703899
https://www.repo.uni-hannover.de/bitstream/123456789/643/1/e15075-download.pdf
7ab600abbed0c759fe1abb3d2435cec9
MD5
1
elife15075-figures-1.pdf
elife15075-figures-1.pdf
application/pdf
7796062
https://www.repo.uni-hannover.de/bitstream/123456789/643/2/elife15075-figures-1.pdf
4e5969417080113d779874588babf34a
MD5
2
TEXT
e15075-download.pdf.txt
e15075-download.pdf.txt
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text/plain
63458
https://www.repo.uni-hannover.de/bitstream/123456789/643/3/e15075-download.pdf.txt
17a7f6a56f4db9582732d1c22e115b9a
MD5
3
elife15075-figures-1.pdf.txt
elife15075-figures-1.pdf.txt
Extracted Text
text/plain
16931
https://www.repo.uni-hannover.de/bitstream/123456789/643/5/elife15075-figures-1.pdf.txt
a8b682557d80ed89300adac2b53d644b
MD5
5
THUMBNAIL
e15075-download.pdf.jpg
e15075-download.pdf.jpg
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image/jpeg
1725
https://www.repo.uni-hannover.de/bitstream/123456789/643/4/e15075-download.pdf.jpg
e1cfb3f14c5c6e0e78b70f0373a7c24c
MD5
4
elife15075-figures-1.pdf.jpg
elife15075-figures-1.pdf.jpg
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https://www.repo.uni-hannover.de/bitstream/123456789/643/6/elife15075-figures-1.pdf.jpg
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MD5
6
123456789/643
oai:www.repo.uni-hannover.de:123456789/643
2022-12-02 17:11:40.908
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6442022-12-02T15:04:49Zcom_123456789_1col_123456789_4doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570
Grothausmann, Roman
91d9dea4-5460-4649-96fc-104ce9dd4c89
600
Kellner, Manuela
2000eac9-1d1c-49d9-9b6d-aba2ab859031
600
Heidrich, Marko
ee4cfb2e-b310-4f1e-9ce5-fdc2f46430c8
600
Lorbeer, Raoul-Amadeus
d8bffbb9-f29a-4851-bed3-6c5f7c4c3fe6
600
Ripken, Tammo
8b984aa6-f2cc-4c07-8639-b93a2557080c
600
Meyer, Heiko
6e649280-5cdd-4c88-afee-c0e1002be521
600
Kuehnel, Mark P.
193c2cfb-0edd-48e3-a009-991370be1623
600
Ochs, Matthias
fdac4976-83a4-4736-a11b-96061babe9c5
600
Rosenhahn, Bodo
3322fcea-8075-4df2-bf83-0c8c50b024c1
600
2016-11-02T13:35:58Z
2016-11-02T13:35:58Z
2015
Grothausmann, R.; Kellner, M.; Heidrich, M.; Lorbeer, R.-A.; Ripken, T. et al.: Method for 3D airway topology extraction. In: Computational and Mathematical Methods in Medicine 2015 (2015), 127010. DOI: http://dx.doi.org/10.1155/2015/127010
http://www.repo.uni-hannover.de/handle/123456789/644
http://dx.doi.org/10.15488/620
In lungs the number of conducting airway generations as well as bifurcation patterns varies across species and shows specific characteristics relating to illnesses or gene variations. A method to characterize the topology of the mouse airway tree using scanning laser optical tomography (SLOT) tomograms is presented in this paper. It is used to test discrimination between two types of mice based on detected differences in their conducting airway pattern. Based on segmentations of the airways in these tomograms, the main spanning tree of the volume skeleton is computed. The resulting graph structure is used to distinguish between wild type and surfactant protein (SP-D) deficient knock-out mice.
Made available in DSpace on 2016-11-02T13:35:58Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
DFG/Oc 23/9-3
DFG/Oc23/10-1
DFG/REBIRTH
Swiss National Science Foundation (SNF)
National Institutes of Health
BMBF/DZL
publishedVersion
eng
New York, NY : Hindawi Publishing Corporation
Computational and Mathematical Methods in Medicine 2015 (2015)
1748-670X
http://dx.doi.org/10.1155/2015/127010
CC BY 3.0 Unported
https://creativecommons.org/licenses/by/3.0/
surfactant protein D
surfactant protein D
absorption
airway conductance
animal tissue
Article
controlled study
fluorescence
genetic variability
mouse
nonhuman
optical tomography
scanning laser optical tomography
tracheobronchial tree
algorithm
anatomic model
animal
bronchus
C57BL mouse
chemistry
computer assisted tomography
image processing
knockout mouse
lung
multimodal imaging
optics
physiology
procedures
trachea
Mus
Algorithms
Animals
Bronchi
Image Processing, Computer-Assisted
Lung
Mice
Mice, Inbred C57BL
Mice, Knockout
Models, Anatomic
Multimodal Imaging
Optics and Photonics
Pulmonary Surfactant-Associated Protein D
Tomography, X-Ray Computed
Trachea
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Method for 3D airway topology extraction
Article
Text
2015
127010
openAccess
ORIGINAL
127010.pdf
127010.pdf
application/pdf
3836366
https://www.repo.uni-hannover.de/bitstream/123456789/644/1/127010.pdf
1feaadcdcba1968f4e3ae4a22090bd08
MD5
1
TEXT
127010.pdf.txt
127010.pdf.txt
Extracted Text
text/plain
30036
https://www.repo.uni-hannover.de/bitstream/123456789/644/2/127010.pdf.txt
fee4b705d737d77ee2a1aaee03d4de8f
MD5
2
THUMBNAIL
127010.pdf.jpg
127010.pdf.jpg
Generated Thumbnail
image/jpeg
1543
https://www.repo.uni-hannover.de/bitstream/123456789/644/3/127010.pdf.jpg
6c793c5de55e23174440430274322ea1
MD5
3
123456789/644
oai:www.repo.uni-hannover.de:123456789/644
2022-12-02 16:04:49.355
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6472022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Hothorn, Ludwig A.
2bb8348e-4c4a-4642-a1ee-ec310b3fd6e4
600
Libiger, Ondrej
6b58a070-4d6a-4bb0-af86-5163fb8d95d8
600
Gerhard, Daniel
3d5a32da-af99-44d8-bc42-bc12668aa2a0
600
2016-11-02T13:36:02Z
2016-11-02T13:36:02Z
2012
Hothorn, Ludwig A.; Libiger, O.; Gerhard, Daniel: Model-specific tests on variance heterogeneity for detection of potentially interacting genetic loci. In: BMC Genetics 13 (2012), 59. DOI: http://dx.doi.org/10.1186/1471-2156-13-59
http://www.repo.uni-hannover.de/handle/123456789/647
http://dx.doi.org/10.15488/623
Background: Trait variances among genotype groups at a locus are expected to differ in the presence of an interaction between this locus and another locus or environment. A simple maximum test on variance heterogeneity can thus be used to identify potentially interacting single nucleotide polymorphisms (SNPs).Results: We propose a multiple contrast test for variance heterogeneity that compares the mean of Levene residuals for each genotype group with their average as an alternative to a global Levene test. We applied this test to a Bogalusa Heart Study dataset to screen for potentially interacting SNPs across the whole genome that influence a number of quantitative traits. A user-friendly implementation of this method is available in the R statistical software package multcomp.Conclusions: We show that the proposed multiple contrast test of model-specific variance heterogeneity can be used to test for potential interactions between SNPs and unknown alleles, loci or covariates and provide valuable additional information compared with traditional tests. Although the test is statistically valid for severely unbalanced designs, care is needed in interpreting the results at loci with low allele frequencies.
Made available in DSpace on 2016-11-02T13:36:02Z (GMT). No. of bitstreams: 0
Previous issue date: 2012
DFG/HO1687
publishedVersion
eng
London : BioMed Central Ltd.
BMC Genetics 13 (2012)
1471-2156
http://dx.doi.org/10.1186/1471-2156-13-59
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Genetic association study
Interaction
Quantitative traits
Variance heterogeneity
article
computer program
gene locus
genetic association
genome
genotype
human
quantitative trait
single nucleotide polymorphism
Alleles
Analysis of Variance
Blood Pressure
Gene Frequency
Genetic Heterogeneity
Genome-Wide Association Study
Genotype
Heart Diseases
Humans
Models, Genetic
Phenotype
Polymorphism, Single Nucleotide
Quantitative Trait Loci
Software
Waist Circumference
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Model-specific tests on variance heterogeneity for detection of potentially interacting genetic loci
Article
Text
13
59
openAccess
ORIGINAL
art_10.1186_1471-2156-13-59.pdf
art_10.1186_1471-2156-13-59.pdf
application/pdf
491375
https://www.repo.uni-hannover.de/bitstream/123456789/647/1/art_10.1186_1471-2156-13-59.pdf
76bdf99f4647ac788b3bd6ae31467762
MD5
1
TEXT
art_10.1186_1471-2156-13-59.pdf.txt
art_10.1186_1471-2156-13-59.pdf.txt
Extracted Text
text/plain
23950
https://www.repo.uni-hannover.de/bitstream/123456789/647/2/art_10.1186_1471-2156-13-59.pdf.txt
82c401fa9203e61013a6153353d25ad8
MD5
2
THUMBNAIL
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art_10.1186_1471-2156-13-59.pdf.jpg
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1731
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20832557b9d737e51841929ad471d167
MD5
3
123456789/647
oai:www.repo.uni-hannover.de:123456789/647
2022-12-02 17:14:10.13
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6492022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Göhler, Anna-Katharina
e21c98d8-ce55-42e1-aaa8-de9a8539e5f0
600
Kökpinar, Öznur
2017b62a-2d8e-44c4-a552-2c4760cd23cb
600
Schmidt-Heck, Wolfgang
ac96f8ab-48e9-4d63-a0da-7a884f587371
600
Geffers, Robert
efee96fe-03cf-4c0c-82fa-7aeeeb27683e
600
Guthke, Reinhard
84fbddc1-10aa-4307-ab3d-06ea29d3118e
600
Rinas, Ursula
7399be1d-6517-4855-8bdf-d054fa56ebaa
600
Schuster, Stefan
45695412-6a5a-4cda-b3e1-8c24d91833b3
600
Jahreis, Knut
17764308-3f05-4499-977b-f02bddb41b4b
600
Kaleta, Christoph
3e82b314-435c-4806-81b7-bd6ece3598dc
600
2016-11-02T13:36:02Z
2016-11-02T13:36:02Z
2011
Göhler, A.-K.; Kökpinar, Öznur; Schmidt-Heck, W.; Geffers, R.; Guthke, R. et al.: More than just a metabolic regulator - elucidation and validation of new targets of PdhR in Escherichia coli. In: BMC Systems Biology 5 (2011), 197. DOI: http://dx.doi.org/10.1186/1752-0509-5-197
http://www.repo.uni-hannover.de/handle/123456789/649
http://dx.doi.org/10.15488/625
Background: The pyruvate dehydrogenase regulator protein (PdhR) of Escherichia coli acts as a transcriptional regulator in a pyruvate dependent manner to control central metabolic fluxes. However, the complete PdhR regulon has not yet been uncovered. To achieve an extended understanding of its gene regulatory network, we combined large-scale network inference and experimental verification of results obtained by a systems biology approach.Results: 22 new genes contained in two operons controlled by PdhR (previously only 20 regulatory targets in eight operons were known) were identified by analysing a large-scale dataset of E. coli from the Many Microbes Microarray Database and novel expression data from a pdhR knockout strain, as well as a PdhR overproducing strain. We identified a regulation of the glycolate utilization operon glcDEFGBA using chromatin immunoprecipitation and gel shift assays. We show that this regulation could be part of a cross-induction between genes necessary for acetate and pyruvate utilisation controlled through PdhR. Moreover, a link of PdhR regulation to the replication machinery of the cell via control of the transcription of the dcw-cluster was verified in experiments. This augments our knowledge of the functions of the PdhR-regulon and demonstrates its central importance for further cellular processes in E. coli.Conclusions: We extended the PdhR regulon by 22 new genes contained in two operons and validated the regulation of the glcDEFGBA operon for glycolate utilisation and the dcw-cluster for cell division proteins experimentally. Our results provide, for the first time, a plausible regulatory link between the nutritional status of the cell and cell replication mediated by PdhR.
Made available in DSpace on 2016-11-02T13:36:02Z (GMT). No. of bitstreams: 0
Previous issue date: 2011
BMBF/FKZ/0315285
publishedVersion
eng
London : BioMed Central Ltd.
BMC Systems Biology 5 (2011)
1752-0509
http://dx.doi.org/10.1186/1752-0509-5-197
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Escherichia coli
Escherichia coli protein
PdhR protein, E coli
repressor protein
article
bacterial gene
Escherichia coli
gene expression regulation
gene regulatory network
genetics
metabolism
methodology
physiology
regulon
systems biology
Escherichia coli
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
Gene Regulatory Networks
Genes, Bacterial
Metabolic Networks and Pathways
Regulon
Repressor Proteins
Systems Biology
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
More than just a metabolic regulator - elucidation and validation of new targets of PdhR in Escherichia coli
Article
Text
5
197
openAccess
ORIGINAL
art_10.1186_1752-0509-5-197.pdf
art_10.1186_1752-0509-5-197.pdf
application/pdf
976482
https://www.repo.uni-hannover.de/bitstream/123456789/649/1/art_10.1186_1752-0509-5-197.pdf
27c1290b19d94843291e0b695d4daa74
MD5
1
TEXT
art_10.1186_1752-0509-5-197.pdf.txt
art_10.1186_1752-0509-5-197.pdf.txt
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text/plain
53805
https://www.repo.uni-hannover.de/bitstream/123456789/649/2/art_10.1186_1752-0509-5-197.pdf.txt
52a8c292a3a0f6db863175d86d022b3e
MD5
2
THUMBNAIL
art_10.1186_1752-0509-5-197.pdf.jpg
art_10.1186_1752-0509-5-197.pdf.jpg
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image/jpeg
1686
https://www.repo.uni-hannover.de/bitstream/123456789/649/3/art_10.1186_1752-0509-5-197.pdf.jpg
0edc57ffdd33e2c0ab839078ce6cdd21
MD5
3
123456789/649
oai:www.repo.uni-hannover.de:123456789/649
2022-12-02 17:14:10.12
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6532022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Frömke, C.
ea4cc41f-c317-478d-92c0-9e3c5bcff95a
600
Hothorn, Ludwig A.
2bb8348e-4c4a-4642-a1ee-ec310b3fd6e4
600
Kropf, S.
13e4ccfe-aefb-43cc-8cbd-0656c9584874
600
2016-11-02T13:36:03Z
2016-11-02T13:36:03Z
2008
Frömke, C.; Hothorn, Ludwig, A.; Kropf, S.: Nonparametric relevance-shifted multiple testing procedures for the analysis of high-dimensional multivariate data with small sample sizes. In: BMC Bioinformatics 9 (2008), 54. DOI. http://dx.doi.org/10.1186/1471-2105-9-54
http://www.repo.uni-hannover.de/handle/123456789/653
http://dx.doi.org/10.15488/629
Background: In many research areas it is necessary to find differences between treatment groups with several variables. For example, studies of microarray data seek to find a significant difference in location parameters from zero or one for ratios thereof for each variable. However, in some studies a significant deviation of the difference in locations from zero (or 1 in terms of the ratio) is biologically meaningless. A relevant difference or ratio is sought in such cases. Results: This article addresses the use of relevance-shifted tests on ratios for a multivariate parallel two-sample group design. Two empirical procedures are proposed which embed the relevance-shifted test on ratios. As both procedures test a hypothesis for each variable, the resulting multiple testing problem has to be considered. Hence, the procedures include a multiplicity correction. Both procedures are extensions of available procedures for point null hypotheses achieving exact control of the familywise error rate. Whereas the shift of the null hypothesis alone would give straight-forward solutions, the problems that are the reason for the empirical considerations discussed here arise by the fact that the shift is considered in both directions and the whole parameter space in between these two limits has to be accepted as null hypothesis. Conclusion: The first algorithm to be discussed uses a permutation algorithm, and is appropriate for designs with a moderately large number of observations. However, many experiments have limited sample sizes. Then the second procedure might be more appropriate, where multiplicity is corrected according to a concept of data-driven order of hypotheses.
Made available in DSpace on 2016-11-02T13:36:03Z (GMT). No. of bitstreams: 0
Previous issue date: 2008
publishedVersion
eng
London : BioMed Central Ltd.
BMC Bioinformatics 9 (2008)
1471-2105
http://dx.doi.org/10.1186/1471-2105-9-54
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Familywise error rate
Location parameters
Multiple testing problems
Multivariate data
Parameter spaces
Second procedures
Several variables
Small Sample Size
Algorithms
Statistical tests
analytical error
article
data analysis
mathematical analysis
mathematical computing
medical research
methodology
microarray analysis
multivariate analysis
nonparametric test
null hypothesis
rank sum test
sample size
simulation
statistical significance
algorithm
biological model
computer simulation
DNA microarray
gene expression profiling
sample size
signal processing
statistical analysis
statistical model
Algorithms
Computer Simulation
Data Interpretation, Statistical
Gene Expression Profiling
Models, Biological
Models, Statistical
Multivariate Analysis
Oligonucleotide Array Sequence Analysis
Sample Size
Signal Processing, Computer-Assisted
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Nonparametric relevance-shifted multiple testing procedures for the analysis of high-dimensional multivariate data with small sample sizes
Article
Text
9
54
openAccess
ORIGINAL
art_10.1186_1471-2105-9-54.pdf
art_10.1186_1471-2105-9-54.pdf
application/pdf
338934
https://www.repo.uni-hannover.de/bitstream/123456789/653/1/art_10.1186_1471-2105-9-54.pdf
1b8f7776e89cc9630456f414412b5bdd
MD5
1
TEXT
art_10.1186_1471-2105-9-54.pdf.txt
art_10.1186_1471-2105-9-54.pdf.txt
Extracted Text
text/plain
54073
https://www.repo.uni-hannover.de/bitstream/123456789/653/2/art_10.1186_1471-2105-9-54.pdf.txt
a054bf84598e4977752c66738eae3b7f
MD5
2
THUMBNAIL
art_10.1186_1471-2105-9-54.pdf.jpg
art_10.1186_1471-2105-9-54.pdf.jpg
Generated Thumbnail
image/jpeg
1767
https://www.repo.uni-hannover.de/bitstream/123456789/653/3/art_10.1186_1471-2105-9-54.pdf.jpg
740e4c95832e0eae993a762229c34eb7
MD5
3
123456789/653
oai:www.repo.uni-hannover.de:123456789/653
2022-12-02 17:14:10.091
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6572022-12-02T15:17:13Zcom_123456789_1col_123456789_7doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570
Krawinkel, Judith
ec337106-fafa-445c-aa31-b2a355979a28
600
Richter, Undine
103a33ef-66b1-47ed-a888-fe7fd20def81
600
Torres-Mapa, Maria Leilani
a151d4dc-67ed-4d2e-a8dc-4c8326f03fbf
600
Westermann, Martin
fdd544d9-49e5-47c0-882b-da8a52335fea
600
Gamrad, Lisa
5d812662-2400-4b48-badf-848280daf668
600
Rehbock, Christoph
6a9fbf77-c20c-4145-85d3-daab1565233f
600
Barcikowski, Stephan
1ed871d2-540f-40a9-b0a7-5c9677174ae3
600
Heisterkamp, Alexander
67fb34de-b181-4db7-b626-59482982c9b3
600
2016-11-03T09:29:49Z
2016-11-03T09:29:49Z
2016
Krawinkel, J.; Richter, U.; Torres-Mapa, Maria Leilani; Westermann, M.; Gamrad, L. et al.: Optical and electron microscopy study of laser-based intracellular molecule delivery using peptide-conjugated photodispersible gold nanoparticle agglomerates. In: Journal of Nanobiotechnology 14 (2016), Nr. 1, 2. DOI: http://dx.doi.org/10.1186/s12951-015-0155-8
http://www.repo.uni-hannover.de/handle/123456789/657
http://dx.doi.org/10.15488/633
Background: Cell-penetrating peptides (CPPs) can act as carriers for therapeutic molecules such as drugs and genetic constructs for medical applications. The triggered release of the molecule into the cytoplasm can be crucial to its effective delivery. Hence, we implemented and characterized laser interaction with defined gold nanoparticle agglomerates conjugated to CPPs which enables efficient endosomal rupture and intracellular release of molecules transported. Results: Gold nanoparticles generated by pulsed laser ablation in liquid were conjugated with CPPs forming agglomerates and the intracellular release of molecules was triggered via pulsed laser irradiation (λ = 532 nm, τpulse = 1 ns). The CPPs enhance the uptake of the agglomerates along with the cargo which can be co-incubated with the agglomerates. The interaction of incident laser light with gold nanoparticle agglomerates leads to heat deposition and field enhancement in the vicinity of the particles. This highly precise effect deagglomerates the nanoparticles and disrupts the enclosing endosomal membrane. Transmission electron microscopy images confirmed this rupture for radiant exposures of 25 mJ/cm2 and above. Successful intracellular release was shown using the fluorescent dye calcein. For a radiant exposure of 35 mJ/cm2 we found calcein delivery in 81 % of the treated cells while maintaining a high percentage of cell viability. Furthermore, cell proliferation and metabolic activity were not reduced 72 h after the treatment. Conclusion: CPPs trigger the uptake of the gold nanoparticle agglomerates via endocytosis and co-resident molecules in the endosomes are released by applying laser irradiation, preventing their intraendosomal degradation. Due to the highly localized effect, the cell membrane integrity is not affected. Therefore, this technique can be an efficient tool for spatially and temporally confined intracellular release. The utilization of specifically designed photodispersible gold nanoparticle agglomerates (65 nm) can open novel avenues in imaging and molecule delivery. Due to the induced deagglomeration the primary, small particles (~5 nm) are more likely to be removed from the body.
Made available in DSpace on 2016-11-03T09:29:49Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
DFG/Ba3580/10
publishedVersion
eng
London : BioMed Central Ltd.
Journal of Nanobiotechnology 14 (2016), Nr. 1
1477-3155
http://dx.doi.org/10.1186/s12951-015-0155-8
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
Cell-penetrating peptides
Endosomes
Gold nanoparticles
Intracellular molecule delivery
Laser-based release
Particle agglomerates
Agglomeration
Cell proliferation
Cells
Cytology
Electron microscopy
Fiber optic sensors
Gold
High resolution transmission electron microscopy
Irradiation
Laser ablation
Medical applications
Metal nanoparticles
Molecular biology
Molecules
Nanoparticles
Peptides
Transmission electron microscopy
Cell-penetrating peptide
Endosomes
Gold Nanoparticles
Intracellular molecule delivery
Laser-based
Particle agglomerates
Pulsed lasers
calcein
cell penetrating peptide
gold nanoparticle
animal cell
Article
cell proliferation
cell viability
cellular distribution
conjugation
controlled study
cytoplasm
drug cytotoxicity
electron microscopy
endocytosis
endosome
laser
membrane damage
microscopy
nonhuman
transmission electron microscopy
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Optical and electron microscopy study of laser-based intracellular molecule delivery using peptide-conjugated photodispersible gold nanoparticle agglomerates
Article
Text
1
14
2
openAccess
ORIGINAL
art_10.1186_s12951-015-0155-8.pdf
art_10.1186_s12951-015-0155-8.pdf
application/pdf
3105733
https://www.repo.uni-hannover.de/bitstream/123456789/657/1/art_10.1186_s12951-015-0155-8.pdf
af6bee54faf9edeb8da1468c774ecd70
MD5
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TEXT
art_10.1186_s12951-015-0155-8.pdf.txt
art_10.1186_s12951-015-0155-8.pdf.txt
Extracted Text
text/plain
67010
https://www.repo.uni-hannover.de/bitstream/123456789/657/2/art_10.1186_s12951-015-0155-8.pdf.txt
c473c0a9f4bb469e798198c9f371b5dd
MD5
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THUMBNAIL
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art_10.1186_s12951-015-0155-8.pdf.jpg
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image/jpeg
1706
https://www.repo.uni-hannover.de/bitstream/123456789/657/3/art_10.1186_s12951-015-0155-8.pdf.jpg
818ce0b848eca4fb166260ca6f4c7804
MD5
3
123456789/657
oai:www.repo.uni-hannover.de:123456789/657
2022-12-02 16:17:13.535
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6592022-12-02T15:06:08Zcom_123456789_1col_123456789_6doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570ddc:600
Duda, Sven
36f946d8-d66e-4a76-8b0e-cd0ea30487e2
600
Dreyer, Lutz
02ce2a11-93f7-44b1-a937-141d1d465dc8
600
Behrens, Peter
83509335-eef2-4ca9-a566-48da2c50ed01
600
Wienecke, Soenke
1150a299-5d05-400e-93f0-c1fd73559225
600
Chakradeo, Tanmay
782b8ef6-2361-49cd-9347-208e5db63ff5
600
Glasmacher, Birgit
9b308445-91c6-463b-80d9-d0ec257691d2
600
Haastert-Talini, Kirsten
900ae1a6-2e1d-4fe7-ba1e-02b6c79e2231
600
2016-11-03T09:29:49Z
2016-11-03T09:29:49Z
2014
Duda, S.; Dreyer, Lutz; Behrens Peter; Wienecke, Soenke; Chakradeo, Tanmay et al.: Outer electrospun polycaprolactone shell induces massive foreign body reaction and impairs axonal regeneration through 3D multichannel chitosan nerve guides. In: BioMed Research International 2014 (2014), 835269. DOI: http://dx.doi.org/10.1155/2014/835269
http://www.repo.uni-hannover.de/handle/123456789/659
http://dx.doi.org/10.15488/635
We report on the performance of composite nerve grafts with an inner 3D multichannel porous chitosan core and an outer electrospun polycaprolactone shell. The inner chitosan core provided multiple guidance channels for regrowing axons. To analyze the in vivo properties of the bare chitosan cores, we separately implanted them into an epineural sheath. The effects of both graft types on structural and functional regeneration across a 10 mm rat sciatic nerve gap were compared to autologous nerve transplantation (ANT). The mechanical biomaterial properties and the immunological impact of the grafts were assessed with histological techniques before and after transplantation in vivo. Furthermore during a 13-week examination period functional tests and electrophysiological recordings were performed and supplemented by nerve morphometry. The sheathing of the chitosan core with a polycaprolactone shell induced massive foreign body reaction and impairment of nerve regeneration. Although the isolated novel chitosan core did allow regeneration of axons in a similar size distribution as the ANT, the ANT was superior in terms of functional regeneration. We conclude that an outer polycaprolactone shell should not be used for the purpose of bioartificial nerve grafting, while 3D multichannel porous chitosan cores could be candidate scaffolds for structured nerve grafts.
