O-Methylation steps during strobilurin and bolineol biosynthesis

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dc.identifier.uri http://dx.doi.org/10.15488/9357
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/9411
dc.contributor.author Lebe, Karen E. ger
dc.contributor.author Cox, Russell J. ger
dc.date.accessioned 2020-02-14T11:32:37Z
dc.date.available 2020-02-14T11:32:37Z
dc.date.issued 2019
dc.identifier.citation Lebe, K.E.; Cox, R.J.: O-Methylation steps during strobilurin and bolineol biosynthesis. In: RSC Advances 54 (2019), 31527. DOI: https://doi.org/10.1039/c9ra06412e ger
dc.description.abstract Strobilurins are potent antifungal polyketides produced by basidiomycete fungi. Two genes encoding O-methyltransferases (O-MeT) are present in the biosynthetic gene cluster of strobilurin A 1. In previous studies, the two O-MeT enzymes Str2 and Str3 were found to catalyse the final steps of the biosynthesis of 1. Here, we show by in vivo expression experiments, that O-methylation during strobilurin biosynthesis is regiospecific. O-MeT Str2 acts first and selectively catalyses the methylation of the carboxyl group of strobilurin and bolineol precursors. Str3 catalyses the subsequent methyl transfer to the enol group to form strobilurin A 1, but cannot methylate bolineol 4. Toxicity tests showed increasing antifungal activity of intermediates through the pathway and that bolineol 4 shows antifungal activity against A. oryzae NSAR1 with an MIC of 0.1 mg ml−1. ger
dc.language.iso eng ger
dc.publisher London : RSC
dc.relation.ispartofseries RSC Advances 54 (2019) ger
dc.rights CC BY-NC 3.0 Unported ger
dc.rights.uri https://creativecommons.org/licenses/by-nc/3.0/ ger
dc.subject Strobilurins eng
dc.subject O-MeT eng
dc.subject gene cluster eng
dc.subject.ddc 540 | Chemie ger
dc.title O-Methylation steps during strobilurin and bolineol biosynthesis ger
dc.type article ger
dc.type Text ger
dc.relation.doi 10.1039/c9ra06412e
dc.description.version publishedVersion ger

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