dc.identifier.uri |
http://dx.doi.org/10.15488/9360 |
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dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/9414 |
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dc.contributor.author |
Becker, Annegret
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dc.contributor.author |
Lehrich, Tina
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dc.contributor.author |
Kalies, Stefan
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dc.contributor.author |
Heisterkamp, Alexander
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dc.contributor.author |
Ngezahayo, Anaclet
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dc.date.accessioned |
2020-02-14T16:17:26Z |
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dc.date.available |
2020-02-14T16:17:26Z |
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dc.date.issued |
2019 |
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dc.identifier.citation |
Becker, A. et al.: Parameters for Optoperforation-Induced Killing of Cancer Cells Using Gold Nanoparticles Functionalized With the C-terminal Fragment of Clostridium Perfringens Enterotoxin. In: International Journal of Molecular Sciences 20 (2019), Nr. 17, 4248. DOI: https://doi.org/10.3390/ijms20174248 |
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dc.description.abstract |
Recently, we used a recombinant produced C-terminus (D194-F319) of the Clostridium perfringens enterotoxin (C-CPE) to functionalize gold nanoparticles (AuNPs) for a subsequent specific killing of claudin expressing tumor cells using the gold nanoparticle-mediated laser perforation (GNOME-LP) technique. For a future in vivo application, it will be crucial to know the physical parameters and the biological mechanisms inducing cell death for a rational adaptation of the system to real time situation. Regarding the AuNP functionalization, we observed that a relationship of 2.5 × 10−11 AuNP/mL to 20 µg/mL C-CPE maximized the killing efficiency. Regardingphysical parameters, a laser fluence up to 30 mJ/cm2 increased the killing efficiency. Independent from the applied laser fluence, the maximal killing efficiency was achieved at a scanning velocity of 5 mm/s. In 3D matrigel culture system, the GNOME-LP/C-CPE-AuNP completely destroyed spheroids composed of Caco-2 cells and reduced OE-33 cell spheroid formation. At the biology level, GNOME-LP/C-CPE-AuNP-treated cells bound annexin V and showed reduced mitochondria activity. However, an increased caspase-3/7 activity in the cells was not found. Similarly, DNA analysis revealed no apoptosis-related DNA ladder. The results suggest that the GNOME-LP/C-CPE-AuNP treatment induced necrotic than apoptotic reaction in tumor cells. |
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dc.language.iso |
eng |
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dc.publisher |
Basel : MDPI |
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dc.relation.ispartofseries |
International Journal of Molecular Sciences 20 (2019), Nr. 17 |
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dc.rights |
CC BY 4.0 Unported |
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dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
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dc.subject |
C-CPE |
eng |
dc.subject |
gold nanoparticle |
eng |
dc.subject |
GNOME-LP |
eng |
dc.subject |
apoptosis |
eng |
dc.subject |
necrosis |
eng |
dc.subject.ddc |
570 | Biowissenschaften, Biologie
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dc.subject.ddc |
610 | Medizin, Gesundheit
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dc.title |
Parameters for Optoperforation-Induced Killing of Cancer Cells Using Gold Nanoparticles Functionalized With the C-terminal Fragment of Clostridium Perfringens Enterotoxin |
eng |
dc.type |
Article |
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dc.type |
Text |
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dc.relation.doi |
10.3390/ijms20174248 |
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dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
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