Made available in DSpace on 2016-11-03T09:29:49Z (GMT). No. of bitstreams: 0
Previous issue date: 2014
Internationale Stiftung Neurobionik, Germany
publishedVersion
eng
New York, NY : Hindawi Publishing Corporation
BioMed Research International 2014 (2014)
2314-6133
http://dx.doi.org/10.1155/2014/835269
CC BY 3.0 Unported
https://creativecommons.org/licenses/by/3.0/
chitosan
polycaprolactone
biomaterial
chitosan
polycaprolactone
polyester
adult
animal experiment
article
automutilation
controlled study
electric field
electrophysiological procedures
electrospinning
extracellular space
female
foreign body reaction
freeze drying
giant cell
in vivo study
microenvironment
nerve fiber regeneration
nerve transplantation
nociception
nonhuman
peripheral nerve
rat
scanning electron microscopy
sciatic nerve
animal
chemically induced
convalescence
drug effects
electrodiagnosis
foreign body reaction
inflammation
motor activity
muscle
nerve fiber
nerve regeneration
organ size
pathology
pathophysiology
procedures
tissue regeneration
tissue scaffold
Wistar rat
Animals
Axons
Biocompatible Materials
Chitosan
Electrodiagnosis
Female
Foreign-Body Reaction
Guided Tissue Regeneration
Inflammation
Microscopy, Electron, Scanning
Motor Activity
Muscles
Nerve Regeneration
Organ Size
Pain Perception
Polyesters
Rats, Wistar
Recovery of Function
Tissue Scaffolds
Dewey Decimal Classification::600 | Technik
600
600
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Outer electrospun polycaprolactone shell induces massive foreign body reaction and impairs axonal regeneration through 3D multichannel chitosan nerve guides
Article
Text
2014
835269
openAccess
ORIGINAL
835269.pdf
835269.pdf
application/pdf
5873939
https://www.repo.uni-hannover.de/bitstream/123456789/659/1/835269.pdf
f1d953010a3fd1f516d69868673be04e
MD5
1
TEXT
835269.pdf.txt
835269.pdf.txt
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73935
https://www.repo.uni-hannover.de/bitstream/123456789/659/2/835269.pdf.txt
3134127eab0a5b3d7eb57cd3cc3f0652
MD5
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835269.pdf.jpg
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1545
https://www.repo.uni-hannover.de/bitstream/123456789/659/3/835269.pdf.jpg
01b455d484d96d05258165dbd04d7252
MD5
3
123456789/659
oai:www.repo.uni-hannover.de:123456789/659
2022-12-02 16:06:08.499
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6602022-12-02T16:14:09Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Villaverde, Antonio
dff5add0-c2f6-46e3-9653-c92d80ca3eab
600
García-Fruitós, Elena
8d581785-169f-42b8-ae92-7bfcee3f5d8b
600
Rinas, Ursula
7399be1d-6517-4855-8bdf-d054fa56ebaa
600
Seras-Franzoso, Joaquin
56e62755-5c73-416e-8a6c-e5f70a0e8281
600
Kosoy, Ana
afce2d05-0146-43e5-a913-190573d22556
600
Corchero, Josè Luis
1aeebab0-f259-4c55-b070-1615c3a5e61f
600
Vázquez, Esther
aff29e5b-df7c-4785-a355-456dd7fbfe56
600
2016-11-03T09:29:50Z
2016-11-03T09:29:50Z
2012
Villaverde, A.; García-Fruitós, E.; Rinas, Ursula; Seras-Franzoso, J.; Kosoy, A. et al.: Packaging protein drugs as bacterial inclusion bodies for therapeutic applications. In: Microbial Cell Factories 11 (2012), 76. DOI: http://dx.doi.org/10.1186/1475-2859-11-76
http://www.repo.uni-hannover.de/handle/123456789/660
http://dx.doi.org/10.15488/636
A growing number of insights on the biology of bacterial inclusion bodies (IBs) have revealed intriguing utilities of these protein particles. Since they combine mechanical stability and protein functionality, IBs have been already exploited in biocatalysis and explored for bottom-up topographical modification in tissue engineering. Being fully biocompatible and with tuneable bio-physical properties, IBs are currently emerging as agents for protein delivery into mammalian cells in protein-replacement cell therapies. So far, IBs formed by chaperones (heat shock protein 70, Hsp70), enzymes (catalase and dihydrofolate reductase), grow factors (leukemia inhibitory factor, LIF) and structural proteins (the cytoskeleton keratin 14) have been shown to rescue exposed cells from a spectrum of stresses and restore cell functions in absence of cytotoxicity. The natural penetrability of IBs into mammalian cells (reaching both cytoplasm and nucleus) empowers them as an unexpected platform for the controlled delivery of essentially any therapeutic polypeptide. Production of protein drugs by biopharma has been traditionally challenged by IB formation. However, a time might have arrived in which recombinant bacteria are to be engineered for the controlled packaging of therapeutic proteins as nanoparticulate materials (nanopills), for their extra- or intra-cellular release in medicine and cosmetics.
Made available in DSpace on 2016-11-03T09:29:50Z (GMT). No. of bitstreams: 0
Previous issue date: 2012
MICINN/BFU2010-17450
AGAUR/2009SGR-108
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN)
publishedVersion
eng
London : BioMed Central Ltd.
Microbial Cell Factories 11 (2012)
1475-2859
http://dx.doi.org/10.1186/1475-2859-11-76
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
catalase
cytokeratin 14
dihydrofolate reductase
heat shock protein 70
leukemia inhibitory factor
nanoparticle
protein
bacterial inclusion body
biocatalysis
biocompatibility
cell function
cell inclusion
cell membrane permeability
cell therapy
cytoplasm
drug delivery system
drug packaging
nonhuman
note
nucleus accumbens
protein degradation
protein folding
protein transport
Bacteria
Catalase
Drug Delivery Systems
HeLa Cells
HSP70 Heat-Shock Proteins
Humans
Inclusion Bodies
Keratin-14
Leukemia Inhibitory Factor
Proteins
Recombinant Proteins
Tetrahydrofolate Dehydrogenase
Bacteria (microorganisms)
Mammalia
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Packaging protein drugs as bacterial inclusion bodies for therapeutic applications
Article
Text
11
76
openAccess
ORIGINAL
art_10.1186_1475-2859-11-76.pdf
art_10.1186_1475-2859-11-76.pdf
application/pdf
1183320
https://www.repo.uni-hannover.de/bitstream/123456789/660/1/art_10.1186_1475-2859-11-76.pdf
96894b03b2a4b0064c8cb330c90e6410
MD5
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art_10.1186_1475-2859-11-76.pdf.txt
art_10.1186_1475-2859-11-76.pdf.txt
Extracted Text
text/plain
22424
https://www.repo.uni-hannover.de/bitstream/123456789/660/2/art_10.1186_1475-2859-11-76.pdf.txt
411d9cf759f07c0bdc13e4f3b5b31f4e
MD5
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art_10.1186_1475-2859-11-76.pdf.jpg
art_10.1186_1475-2859-11-76.pdf.jpg
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1650
https://www.repo.uni-hannover.de/bitstream/123456789/660/3/art_10.1186_1475-2859-11-76.pdf.jpg
2618eae776cffeb6de9d0aed198a7492
MD5
3
123456789/660
oai:www.repo.uni-hannover.de:123456789/660
2022-12-02 17:14:09.949
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6622022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Vanz, Ana Leticia
414d19cf-c205-4f73-8c5c-f4bdcf0c7a8d
600
Lünsdorf, Heinrich
a88e6531-1491-494f-a573-062cc3018a9b
600
Adnan, Ahmad
071a1922-3f3c-4f05-9131-431e6ce3aadc
600
Nimtz, Manfred
709ca5f1-c9e0-485a-8109-70be748d8c0c
600
Gurramkonda, Chandrasekhar
391a7d35-8a9b-4ea8-a8a4-fe28648d1914
600
Khanna, Navin
325ce125-a27a-4266-82e4-a94def981484
600
Rinas, Ursula
7399be1d-6517-4855-8bdf-d054fa56ebaa
600
2016-11-03T09:29:51Z
2016-11-03T09:29:51Z
2012
Vanz, Ana Leticia; Lünsdorf, H.; Adnan, A.; Nimtz, M.; Gurramkonda, C. Et al.: Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: Catabolic adaptation, stress responses, and autophagic processes. In: Microbial Cell Factories 11 (2012), 103. DOI: http://dx.doi.org/10.1186/1475-2859-11-103
http://www.repo.uni-hannover.de/handle/123456789/662
http://dx.doi.org/10.15488/638
Background: Pichia pastoris is an established eukaryotic host for the production of recombinant proteins. Most often, protein production is under the control of the strong methanol-inducible aox1 promoter. However, detailed information about the physiological alterations in P. pastoris accompanying the shift from growth on glycerol to methanol-induced protein production under industrial relevant conditions is missing. Here, we provide an analysis of the physiological response of P. pastoris GS115 to methanol-induced high-level production of the Hepatitis B virus surface antigen (HBsAg). High product titers and the retention of the protein in the endoplasmic reticulum (ER) are supposedly of major impact on the host physiology. For a more detailed understanding of the cellular response to methanol-induced HBsAg production, the time-dependent changes in the yeast proteome and ultrastructural cell morphology were analyzed during the production process.Results: The shift from growth on glycerol to growth and HBsAg production on methanol was accompanied by a drastic change in the yeast proteome. In particular, enzymes from the methanol dissimilation pathway started to dominate the proteome while enzymes from the methanol assimilation pathway, e.g. the transketolase DAS1, increased only moderately. The majority of methanol was metabolized via the energy generating dissimilatory pathway leading to a corresponding increase in mitochondrial size and numbers. The methanol-metabolism related generation of reactive oxygen species induced a pronounced oxidative stress response (e.g. strong increase of the peroxiredoxin PMP20). Moreover, the accumulation of HBsAg in the ER resulted in the induction of the unfolded protein response (e.g. strong increase of the ER-resident disulfide isomerase, PDI) and the ER associated degradation (ERAD) pathway (e.g. increase of two cytosolic chaperones and members of the AAA ATPase superfamily) indicating that potential degradation of HBsAg could proceed via the ERAD pathway and through the proteasome. However, the amount of HBsAg did not show any significant decline during the cultivation revealing its general protection from proteolytic degradation. During the methanol fed-batch phase, induction of vacuolar proteases (e.g. strong increase of APR1) and constitutive autophagic processes were observed. Vacuolar enclosures were mainly found around peroxisomes and not close to HBsAg deposits and, thus, were most likely provoked by peroxisomal components damaged by reactive oxygen species generated by methanol oxidation.Conclusions: In the methanol fed-batch phase P. pastoris is exposed to dual stress; stress resulting from methanol degradation and stress resulting from the production of the recombinant protein leading to the induction of oxidative stress and unfolded protein response pathways, respectively. Finally, the modest increase of methanol assimilatory enzymes compared to the strong increase of methanol dissimilatory enzymes suggests here a potential to increase methanol incorporation into biomass/product through metabolic enhancement of the methanol assimilatory pathway.
Made available in DSpace on 2016-11-03T09:29:51Z (GMT). No. of bitstreams: 0
Previous issue date: 2012
DBT (India)
BMBF
publishedVersion
eng
London : BioMed Central Ltd.
Microbial Cell Factories 11 (2012)
1475-2859
http://dx.doi.org/10.1186/1475-2859-11-103
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Aox1 promoter
Autophagy
Carbon metabolism
ER stress
Pichia pastoris
Proteome
alcohol oxidase
glycerol
hepatitis B surface antigen
methanol
peroxiredoxin
reactive oxygen metabolite
alcohol metabolism
alcohol oxidation
article
autophagy
catabolism
cell count
cell size
cell structure
cell vacuole
endoplasmic reticulum
endoplasmic reticulum associated degradation
fed batch culture
fungus growth
mitochondrion
nonhuman
oxidative stress
Pichia pastoris
protein degradation
protein unfolding
upregulation
Autophagy
Endoplasmic Reticulum-Associated Degradation
Fungal Proteins
Glycerol
Hepatitis B Surface Antigens
Methanol
Molecular Chaperones
Oxidative Stress
Peroxiredoxins
Pichia
Proteasome Endopeptidase Complex
Protein Disulfide-Isomerases
Reactive Oxygen Species
Recombinant Proteins
Unfolded Protein Response
Vacuoles
Eukaryota
Hepatitis B virus
Pichia pastoris
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: Catabolic adaptation, stress responses, and autophagic processes
Article
Text
11
103
openAccess
ORIGINAL
art_10.1186_1475-2859-11-103.pdf
art_10.1186_1475-2859-11-103.pdf
application/pdf
1497248
https://www.repo.uni-hannover.de/bitstream/123456789/662/1/art_10.1186_1475-2859-11-103.pdf
ec15875c51526281ea6f735139b47e5b
MD5
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art_10.1186_1475-2859-11-103.pdf.txt
art_10.1186_1475-2859-11-103.pdf.txt
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text/plain
50301
https://www.repo.uni-hannover.de/bitstream/123456789/662/2/art_10.1186_1475-2859-11-103.pdf.txt
396ee97b20085d6a0da7383372162f91
MD5
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art_10.1186_1475-2859-11-103.pdf.jpg
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https://www.repo.uni-hannover.de/bitstream/123456789/662/3/art_10.1186_1475-2859-11-103.pdf.jpg
028923e30586645f1429ea34d324ef08
MD5
3
123456789/662
oai:www.repo.uni-hannover.de:123456789/662
2022-12-02 17:14:10.108
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6742022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Schmidt, Simone
d7984148-65d2-402a-b376-ef6ddf6c19ba
600
Willers, Janina
dd7fdfae-5935-422e-82df-892ba07197f7
600
Stahl, Frank
9e2ff2ed-280d-46d6-a902-35bd40adfecd
600
Mutz, Kai-Oliver
382a8adf-6804-4961-abb8-027283d3ca38
600
Scheper, Thomas
05a38e4d-93b1-43ab-8ffc-43e89bb8a642
600
Hahn, Andreas
83433526-0762-4a50-8440-488dcf43c9cc
600
Schuchardt, Jan Philipp
17ca4573-3c79-435c-9144-a8559917c3c0
600
2016-11-03T12:40:19Z
2016-11-03T12:40:19Z
2012
Schmidt, Simone; Willers, Janina; Stahl, Frank; Mutz, Kai-Oliver; Scheper, Thomas et al.: Regulation of lipid metabolism-related gene expression in whole blood cells of normo- and dyslipidemic men after fish oil supplementation. In: Lipids in Health and Disease 11 (2012), 172. DOI: http://dx.doi.org/10.1186/1476-511X-11-172
http://www.repo.uni-hannover.de/handle/123456789/674
http://dx.doi.org/10.15488/650
Background: Beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on the lipid levels of dyslipidemic subjects are widely described in the literature. However, the underlying molecular mechanisms are largely unknown. The aim of this study was to investigate the effects of n-3 PUFAs on the expression of lipid metabolism-related genes in normo- and dyslipidemic men to unveil potential genes and pathways affecting lipid metabolism. Methods. Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with six fish oil capsules per day, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. The gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction. Results: Several transcription factors (peroxisome proliferator-activated receptor α (PPARα), retinoid X receptor (RXR) α, RXRγ, hepatic nuclear factor (HNF) 6, and HNF1ß) as well as other genes related to triacylglycerol (TG) synthesis or high-density lipoprotein (HDL-C) and cholesterol metabolism (phospholipids transfer protein, ATP-binding cassette sub-family G member 5, 2-acylglycerol O-acyltransferase (MOGAT) 3, MOGAT2, diacylglycerol O-acyltransferase 1, sterol O-acyltransferase 1, apolipoprotein CII, and low-density lipoprotein receptor) were regulated after n-3 PUFA supplementation, especially in dyslipidemic men. Conclusion: Gene expression analyses revealed several possible molecular pathways by which n-3 PUFAs lower the TG level and increase the HDL-C and low-density lipoprotein level, whereupon the regulation of PPARα appear to play a central role. Trial registration. ClinicalTrials.gov (ID: NCT01089231).
Made available in DSpace on 2016-11-03T12:40:19Z (GMT). No. of bitstreams: 0
Previous issue date: 2012
BMBF
publishedVersion
eng
London : BioMed Central Ltd.
Lipids in Health and Disease 11 (2012)
1476-511X
http://dx.doi.org/10.1186/1476-511X-11-172
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Dyslipidemia
HNF
Omega-3 fatty acids
PPARα
RXR
TG lowering
2 acylglycerol o acyltransferase 2
2 acylglycerol o acyltransferase 3
ABC transporter G5
acyltransferase
apolipoprotein C2
cholesterol acyltransferase
cholesterol acyltransferase 1
diacylglycerol acyltransferase 1
docosahexaenoic acid
fish oil
hepatic nuclear factor 1beta
hepatic nuclear factor 6
high density lipoprotein cholesterol
icosapentaenoic acid
low density lipoprotein receptor
membrane protein
nuclear factor
omega 3 fatty acid
peroxisome proliferator activated receptor alpha
phospholipid transfer protein
retinoid X receptor alpha
retinoid X receptor gamma
triacylglycerol
unclassified drug
article
blood cell
blood sampling
cholesterol metabolism
clinical article
controlled clinical trial
controlled study
diet supplementation
dyslipidemia
gene expression
genome analysis
human
lipid blood level
lipid metabolism
lipogenesis
male
microarray analysis
nucleotide sequence
protein blood level
real time polymerase chain reaction
Adult
Cholesterol, HDL
Dietary Supplements
Dyslipidemias
Fatty Acids, Omega-3
Fish Oils
Gene Expression
Genome, Human
Humans
Lipid Metabolism
Lipids
Lipoproteins, LDL
Male
Microarray Analysis
Middle Aged
PPAR alpha
Triglycerides
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Regulation of lipid metabolism-related gene expression in whole blood cells of normo- and dyslipidemic men after fish oil supplementation
Article
Text
11
172
openAccess
ORIGINAL
art_10.1186_1476-511X-11-172.pdf
art_10.1186_1476-511X-11-172.pdf
application/pdf
624213
https://www.repo.uni-hannover.de/bitstream/123456789/674/1/art_10.1186_1476-511X-11-172.pdf
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MD5
1
TEXT
art_10.1186_1476-511X-11-172.pdf.txt
art_10.1186_1476-511X-11-172.pdf.txt
Extracted Text
text/plain
52368
https://www.repo.uni-hannover.de/bitstream/123456789/674/2/art_10.1186_1476-511X-11-172.pdf.txt
f206edfaad330bfbed7813deb23bde49
MD5
2
THUMBNAIL
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art_10.1186_1476-511X-11-172.pdf.jpg
Generated Thumbnail
image/jpeg
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https://www.repo.uni-hannover.de/bitstream/123456789/674/3/art_10.1186_1476-511X-11-172.pdf.jpg
9367356a457ffc2c2e37be28afd45ec6
MD5
3
123456789/674
oai:www.repo.uni-hannover.de:123456789/674
2022-12-02 17:14:10.058
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6772022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Chatterjee, Debanjana
65fed7c3-f1d5-4001-ad38-ab54322286a3
600
Marquardt, Nicole
c3a295a5-10ca-4a38-9450-d590fb942755
600
Tufa, Dejene Milkessa
21d47194-0bbb-4122-99e4-6242e8f99210
600
Beauclair, Guillaume
a796512d-f200-45d7-bf7f-a931667abcc0
600
Low, Hui Zhi
2a8fbb40-19e8-4ca9-a334-dd796d8a6679
600
Hatlapatka, Tim
6a6ac788-aad0-415b-b0fb-f502949747c0
600
Hass, Ralf
8dfe1a2a-e5d4-48c2-b7fc-97ef08dfedc7
600
Kasper, Cornelia
8b273750-8fa6-4393-9c1e-b3d7ff3bddee
600
Kaisenberg, Constantin von
2cf3fec4-3db3-4679-aafe-1ddc045c53d0
600
Schmidt, Reinhold Ernst
0a63095d-f9c4-4351-bc7f-3037f4f83570
600
Jacobs, Roland
145d77fa-1144-4ff9-956b-2eb356e7017e
600
2016-11-03T12:40:19Z
2016-11-03T12:40:19Z
2014
Chatterjee, D.; Marquardt, N.; Tufa, D.M.; Beauclair, G.; Low, H.Z. et al.: Role of gamma-secretase in human umbilical-cord derived mesenchymal stem cell mediated suppression of NK cell cytotoxicity. In: Cell Communication and Signaling 12 (2014), Nr. 1, 63. DOI: http://dx.doi.org/10.1186/s12964-014-0063-9
http://www.repo.uni-hannover.de/handle/123456789/677
http://dx.doi.org/10.15488/653
Background: Mesenchymal stem cells (MSCs) are increasingly considered to be used as biological immunosuppressants in hematopoietic stem cell transplantation (HSCT). In the early reconstitution phase following HSCT, natural killer (NK) cells represent the major lymphocyte population in peripheral blood and display graft-vs-leukemia (GvL) effects. The functional interactions between NK cells and MSCs have the potential to influence the leukemia relapse rate after HSCT. Until date, MSC-NK cell interaction studies are largely focussed on bone marrow derived (BM)-MSCs. Umbilical cord derived (UC)-MSCs might be an alternative source of therapeutic MSCs. Thus, we studied the interaction of UC-MSCs with unstimulated allogeneic NK cells.Results: UC-MSCs could potently suppress NK cell cytotoxicity in overnight cultures via soluble factors. The main soluble immunosuppressant was identified as prostaglandin (PG)-E2. Maximal PGE2 release involved IL-1β priming of MSCs after close contact between the NK cells and UC-MSCs. Interestingly, blocking gamma-secretase activation alleviated the immunosuppression by controlling PGE2 production. IL-1 receptor activation and subsequent downstream signalling events were found to require gamma-secretase activity.Conclusion: Although the role of PGE2 in NK cell-MSC has been reported, the requirement of cell-cell contact for PGE2 induced immunosuppression remained unexplained. Our findings shed light on this puzzling observation and identify new players in the NK cell-MSC crosstalk.
Made available in DSpace on 2016-11-03T12:40:19Z (GMT). No. of bitstreams: 0
Previous issue date: 2014
DFG/SFB738/A5
Hannover Biomedical Research School (HBRS)
REBIRTH Cluster of Excellence
Niedersächsische Krebsgesellschaft e.V.
publishedVersion
eng
London : BioMed Central Ltd.
Cell Communication and Signaling 12 (2014), Nr. 1
1478-811X
http://dx.doi.org/10.1186/s12964-014-0063-9
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
Gamma-secretase
IL-1β
Immunosuppression
NK cell cytotoxicity
UC-MSC
5' nucleotidase
CD14 antigen
CD16 antigen
CD19 antigen
CD27 antigen
CD45 antigen
CD69 antigen
CD94 antigen
cyclooxygenase 2
endoglin
gamma secretase
HLA DR antigen
interleukin 1 receptor
interleukin 1beta
Janus kinase
killer cell immunoglobulin like receptor
kynurenine
L selectin
lysosome associated membrane protein 1
natural killer cell receptor NKG2A
natural killer cell receptor NKG2D
presenilin
prostaglandin E2
Thy 1 antigen
antigen expression
Article
blood sampling
cell culture
cell differentiation
cell function
cell interaction
coculture
controlled study
degranulation
enzyme activation
enzyme activity
enzyme linked immunosorbent assay
enzyme phosphorylation
flow cytometry
human
human cell
immunostimulation
mesenchymal stem cell
molecular interaction
natural killer cell mediated cytotoxicity
normal human
peripheral blood mononuclear cell
phenotype
prostaglandin release
protein degradation
protein function
signal transduction
umbilical cord
upregulation
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Role of gamma-secretase in human umbilical-cord derived mesenchymal stem cell mediated suppression of NK cell cytotoxicity
Article
Text
1
12
63
openAccess
ORIGINAL
art_10.1186_s12964-014-0063-9.pdf
art_10.1186_s12964-014-0063-9.pdf
application/pdf
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https://www.repo.uni-hannover.de/bitstream/123456789/677/1/art_10.1186_s12964-014-0063-9.pdf
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MD5
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art_10.1186_s12964-014-0063-9.pdf.txt
Extracted Text
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48570
https://www.repo.uni-hannover.de/bitstream/123456789/677/2/art_10.1186_s12964-014-0063-9.pdf.txt
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MD5
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MD5
3
123456789/677
oai:www.repo.uni-hannover.de:123456789/677
2022-12-02 17:14:10.304
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6802022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Martínez-Alonso, Monica
e65168fa-91f7-4071-adb9-ed675025cd7a
600
García-Fruitós, Elena
8d581785-169f-42b8-ae92-7bfcee3f5d8b
600
Ferrer-Miralles, Neus
c971e229-2aed-4392-998a-4b4586f6d829
600
Rinas, Ursula
7399be1d-6517-4855-8bdf-d054fa56ebaa
600
Villaverde, Antonio
dff5add0-c2f6-46e3-9653-c92d80ca3eab
600
2016-11-04T10:43:01Z
2016-11-04T10:43:01Z
2010
Martínez-Alonso, M.; García-Fruitós, E.; Ferrer-Miralles, N.; Rinas, Ursula; Villaverde, A.: Side effects of chaperone gene co-expression in recombinant protein production. In: Microbial Cell Factories 9 (2010), 64. DOI: http://dx.doi.org/10.1186/1475-2859-9-64
http://www.repo.uni-hannover.de/handle/123456789/680
http://dx.doi.org/10.15488/656
Insufficient availability of molecular chaperones is observed as a major bottleneck for proper protein folding in recombinant protein production. Therefore, co-production of selected sets of cell chaperones along with foreign polypeptides is a common approach to increase the yield of properly folded, recombinant proteins in bacterial cell factories. However, unbalanced amounts of folding modulators handling folding-reluctant protein species might instead trigger undesired proteolytic activities, detrimental regarding recombinant protein stability, quality and yield. This minireview summarizes the most recent observations of chaperone-linked negative side effects, mostly focusing on DnaK and GroEL sets, when using these proteins as folding assistant agents. These events are discussed in the context of the complexity of the cell quality network and the consequent intricacy of the physiological responses triggered by protein misfolding.
