Small sample stress: Probing oxygen-deprived ammonia-oxidizing bacteria with raman spectroscopy in vivo

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dc.identifier.uri http://dx.doi.org/10.15488/9891
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/9949
dc.contributor.author Kniggendorf, Ann-Kathrin
dc.contributor.author Nogueira, Regina
dc.contributor.author Bahmanabad, Somayeh Nasiri
dc.contributor.author Pommerening-Röser, Andreas
dc.contributor.author Roth, Bernhard Wilhelm
dc.date.accessioned 2020-06-29T15:21:46Z
dc.date.available 2020-06-29T15:21:46Z
dc.date.issued 2020
dc.identifier.citation Kniggendorf, A.-K.; Nogueira, R.; Bahmanabad, S.N.; Pommerening-Röser, A.; Roth, B.W.: Small sample stress: Probing oxygen-deprived ammonia-oxidizing bacteria with raman spectroscopy in vivo. In: Microorganisms 8 (2020), Nr. 3, 432. DOI: https://doi.org/10.3390/microorganisms8030432
dc.description.abstract The stress response of ammonia-oxidizing bacteria (AOB) to oxygen deprivation limits AOB growth and leads to different nitrification pathways that cause the release of greenhouse gases. Measuring the stress response of AOB has proven to be a challenge due to the low growth rates of stressed AOB, making the sample volumes required to monitor the internal stress response of AOB prohibitive to repeated analysis. In a proof-of-concept study, confocal Raman microscopy with excitation resonant to the heme c moiety of cytochrome c was used to compare the cytochrome c content and activity of stressed and unstressed Nitrosomonas europaea (Nm 50), Nitrosomonas eutropha (Nm 57), Nitrosospira briensis (Nsp 10), and Nitrosospira sp. (Nsp 02) in vivo. Each analysis required no more than 1000 individual cells per sampling; thus, the monitoring of cultures with low cell concentrations was possible. The identified spectral marker delivered reproducible results within the signal-to-noise ratio of the underlying Raman spectra. Cytochrome c content was found to be elevated in oxygen-deprived and previously oxygen-deprived samples. In addition, cells with predominantly ferrous cytochrome c content were found in deprived Nitrosomonas eutropha and Nitrosospira samples, which may be indicative of ongoing electron storage at the time of measurement. eng
dc.language.iso eng
dc.publisher Basel : MDPI AG
dc.relation.ispartofseries Microorganisms 8 (2020), Nr. 3
dc.relation.uri https://doi.org/10.3390/microorganisms8030432
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Confocal Raman microscopy eng
dc.subject Cytochrome c eng
dc.subject Nitrosomonas eng
dc.subject Nitrosospira eng
dc.subject Oxygen deprivation eng
dc.subject Resonance Raman spectroscopy eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Small sample stress: Probing oxygen-deprived ammonia-oxidizing bacteria with raman spectroscopy in vivo eng
dc.type article
dc.type Text
dc.relation.issn 2076-2607
dc.bibliographicCitation.issue 3
dc.bibliographicCitation.volume 8
dc.bibliographicCitation.firstPage 432
tib.accessRights frei zug�nglich


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