Made available in DSpace on 2016-11-04T10:43:01Z (GMT). No. of bitstreams: 0
Previous issue date: 2010
MEC/BIO2007-61194
The Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN, Spain)
European Regional Development Fund
publishedVersion
eng
London : BioMed Central Ltd.
Microbial Cell Factories 9 (2010)
1475-2859
http://dx.doi.org/10.1186/1475-2859-9-64
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
chaperone
chaperonin
Escherichia coli protein
protein ClpB
protein DnaJ
protein DnaK
recombinant protein
bacterial protein
chaperone
chaperonin
dnaK protein, E coli
Escherichia coli protein
heat shock protein 70
article
bacterial cell
cell inclusion
cell transformation
conformational transition
gene expression
growth inhibition
nonhuman
physiological process
process development
process optimization
protein aggregation
protein analysis
protein binding
protein conformation
protein degradation
protein engineering
protein folding
protein function
protein protein interaction
protein quality
protein secretion
protein synthesis
protein targeting
quality control
solubility
biosynthesis
chemistry
genetics
metabolism
protein stability
review
Bacteria (microorganisms)
Bacterial Proteins
Chaperonin 60
Escherichia coli Proteins
HSP70 Heat-Shock Proteins
Molecular Chaperones
Protein Folding
Protein Stability
Recombinant Proteins
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Side effects of chaperone gene co-expression in recombinant protein production
Article
Text
9
64
openAccess
ORIGINAL
art_10.1186_1475-2859-9-64.pdf
art_10.1186_1475-2859-9-64.pdf
application/pdf
577567
https://www.repo.uni-hannover.de/bitstream/123456789/680/1/art_10.1186_1475-2859-9-64.pdf
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MD5
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art_10.1186_1475-2859-9-64.pdf.txt
art_10.1186_1475-2859-9-64.pdf.txt
Extracted Text
text/plain
33589
https://www.repo.uni-hannover.de/bitstream/123456789/680/2/art_10.1186_1475-2859-9-64.pdf.txt
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MD5
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MD5
3
123456789/680
oai:www.repo.uni-hannover.de:123456789/680
2022-12-02 17:14:10.516
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6822022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Li, Zhaopeng
4c747e83-b3fa-429d-8a3d-71f0bc18a215
600
Carstensen, Bettina
e0882bb7-1621-42c8-b80a-d75a1efa08b5
600
Rinas, Ursula
7399be1d-6517-4855-8bdf-d054fa56ebaa
600
2016-11-04T10:43:02Z
2016-11-04T10:43:02Z
2014
Li, Zhaopeng; Carstensen, Bettina; Rinas, Ursula: Smart sustainable bottle (SSB) system for E. coli based recombinant protein production. In: Microbial Cell Factories 13 (2014), Nr. 1, 153. DOI: http://dx.doi.org/10.1186/s12934-014-0153-9
http://www.repo.uni-hannover.de/handle/123456789/682
http://dx.doi.org/10.15488/658
Background: Recombinant proteins are usually required in laboratories interested in the protein but not in the production process itself. Thus, technical equipment which is easy to handle and straight forward protein production procedures are of great benefit to those laboratories. Companies selling single use cultivation bags and bioreactors are trying to satisfy at least part of these needs. However, single-use systems can contribute to major costs which might be acceptable when "good manufacturing practices" are required but not acceptable for most laboratories facing tight funding. Results: The assembly and application of a simple self-made "smart sustainable bottle" (SSB) system for E. coli based protein production is presented. The core of the SSB system is a 2-L glass bottle which is operated at constant temperature, air flow, and stirrer speed without measurement and control of pH and dissolved oxygen. Oxygen transfer capacities are in the range as in conventional bioreactors operated at intermediate aeration rates and by far exceed those found in conventional shaking flasks and disposable bioreactors. The SSB system was applied for the production of various recombinant proteins using T7-based expression systems and a defined autoinduction medium. The production performance regarding amount and solubility of proteins with robust and delicate properties was as good as in state-of-the-art stirred tank commercial bioreactors. Conclusions: The SSB system represents a low cost protein production device applicable for easy, effective, and reproducible recombinant protein production.
Made available in DSpace on 2016-11-04T10:43:02Z (GMT). No. of bitstreams: 0
Previous issue date: 2014
DFG/REBIRTH
publishedVersion
eng
London : BioMed Central Ltd.
Microbial Cell Factories 13 (2014), Nr. 1
1475-2859
http://dx.doi.org/10.1186/s12934-014-0153-9
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
Autoinduction
Escherichia coli
kLa
Oxygen transfer
Self-made bioreactor
recombinant protein
bioreactor
biosynthesis
culture technique
devices
Escherichia coli
gene expression
growth, development and aging
procedures
Bioreactors
Cell Culture Techniques
Escherichia coli
Gene Expression
Recombinant Proteins
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Smart sustainable bottle (SSB) system for E. coli based recombinant protein production
Article
Text
1
13
153
openAccess
ORIGINAL
art_10.1186_s12934-014-0153-9.pdf
art_10.1186_s12934-014-0153-9.pdf
application/pdf
1026505
https://www.repo.uni-hannover.de/bitstream/123456789/682/1/art_10.1186_s12934-014-0153-9.pdf
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MD5
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art_10.1186_s12934-014-0153-9.pdf.txt
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https://www.repo.uni-hannover.de/bitstream/123456789/682/2/art_10.1186_s12934-014-0153-9.pdf.txt
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MD5
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MD5
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123456789/682
oai:www.repo.uni-hannover.de:123456789/682
2022-12-02 17:14:10.317
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6872022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Rueda, Fabian
85bbdd16-9aa0-488a-89f0-aaa37c5abb2f
600
Céspedes, Maria Virtudes
5cf38135-77e1-4bc1-8c6f-8c70e2518e6e
600
Sánchez-Chardi, Alejandro
19417ff4-2638-40c2-a501-8aad24e1626e
600
Seras-Franzoso, Joaquin
56e62755-5c73-416e-8a6c-e5f70a0e8281
600
Pesarrodona, Mireia
71a8823c-bb4b-426c-9f3f-405f4c2590f4
600
Ferrer-Miralles, Neus
c971e229-2aed-4392-998a-4b4586f6d829
600
Vázquez, Esther
aff29e5b-df7c-4785-a355-456dd7fbfe56
600
Rinas, Ursula
7399be1d-6517-4855-8bdf-d054fa56ebaa
600
Unzueta, Ugutz
c54c8a1c-a627-48e8-9ed8-5c29eaf0acd6
600
Mamat, Uwe
83bb40bf-2235-4a6d-ad46-4ff282b5e843
600
Mangues, Ramon
00bb6a94-907f-40b4-940e-19b90c8be635
600
García-Fruitós, Elena
8d581785-169f-42b8-ae92-7bfcee3f5d8b
600
Villaverde, Antonio
dff5add0-c2f6-46e3-9653-c92d80ca3eab
600
2016-11-04T10:43:07Z
2016-11-04T10:43:07Z
2016
Rueda, F.; Céspedes, M.V.; Sánchez-Chardi, A.; Seras-Franzoso, J.; Pesarrodona, M. Et al.: Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli. In: Microbial Cell Factories 15 (2016), Nr. 1, 59. DOI: http://dx.doi.org/10.1186/s12934-016-0457-z
http://www.repo.uni-hannover.de/handle/123456789/687
http://dx.doi.org/10.15488/663
Background: Production of recombinant drugs in process-friendly endotoxin-free bacterial factories targets to a lessened complexity of the purification process combined with minimized biological hazards during product application. The development of nanostructured recombinant materials in innovative nanomedical activities expands such a need beyond plain functional polypeptides to complex protein assemblies. While Escherichia coli has been recently modified for the production of endotoxin-free proteins, no data has been so far recorded regarding how the system performs in the fabrication of smart nanostructured materials. Results: We have here explored the nanoarchitecture and in vitro and in vivo functionalities of CXCR4-targeted, self-assembling protein nanoparticles intended for intracellular delivery of drugs and imaging agents in colorectal cancer. Interestingly, endotoxin-free materials exhibit a distinguishable architecture and altered size and target cell penetrability than counterparts produced in conventional E. coli strains. These variant nanoparticles show an eventual proper biodistribution and highly specific and exclusive accumulation in tumor upon administration in colorectal cancer mice models, indicating a convenient display and function of the tumor homing peptides and high particle stability under physiological conditions. Discussion: The observations made here support the emerging endotoxin-free E. coli system as a robust protein material producer but are also indicative of a particular conformational status and organization of either building blocks or oligomers. This appears to be promoted by multifactorial stress-inducing conditions upon engineering of the E. coli cell envelope, which impacts on the protein quality control of the cell factory.
Made available in DSpace on 2016-11-04T10:43:07Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
publishedVersion
eng
London : BioMed Central Ltd.
Microbial Cell Factories 15 (2016), Nr. 1
1475-2859
http://dx.doi.org/10.1186/s12934-016-0457-z
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
Biodistribution
Biomaterials
E. coli
Endotoxin-free strains
Nanomedicine
Nanoparticles
Protein engineering
Recombinant proteins
chemokine receptor CXCR4
endotoxin
nanoparticle
animal experiment
animal model
animal tissue
Article
bacterial strain
bioaccumulation
colorectal cancer
controlled study
Escherichia coli
in vitro study
in vivo study
mouse
nanofabrication
nonhuman
protein assembly
protein engineering
protein structure
quality control
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli
Article
Text
1
15
59
openAccess
ORIGINAL
art_10.1186_s12934-016-0457-z.pdf
art_10.1186_s12934-016-0457-z.pdf
application/pdf
3155595
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MD5
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MD5
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MD5
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123456789/687
oai:www.repo.uni-hannover.de:123456789/687
2022-12-02 17:14:10.313
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6892022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Henne, Melina
9baad0ad-74bd-4064-b218-e4c7c5e41360
600
König, Nicolas
523a296d-6308-4a69-961a-116377a644ef
600
Triulzi, Tiziana
9200dea0-5040-4e44-8351-1f12828fd55f
600
Baroni, Sara
53763303-d356-4688-8355-246138d8aed0
600
Forlani, Fabio
9f30345a-3a0c-4a4f-ae1e-6123b9822d37
600
Scheibe, Renate
8acacb0a-b117-461a-bc6b-d87b7e550bd4
600
Papenbrock, Jutta
5c949a38-3b52-4d67-9745-9ed13b0a68ed
600
2016-11-04T10:43:07Z
2016-11-04T10:43:07Z
2015
Henne, Melina; König, N.; Triulzi, Tiziana; Baroni, S.; Forlani, F. et al.: Sulfurtransferase and thioredoxin specifically interact as demonstrated by bimolecular fluorescence complementation analysis and biochemical tests. In: FEBS Open Bio 5 (2015), S. 832-843. DOI: http://dx.doi.org/10.1016/j.fob.2015.10.001
http://www.repo.uni-hannover.de/handle/123456789/689
http://dx.doi.org/10.15488/665
Sulfurtransferases (Strs) and thioredoxins (Trxs) are members of large protein families. Trxs are disulfide reductases and play an important role in redox-related cellular processes. They interact with a broad range of proteins. Strs catalyze the transfer of a sulfur atom from a suitable sulfur donor to nucleophilic sulfur acceptors in vitro, but the physiological roles of these enzymes are not well defined. Several studies in different organisms demonstrate protein-protein interactions of Strs with members of the Trx family. We are interested in investigating the specificity of the interaction between Str and Trx isoforms. In order to use the bimolecular fluorescence complementation (BiFC), several Str and Trx sequences from Arabidopsis thaliana were cloned into the pUC-SPYNE and pUC-SPYCE split-YFP vectors, respectively. Each couple of plasmids containing the sequences for the putative interaction partners were transformed into Arabidopsis protoplasts and screened using a confocal laser scanning microscope. Compartment- and partner-specific interactions could be observed in transformed protoplasts. Replacement of cysteine residues in the redox-active site of Trxs abolished the interaction signal. Therefore, the redox site is not only involved in the redox reaction but also responsible for the interaction with partner proteins. Biochemical assays support a specific interaction among Strs and certain Trxs. Based on the results obtained, the interaction of Strs and Trxs indicates a role of Strs in the maintenance of the cellular redox homeostasis.
Made available in DSpace on 2016-11-04T10:43:07Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
DFG/PA764/1-4
DFG/PA764/1-5
Università degli Studi di Milano
DAAD
publishedVersion
eng
Amsterdam : Elsevier
FEBS Open Bio 5 (2015)
2211-5463
http://dx.doi.org/10.1016/j.fob.2015.10.001
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
Arabidopsis thaliana
Bimolecular fluorescence complementation
Protein interaction
Thioredoxin
cysteine
recombinant protein
sulfurtransferase
thioredoxin
amino acid sequence
amino acid substitution
Arabidopsis
Arabidopsis thaliana
Article
bimolecular fluorescence complementation
binding site
biochemical analysis
confocal laser microscopy
controlled study
cytoplasm
enzyme activity
fluorescence analysis
in vitro study
molecular weight
nonhuman
oxidation reduction potential
precipitation
priority journal
protein cross linking
protein expression
protein protein interaction
protein purification
protoplast
stoichiometry
substrate concentration
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Sulfurtransferase and thioredoxin specifically interact as demonstrated by bimolecular fluorescence complementation analysis and biochemical tests
Article
Text
5
832
843
openAccess
ORIGINAL
Henne_et_al-2015-FEBS_Open_Bio.pdf
Henne_et_al-2015-FEBS_Open_Bio.pdf
application/pdf
1612886
https://www.repo.uni-hannover.de/bitstream/123456789/689/1/Henne_et_al-2015-FEBS_Open_Bio.pdf
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MD5
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Henne_et_al-2015-FEBS_Open_Bio.pdf.txt
Henne_et_al-2015-FEBS_Open_Bio.pdf.txt
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text/plain
61734
https://www.repo.uni-hannover.de/bitstream/123456789/689/2/Henne_et_al-2015-FEBS_Open_Bio.pdf.txt
d0d1c46dcd7c656e8d7c6fc9f7c36ddf
MD5
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Henne_et_al-2015-FEBS_Open_Bio.pdf.jpg
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MD5
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123456789/689
oai:www.repo.uni-hannover.de:123456789/689
2022-12-02 17:14:10.689
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6952022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Bowman, Andrew
4e82653c-d7c8-4d07-b23f-bf4e265bc5de
600
Lercher, Lukas
974e128d-bd64-4f2c-bc7e-1d217cf88c01
600
Singh, Hari R.
f704b4fe-5ec5-4d3f-9143-b6ea83e2dbe8
600
Zinne, Daria
3e3c3cae-0286-4cca-8bbe-9f4c87b037a6
600
Timinszky, Gyula
0886b593-6780-413b-8a7a-60af11c0b183
600
Carlomagno, Teresa
7265c713-64ff-46a6-bf0a-e461ea0e87d8
600
Ladurner, Andreas G.
4878b2ea-9cd7-4f2e-ba65-b8ac34b16914
600
2016-11-09T08:40:17Z
2016-11-09T08:40:17Z
2015
Bowman, A.; Lercher, Lukas; Singh, H.R.; Zinne, D.; Timinszky, G. et al.: The histone chaperone sNASP binds a conserved peptide motif within the globular core of histone H3 through its TPR repeats. In: Nucleic Acids Research 44 (2015), Nr. 7, S. 3105-3117. DOI: http://dx.doi.org/10.1093/nar/gkv1372
http://www.repo.uni-hannover.de/handle/123456789/695
http://dx.doi.org/10.15488/671
Eukaryotic chromatin is a complex yet dynamic structure, which is regulated in part by the assembly and disassembly of nucleosomes. Key to this process is a group of proteins termed histone chaperones that guide the thermodynamic assembly of nucleosomes by interacting with soluble histones. Here we investigate the interaction between the histone chaperone sNASP and its histone H3 substrate. We find that sNASP binds with nanomolar affinity to a conserved heptapeptide motif in the globular domain of H3, close to the C-terminus. Through functional analysis of sNASP homologues we identified point mutations in surface residues within the TPR domain of sNASP that disrupt H3 peptide interaction, but do not completely disrupt binding to full length H3 in cells, suggesting that sNASP interacts with H3 through additional contacts. Furthermore, chemical shift perturbations from 1H-15N HSQC experiments show that H3 peptide binding maps to the helical groove formed by the stacked TPR motifs of sNASP. Our findings reveal a new mode of interaction between a TPR repeat domain and an evolutionarily conserved peptide motif found in canonical H3 and in all histone H3 variants, including CenpA and have implications for the mechanism of histone chaperoning within the cell.
Made available in DSpace on 2016-11-09T08:40:17Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
Ludwig-Maximilians-Universität München
DFG/SFB/646
DFG/SFB/1064
DFG/CIPSM
DFG/SyNergy
Bavarian BioSysNet programme
European Molecular Biology Laboratory
DFG/TI 817/2-1
Worldwide Cancer Research/14-1315
EMBO Long-term Fellowship
Marie Curie Intra-European Fellowship
publishedVersion
eng
Oxford : Oxford University Press
Nucleic Acids Research 44 (2015), Nr. 7
1362-4962
0305-1048
http://dx.doi.org/10.1093/nar/gkv1372
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
Eukaryotic chromatin
proteins
sNASP
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
The histone chaperone sNASP binds a conserved peptide motif within the globular core of histone H3 through its TPR repeats
Article
Text
7
44
3105
3117
openAccess
ORIGINAL
Nucl. Acids Res.-2016-Bowman-3105-17.pdf
Nucl. Acids Res.-2016-Bowman-3105-17.pdf
application/pdf
6337420
https://www.repo.uni-hannover.de/bitstream/123456789/695/1/Nucl.%20Acids%20Res.-2016-Bowman-3105-17.pdf
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MD5
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TEXT
Nucl. Acids Res.-2016-Bowman-3105-17.pdf.txt
Nucl. Acids Res.-2016-Bowman-3105-17.pdf.txt
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text/plain
64072
https://www.repo.uni-hannover.de/bitstream/123456789/695/2/Nucl.%20Acids%20Res.-2016-Bowman-3105-17.pdf.txt
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MD5
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Nucl. Acids Res.-2016-Bowman-3105-17.pdf.jpg
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MD5
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123456789/695
oai:www.repo.uni-hannover.de:123456789/695
2022-12-02 17:14:10.433
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6962022-12-02T15:06:09Zcom_123456789_1col_123456789_6doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570ddc:600
Bracht, Katja
d843a9ee-a950-4254-b9ef-c1b1579fe58a
600
Angrisani, Nina
ccea4e82-63f4-449e-b0c1-9b8b032891ee
600
Seitz, Jan-Marten
7aa9a78a-0cd5-43f4-84df-c2b5eb91c353
600
Eifler, Rainer
00c8d3d1-63dd-4aca-85dc-15121bfb7bf9
600
Weizbauer, Andreas
e48e0720-8bfb-4054-9d9f-fadd951986de
600
Reifenrath, Janin
4cd37190-194b-46f4-a59f-f6121ea7e075
600
2016-11-09T08:40:17Z
2016-11-09T08:40:17Z
2015
Bracht, K.; Angrisani, N.; Seitz, J.-M.; Eifler, Rainer; Weizbauer, A. Et al.: The influence of storage and heat treatment on a magnesium-based implant material: An in vitro and in vivo study. In: BioMedical Engineering Online 14 (2015), Nr. 1 , 92. DOI: https://doi.org/10.1186/s12938-015-0091-8
http://www.repo.uni-hannover.de/handle/123456789/696
http://dx.doi.org/10.15488/672
Background: Magnesium alloys are recommended as a potential material for osteosynthesis. It is known that storage-induced property modifications can occur in materials like aluminum. Thus the aim of this study was to analyze the influence of storage durations of up to 48weeks on the biomechanical, structural, and degradation properties of the degradable magnesium alloy LAE442. Methods: Extruded implants (n=104; Ø 2.5mm×25mm) were investigated after storage periods of 0, 12, 24, and 48weeks in three different sub-studies: (I) immediately after the respective storage duration and after an additional (II) 56days of in vitro corrosion in simulated body fluid (SFB), and (III) 48weeks in vivo corrosion in a rabbit model, respectively. In addition, the influence of a T5-heat treatment (206°C for 15h in an argon atmosphere) was tested (n=26; 0week of storage). Evaluation was performed by three-point bending, scanning electron microscopy, radiography, μ-computed tomography, evaluation of the mean grain size, and contrast analysis of precipitations (such as aluminum or lithium). Results: The heat treatment induced a significant reduction in initial stability, and enhanced the corrosion resistance. In vivo experiments showed a good biocompatibility for all implants. During the storage of up to 48weeks, no significant changes occurred in the implant properties. Conclusions: LAE442 implants can be safely used after up to 48weeks of storage.
Made available in DSpace on 2016-11-09T08:40:17Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
DFG/SFB/599
publishedVersion
eng
London : BioMed Central Ltd.
BioMedical Engineering Online 14 (2015), Nr. 1
1475-925X
https://doi.org/10.1186/s12938-015-0091-8
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
Degradable
Aluminum
In vitro
In vivo
Magnesium
Storage
Biocompatibility
Computerized tomography
Corrosion
Corrosion resistance
Energy storage
Heat resistance
Heat treatment
Magnesium alloys
Scanning electron microscopy
Degradable
In-vitro corrosions
In-vivo experiments
Initial stabilities
Simulated body fluids
body fluid
Three point bending
Storage (materials)
alloy
lithium
unclassified drug
animal experiment
animal model
biocompatibility
biomechanics
bone mass
controlled study
corrosion
degradation
energy dispersive spectroscopy
evaluation study
ex vivo study
experimental design
grain
heat treatment
implant
in vitro study
in vivo study
micro-computed tomography
nonhuman
precipitation
priority journal
rabbit model
radiography
scanning electron microscopy
spectroscopy
three point bending
Dewey Decimal Classification::600 | Technik
600
600
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
The influence of storage and heat treatment on a magnesium-based implant material: An in vitro and in vivo study
Article
Text
1
14
92
openAccess
ORIGINAL
art_10.1186_s12938-015-0091-8.pdf
art_10.1186_s12938-015-0091-8.pdf
application/pdf
2723131
https://www.repo.uni-hannover.de/bitstream/123456789/696/1/art_10.1186_s12938-015-0091-8.pdf
4f25665656c5c68e3d7e9b2e185a8fb8
MD5
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art_10.1186_s12938-015-0091-8.pdf.txt
art_10.1186_s12938-015-0091-8.pdf.txt
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text/plain
47040
https://www.repo.uni-hannover.de/bitstream/123456789/696/2/art_10.1186_s12938-015-0091-8.pdf.txt
48e04c09b63c24ace2d119ec6bfe1263
MD5
2
THUMBNAIL
art_10.1186_s12938-015-0091-8.pdf.jpg
art_10.1186_s12938-015-0091-8.pdf.jpg
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1730
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MD5
3
123456789/696
oai:www.repo.uni-hannover.de:123456789/696
2022-12-02 16:06:09.434
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/6972022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Li, Zhaopeng
4c747e83-b3fa-429d-8a3d-71f0bc18a215
600
Nimtz, Manfred
709ca5f1-c9e0-485a-8109-70be748d8c0c
600
Rinas, Ursula
7399be1d-6517-4855-8bdf-d054fa56ebaa
600
2016-11-09T08:40:17Z
2016-11-09T08:40:17Z
2014
Li, Zhaopeng; Nimtz, M.; Rinas, Ursula: The metabolic potential of Escherichia coli BL21 in defined and rich medium. In: Microbial Cell Factories 13 (2014), Nr. 1, 45. DOI: http://dx.doi.org/10.1186/1475-2859-13-45
http://www.repo.uni-hannover.de/handle/123456789/697
http://dx.doi.org/10.15488/673
Background: The proteome reflects the available cellular machinery to deal with nutrients and environmental challenges. The most common E. coli strain BL21 growing in different, commonly employed media was evaluated using a detailed quantitative proteome analysis.Results: The presence of preformed biomass precursor molecules in rich media such as Luria Bertani supported rapid growth concomitant to acetate formation and apparently unbalanced abundances of central metabolic pathway enzymes, e.g. high levels of lower glycolytic pathway enzymes as well as pyruvate dehydrogenase, and low levels of TCA cycle and high levels of the acetate forming enzymes Pta and AckA. The proteome of cells growing exponentially in glucose-supplemented mineral salt medium was dominated by enzymes of amino acid synthesis pathways, contained more balanced abundances of central metabolic pathway enzymes, and a lower portion of ribosomal and other translational proteins. Entry into stationary phase led to a reconstruction of the bacterial proteome by increasing e.g. the portion of proteins required for scavenging rare nutrients and general cell protection. This proteomic reconstruction during entry into stationary phase was more noticeable in cells growing in rich medium as they have a greater reservoir of recyclable proteins from the translational machinery.Conclusions: The proteomic comparison of cells growing exponentially in different media reflected the antagonistic and competitive regulation of central metabolic pathways through the global transcriptional regulators Cra, Crp, and ArcA. For example, the proteome of cells growing exponentially in rich medium was consistent with a dominating role of phosphorylated ArcA most likely a result from limitations in reoxidizing reduced quinones in the respiratory chain under these growth conditions. The proteomic alterations of exponentially growing cells into stationary phase cells were consistent with stringent-like and stationary phase responses and a dominating control through DksA-ppGpp and RpoS.
Made available in DSpace on 2016-11-09T08:40:17Z (GMT). No. of bitstreams: 0
Previous issue date: 2014
BMBF/FKZ/0315285D
publishedVersion
eng
London : BioMed Central Ltd.
Microbial Cell Factories 13 (2014), Nr. 1
1475-2859
http://dx.doi.org/10.1186/1475-2859-13-45
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Escherichia coli
pyruvate dehydrogenase
Growth rate control
Metabolic balance
Overflow metabolism
Proteome
Stationary phase response
Transcriptional control
Two-dimensional gel electrophoresis
Pentose Phosphate Pathway
Ketone Oxidoreductases
Glycolysis
Escherichia coli Proteins
Escherichia coli
Energy Metabolism
Electrophoresis, Gel, Two-Dimensional
Culture Media
Citric Acid Cycle
Carbon
Amino Acids
two dimensional gel electrophoresis
transcription regulation
respiratory chain
protein synthesis
protein folding
pyruvate dehydrogenase complex
amino acid synthesis
bacterial growth
bacterial metabolism
bacterial phenomena and functions
carbon metabolism
cell protection
citric acid cycle
culture medium
Escherichia coli
Escherichia coli BL21
glycolysis
metabolic balance
metabolism
nonhuman
nutrient availability
pentose phosphate cycle
protein analysis
protein degradation
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
The metabolic potential of Escherichia coli BL21 in defined and rich medium
Article
Text
1
13
45
openAccess
ORIGINAL
art_10.1186_1475-2859-13-45.pdf
art_10.1186_1475-2859-13-45.pdf
application/pdf
7199783
https://www.repo.uni-hannover.de/bitstream/123456789/697/1/art_10.1186_1475-2859-13-45.pdf
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MD5
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art_10.1186_1475-2859-13-45.pdf.txt
art_10.1186_1475-2859-13-45.pdf.txt
Extracted Text
text/plain
79825
https://www.repo.uni-hannover.de/bitstream/123456789/697/2/art_10.1186_1475-2859-13-45.pdf.txt
ea56904b3bbf20c5e773e4b81c2e38be
MD5
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THUMBNAIL
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art_10.1186_1475-2859-13-45.pdf.jpg
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712bbbe47489f973d9e5e2c79e470cbf
MD5
3
123456789/697
oai:www.repo.uni-hannover.de:123456789/697
2022-12-02 17:14:10.325
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/7012022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Weisman, David
f36af7ac-e82b-4403-b2b5-ba5ca1819eba
-1
Alkio, Merianne
74435a77-48f6-4d02-a189-ece413487b60
-1
Colón-Carmona, Adan
7c7a2669-b8dc-4e59-bb24-b6e81e420201
-1
2016-11-09T10:37:57Z
2016-11-09T10:37:57Z
2010
Weisman, D.; Alkio, Merianne; Colón-Carmona, A.: Transcriptional responses to polycyclic aromatic hydrocarbon-induced stress in Arabidopsis thaliana reveal the involvement of hormone and defense signaling pathways. In: BMC Plant Biology 10 (2010), 59. DOI: http://dx.doi.org/10.1186/1471-2229-10-59
http://www.repo.uni-hannover.de/handle/123456789/701
http://dx.doi.org/10.15488/677
Background: Polycyclic aromatic hydrocarbons (PAHs) are toxic, widely-distributed, environmentally persistent, and carcinogenic byproducts of carbon-based fuel combustion. Previously, plant studies have shown that PAHs induce oxidative stress, reduce growth, and cause leaf deformation as well as tissue necrosis. To understand the transcriptional changes that occur during these processes, we performed microarray experiments on Arabidopsis thaliana L. under phenanthrene treatment, and compared the results to published Arabidopsis microarray data representing a variety of stress and hormone treatments. In addition, to probe hormonal aspects of PAH stress, we assayed transgenic ethylene-inducible reporter plants as well as ethylene pathway mutants under phenanthrene treatment.Results: Microarray results revealed numerous perturbations in signaling and metabolic pathways that regulate reactive oxygen species (ROS) and responses related to pathogen defense. A number of glutathione S-transferases that may tag xenobiotics for transport to the vacuole were upregulated. Comparative microarray analyses indicated that the phenanthrene response was closely related to other ROS conditions, including pathogen defense conditions. The ethylene-inducible transgenic reporters were activated by phenanthrene. Mutant experiments showed that PAH inhibits growth through an ethylene-independent pathway, as PAH-treated ethylene-insensitive etr1-4 mutants exhibited a greater growth reduction than WT. Further, phenanthrene-treated, constitutive ethylene signaling mutants had longer roots than the untreated control plants, indicating that the PAH inhibits parts of the ethylene signaling pathway.Conclusions: This study identified major physiological systems that participate in the PAH-induced stress response in Arabidopsis. At the transcriptional level, the results identify specific gene targets that will be valuable in finding lead compounds and engineering increased tolerance. Collectively, the results open a number of new avenues for researching and improving plant resilience and PAH phytoremediation.
Made available in DSpace on 2016-11-09T10:37:57Z (GMT). No. of bitstreams: 0
Previous issue date: 2010
University of Massachusetts Boston
National Science Foundation/IBN-0343856)
publishedVersion
eng
London : BioMed Central Ltd.
BMC Plant Biology 10 (2010)
1471-2229
http://dx.doi.org/10.1186/1471-2229-10-59
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Arabidopsis
Arabidopsis thaliana
1 aminocyclopropanecarboxylic acid
1-aminocyclopropane-1-carboxylic acid
amino acid
beta glucuronidase
ethylene
ethylene derivative
phenanthrene
phenanthrene derivative
phytohormone
polycyclic aromatic hydrocarbon
Arabidopsis
article
Botrytis
cluster analysis
DNA microarray
drug effect
gene expression profiling
gene expression regulation
genetic database
genetic transcription
genetics
histology
immunology
metabolism
microbiology
mutation
photoperiodicity
physiological stress
plant growth
plant root
reporter gene
signal transduction
transgenic plant
Amino Acids, Cyclic
Arabidopsis
Botrytis
Cluster Analysis
Databases, Genetic
Ethylenes
Gene Expression Profiling
Gene Expression Regulation, Plant
Genes, Reporter
Glucuronidase
Hypocotyl
Mutation
Oligonucleotide Array Sequence Analysis
Phenanthrenes
Photoperiod
Plant Growth Regulators
Plant Roots
Plants, Genetically Modified
Polycyclic Hydrocarbons, Aromatic
Signal Transduction
Stress, Physiological
Transcription, Genetic
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Transcriptional responses to polycyclic aromatic hydrocarbon-induced stress in Arabidopsis thaliana reveal the involvement of hormone and defense signaling pathways
Article
Text
10
59
openAccess
ORIGINAL
art_10.1186_1471-2229-10-59.pdf
art_10.1186_1471-2229-10-59.pdf
application/pdf
2045295
https://www.repo.uni-hannover.de/bitstream/123456789/701/1/art_10.1186_1471-2229-10-59.pdf
0b79792ee31a6225e71f28e8889f6f8d
MD5
1
TEXT
art_10.1186_1471-2229-10-59.pdf.txt
art_10.1186_1471-2229-10-59.pdf.txt
Extracted Text
text/plain
64647
https://www.repo.uni-hannover.de/bitstream/123456789/701/2/art_10.1186_1471-2229-10-59.pdf.txt
ab2d51334d1d688bdc5d744e3b8ed808
MD5
2
THUMBNAIL
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art_10.1186_1471-2229-10-59.pdf.jpg
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1681
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b532f6c072facd38833f48be354ca401
MD5
3
123456789/701
oai:www.repo.uni-hannover.de:123456789/701
2022-12-02 17:14:10.308
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/7022022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570
Schmidt, Simone
d7984148-65d2-402a-b376-ef6ddf6c19ba
600
Stahl, Frank
9e2ff2ed-280d-46d6-a902-35bd40adfecd
600
Mutz, Kai-Oliver
382a8adf-6804-4961-abb8-027283d3ca38
600
Scheper, Thomas
05a38e4d-93b1-43ab-8ffc-43e89bb8a642
600
Hahn, Andreas
83433526-0762-4a50-8440-488dcf43c9cc
600
Schuchardt, Jan Philipp
17ca4573-3c79-435c-9144-a8559917c3c0
600
2016-11-09T10:37:57Z
2016-11-09T10:37:57Z
2012
Schmidt, Simone; Stahl, Frank; Mutz, Kai-Oliver; Scheper, Thomas; Hahn, Andreas et al.: Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men. In: Nutrition and Metabolism 9 (2012), 45. DOI: http://dx.doi.org/10.1186/1743-7075-9-45
http://www.repo.uni-hannover.de/handle/123456789/702
http://dx.doi.org/10.15488/678
Background: The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially in dyslipidemic subjects with a high risk of cardiovascular disease, are widely described in the literature. A lot of effects of n-3 PUFAs and their oxidized metabolites are triggered by regulating the expression of genes. Currently, it is uncertain if the administration of n-3 PUFAs results in different expression changes of genes related to antioxidative mechanisms in normo- and dyslipidemic subjects, which may partly explain their cardioprotective effects. The aim of this study was to investigate the effects of n-3 PUFA supplementation on expression changes of genes involved in oxidative processes. Methods: Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with fish oil capsules, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. Gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction (qRT-PCR). Results: Using microarrays, we discovered an increased expression of antioxidative enzymes and a decreased expression of pro-oxidative and tissue enzymes, such as cytochrome P450 enzymes and matrix metalloproteinases, in both normo- and dyslipidemic men. An up-regulation of catalase and heme oxigenase 2 in both normo- and dyslipidemic subjects and an up-regulation of cytochrome P450 enzyme 1A2 only in dyslipidemic subjects could be observed by qRT-PCR analysis. Conclusions: Supplementation of normo- and dyslipidemic subjects with n-3 PUFAs changed the expression of genes related to oxidative processes, which may suggest antioxidative and potential cardioprotective effects of n-3 PUFAs. Further studies combining genetic and metabolic endpoints are needed to verify the regulative effects of n-3 PUFAs in antioxidative gene expression to better understand their beneficial effects in health and disease prevention.
Made available in DSpace on 2016-11-09T10:37:57Z (GMT). No. of bitstreams: 0
Previous issue date: 2012
BMBF
publishedVersion
eng
London : BioMed Central Ltd.
Nutrition and Metabolism 9 (2012)
1743-7075
http://dx.doi.org/10.1186/1743-7075-9-45
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
Antioxidative defence
Catalase
Cytochrome P450 enzyme
Dyslipidemia
Glutathione
Heme oxygenase
Matrix metalloproteinase
Omega-3 fatty acids
Oxylipines
catalase
cytochrome P450
cytochrome P450 1A2
docosahexaenoic acid
gelatinase A
glutathione reductase
glutathione transferase
heme oxygenase 2
icosapentaenoic acid
immunoglobulin enhancer binding protein
matrix metalloproteinase
matrix metalloproteinase 25
mitogen activated protein kinase
omega 3 fatty acid
stromelysin
superoxide dismutase
transcriptome
unclassified drug
adult
antioxidant activity
article
clinical article
controlled study
down regulation
drug effect
dyslipidemia
erythrocyte membrane
fatty acid analysis
gene control
gene expression
genetic transcription
heart protection
human
male
microarray analysis
nucleotide sequence
oxidative stress
randomized controlled trial
real time polymerase chain reaction
upregulation
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men
Article
Text
9
45
openAccess
ORIGINAL
art_10.1186_1743-7075-9-45.pdf
art_10.1186_1743-7075-9-45.pdf
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MD5
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123456789/702
oai:www.repo.uni-hannover.de:123456789/702
2022-12-02 17:14:10.321
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/7072022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Lünsdorf, Heinrich
a88e6531-1491-494f-a573-062cc3018a9b
600
Gurramkonda, Chandrasekhar
391a7d35-8a9b-4ea8-a8a4-fe28648d1914
600
Adnan, Ahmad
071a1922-3f3c-4f05-9131-431e6ce3aadc
600
Khanna, Navin
325ce125-a27a-4266-82e4-a94def981484
600
Rinas, Ursula
7399be1d-6517-4855-8bdf-d054fa56ebaa
600
2016-11-09T10:37:58Z
2016-11-09T10:37:58Z
2011
Lünsdorf, H.; Gurramkonda, C.; Adnan, A.; Khanna, N.; Rinas, Ursula: Virus-like particle production with yeast: Ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen. In: Microbial Cell Factories 10 (2011), 48. DOI: http://dx.doi.org/10.1186/1475-2859-10-48
http://www.repo.uni-hannover.de/handle/123456789/707
http://dx.doi.org/10.15488/683
Background: A protective immune response against Hepatitis B infection can be obtained through the administration of a single viral polypeptide, the Hepatitis B surface antigen (HBsAg). Thus, the Hepatitis B vaccine is generated through the utilization of recombinant DNA technology, preferentially by using yeast-based expression systems. However, the polypeptide needs to assemble into spherical particles, so-called virus-like particles (VLPs), to elicit the required protective immune response. So far, no clear evidence has been presented showing whether HBsAg assembles in vivo inside the yeast cell into VLPs or later in vitro during down-stream processing and purification.Results: High level production of HBsAg was carried out with recombinant Pichia pastoris using the methanol inducible AOX1 expression system. The recombinant vaccine was isolated in form of VLPs after several down-stream steps from detergent-treated cell lysates. Search for the intracellular localization of the antigen using electron microscopic studies in combination with immunogold labeling revealed the presence of HBsAg in an extended endoplasmic reticulum where it was found to assemble into defined multi-layered, lamellar structures. The distance between two layers was determined as ~6 nm indicating that these lamellas represent monolayers of well-ordered HBsAg subunits. We did not find any evidence for the presence of VLPs within the endoplasmic reticulum or other parts of the yeast cell.Conclusions: It is concluded that high level production and intrinsic slow HBsAg VLP assembly kinetics are leading to retention and accumulation of the antigen in the endoplasmic reticulum where it assembles at least partly into defined lamellar structures. Further transport of HBsAg to the Golgi apparatus is impaired thus leading to secretory pathway disfunction and the formation of an extended endoplasmic reticulum which bulges into irregular cloud-shaped formations. As VLPs were not found within the cells it is concluded that the VLP assembly process must take place during down-stream processing after detergent-mediated disassembly of HBsAg lamellas and subsequent reassembly of HBsAg into spherical VLPs.
Made available in DSpace on 2016-11-09T10:37:58Z (GMT). No. of bitstreams: 0
Previous issue date: 2011
Helmholtz Centre for Infection Research
DBT (India)
BMBF
publishedVersion
eng
London : BioMed Central Ltd.
Microbial Cell Factories 10 (2011)
1475-2859
http://dx.doi.org/10.1186/1475-2859-10-48
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
aldehyde oxidase
hepatitis B surface antigen
hepatitis B surface antigen
methanol
pharmacology
recombinant vaccine
virus like particle vaccine
article
article
biosynthesis
cell ultrastructure
cellular distribution
chemistry
controlled study
crystal structure
downstream processing
electron microscopy
endoplasmic reticulum
endoplasmic reticulum
fungal strain
gene expression
genetics
immunocytochemistry
immunogold labeling
immunohistochemistry
immunology
metabolism
nonhuman
Pichia
Pichia pastoris
protein localization
protein synthesis
transmission electron microscopy
virus like agent
Aldehyde Oxidase
biosynthesis
biosynthesis
biosynthesis
chemistry
Endoplasmic Reticulum
genetics
genetics
genetics
genetics
Hepatitis B Surface Antigens
Immunohistochemistry
immunology
immunology
immunology
metabolism
metabolism
metabolism
Methanol
Microscopy, Electron, Transmission
pharmacology
Pichia
Vaccines, Synthetic
Vaccines, Virus-Like Particle
Pichia pastoris
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Virus-like particle production with yeast: Ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen
Article
Text
10
48
openAccess
ORIGINAL
art_10.1186_1475-2859-10-48.pdf
art_10.1186_1475-2859-10-48.pdf
application/pdf
3166027
https://www.repo.uni-hannover.de/bitstream/123456789/707/1/art_10.1186_1475-2859-10-48.pdf
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MD5
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art_10.1186_1475-2859-10-48.pdf.txt
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MD5
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123456789/707
oai:www.repo.uni-hannover.de:123456789/707
2022-12-02 17:14:10.287
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/7952022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Boy, Jens
390f6697-9f9a-4d47-8002-11d13db326e2
600
Godoy, Roberto
5d7a18e5-b9ef-4940-9932-e13477c666ef
600
Shibistova, Olga
15b92bb6-8e1a-4c95-bbb7-52355b5e44a6
600
Boy, Diana
5b653000-4c62-4ff7-a3be-047e538d48b4
600
McCulloch, Robert
701d87b8-eb22-44a6-bece-00a1f3e76bfd
600
de la Fuente, Alberto Andrino
760ecdfd-13e6-4f7c-ab4c-f997a0735a44
600
Morales, Mauricio Aguirre
47ba553e-b452-4ca0-a15c-3690d2025c24
600
Mikutta, Robert
011194d0-939e-421b-bd3d-cdf0034e53d9
600
Guggenberger, Georg
32226bc2-d1b6-49d7-bae6-fda855a5f5d4
600
2016-11-30T08:54:04Z
2016-11-30T08:54:04Z
2016
Boy, Jens; Godoy, R.; Shibistova, Olga; Boy, Diana; McCulloch, R. et al.: Successional patterns along soil development gradients formed by glacier retreat in the Maritime Antarctic, King George Island. In: Revista Chilena de Historia Natural 89 (2016), 6. DOI: http://dx.doi.org/10.1186/s40693-016-0056-8
http://www.repo.uni-hannover.de/handle/123456789/795
http://dx.doi.org/10.15488/771
Background: Maritime Antarctica is severely affected by climate change and accelerating glacier retreat forming temporal gradients of soil development. Successional patterns of soil development and plant succession in the region are largely unknown, as are the feedback mechanisms between both processes. Here we identify three temporal gradients representing horizontal and vertical glacier retreat, as well as formation of raised beaches due to isostatic uplift, and describe soil formation and plant succession along them. Our hypotheses are (i) plants in Antarctica are able to modulate the two base parameters in soil development, organic C content and pH, along the temporal gradients, leading to an increase in organic carbon and soil acidity at relatively short time scales, (ii) the soil development induces succession along these gradients, and (iii) with increasing soil development, bryophytes and Deschampsia antarctica develop mycorrhiza in maritime Antarctica in order to foster interaction with soil. Results: All temporal gradients showed soil development leading to differentiation of soil horizons, carbon accumulation and increasing pH with age. Photoautptroph succession occurred rapidly after glacier retreat, but occurrences of mosses and lichens interacting with soils by rhizoids or rhizines were only observed in the later stages. The community of ground dwelling mosses and lichens is the climax community of soil succession, as the Antarctic hairgrass D. antarctica was restricted to ornithic soils. Neither D. antarctica nor mosses at the best developed soils showed any sign of mycorrhization. Conclusion: Temporal gradients formed by glacier retreat can be identified in maritime Antarctic, where soil development and plant succession of a remarkable pace can be observed, although pseudo-succession occurs by fertilization gradients caused by bird feces. Thus, the majority of ice-free surface in Antarctica is colonized by plant communities which interact with soil by litter input rather than by direct transfer of photoassimilates to soil.
Made available in DSpace on 2016-11-30T08:54:04Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
publishedVersion
eng
Santiago : Sociedad de Biologia de Chile
Revista Chilena de Historia Natural 89 (2016)
0716-078X
http://dx.doi.org/10.1186/s40693-016-0056-8
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Chronosequences
King George Island
Maritime Antarctica
Mycorrhiza
Ornithic
Soil organic carbon
Soil succession
Temporal gradients
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Successional patterns along soil development gradients formed by glacier retreat in the Maritime Antarctic, King George Island
Article
Text
89
6
openAccess
ORIGINAL
art_10.1186_s40693-016-0056-8.pdf
art_10.1186_s40693-016-0056-8.pdf
application/pdf
3305563
https://www.repo.uni-hannover.de/bitstream/123456789/795/1/art_10.1186_s40693-016-0056-8.pdf
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123456789/795
oai:www.repo.uni-hannover.de:123456789/795
2022-12-02 17:14:10.299
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/8012022-12-02T16:14:09Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessddc:590status-type:publishedVersionddc:570
Schlinkert, Hella
6bf326bc-1823-4a7c-b39e-480de3389d06
-1
Ludwig, Martin
15cb481b-f481-408f-8291-0cc737ac2151
-1
Batáry, Peter
334c0e70-1104-4944-9d00-7cd3bdd11e8d
-1
Holzschuh, Andrea
a537b612-9f0b-4995-9cda-af579341dea1
-1
Kovács-Hostyánszki, Aniko
994a5256-a0a6-4ea7-acd1-83fe2b9d0b39
-1
Tscharntke, Teja
cebf3476-d236-4c3a-ae4b-3090c078f1d0
-1
Fischer, Christina
7cf49ff1-9c96-4dd6-9067-a444ecfcc50f
-1
2016-11-30T08:54:07Z
2016-11-30T08:54:07Z
2016
Schlinkert, H.; Ludwig, Martin; Batáry, P.; Holzschuh, A.; Kovács-Hostyánszki, A. et al.: Forest specialist and generalist small mammals in forest edges and hedges. In: Wildlife Biology 22 (2016), Nr. 3, S. 86-94. DOI: http://dx.doi.org/10.2981/wlb.00176
http://www.repo.uni-hannover.de/handle/123456789/801
http://dx.doi.org/10.15488/777
Agricultural intensification often leads to fragmentation of natural habitats, such as forests, and thereby negatively affects forest specialist species. However, human introduced habitats, such as hedges, may counteract negative effects of forest fragmentation and increase dispersal, particularly of forest specialists. We studied effects of habitat type (forest edge versus hedge) and hedge isolation from forests (connected versus isolated hedge) in agricultural landscapes on abundance, species richness and community composition of mice, voles and shrews in forest edges and hedges. Simultaneously to these effects of forest edge/hedge type we analysed impacts of habitat structure, namely percentage of bare ground and forest edge/hedge width, on abundance, species richness and community composition of small mammals. Total abundance and forest specialist abundance (both driven by the most abundant species Myodes glareolus, bank vole) were higher in forest edges than in hedges, while hedge isolation had no effect. In contrast, abundance of habitat generalists was higher in isolated compared to connected hedges, with no effect of habitat type (forest edge versus hedge). Species richness as well as abundance of the most abundant habitat generalist Sorex araneus (common shrew), were not affected by habitat type or hedge isolation. Decreasing percentage of bare ground and increasing forest edge/hedge width was associated with increased abundance of forest specialists, while habitat structure was unrelated to species richness or abundance of any other group. Community composition was driven by forest specialists, which exceeded habitat generalist abundance in forest edges and connected hedges, while abundances were similar to each other in isolated hedges. Our results show that small mammal forest specialists prefer forest edges as habitats over hedges, while habitat generalists are able to use unoccupied ecological niches in isolated hedges. Consequently even isolated hedges can be marginal habitats for forest specialists and habitat generalists and thereby may increase regional farmland biodiversity.
Made available in DSpace on 2016-11-30T08:54:07Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
DFG/BA 4438/1-1
publishedVersion
eng
Hornslet : Nordic Council for Wildlife Research
Wildlife Biology 22 (2016), Nr. 3
0909-6396
http://dx.doi.org/10.2981/wlb.00176
CC BY-NC-ND 4.0 Unported
https://creativecommons.org/licenses/by-nc-nd/4.0/
Forest
natural habitats
small mammals
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::590 | Tiere (Zoologie)
590
600
Forest specialist and generalist small mammals in forest edges and hedges
Article
Text
3
22
86
94
openAccess
ORIGINAL
wlb.00176-1.pdf
wlb.00176-1.pdf
application/pdf
524710
https://www.repo.uni-hannover.de/bitstream/123456789/801/1/wlb.00176-1.pdf
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MD5
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MD5
3
123456789/801
oai:www.repo.uni-hannover.de:123456789/801
2022-12-02 17:14:09.888
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/8042022-12-02T16:11:40Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Klug, B.
94307fd4-17b4-4372-bc78-41f2ba823697
600
Kirchner, Thomas W.
5406ad15-7a6d-484c-b484-81d002ae612a
600
Horst, Walter Johannes
6d60566b-46a5-4d64-ab78-54540c24b7ed
600
2016-11-30T08:58:08Z
2016-11-30T08:58:08Z
2015
Klug, B.; Kirchner, T.W.; Horst, W.J.: Differences in aluminium accumulation and resistance between genotypes of the genus Fagopyrum. In: Agronomy 5 (2015), Nr. 3, S. 418-434. DOI: 10.3390/agronomy5030418
http://www.repo.uni-hannover.de/handle/123456789/804
http://dx.doi.org/10.15488/780
Aluminium (Al) toxicity is a major factor reducing crop productivity worldwide. There is a broad variation in intra- and inter-specific Al resistance. Whereas the Al resistance mechanisms have generally been well explored in Al-excluding plant species, Al resistance through Al accumulation and Al tolerance is not yet well understood. Therefore, a set of 94 genotypes from three Fagopyrum species with special emphasis on F. esculentum Moench were screened, with the objective of identifying genotypes with greatly differing Al accumulation capacity. The genotypes were grown in Al-enriched peat-based substrate for 21 days. Based on the Al concentration of the xylem sap, which varied by a factor of five, only quantitative but not qualitative genotypic differences in Al accumulation could be identified. Aluminium and citrate and Al and Fe concentrations in the xylem sap were positively correlated suggesting that Fe and Al are loaded into and transported in the xylem through related mechanisms. In a nutrient solution experiment using six selected F. esculentum genotypes differing in Al and citrate concentrations in the xylem sap the significant correlation between Al and iron transport in the xylem could be confirmed. Inhibition of root elongation by Al was highly significantly correlated with root oxalate-exudation and leaf Al accumulation. This suggests that Al-activated oxalate exudation and rapid transport of Al to the shoot are prerequisites for the protection of the root apoplast from Al injury and thus overall Al resistance and Al accumulation in buckwheat.
Made available in DSpace on 2016-11-30T08:58:08Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
publishedVersion
eng
Basel : MDPI AG
Agronomy 5 (2015), Nr. 3
Agronomy
2073-4395
10.3390/agronomy5030418
CC BY 4.0 Unported
http://creativecommons.org/licenses/by/4.0/
Aluminum
Buckwheat
Citrate translocation
Genotypic differences
Oxalate exudation
Tolerance
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Differences in aluminium accumulation and resistance between genotypes of the genus Fagopyrum
Article
Text
3
5
418
434
openAccess
ORIGINAL
Klug et al 2015, CC-BY, Differences in aluminium accumulation and resistance between genotypes of the genus Fagopyrum.pdf
Klug et al 2015, CC-BY, Differences in aluminium accumulation and resistance between genotypes of the genus Fagopyrum.pdf
application/pdf
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Klug et al 2015, CC-BY, Differences in aluminium accumulation and resistance between genotypes of the genus Fagopyrum.pdf.txt
Klug et al 2015, CC-BY, Differences in aluminium accumulation and resistance between genotypes of the genus Fagopyrum.pdf.txt
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https://www.repo.uni-hannover.de/bitstream/123456789/804/2/Klug%20et%20al%202015%2c%20CC-BY%2c%20Differences%20in%20aluminium%20accumulation%20and%20resistance%20between%20genotypes%20of%20the%20genus%20Fagopyrum.pdf.txt
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https://www.repo.uni-hannover.de/bitstream/123456789/804/3/Klug%20et%20al%202015%2c%20CC-BY%2c%20Differences%20in%20aluminium%20accumulation%20and%20resistance%20between%20genotypes%20of%20the%20genus%20Fagopyrum.pdf.jpg
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MD5
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123456789/804
oai:www.repo.uni-hannover.de:123456789/804
2022-12-02 17:11:40.984
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/8352022-12-13T15:12:27Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570
Guetzoyan, Lucie
ee619ea7-325c-420f-b617-bf88e68586ef
600
Ingham, Richard J.
8686f1ed-eb5a-4fda-914f-bd00979446ff
600
Nikbin, Nikzad
2edb2b07-dbe7-4efc-bbc4-e14d54c474e7
600
Rossignol, Julien
63056d2d-0247-4ff7-b9cf-32fa518dc209
600
Wolling, Michael
787ba180-25c7-496b-b318-4170ec3f77de
600
Baumert, Mark
bcefc459-997e-4fee-82be-8a790c46af74
600
Burgess-Brown, Nicola A.
4d7e4954-52eb-45bb-ab42-85f335e53012
600
Strain-Damerell, Claire M.
a3fa9da4-efa7-4875-bf45-3edbc46127e8
600
Shrestha, Leela
d86aa3a2-645a-4a22-ae5a-42e1a527b8a9
600
Brennan, Paul E.
84c9ddcb-b088-4996-aabc-2c3eb8bba6e5
600
Fedorov, Oleg
7f1af957-8cf9-4865-8445-cde46ddc618b
600
Knapp, Stefan
82539b7d-e875-47e0-b9a8-5b6b42f0ea0e
600
Ley, Steven V.
84433116-335d-4661-b448-4202b64e57d3
600
2016-12-06T10:06:44Z
2016-12-06T10:06:44Z
2014
Guetzoyan, Lucie; Ingham, Richard J.; Nikbin, Nikzad; Rossignol, Julien; Wolling, Michael et al.: Machine-assisted synthesis of modulators of the histone reader BRD9 using flow methods of chemistry and frontal affinity chromatography. In: MedChemComm 5 (2014), Nr. 4, S. 540-546. DOI: http://dx.doi.org/10.1039/C4MD00007B
http://www.repo.uni-hannover.de/handle/123456789/835
http://dx.doi.org/10.15488/811
A combination of conventional organic synthesis, remotely monitored flow synthesis and bioassay platforms, were used for the evaluation of novel inhibitors targeting bromodomains outside the well-studied bromodomain and extra terminal (BET) family, here exemplified by activity measurements on the bromodomain of BRD9 protein, a component of some tissue-specific SWi/SNF chromatin remodelling complexes. The Frontal Affinity Chromatography combined with Mass Spectrometry (FAC-MS) method proved to be reliable and results correlated well with an independent thermal shift assay.
Made available in DSpace on 2016-12-06T10:06:44Z (GMT). No. of bitstreams: 0
Previous issue date: 2014
EPSRC/EP/F069685/1
Novartis
BP Endowment
publishedVersion
eng
Cambridge : Royal Society of Chemistry
MedChemComm 5 (2014), Nr. 4
2040-2511
2040-2503
http://dx.doi.org/10.1039/C4MD00007B
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
Biochemie
FAC-MS
Frontal Affinity Chromatography
Mass Spectrometry
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Machine-assisted synthesis of modulators of the histone reader BRD9 using flow methods of chemistry and frontal affinity chromatography
Article
Text
4
5
540
546
openAccess
Nationallizenz
ORIGINAL
c4md00007b.pdf
c4md00007b.pdf
application/pdf
555042
https://www.repo.uni-hannover.de/bitstream/123456789/835/1/c4md00007b.pdf
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c4md00007b.pdf.txt
c4md00007b.pdf.txt
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https://www.repo.uni-hannover.de/bitstream/123456789/835/2/c4md00007b.pdf.txt
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123456789/835
oai:www.repo.uni-hannover.de:123456789/835
2022-12-13 16:12:27.669
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/8632022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Kaczmarek, Karina
bdad9379-aae0-48af-bbf4-f8c148d355ac
600
Horn, Ingo
20b013df-e1ea-4ec5-ae74-3bd4148584ba
600
Nehrke, Gernot
27bb7d86-d41b-46ff-a132-129210143e62
600
Bijma, Jelle
9a067f4a-7fea-4cd1-92e7-91e33b15ad02
600
2016-12-16T07:50:14Z
2016-12-16T07:50:14Z
2015
Kaczmarek, K.; Horn, I.; Nehrke, G.; Bijma, J.: Simultaneous determination of δ11B and B/Ca ratio in marine biogenic carbonates at nanogram level. In: Chemical Geology 392 (2015), S. 32-42. DOI: https://doi.org/10.1016/j.chemgeo.2014.11.011
http://www.repo.uni-hannover.de/handle/123456789/863
http://dx.doi.org/10.15488/839
In this study we introduce a new in situ technique which allows the determination of the boron isotopic composition and B/Ca ratios simultaneously at the nanogram level using a combination of optical emission spectroscopy and multiple ion counting MC ICP-MS with laser ablation. This technique offers a new application in the paleo-field of oceanography and climatology since small samples like e.g. single foraminiferal shells can be analyzed. The simultaneous determination of the boron isotopic composition and B/Ca ratios provides two independent proxies which allow the reconstruction of the full carbonate system. To test the new technique we performed measurements on the cultured, benthic foraminifer Amphistegina lessonii. Our results yielded an average boron isotopic composition δ11B=18.0±0.83‰ (SD) with an average internal precision of 0.52‰ (RSE). The boron concentration was 53±7μg/g (SD). These results agree with the range reported in the literature. The reconstructed mean pH value is in excellent agreement with the measured pH of the seawater in which the foraminifers grew. The analysis of a foraminifer consumed approximately 1200. ng calcium carbonate containing ca. 0.06. ng boron. Compared to bulk analytical methods, this new technique requires less material and reduces the time for sample preparation.
Made available in DSpace on 2016-12-16T07:50:14Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
DFG/BI 432/7-1
publishedVersion
eng
Amsterdam : Elsevier
Chemical Geology 392 (2015)
00092541
https://doi.org/10.1016/j.chemgeo.2014.11.011
CC BY-NC-ND 3.0 Unported
https://creativecommons.org/licenses/by-nc-nd/3.0/
B/Ca
Laser ablation MC ICP-MS
Ablation
Calcium carbonate
Emission spectroscopy
Inductively coupled plasma mass spectrometry
Isotopes
Laser ablation
Mass spectrometers
Optical emission spectroscopy
Benthic foraminifera
Boron concentrations
Boron isotopes
Corals
Foraminiferal shells
Foraminifers
Multiple ion counting
Simultaneous determinations
Boron
biogenic deposit
calcium carbonate
carbonate
carbonate system
in situ measurement
isotopic composition
paleoceanography
paleoclimate
precision
reconstruction
sediment chemistry
Anthozoa
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Simultaneous determination of δ11B and B/Ca ratio in marine biogenic carbonates at nanogram level
Article
Text
392
32
42
openAccess
ORIGINAL
Kaczmarek et al 2016, CC-BY, Simultaneous determination of δ11B and B.pdf
Kaczmarek et al 2016, CC-BY, Simultaneous determination of δ11B and B.pdf
application/pdf
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https://www.repo.uni-hannover.de/bitstream/123456789/863/1/Kaczmarek%20et%20al%202016%2c%20CC-BY%2c%20Simultaneous%20determination%20of%20%ce%b411B%20and%20B.pdf
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Kaczmarek et al 2016, CC-BY, Simultaneous determination of δ11B and B.pdf.txt
Kaczmarek et al 2016, CC-BY, Simultaneous determination of δ11B and B.pdf.txt
Extracted Text
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https://www.repo.uni-hannover.de/bitstream/123456789/863/2/Kaczmarek%20et%20al%202016%2c%20CC-BY%2c%20Simultaneous%20determination%20of%20%ce%b411B%20and%20B.pdf.txt
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https://www.repo.uni-hannover.de/bitstream/123456789/863/3/Kaczmarek%20et%20al%202016%2c%20CC-BY%2c%20Simultaneous%20determination%20of%20%ce%b411B%20and%20B.pdf.jpg
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MD5
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123456789/863
oai:www.repo.uni-hannover.de:123456789/863
2022-12-02 17:14:10.505
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/8902022-12-02T15:04:49Zcom_123456789_1col_123456789_4doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Reinecke, Tobias
e306c96f-4462-4496-b54e-e4dcc7f59ae1
600
Biechele, Philipp
2d6235db-de07-4e31-9b71-148d77209563
600
Schulte, V.
4bd7f91b-4ee4-4847-ba5c-26308c839333
600
Scheper, Thomas
05a38e4d-93b1-43ab-8ffc-43e89bb8a642
600
Zimmermann, Stefan
2427dd2f-81f3-4a00-97f5-d99cc823f1aa
600
2016-12-16T10:43:00Z
2016-12-16T10:43:00Z
2015
Reinecke, T.; Biechele, P.; Schulte, V.; Scheper, T.; Zimmermann, S.: Low-cost sensor system for non-invasive monitoring of cell growth in disposable bioreactors. In: Procedia Engineering 120 (2015), S. 548-551. DOI: https://doi.org/10.1016/j.proeng.2015.08.712
http://www.repo.uni-hannover.de/handle/123456789/890
http://dx.doi.org/10.15488/866
To ensure productivity and product quality, the parameters of biotechnological processes need to be monitored. Along temperature or pH, one important parameter is the cell density in the culture medium. In this work, we present a low-cost sensor system for online cell growth monitoring in bioreactors via permittivity measurements based on coplanar transmission lines. To evaluate the sensor, E. coli cultivations are performed. We found a good correlation between optical density of the culture medium and the effective permittivity at a frequency of 1 kHz when the sensor is submerged into the culture medium. Measurements at higher frequencies additionally allow monitoring the osmolarity. Furthermore, an improved sensor was successfully used for first noninvasive measurements through the polymer wall of a disposable bioreactor.
Made available in DSpace on 2016-12-16T10:43:00Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
publishedVersion
eng
Amsterdam : Elsevier
Procedia Engineering 120 (2015)
18777058
https://doi.org/10.1016/j.proeng.2015.08.712
CC BY-NC-ND 4.0 Unported
https://creativecommons.org/licenses/by-nc-nd/4.0/
Cell growth monitoring
Single use bioreactor
Bioconversion
Bioreactors
Cells
Cytology
Dielectric spectroscopy
Escherichia coli
Growth kinetics
Optical correlation
Permittivity
Permittivity measurement
Transmission line theory
Biotechnological process
Coplanar transmission lines
Disposable bioreactors
Effective permittivity
Growth monitoring
Non-invasive monitoring
Noninvasive measurements
Single use
Cell growth
Konferenzschrift
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Low-cost sensor system for non-invasive monitoring of cell growth in disposable bioreactors
Article
Text
120
548
551
openAccess
29th European Conference on Solid-State Transducers, EUROSENSORS 2015, 6-9 September 2015, Freiburg, Germany
ORIGINAL
Reinecke et al 2015, CC-BY-NC-ND, Low-cost sensor system for non-invasive monitoring of cell growth in disposable bioreactors.pdf
Reinecke et al 2015, CC-BY-NC-ND, Low-cost sensor system for non-invasive monitoring of cell growth in disposable bioreactors.pdf
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Reinecke et al 2015, CC-BY-NC-ND, Low-cost sensor system for non-invasive monitoring of cell growth in disposable bioreactors.pdf.txt
Reinecke et al 2015, CC-BY-NC-ND, Low-cost sensor system for non-invasive monitoring of cell growth in disposable bioreactors.pdf.txt
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https://www.repo.uni-hannover.de/bitstream/123456789/890/2/Reinecke%20et%20al%202015%2c%20CC-BY-NC-ND%2c%20Low-cost%20sensor%20system%20for%20non-invasive%20monitoring%20of%20cell%20growth%20in%20disposable%20bioreactors.pdf.txt
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https://www.repo.uni-hannover.de/bitstream/123456789/890/3/Reinecke%20et%20al%202015%2c%20CC-BY-NC-ND%2c%20Low-cost%20sensor%20system%20for%20non-invasive%20monitoring%20of%20cell%20growth%20in%20disposable%20bioreactors.pdf.jpg
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MD5
3
123456789/890
oai:www.repo.uni-hannover.de:123456789/890
2022-12-02 16:04:49.487
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/8912022-12-02T15:03:40Zcom_123456789_1col_123456789_3doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Lokesha
f4060da6-5397-452d-a980-43ee55e551f4
600
Kerpen, Nils B.
85a68ca8-0ee5-40ba-9293-11eb17ca5cac
600
Sannasiraj, S.A.
860293ab-e491-4eec-964b-099cd759494d
600
Sundar, V.
0c8d2aef-6138-4c60-bda3-6d680e3d7043
600
Schlurmann, Torsten
8c553798-827c-4e40-9f83-3205a17de36d
600
2016-12-16T10:43:01Z
2016-12-16T10:43:01Z
2015
Lokesha; Kerpen, N.B.; Sannasiraj, S.A.; Sundar, V.; Schlurmann, T.: Experimental investigations on wave transmission at submerged breakwater with smooth and stepped slopes. In: Procedia Engineering 116 (2015), Nr. 1, S. 713-719. DOI: https://doi.org/10.1016/j.proeng.2015.08.356
http://www.repo.uni-hannover.de/handle/123456789/891
http://dx.doi.org/10.15488/867
Submerged breakwaters are gaining more popularity as a potential coastal protection structure resulting in moderate wave transmission with significant wave energy dissipation. Submerged breakwaters are mainly adopted to prevent erosion and to dissipate the incident wave energy. In addition, the premature wave breaking facilitates the wave surfing activities by proper designing of submerged breakwater. In the present study, the experiments are conducted on submerged breakwaters in a two dimensional wave flume to investigate the influence of stepped and smooth front slope of the submerged breakwater, its height and width in reducing wave energy. A total number of eighteen sets of experiments has been conducted for three different breakwater heights (31cm, 28cm and 26cm) and three different breakwater widths (10cm, 20cm and 30cm) with stepped and smooth front slope of breakwater. The submerged breakwater models are subjected to regular waves of four different wave heights and five different wave periods in a constant water depth of 31cm, to determine wave transmissions characteristics. The influence of relative breakwater width, relative depth of submergence of the breakwater and roughness of breakwater front slope on wave transmission are analyzed and discussed in this paper.
Made available in DSpace on 2016-12-16T10:43:01Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
DAAD
publishedVersion
eng
Amsterdam : Elsevier
Procedia Engineering 116 (2015), Nr. 1
18777058
https://doi.org/10.1016/j.proeng.2015.08.356
CC BY-NC-ND 4.0 Unported
https://creativecommons.org/licenses/by-nc-nd/4.0/
Relative depth of submergence of breakwater
Transmission
Coastal engineering
Energy dissipation
Floating breakwaters
Shore protection
Transmissions
Water waves
Wave energy conversion
Wave propagation
Wave transmission
Breakwater height
Coastal protection
Experimental investigations
Incident wave energy
Relative breakwater width
Submerged breakwater
Two-dimensional waves
Wave energy dissipation
Breakwaters
Konferenzschrift
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Experimental investigations on wave transmission at submerged breakwater with smooth and stepped slopes
Article
Text
1
116
713
719
openAccess
5th International Conference on Asian and Pacific Coasts, APAC 2009, 13-16 October 2009, Singapore
ORIGINAL
Lokesha et al 2015, CC-BY-NC-ND, Experimental investigations on wave transmission at submerged breakwater with smooth and stepped slopes.pdf
Lokesha et al 2015, CC-BY-NC-ND, Experimental investigations on wave transmission at submerged breakwater with smooth and stepped slopes.pdf
application/pdf
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Lokesha et al 2015, CC-BY-NC-ND, Experimental investigations on wave transmission at submerged breakwater with smooth and stepped slopes.pdf.txt
Lokesha et al 2015, CC-BY-NC-ND, Experimental investigations on wave transmission at submerged breakwater with smooth and stepped slopes.pdf.txt
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https://www.repo.uni-hannover.de/bitstream/123456789/891/2/Lokesha%20et%20al%202015%2c%20CC-BY-NC-ND%2c%20Experimental%20investigations%20on%20wave%20transmission%20at%20submerged%20breakwater%20with%20smooth%20and%20stepped%20slopes.pdf.txt
79286e9401d0eee877a090dbc2d23f1f
MD5
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MD5
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123456789/891
oai:www.repo.uni-hannover.de:123456789/891
2022-12-02 16:03:40.332
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/8932022-12-02T16:11:40Zcom_123456789_1col_123456789_8doc-type:Articleddc:540doc-type:Textopen_accessstatus-type:publishedVersionddc:570
Schnecker, Jörg
bbdff247-031d-4761-be64-b301808b0a5b
600
Wild, Birgit
4f63a351-d063-4871-966b-28e9d0b7a1c0
600
Takriti, Mounir
229389d4-4c04-4739-8137-c6efeee4fa4d
600
Eloy Alves, Ricardo J.
5237825f-3546-469a-85f7-d2e5ec284316
600
Gentsch, Norman
60fa96f4-8f1e-4f7f-9d7b-cfd5518c5f39
600
Gittel, Antje
92a67e11-08b2-475a-a2f5-81e064201ce5
600
Hofer, Angelika
d1d2d4c0-868b-43bd-9860-ff731da3ed91
600
Klaus, Karoline
114a171b-bb13-4b34-b449-27523b62f6c4
600
Knoltsch, Anna
0cc5140b-95e2-4e68-931e-ac424942ec7c
600
Lashchinskiy, Nikolay
f5086172-b268-4f81-8097-673f3e890ae1
600
Mikutta, Robert
011194d0-939e-421b-bd3d-cdf0034e53d9
600
Richter, Andreas
52d71f9c-5e93-47ae-9872-02fab3c97c0c
600
2016-12-16T10:43:01Z
2016-12-16T10:43:01Z
2015
Schnecker, J.; Wild, B.; Takriti, M.; Eloy Alves, R.J.; Gentsch, N. et al.: Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia. In: Soil Biology and Biochemistry 83 (2015), S. 106-115. DOI: https://doi.org/10.1016/j.soilbio.2015.01.016
http://www.repo.uni-hannover.de/handle/123456789/893
http://dx.doi.org/10.15488/869
Soil horizons below 30cm depth contain about 60% of the organic carbon stored in soils. Although insight into the physical and chemical stabilization of soil organic matter (SOM) and into microbial community composition in these horizons is being gained, information on microbial functions of subsoil microbial communities and on associated microbially-mediated processes remains sparse. To identify possible controls on enzyme patterns, we correlated enzyme patterns with biotic and abiotic soil parameters, as well as with microbial community composition, estimated using phospholipid fatty acid profiles. Enzyme patterns (i.e. distance-matrixes calculated from these enzyme activities) were calculated from the activities of six extracellular enzymes (cellobiohydrolase, leucine-amino-peptidase, N-acetylglucosaminidase, chitotriosidase, phosphatase and phenoloxidase), which had been measured in soil samples from organic topsoil horizons, mineral topsoil horizons, and mineral subsoil horizons from seven ecosystems along a 1500km latitudinal transect in Western Siberia. We found that hydrolytic enzyme activities decreased rapidly with depth, whereas oxidative enzyme activities in mineral horizons were as high as, or higher than in organic topsoil horizons. Enzyme patterns varied more strongly between ecosystems in mineral subsoil horizons than in organic topsoils. The enzyme patterns in topsoil horizons were correlated with SOM content (i.e., C and N content) and microbial community composition. In contrast, the enzyme patterns in mineral subsoil horizons were related to water content, soil pH and microbial community composition. The lack of correlation between enzyme patterns and SOM quantity in the mineral subsoils suggests that SOM chemistry, spatial separation or physical stabilization of SOM rather than SOM content might determine substrate availability for enzymatic breakdown. The correlation of microbial community composition and enzyme patterns in all horizons, suggests that microbial community composition shapes enzyme patterns and might act as a modifier for the usual dependency of decomposition rates on SOM content or C/N ratios.
Made available in DSpace on 2016-12-16T10:43:01Z (GMT). No. of bitstreams: 0
Previous issue date: 2015
Austrian Science Fund
publishedVersion
eng
Amsterdam : Elsevier
Soil Biology and Biochemistry 83 (2015)
00380717
https://doi.org/10.1016/j.soilbio.2015.01.016
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Amino acids
Ecology
Ecosystems
Enzyme activity
Fatty acids
Forestry
Microorganisms
Minerals
Organic carbon
Permafrost
Phospholipids
Soil surveys
Soils
Stabilization
Boreal forests
Extracellular enzymes
PLFA
Steppe
Tundra
Enzymes
boreal forest
community composition
enzyme activity
latitudinal gradient
microbial community
permafrost
soil depth
soil horizon
soil microorganism
subsoil
topsoil
Siberia
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
540
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia
Article
Text
83
106
115
openAccess
ORIGINAL
Schnecker et al 2015, CC-BY, Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia.pdf
Schnecker et al 2015, CC-BY, Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia.pdf
application/pdf
1930374
https://www.repo.uni-hannover.de/bitstream/123456789/893/1/Schnecker%20et%20al%202015%2c%20CC-BY%2c%20Microbial%20community%20composition%20shapes%20enzyme%20patterns%20in%20topsoil%20and%20subsoil%20horizons%20along%20a%20latitudinal%20transect%20in%20Western%20Siberia.pdf
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MD5
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TEXT
Schnecker et al 2015, CC-BY, Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia.pdf.txt
Schnecker et al 2015, CC-BY, Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia.pdf.txt
Extracted Text
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48963
https://www.repo.uni-hannover.de/bitstream/123456789/893/2/Schnecker%20et%20al%202015%2c%20CC-BY%2c%20Microbial%20community%20composition%20shapes%20enzyme%20patterns%20in%20topsoil%20and%20subsoil%20horizons%20along%20a%20latitudinal%20transect%20in%20Western%20Siberia.pdf.txt
8bb27401df3debcb465b6afec3b9ae71
MD5
2
THUMBNAIL
Schnecker et al 2015, CC-BY, Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia.pdf.jpg
Schnecker et al 2015, CC-BY, Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia.pdf.jpg
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https://www.repo.uni-hannover.de/bitstream/123456789/893/3/Schnecker%20et%20al%202015%2c%20CC-BY%2c%20Microbial%20community%20composition%20shapes%20enzyme%20patterns%20in%20topsoil%20and%20subsoil%20horizons%20along%20a%20latitudinal%20transect%20in%20Western%20Siberia.pdf.jpg
7235e95777bc269826036f96e7c0682e
MD5
3
123456789/893
oai:www.repo.uni-hannover.de:123456789/893
2022-12-02 17:11:40.887
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/9852022-12-02T16:14:09Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Ag, Didem
9b973ccb-5a6b-4af3-bd91-5269ab12e305
600
Bongartz, Rebecca
50efe5d0-4ccd-449d-a06f-37cee9f61333
600
Dogan, Leyla Eral
2ef08e48-40d8-4916-be2b-83048dd4461f
600
Seleci, Muharrem
aaeb24a0-cbb8-4d5f-88ec-23536ad1d52c
600
Walter, Johanna-Gabriela
77c4fa06-d60f-4323-8d2f-1508eb19e9d6
600
Demirkol, Dilek Odaci
030d00d7-8bd4-496c-b5a8-02f916bf1a27
600
Stahl, Frank
9e2ff2ed-280d-46d6-a902-35bd40adfecd
600
Ozcelik, Serdar
87dd51be-99ae-41db-b166-b910645325c8
600
Timur, Suna
65638e4e-ea97-42ff-a846-d3e1becb6604
600
Scheper, Thomas
05a38e4d-93b1-43ab-8ffc-43e89bb8a642
600
2016-12-22T07:48:54Z
2016-12-22T07:48:54Z
2014
Ag, D.; Bongartz, R.; Dogan, L.E.; Seleci, M.; Walter, J.-G.; et al.: Biofunctional quantum dots as fluorescence probe for cell-specific targeting. In: Colloids and Surfaces B: Biointerfaces 114 (2014), S. 96-103. DOI: https://doi.org/10.1016/j.colsurfb.2013.09.033
http://www.repo.uni-hannover.de/handle/123456789/985
http://dx.doi.org/10.15488/961
We describe here the synthesis, characterization, bioconjugation, and application of water-soluble thioglycolic acid TGA-capped CdTe/CdS quantum dots (TGA-QDs) for targeted cellular imaging. Anti-human epidermal growth factor receptor 2 (HER2) antibodies were conjugated to TGA-QDs to target HER2-overexpressing cancer cells. TGA-QDs and TGA-QDs/anti-HER2 bioconjugates were characterized by fluorescence and UV-Vis spectroscopy, X-ray diffraction (XRD), hydrodynamic sizing, electron microscopy, and gel electrophoresis. TGA-QDs and TGA-QDs/anti-HER2 were incubated with cells to examine cytotoxicity, targeting efficiency, and cellular localization. The cytotoxicity of particles was measured using an MTT assay and the no observable adverse effect concentration (NOAEC), 50% inhibitory concentration (IC50), and total lethal concentration (TLC) were calculated. To evaluate localization and targeting efficiency of TGA-QDs with or without antibodies, fluorescence microscopy and flow cytometry were performed. Our results indicate that antibody-conjugated TGA-QDs are well-suited for targeted cellular imaging studies.
Made available in DSpace on 2016-12-22T07:48:54Z (GMT). No. of bitstreams: 0
Previous issue date: 2014
TUBITAK/109T573
BMBF/01DL12013
Ege University Scientific Research/2012/FEN/0071
publishedVersion
eng
Amsterdam : Elsevier
Colloids and Surfaces B: Biointerfaces 114 (2014)
09277765
https://doi.org/10.1016/j.colsurfb.2013.09.033
CC BY-NC-ND 3.0 Unported
https://creativecommons.org/licenses/by-nc-nd/3.0/
Anti-HER2
Bioconjugation
Cell specific targeting
Imaging
Quantum dots
Electrophoresis
Fluorescence
Fluorescence microscopy
Imaging techniques
Semiconductor quantum dots
Ultraviolet visible spectroscopy
X ray diffraction
Anti-HER2
Bio-conjugation
Cellular localization
Epidermal growth factor receptor 2
Fluorescence probes
Gel electrophoresis
Inhibitory concentration
Lethal concentration
Antibodies
3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
cadmium
epidermal growth factor receptor 2
epidermal growth factor receptor 2 antibody
quantum dot
receptor antibody
sulfur
tellurium
thioglycolic acid
unclassified drug
animal cell
cancer cell
cancer diagnosis
cell assay
cell labeling
cell specificity
cell surface
cellular distribution
controlled study
drug concentration
drug conjugation
drug cytotoxicity
drug design
drug solubility
drug synthesis
electron microscopy
flow cytometry
fluorescence imaging
fluorescence microscopy
human cell
hydrodynamics
internalization
lung cancer
mouse
no observable adverse effect concentration
nonhuman
priority journal
protein expression
target cell
total lethal concentration
ultraviolet spectroscopy
X ray diffraction
Anti-HER2
Cell specific targeting
Cadmium Compounds
Cell Death
Cell Membrane
Cell Survival
Cells
Fluorescent Dyes
Light
Mice
Microscopy, Fluorescence
NIH 3T3 Cells
Receptor, erbB-2
Reproducibility of Results
Tellurium
Thioglycolates
X-Ray Diffraction
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Biofunctional quantum dots as fluorescence probe for cell-specific targeting
Article
Text
114
96
103
openAccess
ORIGINAL
Ag et al 2014, CC-BY-NC-ND, Biofunctional quantum dots as fluorescence probe for cell-specific targeting.pdf
Ag et al 2014, CC-BY-NC-ND, Biofunctional quantum dots as fluorescence probe for cell-specific targeting.pdf
application/pdf
2215123
https://www.repo.uni-hannover.de/bitstream/123456789/985/1/Ag%20et%20al%202014%2c%20CC-BY-NC-ND%2c%20Biofunctional%20quantum%20dots%20as%20fluorescence%20probe%20for%20cell-specific%20targeting.pdf
e47e3addec9325d52330dce08ea19ca4
MD5
1
TEXT
Ag et al 2014, CC-BY-NC-ND, Biofunctional quantum dots as fluorescence probe for cell-specific targeting.pdf.txt
Ag et al 2014, CC-BY-NC-ND, Biofunctional quantum dots as fluorescence probe for cell-specific targeting.pdf.txt
Extracted Text
text/plain
40604
https://www.repo.uni-hannover.de/bitstream/123456789/985/2/Ag%20et%20al%202014%2c%20CC-BY-NC-ND%2c%20Biofunctional%20quantum%20dots%20as%20fluorescence%20probe%20for%20cell-specific%20targeting.pdf.txt
7148b8ef64014137271f3d4663705179
MD5
2
THUMBNAIL
Ag et al 2014, CC-BY-NC-ND, Biofunctional quantum dots as fluorescence probe for cell-specific targeting.pdf.jpg
Ag et al 2014, CC-BY-NC-ND, Biofunctional quantum dots as fluorescence probe for cell-specific targeting.pdf.jpg
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image/jpeg
1697
https://www.repo.uni-hannover.de/bitstream/123456789/985/3/Ag%20et%20al%202014%2c%20CC-BY-NC-ND%2c%20Biofunctional%20quantum%20dots%20as%20fluorescence%20probe%20for%20cell-specific%20targeting.pdf.jpg
0c8fc86be8cb0f51c2f97cefbd7d351a
MD5
3
123456789/985
oai:www.repo.uni-hannover.de:123456789/985
2022-12-02 17:14:09.704
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/9942022-12-02T16:16:27Zcom_123456789_1col_123456789_9doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570
Müller, Katharina F.
c22a5ad2-3378-4408-a04e-c40132145f24
-1
Briel, Matthias
f079794e-6f41-472b-924e-e638c74cbf77
-1
Strech, Daniel
4a3eef44-b256-43a8-a4c6-a9de37bdd32a
-1
Meerpohl, Jörg J.
54ea6941-41bd-4846-bd29-7045164728d7
-1
Lang, Britta
e66ec099-d0ef-423b-b108-43239c60fd7f
-1
Motschall, Edith
354c4858-83dc-4396-be7c-6858093c29d0
-1
Gloy, Viktoria
25665113-0fe7-43dd-8ac2-09f99614fe8a
-1
Lamontagne, Francois
e6a56b56-bc29-407f-966e-6dfdfade504b
-1
Bassler, Dirk
6cd27170-3977-42db-acb9-6c058167bae9
-1
2016-12-22T07:48:57Z
2016-12-22T07:48:57Z
2014
Mueller, K.F.; Briel, M.; Strech, D.; Meerpohl, J.J.; Lang, B.; et al.: Dissemination bias in systematic reviews of animal research: A systematic review. In: PLoS ONE 9 (2014), Nr. 12, e116016. DOI: https://doi.org/10.1371/journal.pone.0116016
http://www.repo.uni-hannover.de/handle/123456789/994
http://dx.doi.org/10.15488/970
Background: Systematic reviews of preclinical studies, in vivo animal experiments in particular, can influence clinical research and thus even clinical care. Dissemination bias, selective dissemination of positive or significant results, is one of the major threats to validity in systematic reviews also in the realm of animal studies. We conducted a systematic review to determine the number of published systematic reviews of animal studies until present, to investigate their methodological features especially with respect to assessment of dissemination bias, and to investigate the citation of preclinical systematic reviews on clinical research. Methods: Eligible studies for this systematic review constitute systematic reviews that summarize in vivo animal experiments whose results could be interpreted as applicable to clinical care. We systematically searched Ovid Medline, Embase, ToxNet, and ScienceDirect from 1st January 2009 to 9th January 2013 for eligible systematic reviews without language restrictions. Furthermore we included articles from two previous systematic reviews by Peters et al. and Korevaar et al. Results: The literature search and screening process resulted in 512 included full text articles. We found an increasing number of published preclinical systematic reviews over time. The methodological quality of preclinical systematic reviews was low. The majority of preclinical systematic reviews did not assess methodological quality of the included studies (71%), nor did they assess heterogeneity (81%) or dissemination bias (87%). Statistics quantifying the importance of clinical research citing systematic reviews of animal studies showed that clinical studies referred to the preclinical research mainly to justify their study or a future study (76%). Discussion: Preclinical systematic reviews may have an influence on clinical research but their methodological quality frequently remains low. Therefore, systematic reviews of animal research should be critically appraised before translating them to a clinical context.
Made available in DSpace on 2016-12-22T07:48:57Z (GMT). No. of bitstreams: 0
Previous issue date: 2014
publishedVersion
eng
San Francisco : Public Library of Science
PLoS ONE 9 (2014)
19326203
https://doi.org/10.1371/journal.pone.0116016
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
animal experiment
clinical research
dissemination bias
in vivo study
methodology
nonhuman
publication
quality control
Review
systematic error
systematic review (topic)
drug screening
literature
meta analysis (topic)
publishing
Animalia
Animals
Drug Evaluation, Preclinical
Publication Bias
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dissemination bias in systematic reviews of animal research: A systematic review
Article
Text
12
9
e116016
openAccess
ORIGINAL
Mueller et al 2014, CC BY, Dissemination bias in systematic reviews of animal research A systematic review.pdf
Mueller et al 2014, CC BY, Dissemination bias in systematic reviews of animal research A systematic review.pdf
application/pdf
529031
https://www.repo.uni-hannover.de/bitstream/123456789/994/1/Mueller%20et%20al%202014%2c%20CC%20BY%2c%20Dissemination%20bias%20in%20systematic%20reviews%20of%20animal%20research%20A%20systematic%20review.pdf
51c81306bf9a83aec36c30b76a8f17ad
MD5
1
TEXT
Mueller et al 2014, CC BY, Dissemination bias in systematic reviews of animal research A systematic review.pdf.txt
Mueller et al 2014, CC BY, Dissemination bias in systematic reviews of animal research A systematic review.pdf.txt
Extracted Text
text/plain
41150
https://www.repo.uni-hannover.de/bitstream/123456789/994/2/Mueller%20et%20al%202014%2c%20CC%20BY%2c%20Dissemination%20bias%20in%20systematic%20reviews%20of%20animal%20research%20A%20systematic%20review.pdf.txt
85d7b611d2aeebceafdf1c4547031f91
MD5
2
THUMBNAIL
Mueller et al 2014, CC BY, Dissemination bias in systematic reviews of animal research A systematic review.pdf.jpg
Mueller et al 2014, CC BY, Dissemination bias in systematic reviews of animal research A systematic review.pdf.jpg
Generated Thumbnail
image/jpeg
1861
https://www.repo.uni-hannover.de/bitstream/123456789/994/3/Mueller%20et%20al%202014%2c%20CC%20BY%2c%20Dissemination%20bias%20in%20systematic%20reviews%20of%20animal%20research%20A%20systematic%20review.pdf.jpg
faab985d8421c86408f1c7ed85132e06
MD5
3
123456789/994
oai:www.repo.uni-hannover.de:123456789/994
2022-12-02 17:16:27.274
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/10342022-12-02T16:16:27Zcom_123456789_1col_123456789_9doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570
Schmitz, Gerd
edb823fd-af28-4724-aa70-ea80e62189a1
600
Mohammadi, Bahram
92dced34-c469-4779-9cd0-680912ab812d
600
Hammer, Anke
a7e249cc-fc3c-4060-9b86-b197e623ae53
600
Heldmann, Marcus
6c5b2457-f2db-41b4-8b5f-755431a478fd
600
Samii, Amir
0293d14a-3a4b-4ddc-9d3c-fffe16411472
600
Münte, Thomas F.
8cbcad6a-f263-46ae-b3e5-be201a65f092
600
Effenberg, Alfred O.
37516a07-bbdb-4455-b007-4cfff2838f61
600
2016-12-22T11:58:59Z
2016-12-22T11:58:59Z
2013
Schmitz, G.; Mohammadi, B.; Hammer, A.; Heldmann, M.; Samii, A.; et al.: Observation of sonified movements engages a basal ganglia frontocortical network. In: BMC Neuroscience 14 (2013), 32. DOI: https://doi.org/10.1186/1471-2202-14-32
http://www.repo.uni-hannover.de/handle/123456789/1034
http://dx.doi.org/10.15488/1010
Background: Producing sounds by a musical instrument can lead to audiomotor coupling, i.e. the joint activation of the auditory and motor system, even when only one modality is probed. The sonification of otherwise mute movements by sounds based on kinematic parameters of the movement has been shown to improve motor performance and perception of movements.Results: Here we demonstrate in a group of healthy young non-athletes that congruently (sounds match visual movement kinematics) vs. incongruently (no match) sonified breaststroke movements of a human avatar lead to better perceptual judgement of small differences in movement velocity. Moreover, functional magnetic resonance imaging revealed enhanced activity in superior and medial posterior temporal regions including the superior temporal sulcus, known as an important multisensory integration site, as well as the insula bilaterally and the precentral gyrus on the right side. Functional connectivity analysis revealed pronounced connectivity of the STS with the basal ganglia and thalamus as well as frontal motor regions for the congruent stimuli. This was not seen to the same extent for the incongruent stimuli.Conclusions: We conclude that sonification of movements amplifies the activity of the human action observation system including subcortical structures of the motor loop. Sonification may thus be an important method to enhance training and therapy effects in sports science and neurological rehabilitation.
Made available in DSpace on 2016-12-22T11:58:59Z (GMT). No. of bitstreams: 0
Previous issue date: 2013
DFG/SFB/TR31/EU
DFG/TP/A7/EU
publishedVersion
eng
London : BioMed Central Ltd.
info:eu-repo/grantAgreement/DFG/SFB/TR31/EU
info:eu-repo/grantAgreement/DFG/TP/A7/EU
BMC Neuroscience 14 (2013)
14712202
https://doi.org/10.1186/1471-2202-14-32
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
adult
basal ganglion
connectome
female
functional magnetic resonance imaging
human
human experiment
inferior temporal cortex
male
nerve cell network
normal human
perception
primary motor cortex
superior temporal sulcus
temporal lobe
thalamus
velocity
Acoustics
Basal Ganglia
Biomechanics
Brain Mapping
Frontal Lobe
Image Processing, Computer-Assisted
Magnetic Resonance Imaging
Movement
Neural Pathways
Observation
Oxygen
Photic Stimulation
Reaction Time
Young Adult
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Observation of sonified movements engages a basal ganglia frontocortical network
Article
Text
14
32
openAccess
ORIGINAL
Schmitz et al 2013, CC BY, Observation of sonified movements engages a basal ganglia frontocortical network.pdf
Schmitz et al 2013, CC BY, Observation of sonified movements engages a basal ganglia frontocortical network.pdf
application/pdf
1679621
https://www.repo.uni-hannover.de/bitstream/123456789/1034/1/Schmitz%20et%20al%202013%2c%20CC%20BY%2c%20Observation%20of%20sonified%20movements%20engages%20a%20basal%20ganglia%20frontocortical%20network.pdf
7dfdfa600d4358dc7e98023924d5bd58
MD5
1
TEXT
Schmitz et al 2013, CC BY, Observation of sonified movements engages a basal ganglia frontocortical network.pdf.txt
Schmitz et al 2013, CC BY, Observation of sonified movements engages a basal ganglia frontocortical network.pdf.txt
Extracted Text
text/plain
52048
https://www.repo.uni-hannover.de/bitstream/123456789/1034/2/Schmitz%20et%20al%202013%2c%20CC%20BY%2c%20Observation%20of%20sonified%20movements%20engages%20a%20basal%20ganglia%20frontocortical%20network.pdf.txt
fde1b46cd087cbe0a85a0471c7503528
MD5
2
THUMBNAIL
Schmitz et al 2013, CC BY, Observation of sonified movements engages a basal ganglia frontocortical network.pdf.jpg
Schmitz et al 2013, CC BY, Observation of sonified movements engages a basal ganglia frontocortical network.pdf.jpg
Generated Thumbnail
image/jpeg
1711
https://www.repo.uni-hannover.de/bitstream/123456789/1034/3/Schmitz%20et%20al%202013%2c%20CC%20BY%2c%20Observation%20of%20sonified%20movements%20engages%20a%20basal%20ganglia%20frontocortical%20network.pdf.jpg
217bc6f593ea8b4c7dda09c8d9fd7602
MD5
3
123456789/1034
oai:www.repo.uni-hannover.de:123456789/1034
2022-12-02 17:16:27.528
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/10362022-12-02T16:11:40Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Ogada, Pamella Akoth
45242ba1-9abc-42e8-af44-bc7387405e19
600
Debener, Thomas
2aedee57-8d8b-49ca-9c67-1a988b8fba75
600
Poehling, Hans-Michael
84b8da68-9ed7-4a7f-a98c-29ba64f9092c
600
2017-01-04T12:59:42Z
2017-01-04T12:59:42Z
2016
Ogada, Pamella Akoth; Debener, Thomas; Poehling, Hans-Michael: Inheritance genetics of the trait vector competence in Frankliniella occidentalis (Western flower thrips) in the transmission of Tomato spotted wilt virus. In: Ecology and Evolution 6 (2016), Nr. 21, S. 7911-7920. DOI: https://doi.org/10.1002/ece3.2484
http://www.repo.uni-hannover.de/handle/123456789/1036
http://dx.doi.org/10.15488/1012
The complexity of tospovirus–vector–host plant interaction is linked to a range of factors influencing vector's efficacy in virus transmission, leading to high variability in the transmission efficiency within vector populations. Main shortcomings of most studies are the missing information on the intrinsic potential of individual insects to serve as efficient vectors, both at phenotypic and at genotypic levels. Moreover, detailed analysis of vector competence heredity and monitoring the splitting of both genotypes and phenotypes in filial generations has not been reported. In this study, using the model system Frankliniella occidentalis and Tomato spotted wilt virus, we evaluated the inheritance and stability of the trait vector competence in a population through basic crossings of individually characterized partners, as well as virgin reproduction. We hypothesized that the trait is heritable in F. occidentalis and is controlled by a recessive allele. From the results, 83% and 94% of competent and noncompetent males respectively, inherited their status from their mothers. The trait was only expressed when females were homozygous for the corresponding allele. Furthermore, the allele frequencies were different between males and females, and the competent allele had the highest frequency in the population. These suggest that the trait vector competence is inherited in single recessive gene in F. occidentalis, for which the phenotype is determined by the haplodiploid mechanism. These findings are fundamental for our understanding of the temporal and spatial variability within vector populations with respect to the trait vector competence and at the same time offer an essential basis for further molecular studies.
Made available in DSpace on 2017-01-04T12:59:42Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
DFG/207/37-1
publishedVersion
eng
Chichester : John Wiley & Sons Ltd.
Ecology and Evolution 6 (2016), Nr. 21
2045-7758
https://doi.org/10.1002/ece3.2484
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Frankliniella occidentalis
haplodiploidy
inheritance
intraspecific variation
Tomato spotted wilt virus
tospovirus
vector competence
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Inheritance genetics of the trait vector competence in Frankliniella occidentalis (Western flower thrips) in the transmission of Tomato spotted wilt virus
Article
Text
21
6
7911
7920
openAccess
LUH_Fonds
ORIGINAL
Ogada_et_al-2016-Ecology_and_Evolution.pdf
Ogada_et_al-2016-Ecology_and_Evolution.pdf
application/pdf
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Ogada_et_al-2016-Ecology_and_Evolution.pdf.txt
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57017
https://www.repo.uni-hannover.de/bitstream/123456789/1036/2/Ogada_et_al-2016-Ecology_and_Evolution.pdf.txt
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MD5
3
123456789/1036
oai:www.repo.uni-hannover.de:123456789/1036
2022-12-02 17:11:40.964
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/10452022-12-02T16:17:36Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Jędrzejuk, Agata
bb3ffaf6-d835-4dfc-8141-8d09e681d9db
600
Rabiza-Świder, Julitta
36770a5b-13e8-40e6-ae7c-a76028d67b10
600
Skutnik, Ewa
2dc9b5de-3510-4960-9856-ae80a793a95b
600
Serek, Margrethe
5414d509-c2cd-4b51-9c26-cd2042c693e0
600
2017-01-12T08:11:09Z
2017-01-12T08:11:09Z
2016
Jędrzejuk, A.; Rabiza-Świder, J.; Skutnik, E.; Serek, M.: Flowering conditions affect flower longevity in Syringa vulgaris and cause changes in protein content, protease activity and expression of a KDEL-CysEP gene. In: Acta Physiologiae Plantarum 38 (2016), Nr. 2, S. 1-11. DOI: http://dx.doi.org/10.1007/s11738-015-2044-z
http://www.repo.uni-hannover.de/handle/123456789/1045
http://dx.doi.org/10.15488/1021
Forcing is a method that is usually used to induce flowering in plants, independent of the natural blooming period. The temperatures required to start blooming in lilac in November are ca. 37°C causing degeneration of flowers. Forcing at 15 °C in November requires 49 days to bloom as compared to 23 days for the standard 37 °C, but gives panicles of much better quality than those forced by standard method (37 °C). In this study, we have investigated the protein content, total protease activity, and cysteine protease activity at different stages of flowering (flower bud whitening and swelling, open flowers, wilted flowers) for lilac flowers (Syringa vulgaris L., fam. Oleaceae) blooming under three different conditions: natural conditions in May and forcing in a greenhouse in November at 37 °C (standard forcing) or at 15 °C (alternative forcing). The protein content was relatively constant during flowering for each of the three sets of conditions. Flowers from 15 °C had a significantly lower total protease and cysteine endoprotease activity than flowers from 37 °C at all stages. Flowers from plants blooming in May had a very time-specific cysteine protease activity, which was dramatically higher for the open flower stage than for the other stages. The partial coding sequence for a KDEL-CysEP was isolated, and its expression was determined by qRT-PCR. The gene expression did not correlate with the cysteine endoprotease activity especially in May natural flowering and November alternative forcing at 15 °C. Alternative forcing method at 15 °C affected the flowering process delaying senescence, presumably due to the low cysteine protease activity. © 2016, The Author(s).
Made available in DSpace on 2017-01-12T08:11:09Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
National Center of Knowledge (NCN)
publishedVersion
eng
Heidelberg : Springer
Acta Physiologiae Plantarum 38 (2016), Nr. 2
1861-1664
0137-5881
https://doi.org/10.1007/s11738-015-2044-z
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Flowering
Forcing
Programmed cell death
Senescence
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Flowering conditions affect flower longevity in Syringa vulgaris and cause changes in protein content, protease activity and expression of a KDEL-CysEP gene
Article
Text
2
38
1
11
openAccess
ORIGINAL
art_10.1007_s11738-015-2044-z.pdf
art_10.1007_s11738-015-2044-z.pdf
application/pdf
689138
https://www.repo.uni-hannover.de/bitstream/123456789/1045/1/art_10.1007_s11738-015-2044-z.pdf
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art_10.1007_s11738-015-2044-z.pdf.txt
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text/plain
49588
https://www.repo.uni-hannover.de/bitstream/123456789/1045/2/art_10.1007_s11738-015-2044-z.pdf.txt
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MD5
3
123456789/1045
oai:www.repo.uni-hannover.de:123456789/1045
2022-12-02 17:17:36.861
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/10512022-12-02T16:17:36Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Kozak, Sandra
7623de62-a7e6-4eba-8ff9-8a424382c7ab
600
Lercher, Lukas
974e128d-bd64-4f2c-bc7e-1d217cf88c01
600
Karanth, Megha N.
0f01028a-0639-4d3c-b324-f9353f088962
600
Meijers, Rob
901544ba-6696-45ee-a117-2b6868165093
600
Carlomagno, Teresa
7265c713-64ff-46a6-bf0a-e461ea0e87d8
600
Boivin, Stephane
ef24e07c-fd81-40f6-be37-2adbc8cf9028
600
2017-01-12T08:35:29Z
2017-01-12T08:35:29Z
2016
Kozak, S.; Lercher, L.; Karanth, M.N.; Meijers, R.; Carlomagno, T. et al.: Optimization of protein samples for NMR using thermal shift assays. In: Journal of Biomolecular NMR 64 (2016), Nr. 4, S. 281-289. DOI: http://dx.doi.org/10.1007/s10858-016-0027-z
http://www.repo.uni-hannover.de/handle/123456789/1051
http://dx.doi.org/10.15488/1027
Maintaining a stable fold for recombinant proteins is challenging, especially when working with highly purified and concentrated samples at temperatures >20 °C. Therefore, it is worthwhile to screen for different buffer components that can stabilize protein samples. Thermal shift assays or ThermoFluor® provide a high-throughput screening method to assess the thermal stability of a sample under several conditions simultaneously. Here, we describe a thermal shift assay that is designed to optimize conditions for nuclear magnetic resonance studies, which typically require stable samples at high concentration and ambient (or higher) temperature. We demonstrate that for two challenging proteins, the multicomponent screen helped to identify ingredients that increased protein stability, leading to clear improvements in the quality of the spectra. Thermal shift assays provide an economic and time-efficient method to find optimal conditions for NMR structural studies. © 2016, The Author(s).
Made available in DSpace on 2017-01-12T08:35:29Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
EU/FP7/2007–2013
European Molecular Biology Laboratory
EMBO Long-term Fellowship
publishedVersion
eng
Dordrecht : Springer Netherlands
Journal of Biomolecular NMR 64 (2016), Nr. 4
1573-5001
0925-2738
https://doi.org/10.1007/s10858-016-0027-z
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Differential scanning fluorimetry
Nuclear magnetic resonance
Protein thermal stability
Sample optimization
Thermal shift assay
ThermoFluor
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Optimization of protein samples for NMR using thermal shift assays
Article
Text
4
64
281
289
openAccess
ORIGINAL
art_10.1007_s10858-016-0027-z.pdf
art_10.1007_s10858-016-0027-z.pdf
application/pdf
1410794
https://www.repo.uni-hannover.de/bitstream/123456789/1051/1/art_10.1007_s10858-016-0027-z.pdf
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33901
https://www.repo.uni-hannover.de/bitstream/123456789/1051/2/art_10.1007_s10858-016-0027-z.pdf.txt
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MD5
3
123456789/1051
oai:www.repo.uni-hannover.de:123456789/1051
2022-12-02 17:17:36.858
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/10902022-12-02T16:11:40Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Dammak, Mouna
8b7af6a3-0440-462b-badb-420e45edb754
-1
Haase, Sandra Mareike
6a2d9569-9bac-4f01-839c-503ac208bbe7
-1
Miladi, Ramzi
b641c64d-cc46-4837-a916-84da4e58ce43
-1
Ben Amor, Faten
57f75e95-b02a-49eb-8bd5-1005dccff6c6
-1
Barkallah, Mohamed
fd1f5cf2-63ff-46ba-b311-9538d02cbf76
-1
Gosset, David
479e7c20-7574-4cb5-a7fa-c5c72b69690d
-1
Pichon, Chantal
ee760552-acb6-4cd1-8fc2-45a0df110760
-1
Huchzermeyer, Bernhard
bdd750a6-fc3c-4592-95c6-5437378dd01f
-1
Fendri, Imen
61bb6d82-a13f-438b-b2d1-d7eab43daa52
-1
Denis, Michel
dd088f7f-a2a6-4066-92e1-5c89da82fa51
-1
Abdelkafi, Slim
394be8ca-b9f5-49a1-b9ab-e1b8b0296202
-1
2017-01-27T08:36:54Z
2017-01-27T08:36:54Z
2016
Dammak, M.; Haase, Sandra Mareike; Miladi, R.; Ben, Amor, F.; Barkallah, M. et al.: Enhanced lipid and biomass production by a newly isolated and identified marine microalga. In: Lipids in Health and Disease 15 (2016), Nr. 1, 209. DOI: https://doi.org/10.1186/s12944-016-0375-4
http://www.repo.uni-hannover.de/handle/123456789/1090
http://dx.doi.org/10.15488/1066
Background: The increasing demand for microalgae lipids as an alternative to fish has encouraged researchers to explore oleaginous microalgae for food uses. In this context, optimization of growth and lipid production by the marine oleaginous V2-strain-microalgae is of great interest as it contains large amounts of mono-unsaturated (MUFAs) and poly-unsaturated fatty acids (PUFAs). Methods: In this study, the isolated V2 strain was identified based on 23S rRNA gene. Growth and lipid production conditions were optimized by using the response surface methodology in order to maximize its cell growth and lipid content that was quantified by both flow cytometry and the gravimetric method. The intracellular lipid bodies were detected after staining with Nile red by epifluorescence microscopy. The fatty acid profile of optimal culture conditions was determined by gas chromatography coupled to a flame ionization detector. Results: The phenotypic and phylogenetic analyses showed that the strain V2 was affiliated to Tetraselmis genus. The marine microalga is known as an interesting oleaginous species according to its high lipid production and its fatty acid composition. The optimization process showed that maximum cell abundance was achieved under the following conditions: pH: 7, salinity: 30 and photosynthetic light intensity (PAR): 133 μmol photons.m-2.s-1. In addition, the highest lipid content (49 ± 2.1% dry weight) was obtained at pH: 7, salinity: 37.23 and photosynthetic light intensity (PAR): 188 μmol photons.m-2.s-1. The fatty acid profile revealed the presence of 39.2% and 16.1% of total fatty acids of mono-unsaturated fatty acids (MUFAs) and poly-unsaturated fatty acids (PUFAs), respectively. Omega 3 (ω3), omega 6 (ω6) and omega 9 (ω9) represented 5.28%, 8.12% and 32.8% of total fatty acids, respectively. Conclusions: This study showed the successful optimization of salinity, light intensity and pH for highest growth, lipid production and a good fatty acid composition, making strain V2 highly suitable for food and nutraceutical applications. © 2016 The Author(s).
Made available in DSpace on 2017-01-27T08:36:54Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
Tunisian Ministry of higher education and scientific research
publishedVersion
eng
London : BioMed Central Ltd.
Lipids in Health and Disease 15 (2016), Nr. 1
1476-511X
https://doi.org/10.1186/s12944-016-0375-4
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Flow cytometry
Lipids
Microalgae
Poly-unsaturated fatty acids
Response surface methodology
Tetraselmis sp.
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Enhanced lipid and biomass production by a newly isolated and identified marine microalga
Article
Text
1
15
209
openAccess
ORIGINAL
art_10.1186_s12944-016-0375-4.pdf
art_10.1186_s12944-016-0375-4.pdf
application/pdf
4434685
https://www.repo.uni-hannover.de/bitstream/123456789/1090/1/art_10.1186_s12944-016-0375-4.pdf
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MD5
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57514
https://www.repo.uni-hannover.de/bitstream/123456789/1090/2/art_10.1186_s12944-016-0375-4.pdf.txt
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MD5
3
123456789/1090
oai:www.repo.uni-hannover.de:123456789/1090
2022-12-02 17:11:40.968
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/10952022-12-02T16:14:09Zcom_123456789_1col_123456789_8doc-type:Articleddc:540doc-type:Textopen_accessstatus-type:publishedVersionddc:570
Scherer, Sabrina
dd601e56-d446-4adb-8965-99dfd33d4afd
600
Wollrab, Eva
d047ae51-fbc7-466a-92b0-8a507ed45695
600
Codutti, Luca
1e6b6eda-9b1c-4fb2-8dfc-a5370c117e34
600
Carlomagno, Teresa
7265c713-64ff-46a6-bf0a-e461ea0e87d8
600
da Costa, Stefan Gomes
90e427c3-f004-46b5-b2b0-22d2b68c1045
600
Volkmer, Andreas
baee6674-4c42-4b30-998e-0f2b61e2fafa
600
Bronja, Amela
41dbabcd-ff36-4dfb-bfda-7eb0bec5f34b
600
Schmitz, Oliver J.
d363242b-d02b-499d-9674-705d987dc7a4
600
Ott, Albrecht
8ff23678-864c-4735-a422-8f5be4a8c744
600
2017-01-27T08:36:55Z
2017-01-27T08:36:55Z
2016
Scherer, S.; Wollrab, E.; Codutti, Luca; Carlomagno, T.; da, Costa, S.G. et al.: Chemical Analysis of a “Miller-Type” Complex Prebiotic Broth: Part II: Gas, Oil, Water and the Oil/Water-Interface. In: Origins of Life and Evolution of Biospheres 2016 (2016), S. 1-23. DOI: https://doi.org/10.1007/s11084-016-9528-8
http://www.repo.uni-hannover.de/handle/123456789/1095
http://dx.doi.org/10.15488/1071
We have analyzed the chemical variety obtained by Miller-Urey-type experiments using nuclear magnetic resonance (NMR) spectroscopy and coherent anti-Stokes Raman scattering (CARS) spectroscopy, gas chromatography followed by mass spectrometry (GC/MS) and two-dimensional gas chromatography followed by mass spectrometry (GCxGC/MS). In the course of a running Miller-Urey-type experiment, a hydrophobic organic layer emerged besides the hydrophilic aqueous phase and the gaseous phase that were initially present. The gas phase mainly consisted of aromatic compounds and molecules containing C≡C or C≡N triple bonds. The hydrophilic phase contained at least a few thousands of different molecules, primarily distributed in a range of 50 and 500 Da. The hydrophobic phase is characterized by carbon-rich, oil-like compounds and their amphiphilic derivatives containing oxygen with tensioactive properties. The presence of a wide range of oxidized molecules hints to the availability of oxygen radicals. We suggest that they intervene in the formation of alkylated polyethylene glycol (PEG) in the oil/water interface. CARS spectroscopy revealed distinct vibrational molecular signatures. In particular, characteristic spectral bands for cyanide compounds were observed if the broth was prepared with electric discharges in the gaseous phase. The characteristic spectral bands were absent if discharges were released onto the water surface. NMR spectroscopy on the same set of samples independently confirmed the observation. In addition, NMR spectroscopy revealed overall high chemical variability that suggests strong non-linearities due to interdependent, sequential reaction steps. © 2016 The Author(s)
Made available in DSpace on 2017-01-27T08:36:55Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
Saarland University
HSFP
EU/FP7/HEALTH-F5-2008-200820
publishedVersion
eng
Dordrecht : Springer Netherlands
Origins of Life and Evolution of Biospheres 2016 (2016)
1573-0875
0169-6149
https://doi.org/10.1007/s11084-016-9528-8
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
coherent anti-Stokes Raman scattering (CARS)
Complex chemical mixture
GC/MS
GCxGC/MS
Miller-Urey experiment
Molecular vibrations
NMR
Oil/water interface
Origin of Life
Phase-transfer-catalysis
Radicals
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
540
600
Chemical Analysis of a “Miller-Type” Complex Prebiotic Broth: Part II: Gas, Oil, Water and the Oil/Water-Interface
Article
Text
2016
1
23
openAccess
ORIGINAL
art_10.1007_s11084-016-9528-8.pdf
art_10.1007_s11084-016-9528-8.pdf
application/pdf
2921287
https://www.repo.uni-hannover.de/bitstream/123456789/1095/1/art_10.1007_s11084-016-9528-8.pdf
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MD5
1
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art_10.1007_s11084-016-9528-8.pdf.txt
art_10.1007_s11084-016-9528-8.pdf.txt
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text/plain
54867
https://www.repo.uni-hannover.de/bitstream/123456789/1095/2/art_10.1007_s11084-016-9528-8.pdf.txt
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MD5
2
THUMBNAIL
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MD5
3
123456789/1095
oai:www.repo.uni-hannover.de:123456789/1095
2022-12-02 17:14:09.708
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/10972022-12-02T16:16:27Zcom_123456789_1col_123456789_9doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Kaye, Jane
8996171a-0e4a-43c4-8c04-dbc69e453efb
600
Hurles, Matthew
69645ccb-0b62-4203-958b-6f7d11154581
600
Griffin, Heather
b55be510-0516-44c2-8883-4226ffefaf8b
600
Grewal, Jasjote
db072cdb-38ce-491f-8cf7-115311de0286
600
Bobrow, Martin
4eb73403-0b04-4e1d-884b-a2f2feb2abaa
600
Timpson, Nic
80de6e1b-3fb0-4f68-a458-5e0909ea4efa
600
Smee, Carol
bffcefd0-ce84-471a-bbda-6886a6b8cd58
600
Bolton, Patrick
442522d6-7e5c-439d-8d3d-248e6576eb8f
600
Durbin, Richard
0352eded-0dd7-46f2-bae8-fde5c4a68603
600
Dyke, Stephanie
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600
Fitzpatrick, David
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600
Kennedy, Karen
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600
Kent, Alastair
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600
Muddyman, Dawn
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600
Muntoni, Francesco
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600
Raymond, Lucy F.
fc188e06-f876-4725-8fc0-19ab8792e003
600
Semple, Robert
e6773980-1234-486e-bfe2-1d6d4a046733
600
Spector, Tim
f36a6ec3-077f-40b3-86c7-26b0232f4cc3
600
2017-01-27T08:36:56Z
2017-01-27T08:36:56Z
2014
Kaye, J.; Hurles, M.; Griffin, H.; Grewal, Jasote; Bobrow, M. et al.: Managing clinically significant findings in research: The UK10K example. In: European Journal of Human Genetics 22 (2014), Nr. 9, S. 1100-1104. DOI: https://doi.org/10.1038/ejhg.2013.290
http://www.repo.uni-hannover.de/handle/123456789/1097
http://dx.doi.org/10.15488/1073
Recent advances in sequencing technology allow data on the human genome to be generated more quickly and in greater detail than ever before. Such detail includes findings that may be of significance to the health of the research participant involved. Although research studies generally do not feed back information on clinically significant findings (CSFs) to participants, this stance is increasingly being questioned. There may be difficulties and risks in feeding clinically significant information back to research participants, however, the UK10K consortium sought to address these by creating a detailed management pathway. This was not intended to create any obligation upon the researchers to feed back any CSFs they discovered. Instead, it provides a mechanism to ensure that any such findings can be passed on to the participant where appropriate. This paper describes this mechanism and the specific criteria, which must be fulfilled in order for a finding and participant to qualify for feedback. This mechanism could be used by future research consortia, and may also assist in the development of sound principles for dealing with CSFs. © 2014 Macmillan Publishers Limited All rights reserved.
Made available in DSpace on 2017-01-27T08:36:56Z (GMT). No. of bitstreams: 0
Previous issue date: 2014
Wellcome Trust/WT091310
Wellcome Trust/WT096599/2/11/Z
publishedVersion
eng
London : Nature Publishing Group
European Journal of Human Genetics 22 (2014), Nr. 9
1018-4813
https://doi.org/10.1038/ejhg.2013.290
CC BY 3.0 Unported
https://creativecommons.org/licenses/by/3.0/
consortia
ethics
incidental findings
management pathway
research
sequencing
article
clinical genetics
clinical research
clinically significant finding
feedback system
gene sequence
genetic counseling
genetic variability
human
human genome
incidental finding
informed consent
interpersonal communication
lifespan
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Managing clinically significant findings in research: The UK10K example
Article
Text
9
22
1100
1104
openAccess
ORIGINAL
ejhg2013290a.pdf
ejhg2013290a.pdf
application/pdf
486304
https://www.repo.uni-hannover.de/bitstream/123456789/1097/1/ejhg2013290a.pdf
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MD5
1
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ejhg2013290a.pdf.txt
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text/plain
26877
https://www.repo.uni-hannover.de/bitstream/123456789/1097/2/ejhg2013290a.pdf.txt
cec54dceb29ba78e27e8d9448ac68fde
MD5
2
THUMBNAIL
ejhg2013290a.pdf.jpg
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https://www.repo.uni-hannover.de/bitstream/123456789/1097/3/ejhg2013290a.pdf.jpg
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MD5
3
123456789/1097
oai:www.repo.uni-hannover.de:123456789/1097
2022-12-02 17:16:27.074
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/10982022-12-02T16:10:14Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Wild, Birgit
4f63a351-d063-4871-966b-28e9d0b7a1c0
600
Schnecker, Jörg
bbdff247-031d-4761-be64-b301808b0a5b
600
Alves, Ricardo J. Eloy
caf43a3b-faa8-4dd6-b226-2ccfb72abe6b
600
Barsukov, Pavel
dee2b1f4-22b2-441e-a975-3a315fd3483e
600
Barta, Jiri
1fad0405-08f6-4f7a-a304-0077dcafcf30
600
Čapek, Petr
6be5e6f5-a185-48f9-b2f8-d7c591973c1a
600
Gentsch, Norman
60fa96f4-8f1e-4f7f-9d7b-cfd5518c5f39
600
Gittel, Antje
92a67e11-08b2-475a-a2f5-81e064201ce5
600
Guggenberger, Georg
32226bc2-d1b6-49d7-bae6-fda855a5f5d4
600
Lashchinskiy, Nikolay
f5086172-b268-4f81-8097-673f3e890ae1
600
Mikutta, Robert
011194d0-939e-421b-bd3d-cdf0034e53d9
600
Rusalimova, Olga
21cd0fe7-024b-4222-ab2c-c3dd0be457f3
600
Šantrůčková, Hana
2ed723f8-fdb2-4d46-891c-99dc5e4401e6
600
Shibistova, Olga
15b92bb6-8e1a-4c95-bbb7-52355b5e44a6
600
Urich, Tim
4a5959b2-6d53-4737-929c-5fca617fe751
600
Watzka, Margarete
89c158c9-5b99-4633-bca5-3ba9394a2d23
600
Zrazhevskaya, Galina
c3b6ee34-b0b4-412f-96bf-9797403651d7
600
Richter, Andreas
52d71f9c-5e93-47ae-9872-02fab3c97c0c
600
2017-01-27T08:36:56Z
2017-01-27T08:36:56Z
2014
Wild, B.; Schnecker, J.; Alves, R.J.E.; Barsukov, P.; Bárta, Jiri et al.: Input of easily available organic C and N stimulates microbial decomposition of soil organic matter in arctic permafrost soil. In: Soil Biology and Biochemistry 75 (2014), S. 143-151. DOI: https://doi.org/10.1016/j.soilbio.2014.04.014
http://www.repo.uni-hannover.de/handle/123456789/1098
http://dx.doi.org/10.15488/1074
Rising temperatures in the Arctic can affect soil organic matter (SOM) decomposition directly and indirectly, by increasing plant primary production and thus the allocation of plant-derived organic compounds into the soil. Such compounds, for example root exudates or decaying fine roots, are easily available for microorganisms, and can alter the decomposition of older SOM ("priming effect"). We here report on a SOM priming experiment in the active layer of a permafrost soil from the central Siberian Arctic, comparing responses of organic topsoil, mineral subsoil, and cryoturbated subsoil material (i.e., poorly decomposed topsoil material subducted into the subsoil by freeze-thaw processes) to additions of 13C-labeled glucose, cellulose, a mixture of amino acids, and protein (added at levels corresponding to approximately 1% of soil organic carbon). SOM decomposition in the topsoil was barely affected by higher availability of organic compounds, whereas SOM decomposition in both subsoil horizons responded strongly. In the mineral subsoil, SOM decomposition increased by a factor of two to three after any substrate addition (glucose, cellulose, amino acids, protein), suggesting that the microbial decomposer community was limited in energy to break down more complex components of SOM. In the cryoturbated horizon, SOM decomposition increased by a factor of two after addition of amino acids or protein, but was not significantly affected by glucose or cellulose, indicating nitrogen rather than energy limitation. Since the stimulation of SOM decomposition in cryoturbated material was not connected to microbial growth or to a change in microbial community composition, the additional nitrogen was likely invested in the production of extracellular enzymes required for SOM decomposition. Our findings provide a first mechanistic understanding of priming in permafrost soils and suggest that an increase in the availability of organic carbon or nitrogen, e.g., by increased plant productivity, can change the decomposition of SOM stored in deeper layers of permafrost soils, with possible repercussions on the global climate.
Made available in DSpace on 2017-01-27T08:36:56Z (GMT). No. of bitstreams: 0
Previous issue date: 2014
Austrian Science Fund (FWF)/CryoCARB
publishedVersion
eng
London : Elsevier Ltd.
Soil Biology and Biochemistry 75 (2014)
0038-0717
https://doi.org/10.1016/j.soilbio.2014.04.014
CC BY 3.0 Unported
https://creativecommons.org/licenses/by/3.0/
Organic matter decomposition
Permafrost
Phospholipid fatty acid (PLFA)
Priming
Tundra
Amino acids
Biogeochemistry
Cellulose
Climate change
Glucose
Microorganisms
Nitrogen
Organic compounds
Permafrost
Phospholipids
Proteins
Decomposer communities
Extracellular enzymes
Microbial community composition
Microbial decomposition
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Input of easily available organic C and N stimulates microbial decomposition of soil organic matter in arctic permafrost soil
Article
Text
75
143
151
openAccess
ORIGINAL
1-s2.0-S0038071714001345-main.pdf
1-s2.0-S0038071714001345-main.pdf
application/pdf
663586
https://www.repo.uni-hannover.de/bitstream/123456789/1098/1/1-s2.0-S0038071714001345-main.pdf
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MD5
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1-s2.0-S0038071714001345-main.pdf.txt
1-s2.0-S0038071714001345-main.pdf.txt
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47707
https://www.repo.uni-hannover.de/bitstream/123456789/1098/2/1-s2.0-S0038071714001345-main.pdf.txt
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MD5
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MD5
3
123456789/1098
oai:www.repo.uni-hannover.de:123456789/1098
2022-12-02 17:10:14.474
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/11682022-12-02T16:11:41Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570ddc:500
Berthoud, Viviana M.
42b1cd67-fc5d-48b0-a8a2-f3bf3009186a
600
Ngezahayo, Anaclet
98e403d3-05d2-4c08-a36f-2a82d0a12bc6
600
2017-02-07T11:12:10Z
2017-02-07T11:12:10Z
2017
Berthoud, Viviana M.; Ngezahayo, Anaclet: Focus on lens connexins. In: BMC Cell Biology 18 (2017), Suppl. 1, 6. DOI: https://doi.org/10.1186/s12860-016-0116-6
http://www.repo.uni-hannover.de/handle/123456789/1168
http://dx.doi.org/10.15488/1144
The lens is an avascular organ composed of an anterior epithelial cell layer and fiber cells that form the bulk of the organ. The lens expresses connexin43 (Cx43), connexin46 (Cx46) and connexin50 (Cx50). Epithelial Cx50 has critical roles in cell proliferation and differentiation, likely involving growth factor-dependent signaling pathways. Both Cx46 and Cx50 are crucial for lens transparency; mutations in their genes have been linked to congenital and age-related cataracts. Congenital cataract-associated connexin mutants can affect protein trafficking, stability and/or function, and the functional effects may differ between gap junction channels and hemichannels. Dominantly inherited cataracts may result from effects of the connexin mutant on its wild type isotype, the other co-expressed wild type connexin and/or its interaction with other cellular components.
Made available in DSpace on 2017-02-07T11:12:10Z (GMT). No. of bitstreams: 0
Previous issue date: 2017
NIH/RO1EY08368
DFG/SFB/Transregio37
US National Institutes of Health/RO1EY08368
DFG/SFB/Transregio 37
publishedVersion
eng
London : Biomed Central
BMC Cell Biology 18 (2017), Suppl. 1
1471-2121
https://doi.org/10.1186/s12860-016-0116-6
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
ocular lens
Ubiquitylation
Phosphorylation
Lens connexin function
Congenital cataracts
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Focus on lens connexins
Article
Text
Suppl. 1
18
6
openAccess
LUH_Fonds
ORIGINAL
art_10.1186_s12860-016-0116-6.pdf
art_10.1186_s12860-016-0116-6.pdf
application/pdf
1002355
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MD5
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TEXT
art_10.1186_s12860-016-0116-6.pdf.txt
art_10.1186_s12860-016-0116-6.pdf.txt
Extracted Text
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57699
https://www.repo.uni-hannover.de/bitstream/123456789/1168/2/art_10.1186_s12860-016-0116-6.pdf.txt
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MD5
2
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MD5
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123456789/1168
oai:www.repo.uni-hannover.de:123456789/1168
2022-12-02 17:11:41.023
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/11722022-12-02T15:19:59Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Fondevilla, Sara
2b153e63-dbea-4a80-922a-f9064e262319
600
Küster, Helge
6fa7584e-ec79-44a9-ba44-9598749ab6dd
600
Krajinski, Franziska
9e60cb19-8df8-4e24-91eb-76ecedb3b903
600
Cubero, José I.
c734375c-e69c-4333-85e4-d32b2b35130a
600
Rubiales, Diego
1677fe8b-f26a-45aa-ae70-0818ba0ca234
600
2017-02-23T13:12:47Z
2017-02-23T13:12:47Z
2011
Fondevilla, S.; Küster, Helge; Krajinski, F.; Cubero, J.I.; Rubiales, D.: Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology. In: BMC Genomics 12 (2011), 28. DOI: https://doi.org/10.1186/1471-2164-12-28
http://www.repo.uni-hannover.de/handle/123456789/1172
http://dx.doi.org/10.15488/1148
Background: Ascochyta blight, caused by Mycosphaerella pinodes is one of the most important pea pathogens. However, little is known about the genes and mechanisms of resistance acting against M. pinodes in pea. Resistance identified so far to this pathogen is incomplete, polygenic and scarce in pea, being most common in Pisum relatives. The identification of the genes underlying resistance would increase our knowledge about M. pinodes-pea interaction and would facilitate the introgression of resistance into pea varieties. In the present study differentially expressed genes in the resistant P. sativum ssp. syriacum accession P665 comparing to the susceptible pea cv. Messire after inoculation with M. pinodes have been identified using a M. truncatula microarray.Results: Of the 16,470 sequences analysed, 346 were differentially regulated. Differentially regulated genes belonged to almost all functional categories and included genes involved in defense such as genes involved in cell wall reinforcement, phenylpropanoid and phytoalexins metabolism, pathogenesis- related (PR) proteins and detoxification processes. Genes associated with jasmonic acid (JA) and ethylene signal transduction pathways were induced suggesting that the response to M. pinodes in pea is regulated via JA and ET pathways. Expression levels of ten differentially regulated genes were validated in inoculated and control plants using qRT-PCR showing that the P665 accession shows constitutively an increased expression of the defense related genes as peroxidases, disease resistance response protein 39 (DRR230-b), glutathione S-transferase (GST) and 6a-hydroxymaackiain methyltransferase.Conclusions: Through this study a global view of genes expressed during resistance to M. pinodes has been obtained, giving relevant information about the mechanisms and pathways conferring resistance to this important disease. In addition, the M. truncatula microarray represents an efficient tool to identify candidate genes controlling resistance to M. pinodes in pea.
Made available in DSpace on 2017-02-23T13:12:47Z (GMT). No. of bitstreams: 0
Previous issue date: 2011
Spanish Ministry of Science and Innovation
AGL2008-01239
FP6-CT-2004-FOOD-1-506223
publishedVersion
eng
London : BioMed Central Ltd.
BMC Genomics 12 (2011)
1471-2164
https://doi.org/10.1186/1471-2164-12-28
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
glutathione transferase
peroxidase
article
Ascomycetes
cell wall
controlled study
gene expression
gene expression regulation
gene identification
genetic association
genetic resistance
genetic susceptibility
membrane transport
microarray analysis
Mycosphaerella pinodes
nonhuman
nucleotide sequence
protein binding
protein metabolism
quantitative analysis
reverse transcription polymerase chain reaction
signal transduction
gene expression profiling
genetics
immunology
innate immunity
methodology
microbiology
pea
physiology
plant disease
Ascochyta
Didymella pinodes
Pisum
Pisum sativum
Ascomycota
Gene Expression Profiling
Immunity, Innate
Microarray Analysis
Peas
Plant Diseases
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology
Article
Text
12
28
openAccess
ORIGINAL
art_10.1186_1471-2164-12-28.pdf
art_10.1186_1471-2164-12-28.pdf
application/pdf
504671
https://www.repo.uni-hannover.de/bitstream/123456789/1172/1/art_10.1186_1471-2164-12-28.pdf
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MD5
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art_10.1186_1471-2164-12-28.pdf.txt
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75180
https://www.repo.uni-hannover.de/bitstream/123456789/1172/2/art_10.1186_1471-2164-12-28.pdf.txt
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MD5
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MD5
3
123456789/1172
oai:www.repo.uni-hannover.de:123456789/1172
2022-12-02 16:19:59.015
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/11852022-12-02T16:11:41Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Haar, Marcel von der
a2ddce4f-8606-46c2-87a4-c6d54f4eaf1b
600
Heuer, Christopher
782541de-c71d-4095-960e-54d7e56c97de
600
Pähler, Martin
69f69a2e-afb7-4573-a386-7eaf7184038b
600
Haar, Kathrin von der
9704a148-a5f0-4d68-872f-dc2d5681cc76
600
Lindner, Patrick
0a939ab9-a11e-4be5-85c0-4c9a9daa54b2
600
Scheper, Thomas
05a38e4d-93b1-43ab-8ffc-43e89bb8a642
600
Stahl, Frank
9e2ff2ed-280d-46d6-a902-35bd40adfecd
600
2017-02-24T08:49:27Z
2017-02-24T08:49:27Z
2016
von der Haar, Marcel; Heuer, Christopher; Pähler, Martin; von der Haar, Kathrin; Lindner, Patrick et al.: Optimization of cyanine dye stability and analysis of FRET interaction on DNA microarrays. In: Biology 5 (2016), Nr. 4, 47. DOI: https://doi.org/10.3390/biology5040047
http://www.repo.uni-hannover.de/handle/123456789/1185
http://dx.doi.org/10.15488/1161
The application of DNA microarrays for high throughput analysis of genetic regulation is often limited by the fluorophores used as markers. The implementation of multi-scan techniques is limited by the fluorophores’ susceptibility to photobleaching when exposed to the scanner laser light. This paper presents combined mechanical and chemical strategies which enhance the photostability of cyanine 3 and cyanine 5 as part of solid state DNA microarrays. These strategies are based on scanning the microarrays while the hybridized DNA is still in an aqueous solution with the presence of a reductive/oxidative system (ROXS). Furthermore, the experimental setup allows for the analysis and eventual normalization of Förster-resonance-energy-transfer (FRET) interaction of cyanine-3/cyanine-5 dye combinations on the microarray. These findings constitute a step towards standardization of microarray experiments and analysis and may help to increase the comparability of microarray experiment results between labs.
Made available in DSpace on 2017-02-24T08:49:27Z (GMT). No. of bitstreams: 0
Previous issue date: 2016
publishedVersion
eng
Basel : MDPI AG
Biology 5 (2016), Nr. 4
2079-7737
https://doi.org/10.3390/biology5040047
CC BY-NC-SA 4.0 Unported
https://creativecommons.org/licenses/by-nc-sa/4.0/
Bioanalytics
Bioinformatics
Cyanine dye
DNA
Fluorophore
FRET
Microarray
Photobleaching
ROXS
Scanning
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Optimization of cyanine dye stability and analysis of FRET interaction on DNA microarrays
Article
Text
4
5
47
openAccess
ORIGINAL
biology-05-00047.pdf
biology-05-00047.pdf
application/pdf
2949968
https://www.repo.uni-hannover.de/bitstream/123456789/1185/1/biology-05-00047.pdf
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MD5
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MD5
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https://www.repo.uni-hannover.de/bitstream/123456789/1185/3/biology-05-00047.pdf.jpg
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MD5
3
123456789/1185
oai:www.repo.uni-hannover.de:123456789/1185
2022-12-02 17:11:41.871
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/12082022-12-02T16:17:36Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Vorhölter, Frank-Jörg
60f1e53b-61e3-46e8-8360-2453c7891026
600
Wiggerich, Heinrich-Günter
74382396-0886-4a79-b2ab-826366d1bbfa
600
Scheidle, Heiko
97a04d83-9518-4a7d-a408-ba3646191118
600
Sidhu, Vishaldeep Kaur
5e0fdb3b-f821-4a20-a99d-10c7cb5b1d63
600
Mrozek, Kalina
5e537ce1-b874-4ce1-b4a0-680f746a7959
600
Küster, Helge
6fa7584e-ec79-44a9-ba44-9598749ab6dd
600
Pühler, Alfred
80e78991-dd6a-4904-a247-52415878f11a
600
Niehaus, Karsten
6d34fc77-e3b0-477e-af98-8ea2f93c6d0e
600
2017-03-02T14:05:10Z
2017-03-02T14:05:10Z
2012
Vorhoelter, Frank-Joerg; Wiggerich, Heinrich-Guenter; Scheidle, Heiko; Sidhu, Vishaldeep Kaur; Mrozek, Kalina et al.: Involvement of bacterial TonB-dependent signaling in the generation of an oligogalacturonide damage-associated molecular pattern from plant cell walls exposed to Xanthomonas campestris pv. campestris pectate lyases. In: BMC Microbiology 12 (2012), 239. DOI: https://doi.org/10.1186/1471-2180-12-239
http://www.repo.uni-hannover.de/handle/123456789/1208
http://dx.doi.org/10.15488/1184
Background: Efficient perception of attacking pathogens is essential for plants. Plant defense is evoked by molecules termed elicitors. Endogenous elicitors or damage-associated molecular patterns (DAMPs) originate from plant materials upon injury or pathogen activity. While there are comparably well-characterized examples for DAMPs, often oligogalacturonides (OGAs), generated by the activity of fungal pathogens, endogenous elicitors evoked by bacterial pathogens have been rarely described. In particular, the signal perception and transduction processes involved in DAMP generation are poorly characterized. Results: A mutant strain of the phytopathogenic bacterium Xanthomonas campestris pv. campestris deficient in exbD2, which encodes a component of its unusual elaborate TonB system, had impaired pectate lyase activity and caused no visible symptoms for defense on the non-host plant pepper (Capsicum annuum). A co-incubation of X. campestris pv. campestris with isolated cell wall material from C. annuum led to the release of compounds which induced an oxidative burst in cell suspension cultures of the non-host plant. Lipopolysaccharides and proteins were ruled out as elicitors by polymyxin B and heat treatment, respectively. After hydrolysis with trifluoroacetic acid and subsequent HPAE chromatography, the elicitor preparation contained galacturonic acid, the monosaccharide constituent of pectate. OGAs were isolated from this crude elicitor preparation by HPAEC and tested for their biological activity. While small OGAs were unable to induce an oxidative burst, the elicitor activity in cell suspension cultures of the non-host plants tobacco and pepper increased with the degree of polymerization (DP). Maximal elicitor activity was observed for DPs exceeding 8. In contrast to the X. campestris pv. campestris wild type B100, the exbD2 mutant was unable to generate elicitor activity from plant cell wall material or from pectin. Conclusions: To our knowledge, this is the second report on a DAMP generated by bacterial features. The generation of the OGA elicitor is embedded in a complex exchange of signals within the framework of the plant-microbe interaction of C. annuum and X. campestris pv. campestris. The bacterial TonB-system is essential for the substrate-induced generation of extracellular pectate lyase activity. This is the first demonstration that a TonB-system is involved in bacterial trans-envelope signaling in the context of a pathogenic interaction with a plant.
Made available in DSpace on 2017-03-02T14:05:10Z (GMT). No. of bitstreams: 0
Previous issue date: 2012
BMBF/GenoMik Plus
publishedVersion
eng
London : BioMed Central Ltd
BMC Microbiology 12 (2012)
1471-2180
https://doi.org/10.1186/1471-2180-12-239
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
TonB system
Damage-associate molecular pattern
DAMP
Oligogalacturonide
Trans-envelope signaling
Molecular plant-microbe interaction
Pathogen
Xanthomonas campestris
host-pathogen interactions
elicits phytoalexin accumulation
complete genome sequence
f-sp glycinea
hypersensitive response
innate immunity
disease resistance
oxidative burst
polygalacturonate lyase
transduction systems
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Involvement of bacterial TonB-dependent signaling in the generation of an oligogalacturonide damage-associated molecular pattern from plant cell walls exposed to Xanthomonas campestris pv. campestris pectate lyases
Article
Text
12
239
openAccess
ORIGINAL
art_10.1186_1471-2180-12-239.pdf
art_10.1186_1471-2180-12-239.pdf
application/pdf
1034042
https://www.repo.uni-hannover.de/bitstream/123456789/1208/1/art_10.1186_1471-2180-12-239.pdf
fc172e3d45a87b3ca19277ebc5b78f42
MD5
1
TEXT
art_10.1186_1471-2180-12-239.pdf.txt
art_10.1186_1471-2180-12-239.pdf.txt
Extracted Text
text/plain
95997
https://www.repo.uni-hannover.de/bitstream/123456789/1208/2/art_10.1186_1471-2180-12-239.pdf.txt
bb816f7c1281da358328324dff51e7b1
MD5
2
THUMBNAIL
art_10.1186_1471-2180-12-239.pdf.jpg
art_10.1186_1471-2180-12-239.pdf.jpg
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1724
https://www.repo.uni-hannover.de/bitstream/123456789/1208/3/art_10.1186_1471-2180-12-239.pdf.jpg
72e8e0d6583562b180f29443a33c66b3
MD5
3
123456789/1208
oai:www.repo.uni-hannover.de:123456789/1208
2022-12-02 17:17:36.834
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/12102022-12-02T16:17:36Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Horst, Ina
44f0f7ee-5288-490b-b1ce-7083a3e398d7
600
Offermann, Sascha
c6212709-b231-4b1d-b179-e3aabb501a5f
600
Dreesen, Bjoern
af1d4706-2673-4589-b490-cde8554fa3b0
600
Niessen, Markus
d5978a1d-93a6-4473-b8a4-d12d47eb18e6
600
Peterhansel, Christoph
d9e74e8e-25d2-48d1-872f-0c488ef78319
600
2017-03-02T14:05:12Z
2017-03-02T14:05:12Z
2009
Horst, Ina; Offermann, Sascha; Dreesen, Bjoern; Niessen, Markus; Peterhansel, Christoph: Core promoter acetylation is not required for high transcription from the phosphoenolpyruvate carboxylase promoter in maize. In: Epigenetics & Chromatin 2 (2009), 17. DOI: https://doi.org/10.1186/1756-8935-2-17
http://www.repo.uni-hannover.de/handle/123456789/1210
http://dx.doi.org/10.15488/1186
Background: Acetylation of promoter nucleosomes is tightly correlated and mechanistically linked to gene activity. However, transcription is not necessary for promoter acetylation. It seems, therefore, that external and endogenous stimuli control histone acetylation and by this contribute to gene regulation. Photosynthetic genes in plants are excellent models with which to study the connection between stimuli and chromatin modifications because these genes are strongly expressed and regulated by multiple stimuli that are easily manipulated. We have previously shown that acetylation of specific histone lysine residues on the photosynthetic phosphoenolpyruvate carboxylase (Pepc) promoter in maize is controlled by light and is independent of other stimuli or gene activity. Acetylation of upstream promoter regions responds to a set of other stimuli which include the nutrient availability of the plant. Here, we have extended these studies by analysing histone acetylation during the diurnal and circadian rhythm of the plant. Results: We show that histone acetylation of individual lysine residues is removed from the core promoter before the end of the illumination period which is an indication that light is not the only factor influencing core promoter acetylation. Deacetylation is accompanied by a decrease in gene activity. Pharmacological inhibition of histone deacetylation is not sufficient to prevent transcriptional repression, indicating that deacetylation is not controlling diurnal gene regulation. Variation of the Pepc promoter activity during the day is controlled by the circadian oscillator as it is maintained under constant illumination for at least 3 days. During this period, light-induced changes in histone acetylation are completely removed from the core promoter, although the light stimulus is continuously applied. However, acetylation of most sites on upstream promoter elements follows the circadian rhythm. Conclusion: Our results suggest a central role of upstream promoter acetylation in the quantitative regulation of gene expression in this model gene. Induced core promoter acetylation is dispensable for the highest gene expression in the diurnal and circadian rhythm.
Made available in DSpace on 2017-03-02T14:05:12Z (GMT). No. of bitstreams: 0
Previous issue date: 2009
DFG
publishedVersion
eng
London : BioMed Central Ltd
Epigenetics & Chromatin 2 (2009)
1756-8935
https://doi.org/10.1186/1756-8935-2-17
CC BY 2.0 Unported
https://creativecommons.org/licenses/by/2.0/
histone acetylation
gene-expression
trichostatin-a
chromatin
arabidopsis
methylation
reveals
clock
code
cells
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Core promoter acetylation is not required for high transcription from the phosphoenolpyruvate carboxylase promoter in maize
Article
Text
2
17
openAccess
ORIGINAL
art_10.1186_1756-8935-2-17.pdf
art_10.1186_1756-8935-2-17.pdf
application/pdf
652139
https://www.repo.uni-hannover.de/bitstream/123456789/1210/1/art_10.1186_1756-8935-2-17.pdf
82c52fa232dba664c42218c6addc0dcf
MD5
1
TEXT
art_10.1186_1756-8935-2-17.pdf.txt
art_10.1186_1756-8935-2-17.pdf.txt
Extracted Text
text/plain
46616
https://www.repo.uni-hannover.de/bitstream/123456789/1210/2/art_10.1186_1756-8935-2-17.pdf.txt
d458e6536531a29acc6443e1ff2a80eb
MD5
2
THUMBNAIL
art_10.1186_1756-8935-2-17.pdf.jpg
art_10.1186_1756-8935-2-17.pdf.jpg
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image/jpeg
1763
https://www.repo.uni-hannover.de/bitstream/123456789/1210/3/art_10.1186_1756-8935-2-17.pdf.jpg
8fdbf60e22931ded07870e3d757a2c89
MD5
3
123456789/1210
oai:www.repo.uni-hannover.de:123456789/1210
2022-12-02 17:17:36.846
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/12352022-12-02T16:17:36Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessddc:580ddc:570ddc:500
Benevenuto, Rafael Fonseca
c2ffcb2d-cefc-413d-b551-bf79d365ec5b
600
Agapito-Tenfen, Sarah Zanon
0a042c61-87e9-4816-90e7-c37742a2678b
600
Vilperte, Vinicius
e948b321-1758-4261-92b9-f8c6d9f6e27c
600
Wikmark, Odd-Gunnar
97a18fcf-ff14-4b4a-9a41-bbfb213f707c
600
van Rensburg, Peet Jansen
e43b87ed-e201-4a1e-805c-037150254ead
600
Nodari, Rubens Onofre
4212948e-80f8-442a-98d1-4f867d7e7e96
600
2017-03-17T10:51:57Z
2017-03-17T10:51:57Z
2017
Benevenuto, R.F.; Agapito-Tenfen, S.Z.; Vilperte, V.; Wikmark, O.-G.; van Rensburg, P.J.; Nodari, R.O.: Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses. In: PLoS ONE 12 (2017), Nr. 2, e0173069. DOI: https://doi.org/10.1371/journal.pone.0173069
http://www.repo.uni-hannover.de/handle/123456789/1235
http://dx.doi.org/10.15488/1211
Some genetically modified (GM) plants have transgenes that confer tolerance to abiotic stressors. Meanwhile, other transgenes may interact with abiotic stressors, causing pleiotropic effects that will affect the plant physiology. Thus, physiological alteration might have an impact on the product safety. However, routine risk assessment (RA) analyses do not evaluate the response of GM plants exposed to different environmental conditions. Therefore, we here present a proteome profile of herbicide-tolerant maize, including the levels of phytohormones and related compounds, compared to its near-isogenic non-GM variety under drought and herbicide stresses. Twenty differentially abundant proteins were detected between GM and non-GM hybrids under different water deficiency conditions and herbicide sprays. Pathway enrichment analysis showed that most of these proteins are assigned to energetic/carbohydrate metabolic processes. Among phytohormones and related compounds, different levels of ABA, CA, JA, MeJA and SA were detected in the maize varieties and stress conditions analysed. In pathway and proteome analyses, environment was found to be the major source of variation followed by the genetic transformation factor. Nonetheless, differences were detected in the levels of JA, MeJA and CA and in the abundance of 11 proteins when comparing the GM plant and its non-GM near-isogenic variety under the same environmental conditions. Thus, these findings do support molecular studies in GM plants Risk Assessment analyses.
Made available in DSpace on 2017-03-17T10:51:57Z (GMT). No. of bitstreams: 0
Previous issue date: 2017
Norwegian Agency for Development Cooperation
eng
San Francisco, CA : Public Library of Science
PLoS ONE 12 (2017), Nr. 2
1932-6203
https://doi.org/10.1371/journal.pone.0173069
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Dewey Decimal Classification::500 | Naturwissenschaften
500
600
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
580
600
Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses
Article
Text
2
12
e0173069
openAccess
ORIGINAL
Benevenuto et al 2017, CC, Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses.pdf
Benevenuto et al 2017, CC, Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses.pdf
application/pdf
977956
https://www.repo.uni-hannover.de/bitstream/123456789/1235/1/Benevenuto%20et%20al%202017%2c%20CC%2c%20Molecular%20responses%20of%20genetically%20modified%20maize%20to%20abiotic%20stresses%20as%20determined%20through%20proteomic%20and%20metabolomic%20analyses.pdf
b2ec664f11c1baee1e9c307a4feda46a
MD5
1
TEXT
Benevenuto et al 2017, CC, Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses.pdf.txt
Benevenuto et al 2017, CC, Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses.pdf.txt
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91690
https://www.repo.uni-hannover.de/bitstream/123456789/1235/2/Benevenuto%20et%20al%202017%2c%20CC%2c%20Molecular%20responses%20of%20genetically%20modified%20maize%20to%20abiotic%20stresses%20as%20determined%20through%20proteomic%20and%20metabolomic%20analyses.pdf.txt
e1a8ee3a8b96a12a57757d931207cb11
MD5
2
THUMBNAIL
Benevenuto et al 2017, CC, Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses.pdf.jpg
Benevenuto et al 2017, CC, Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses.pdf.jpg
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https://www.repo.uni-hannover.de/bitstream/123456789/1235/3/Benevenuto%20et%20al%202017%2c%20CC%2c%20Molecular%20responses%20of%20genetically%20modified%20maize%20to%20abiotic%20stresses%20as%20determined%20through%20proteomic%20and%20metabolomic%20analyses.pdf.jpg
a51814320e386e51947282092cea763e
MD5
3
123456789/1235
oai:www.repo.uni-hannover.de:123456789/1235
2022-12-02 17:17:36.873
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/12782022-12-02T16:14:10Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:610ddc:570
Volohonsky, Gloria
6c83f09d-936a-4f07-942c-92c5056c70ba
600
Hopp, Ann-Katrin
22b24ec9-0f2b-43e0-ba2e-b0a7cbf54960
600
Saenger, Melanie
ff0785b0-397d-4df3-b242-894bbb0cb279
600
Soichot, Julien
430f2bac-f5ba-403f-8e52-cb0a99050a6e
600
Scholze, Heidi
865aaf18-6560-4516-a1fb-ac292d7dff77
600
Boch, Jens
6de0e533-6052-4a91-856f-eacad46f6cd4
600
Blandin, Stephanie A.
97c6673a-cfd0-4230-b21d-791b7c512a50
600
Marois, Eric
e0c21518-aa9d-4c6d-9d23-23a051738b2d
600
2017-03-31T08:16:08Z
2017-03-31T08:16:08Z
2017
Volohonsky, G.; Hopp, A.-K.; Saenger, M.; Soichot, J.; Scholze, H. et al.: Transgenic Expression of the Anti-parasitic Factor TEP1 in the Malaria Mosquito Anopheles gambiae. In: PLoS Pathogens 13 (2017), Nr. 1, e1006113. DOI: https://doi.org/10.1371/journal.ppat.1006113
http://www.repo.uni-hannover.de/handle/123456789/1278
http://dx.doi.org/10.15488/1253
Mosquitoes genetically engineered to be resistant to Plasmodium parasites represent a promising novel approach in the fight against malaria. The insect immune system itself is a source of anti-parasitic genes potentially exploitable for transgenic designs. The Anopheles gambiae thioester containing protein 1 (TEP1) is a potent anti-parasitic protein. TEP1 is secreted and circulates in the mosquito hemolymph, where its activated cleaved form binds and eliminates malaria parasites. Here we investigated whether TEP1 can be used to create malaria resistant mosquitoes. Using a GFP reporter transgene, we determined that the fat body is the main site of TEP1 expression. We generated transgenic mosquitoes that express TEP1r, a potent refractory allele of TEP1, in the fat body and examined the activity of the transgenic protein in wild-type or TEP1 mutant genetic backgrounds. Transgenic TEP1r rescued loss-of-function mutations, but did not increase parasite resistance in the presence of a wild-type susceptible allele. Consistent with previous reports, TEP1 protein expressed from the transgene in the fat body was taken up by hemocytes upon a challenge with injected bacteria. Furthermore, although maturation of transgenic TEP1 into the cleaved form was impaired in one of the TEP1 mutant lines, it was still sufficient to reduce parasite numbers and induce parasite melanization. We also report here the first use of Transcription Activator Like Effectors (TALEs) in Anopheles gambiae to stimulate expression of endogenous TEP1. We found that artificial elevation of TEP1 expression remains moderate in vivo and that enhancement of endogenous TEP1 expression did not result in increased resistance to Plasmodium. Taken together, our results reveal the difficulty of artificially influencing TEP1-mediated Plasmodium resistance, and contribute to further our understanding of the molecular mechanisms underlying mosquito resistance to Plasmodium parasites.
Made available in DSpace on 2017-03-31T08:16:08Z (GMT). No. of bitstreams: 0
Previous issue date: 2017
publishedVersion
eng
San Francisco, CA : Public Library of Science
PLoS Pathogens 13 (2017), Nr. 1
1553-7366
https://doi.org/10.1371/journal.ppat.1006113
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
fats
blood
larvae
mosquitoes
parasitic diseases
Plasmodium
hemocytes
luciferase
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
610
600
Transgenic Expression of the Anti-parasitic Factor TEP1 in the Malaria Mosquito Anopheles gambiae
Article
Text
1
13
e1006113
openAccess
ORIGINAL
journal.ppat.1006113.pdf
journal.ppat.1006113.pdf
application/pdf
2426150
https://www.repo.uni-hannover.de/bitstream/123456789/1278/1/journal.ppat.1006113.pdf
aef3c1cb926cf88ca36d2016bcdeeaa8
MD5
1
TEXT
journal.ppat.1006113.pdf.txt
journal.ppat.1006113.pdf.txt
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text/plain
88972
https://www.repo.uni-hannover.de/bitstream/123456789/1278/2/journal.ppat.1006113.pdf.txt
7165c6445de7c2a5f1152c194fd15f43
MD5
2
THUMBNAIL
journal.ppat.1006113.pdf.jpg
journal.ppat.1006113.pdf.jpg
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1734
https://www.repo.uni-hannover.de/bitstream/123456789/1278/3/journal.ppat.1006113.pdf.jpg
94b965b47f1f6007bbb045df9d408dc3
MD5
3
123456789/1278
oai:www.repo.uni-hannover.de:123456789/1278
2022-12-02 17:14:10.553
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/12952022-12-02T16:17:36Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:580ddc:570
Streubel, Jana
a6da8321-f306-45ce-848c-9c46f6979822
600
Baum, Heidi
a6c3c84e-8a82-4f4c-8fcb-6262e58ac004
600
Grau, Jan
c688ae96-d3ce-4d60-bd8c-e97a9246df9d
600
Stuttman, Johannes
1a858b55-9212-48aa-b089-ce38fc11841c
600
Boch, Jens
6de0e533-6052-4a91-856f-eacad46f6cd4
600
2017-04-06T06:44:26Z
2017-04-06T06:44:26Z
2017
Streubel, J.; Baum, H.; Grau, J.; Stuttman, J.; Boch, J.: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. In: PLoS ONE 12 (2017), Nr. 3, e0173580. DOI: https://doi.org/10.1371/journal.pone.0173580
http://www.repo.uni-hannover.de/handle/123456789/1295
http://dx.doi.org/10.15488/1270
Plant-pathogenic Xanthomonas bacteria inject transcription activator-like effector proteins (TALEs) into host cells to specifically induce transcription of plant genes and enhance susceptibility. Although the DNA-binding mode is well-understood it is still ambiguous how TALEs initiate transcription and whether additional promoter elements are needed to support this. To systematically dissect prerequisites for transcriptional initiation the activity of one TALE was compared on different synthetic Bs4 promoter fragments. In addition, a large collection of artificial TALEs spanning the OsSWEET14 promoter was compared. We show that the presence of a TALE alone is not sufficient to initiate transcription suggesting the requirement of additional supporting promoter elements. At the OsSWEET14 promoter TALEs can initiate transcription from various positions, in a synergistic manner of multiple TALEs binding in parallel to the promoter, and even by binding in reverse orientation. TALEs are known to shift the transcriptional start site, but our data show that this shift depends on the individual position of a TALE within a promoter context. Our results implicate that TALEs function like classical enhancer-binding proteins and initiate transcription in both orientations which has consequences for in planta target gene prediction and design of artificial activators.
Made available in DSpace on 2017-04-06T06:44:26Z (GMT). No. of bitstreams: 0
Previous issue date: 2017
DFG/BO1496/7-1
DFG/BO1496/8-1
DFG/GR4587/1-1
COST/FA/FA1208
publishedVersion
eng
San Francisco, CA : Public Library of Science
PLoS ONE 12 (2017), Nr. 3
1932-6203
https://doi.org/10.1371/journal.pone.0173580
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
570
600
Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
580
600
Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations
Article
Text
3
12
e0173580
openAccess
ORIGINAL
Streubel et al 2017, CC, Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations.pdf
Streubel et al 2017, CC, Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations.pdf
application/pdf
1802391
https://www.repo.uni-hannover.de/bitstream/123456789/1295/1/Streubel%20et%20al%202017%2c%20CC%2c%20Dissection%20of%20TALE-dependent%20gene%20activation%20reveals%20that%20they%20induce%20transcription%20cooperatively%20and%20in%20both%20orientations.pdf
a8942b6d116a89254c76df6870b37436
MD5
1
TEXT
Streubel et al 2017, CC, Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations.pdf.txt
Streubel et al 2017, CC, Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations.pdf.txt
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85266
https://www.repo.uni-hannover.de/bitstream/123456789/1295/2/Streubel%20et%20al%202017%2c%20CC%2c%20Dissection%20of%20TALE-dependent%20gene%20activation%20reveals%20that%20they%20induce%20transcription%20cooperatively%20and%20in%20both%20orientations.pdf.txt
21023dad8d85a6afcfb2b95e7c1b0e91
MD5
2
THUMBNAIL
Streubel et al 2017, CC, Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations.pdf.jpg
Streubel et al 2017, CC, Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations.pdf.jpg
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752565e01e909db11c6ee2dc803c395e
MD5
3
123456789/1295
oai:www.repo.uni-hannover.de:123456789/1295
2022-12-02 17:17:36.88
Institutionelles Repositorium der Leibniz Universität Hannover
dspace@admin.tib.eu
oai:www.repo.uni-hannover.de:123456789/12962022-12-02T16:17:36Zcom_123456789_1col_123456789_8doc-type:Articledoc-type:Textopen_accessstatus-type:publishedVersionddc:570
Kommerein, Nadine
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Stumpp, Sascha N.
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Musken, Matthias
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Ehlert, Nina
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Winkel, Andreas
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600
Haussler, Susanne
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Behrens, Peter
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Buettner, Falk F.R.
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Stiesch, Meike
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2017-04-06T06:44:27Z
2017-04-06T06:44:27Z
2017
Kommerein, N.; Stumpp, S.N.; Musken, M.; Ehlert, N.; Winkel, A. et al.: An oral multispecies biofilm model for high content screening applications. In: PLoS ONE 12 (2017), Nr. 3, 173973. DOI: https://doi.org/10.1371/journal.pone.0173973
http://www.repo.uni-hannover.de/handle/123456789/1296
http://dx.doi.org/10.15488/1271
Peri-implantitis caused by multispecies biofilms is a major complication in dental implant treatment. The bacterial infection surrounding dental implants can lead to bone loss and, in turn, to implant failure. A promising strategy to prevent these common complications is the development of implant surfaces that inhibit biofilm development. A reproducible and easyto-use biofilm model as a test system for large scale screening of new implant surfaces with putative antibacterial potency is therefore of major importance. In the present study, we developed a highly reproducible in vitro four-species biofilm model consisting of the highly relevant oral bacterial species Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar and Porphyromonas gingivalis. The application of live/dead staining, quantitative real time PCR (qRT-PCR), scanning electron microscopy (SEM) and urea-NaCl fluorescence in situ hybridization (urea-NaCl-FISH) revealed that the four-species biofilm community is robust in terms of biovolume, live/dead distribution and individual species distribution over time. The biofilm community is dominated by S. oralis, followed by V. dispar, A. naeslundii and P. gingivalis. The percentage distribution in this model closely reflects the situation in early native plaques and is therefore well suited as an in vitro model test system. Furthermore, despite its nearly native composition, the multispecies model does not depend on nutrient additives, such as native human saliva or serum, and is an inexpensive, easy to handle and highly reproducible alternative to the available model systems. The 96-well plate format enables high content screening for optimized implant surfaces impeding biofilm formation or the testing of multiple antimicrobial treatment strategies to fight multispecies biofilm infections, both exemplary proven in the manuscript.
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Previous issue date: 2017
VolkswagenStiftung/VWZN2860
DFG/EXC/REBIRTH
DFG/EXC/62/2
publishedVersion
eng
San Francisco, CA : Public Library of Science
PLoS ONE 12 (2017), Nr. 3
1932-6203
https://doi.org/10.1371/journal.pone.0173973
CC BY 4.0 Unported
https://creativecommons.org/licenses/by/4.0/
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
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An oral multispecies biofilm model for high content screening applications
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oai:www.repo.uni-hannover.de:123456789/1296
2022-12-02 17:17:36.888
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